Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,416 C→A 100% R107L (CGT→CTT)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4160CA95.5% 589.2 / 17.5 198R107L (CGT→CTT) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base A (94/95);  total (98/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

TACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGA  >  CP009273/3910270‑3910561
                                                                                                                                                  |                                                                                                                                                 
tACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGc                                                                                                                                                 <  2:3124789/149‑1 (MQ=255)
 aCCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGct                                                                                                                                                >  1:2722110/1‑149 (MQ=255)
 aCCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGct                                                                                                                                                >  1:2619289/1‑149 (MQ=255)
  ccTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGctc                                                                                                                                               >  1:1044920/1‑149 (MQ=255)
     acacCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCtcttc                                                                                                                                            <  1:245597/149‑1 (MQ=255)
       acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTcttctt                                                                                                                                          >  1:3427203/1‑149 (MQ=255)
       acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTcttctt                                                                                                                                          >  1:1379154/1‑149 (MQ=255)
       acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTcttctt                                                                                                                                          >  2:3884314/1‑149 (MQ=255)
       acCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTcttctt                                                                                                                                          <  1:4007170/149‑1 (MQ=255)
          cGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCAc                                                                                                                                       <  2:228586/149‑1 (MQ=255)
               caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtc                                                                                                                                  <  1:228318/149‑1 (MQ=255)
               caccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGGTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtc                                                                                                                                  <  2:2555222/149‑1 (MQ=255)
                accGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtct                                                                                                                                 >  1:3008173/1‑149 (MQ=255)
                 ccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtctc                                                                                                                                <  2:1699353/149‑1 (MQ=255)
                 ccGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGAtctc                                                                                                                                >  1:2394924/1‑149 (MQ=255)
                   gAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGc                                                                                                                              >  2:800691/1‑149 (MQ=255)
                      gaGACCAACTTTTCCGCCCTTAGCGTACGGACACATCTGGTCGATATCTTTGATACCGGTTTCCAGCAGTTCCTGTGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGTTATCGCCtt                                                                                                                           <  2:1011722/148‑1 (MQ=255)
                       aGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCttt                                                                                                                          <  1:3931009/149‑1 (MQ=255)
                       aGACCAACTTTACCGCCCTGAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCttt                                                                                                                          <  1:2795337/149‑1 (MQ=255)
                        gACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTc                                                                                                                         <  1:1119192/149‑1 (MQ=255)
                         aCCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCa                                                                                                                        <  2:116880/149‑1 (MQ=255)
                           cAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATg                                                                                                                      <  1:1851510/149‑1 (MQ=255)
                             ncTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTc                                                                                                                    >  1:2855513/2‑149 (MQ=255)
                             aCTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTc                                                                                                                    >  2:1981928/1‑149 (MQ=255)
                             aCTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTc                                                                                                                    >  2:4062309/1‑149 (MQ=255)
                                ttACCGCCCTGAGCGAACGGACACATCAGGTCGATAACTTGGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAc                                                                                                                 <  2:1424728/149‑1 (MQ=255)
                                    cGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGt                                                                                                             <  2:2626585/149‑1 (MQ=255)
                                        cTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCAc                                                                                                         <  1:3757661/149‑1 (MQ=255)
                                           aGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCa                                                                                                      >  2:3585706/1‑149 (MQ=255)
                                            gCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                     >  1:603828/1‑149 (MQ=255)
                                            gCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                     >  1:831970/1‑149 (MQ=255)
                                              gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                   >  2:891700/1‑149 (MQ=255)
                                              gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                   >  2:1347726/1‑149 (MQ=255)
                                               aaCGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                  <  1:1132700/149‑1 (MQ=255)
                                                  ggACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGtt                                                                                               >  1:765751/1‑149 (MQ=255)
                                                    acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                             <  2:4013889/149‑1 (MQ=255)
                                                    acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                             <  1:3034826/149‑1 (MQ=255)
                                                     cacaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCAt                                                                                            >  1:3866379/1‑149 (MQ=255)
                                                      acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                           >  2:1595759/1‑149 (MQ=255)
                                                      acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                           >  2:1595559/1‑149 (MQ=255)
                                                      acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                           >  2:3922534/1‑149 (MQ=255)
                                                      acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                           >  1:453134/1‑149 (MQ=255)
                                                          cAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATAc                                                                                       <  2:2156057/149‑1 (MQ=255)
                                                           aGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACg                                                                                      <  2:2889819/149‑1 (MQ=255)
                                                           aGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACg                                                                                      >  2:1493534/1‑149 (MQ=255)
                                                            ggTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACgg                                                                                     >  1:3236192/1‑149 (MQ=255)
                                                              tCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGcc                                                                                   <  2:325819/149‑1 (MQ=255)
                                                              tCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGcc                                                                                   <  2:2065117/149‑1 (MQ=255)
                                                               cGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGccc                                                                                  <  2:1465889/149‑1 (MQ=255)
                                                                gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCCGTACGTTCATGATACGGCCCa                                                                                 >  2:1726923/1‑149 (MQ=255)
                                                                gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCa                                                                                 <  1:1391538/149‑1 (MQ=255)
                                                                  tAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCaga                                                                               >  2:606672/1‑149 (MQ=255)
                                                                    aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                             <  2:1065870/149‑1 (MQ=255)
                                                                    aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                             <  2:533898/149‑1 (MQ=255)
                                                                    aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                             <  1:3595403/149‑1 (MQ=255)
                                                                    aCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                             <  1:3309933/149‑1 (MQ=255)
                                                                     cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                            <  1:930581/149‑1 (MQ=255)
                                                                     cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                            >  2:1190549/1‑149 (MQ=255)
                                                                       ttGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                          <  1:2462974/149‑1 (MQ=255)
                                                                         gATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCtt                                                                        <  1:1728981/149‑1 (MQ=255)
                                                                           tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGcggtt                                                                      >  1:3702967/1‑145 (MQ=255)
                                                                           tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCgttt                                                                      >  1:836010/1‑148 (MQ=255)
                                                                           tACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCgttt                                                                      >  1:2015817/1‑148 (MQ=255)
                                                                            aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCttttc                                                                     >  1:745123/1‑147 (MQ=255)
                                                                              cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacct                                                                   <  1:1906971/149‑1 (MQ=255)
                                                                              cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacca                                                                   >  2:3504970/1‑148 (MQ=255)
                                                                              cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTGtacct                                                                   >  2:765173/1‑149 (MQ=255)
                                                                                      agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGAc                                                           <  2:1786989/149‑1 (MQ=255)
                                                                                      agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAGCTAACGGGAc                                                           >  1:1272301/1‑149 (MQ=255)
                                                                                      agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCAACCgcggc                                                           >  1:430920/1‑145 (MQ=255)
                                                                                       gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCAACCCGGACt                                                          >  2:2447264/1‑149 (MQ=255)
                                                                                         agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTc                                                        <  1:366657/149‑1 (MQ=255)
                                                                                           ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCaa                                                      <  2:3844550/149‑1 (MQ=255)
                                                                                            tCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAAt                                                     <  2:2575827/149‑1 (MQ=255)
                                                                                               tGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACAAACGGGACGTCAATCgg                                                  >  1:3386745/1‑149 (MQ=255)
                                                                                                  gagTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCCGGGCATCaaaacggtg                                               >  1:2228111/1‑142 (MQ=255)
                                                                                                     tttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                            <  2:2722110/149‑1 (MQ=255)
                                                                                                        gACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAg                                         <  1:1047367/149‑1 (MQ=255)
                                                                                                        gACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAAACCGGACATAAATCGGGTGTTGGAg                                         >  1:2846289/1‑149 (MQ=255)
                                                                                                          cAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGTCTTCAATCGGGTGTTCGCGGt                                       >  2:3686114/1‑149 (MQ=255)
                                                                                                              tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCttt                                   <  2:266903/149‑1 (MQ=255)
                                                                                                               ctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCtttt                                  <  2:256895/149‑1 (MQ=255)
                                                                                                                   gTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACAt                              <  1:1493468/149‑1 (MQ=255)
                                                                                                                    tAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAGCGACCCGGCCGGCAAGCGGGTGGTCGAGGTGTTTTTCATc                             >  1:3633999/1‑149 (MQ=255)
                                                                                                                      ggAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCACTCGGGTGTTCGAGGTCTTTTACATCCa                           >  2:2710471/1‑149 (MQ=255)
                                                                                                                        aaGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGa                         <  2:2520435/149‑1 (MQ=255)
                                                                                                                        aaGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCGGGACTTCAATCGGGGGTTCGAGGTCTTTTACATCCAGa                         >  1:3737278/1‑149 (MQ=255)
                                                                                                                         aGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGAc                        <  2:2023016/149‑1 (MQ=255)
                                                                                                                         aGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACTTAAATAGGGTGGTCGAGGTCTTTTAAATCCAGAc                        >  2:453358/1‑149 (MQ=255)
                                                                                                                          ggTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGAcc                       <  2:3732606/149‑1 (MQ=255)
                                                                                                                            tgctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACcgc                     <  2:1903680/149‑1 (MQ=255)
                                                                                                                                    ggTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGa             <  1:3357313/149‑1 (MQ=255)
                                                                                                                                      tGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAcc           >  2:2211659/1‑149 (MQ=255)
                                                                                                                                      tGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTGCGACCCGGCCTTGACAAGGGTGTTGGAGGTGTTTTTAATAAAGACCGAGACGCAGAcc           >  1:733905/1‑149 (MQ=255)
                                                                                                                                         aTCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCGACCGGGCCGTCAAACGGGTGGTGGTGGTGTTTTAAAAACAGACCCCGCCGCCGACCCTc        >  1:1971177/1‑149 (MQ=255)
                                                                                                                                            gCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgga     <  1:1434571/149‑1 (MQ=255)
                                                                                                                                            gCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAGCGACCGGGACATCCATCGGGTGTTCGAGGTCTTTTAAATCCAGACCCCGACGCAGACCGTCgga     >  1:1325399/1‑149 (MQ=255)
                                                                                                                                               cAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCAAACCGGCCGTCAATAGGGTGTTAGAGGTGTTTTAAATAAACACCCCGACGCAGACCCTCggaggt  >  1:3217981/1‑148 (MQ=255)
                                                                                                                                               cAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCCGGACTTAAAAAGGGTGTTAGTGGTCTTTTACATCACGACCGCGACCCACACCGTCggcgga  >  1:1731693/1‑149 (MQ=255)
                                                                                                                                               cAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCggagga  <  2:2362927/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGA  >  CP009273/3910270‑3910561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑