Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,904,553 T→A 100% P111P (CCA→CCT)  pstS ← periplasmic phosphate binding protein, high‑affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,904,5530TA98.9% 615.6 / ‑2.3 187P111P (CCA→CCT) pstSperiplasmic phosphate binding protein, high‑affinity
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (105/80);  total (107/80)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

GCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGGAA  >  CP009273/3904409‑3904697
                                                                                                                                                |                                                                                                                                                
gcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGaa                                                                                                                                              <  2:2840199/149‑1 (MQ=255)
      tacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTaa                                                                                                                                        <  1:3949692/149‑1 (MQ=255)
          ncAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                    >  1:3615643/2‑149 (MQ=255)
          gCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                    >  2:740959/1‑149 (MQ=255)
          gCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                    >  1:3191922/1‑149 (MQ=255)
          gCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                    >  1:1504204/1‑149 (MQ=255)
          gCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                                    >  2:2805220/1‑149 (MQ=255)
              tGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGc                                                                                                                                >  1:3040819/1‑149 (MQ=255)
               gTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGca                                                                                                                               >  2:2285129/1‑149 (MQ=255)
               gTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGca                                                                                                                               >  2:2285132/1‑149 (MQ=255)
                ttttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcac                                                                                                                              >  1:2065203/1‑149 (MQ=255)
                 tttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcacc                                                                                                                             <  2:268734/149‑1 (MQ=255)
                  ttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcacca                                                                                                                            <  1:196433/149‑1 (MQ=255)
                         ggCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACgccgcc                                                                                                                     <  2:2042438/149‑1 (MQ=255)
                          gCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCa                                                                                                                    >  1:2018769/1‑149 (MQ=255)
                             gTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATc                                                                                                                 <  1:1593914/149‑1 (MQ=255)
                                 cAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCTCTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACgg                                                                                                             <  2:114472/149‑1 (MQ=255)
                                   gACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTc                                                                                                           >  1:2630320/1‑149 (MQ=255)
                                    aCCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCg                                                                                                          >  2:261658/1‑149 (MQ=255)
                                    aCCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCg                                                                                                          >  1:455509/1‑149 (MQ=255)
                                        ggATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGac                                                                                                      >  2:4011588/1‑148 (MQ=255)
                                        ggATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGaa                                                                                                      >  1:618053/1‑149 (MQ=255)
                                        ggATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGaa                                                                                                      >  1:2761746/1‑149 (MQ=255)
                                         gATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAAc                                                                                                     >  1:2904318/1‑149 (MQ=255)
                                          aTTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACt                                                                                                    >  2:3287624/1‑149 (MQ=255)
                                           ttCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTg                                                                                                   <  2:2160431/149‑1 (MQ=255)
                                            tCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTgg                                                                                                  >  1:3438490/1‑149 (MQ=255)
                                            tCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTgg                                                                                                  <  1:580450/149‑1 (MQ=255)
                                            tCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTgg                                                                                                  <  1:108169/149‑1 (MQ=255)
                                                  tGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGa                                                                                            <  1:2929582/149‑1 (MQ=255)
                                                   ggCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGAc                                                                                           >  1:2679702/1‑149 (MQ=255)
                                                      gATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGACCAGACCtt                                                                                        >  2:2147359/1‑149 (MQ=255)
                                                          gCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTg                                                                                    >  2:407685/1‑149 (MQ=255)
                                                          gCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTg                                                                                    <  1:1312717/149‑1 (MQ=255)
                                                           cTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGa                                                                                   <  1:2737845/149‑1 (MQ=255)
                                                               atcatcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCa                                                                               <  2:1629920/149‑1 (MQ=255)
                                                                tcatcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                              <  1:516662/149‑1 (MQ=255)
                                                                tcatcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                              >  2:1823501/1‑149 (MQ=255)
                                                                  atcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtt                                                                            <  1:4094657/149‑1 (MQ=255)
                                                                  atcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTCACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtt                                                                            <  1:772846/149‑1 (MQ=255)
                                                                   tcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                           <  1:3736867/149‑1 (MQ=255)
                                                                   tcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                           <  1:825372/149‑1 (MQ=255)
                                                                   tcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                           >  1:3329093/1‑149 (MQ=255)
                                                                     ccACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttttt                                                                         >  2:715383/1‑149 (MQ=255)
                                                                        cttcttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGt                                                                      >  1:2837091/1‑149 (MQ=255)
                                                                        cttcttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGt                                                                      >  2:1959276/1‑149 (MQ=255)
                                                                         ttcttGATTTTGCCCAGGTAGATGTAGCCGAGGGTTTTACCATCCAGCACCAGATCGCCAGACTTAAGCCCAGGAATGTTAACAGCCAGCACCACGCCTCCAATCACGGTCGGGAAATGGAACAGACCTACCTGAGCCAGTTTTTCGTc                                                                     >  2:1490338/1‑149 (MQ=255)
                                                                            ttGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGTCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGa                                                                  >  2:3036680/1‑149 (MQ=255)
                                                                               aTTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACGTGGGGGCCCCTCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                               >  2:3916552/1‑149 (MQ=255)
                                                                               aTTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                               >  1:1445239/1‑149 (MQ=255)
                                                                               aTTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                               <  2:171777/149‑1 (MQ=255)
                                                                                  ttGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgcgg                                                            <  1:768751/149‑1 (MQ=255)
                                                                                        aGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTc                                                      <  2:2660581/149‑1 (MQ=255)
                                                                                         ggTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                                     >  2:2658551/1‑149 (MQ=255)
                                                                                         ggTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                                     >  2:1366765/1‑149 (MQ=255)
                                                                                          gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                                    >  1:3345752/1‑149 (MQ=255)
                                                                                           tAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCaga                                                   <  1:326287/149‑1 (MQ=255)
                                                                                            aGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCagag                                                  <  1:1937267/149‑1 (MQ=255)
                                                                                              aTGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGc                                                <  1:2516595/149‑1 (MQ=255)
                                                                                                 tCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCAcc                                             >  2:1578831/1‑149 (MQ=255)
                                                                                                 tCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCAcc                                             >  2:1050310/1‑149 (MQ=255)
                                                                                                     cGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCaaaa                                         <  1:3029005/149‑1 (MQ=255)
                                                                                                      gAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAAt                                        >  1:4078132/1‑149 (MQ=255)
                                                                                                      gAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAAt                                        >  1:3631282/1‑149 (MQ=255)
                                                                                                      gAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAAt                                        >  1:476093/1‑149 (MQ=255)
                                                                                                       aGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATc                                       >  1:2382313/1‑149 (MQ=255)
                                                                                                       aGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATc                                       >  2:616040/1‑149 (MQ=255)
                                                                                                         ggTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCaa                                     <  2:3660840/149‑1 (MQ=255)
                                                                                                          gTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAAc                                    >  1:1351025/1‑149 (MQ=255)
                                                                                                           ttttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACg                                   >  1:1086596/1‑149 (MQ=255)
                                                                                                            tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACgg                                  <  2:1144597/149‑1 (MQ=255)
                                                                                                             ttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGt                                 <  2:3615643/149‑1 (MQ=255)
                                                                                                              tACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTa                                <  1:1465342/149‑1 (MQ=255)
                                                                                                               aCCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGACTGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAt                               >  2:2560160/1‑149 (MQ=255)
                                                                                                               aCCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAt                               >  1:385046/1‑149 (MQ=255)
                                                                                                               aCCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAt                               <  1:2250753/149‑1 (MQ=255)
                                                                                                                ccATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAtt                              >  2:3546281/1‑149 (MQ=255)
                                                                                                                ccATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAtt                              <  2:2464860/149‑1 (MQ=255)
                                                                                                                  aTCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAg                            >  2:3627481/1‑149 (MQ=255)
                                                                                                                  aTCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAg                            >  2:3169148/1‑149 (MQ=255)
                                                                                                                   tCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGc                           >  2:4036025/1‑149 (MQ=255)
                                                                                                                       gCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATa                       >  1:1008907/1‑149 (MQ=255)
                                                                                                                        cACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATaa                      <  1:2336177/149‑1 (MQ=255)
                                                                                                                         aCCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGACCTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAAt                     >  2:1763638/1‑149 (MQ=255)
                                                                                                                          ccAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATc                    <  1:250608/149‑1 (MQ=255)
                                                                                                                           cAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCt                   <  2:3012125/149‑1 (MQ=255)
                                                                                                                           aatttCGCCAGACTTCAGCCCAGTAATGTTAACCGGCAGCACCACGCCGCCAATCTCGGTCGGGATGTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTGAGAGGGACCAAAATCAACGGTATTAGCGATAATCt                   <  2:824229/146‑1 (MQ=255)
                                                                                                                                cGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGtttt              <  1:740959/149‑1 (MQ=255)
                                                                                                                                cGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGtttt              <  2:3326553/149‑1 (MQ=255)
                                                                                                                                cGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGtttt              <  2:1274816/149‑1 (MQ=255)
                                                                                                                                  ccAGACTTCAGCCCAGGACTGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTAc            >  2:2995721/1‑149 (MQ=255)
                                                                                                                                  ccAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTAc            >  2:937894/1‑149 (MQ=255)
                                                                                                                                  ccAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTAc            <  2:3102747/149‑1 (MQ=255)
                                                                                                                                  ccAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTAc            >  2:1116104/1‑149 (MQ=255)
                                                                                                                                     gACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGcc         >  2:1006370/1‑149 (MQ=255)
                                                                                                                                     gACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGcc         >  1:1476782/1‑149 (MQ=255)
                                                                                                                                      aCTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCa        >  1:551004/1‑149 (MQ=255)
                                                                                                                                       cTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTAGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCAc       <  2:3458725/149‑1 (MQ=255)
                                                                                                                                          cAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCgg    <  2:2017873/149‑1 (MQ=255)
                                                                                                                                           aGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGga   >  2:151452/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCCACCGGAA  >  CP009273/3904409‑3904697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑