Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,931,021 C→A 100% A123E (GCG→GAG)  rbsK → ribokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,931,0210CA97.7% 737.1 / 6.2 237A123E (GCG→GAG) rbsKribokinase
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (108/123);  total (112/125)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

CCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTC  >  CP009273/3930875‑3931164
                                                                                                                                                  |                                                                                                                                               
ccAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGTCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                               <  1:2249566/149‑1 (MQ=255)
ccAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                               <  1:805888/149‑1 (MQ=255)
ccAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGGGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGgagc                                                                                                                                               <  1:2341245/149‑1 (MQ=255)
 cagcagCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCt                                                                                                                                              >  1:3336780/1‑149 (MQ=255)
  agcagcTCGCCACTGATAACATTGATATGACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtg                                                                                                                                             <  1:1071449/149‑1 (MQ=255)
   gcagcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtgg                                                                                                                                            >  2:2101377/1‑149 (MQ=255)
     agcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggtg                                                                                                                                          <  2:2058772/149‑1 (MQ=255)
     agcTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCtggtg                                                                                                                                          <  2:3482686/149‑1 (MQ=255)
         cGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAg                                                                                                                                      <  2:3942815/149‑1 (MQ=255)
              cTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCaa                                                                                                                                 >  1:4040139/1‑149 (MQ=255)
                 aTAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                              >  2:1781653/1‑149 (MQ=255)
                 aTAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                              >  2:3822400/1‑149 (MQ=255)
                 aTAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGt                                                                                                                              <  1:3003328/149‑1 (MQ=255)
                  tAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTg                                                                                                                             >  2:2905260/1‑149 (MQ=255)
                   aaCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                            <  1:1024451/149‑1 (MQ=255)
                   aaCATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGa                                                                                                                            <  2:1883279/149‑1 (MQ=255)
                     cATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGc                                                                                                                          >  1:3819527/1‑149 (MQ=255)
                       ttGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGt                                                                                                                        >  1:554534/1‑149 (MQ=255)
                          atatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                     <  1:63102/149‑1 (MQ=255)
                          atatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                     >  1:2806703/1‑149 (MQ=255)
                          atatTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTAtt                                                                                                                     <  2:3782201/149‑1 (MQ=255)
                            atTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGc                                                                                                                   >  2:84560/1‑149 (MQ=255)
                             ttACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGcc                                                                                                                  >  2:2590029/1‑149 (MQ=255)
                               aCTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCaa                                                                                                                <  2:3192957/149‑1 (MQ=255)
                                      tCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCa                                                                                                         >  2:471167/1‑149 (MQ=255)
                                      tCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCAACGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTCAGCGTATTGCCAACGCGTcc                                                                                                         >  1:961399/1‑148 (MQ=255)
                                        aGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGc                                                                                                       <  2:532507/149‑1 (MQ=255)
                                         gCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGTCGAATGTGAGAATGTCATCGGTATTCATGACGGCGCTAATTCTGACCTTTCCCAGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGACATCa                                                                                                      >  1:2181907/1‑149 (MQ=255)
                                         gCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCa                                                                                                      >  2:1399029/1‑149 (MQ=255)
                                            tGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCatta                                                                                                   <  1:3237420/149‑1 (MQ=255)
                                              aTCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCattatt                                                                                                 >  1:953474/1‑149 (MQ=255)
                                                cAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTaa                                                                                               <  2:1630058/149‑1 (MQ=255)
                                                 aaaGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAAt                                                                                              <  2:566957/149‑1 (MQ=255)
                                                  aaGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATg                                                                                             <  1:2225040/149‑1 (MQ=255)
                                                  aaGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATg                                                                                             <  1:479493/149‑1 (MQ=255)
                                                   aGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGc                                                                                            >  2:2161433/1‑149 (MQ=255)
                                                    ggCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCa                                                                                           <  1:275339/149‑1 (MQ=255)
                                                      cGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGc                                                                                         <  1:3081025/149‑1 (MQ=255)
                                                      cGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGc                                                                                         <  1:2478202/149‑1 (MQ=255)
                                                       gAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCt                                                                                        <  2:1180527/149‑1 (MQ=255)
                                                        aaTCAACAGGTGTGGCGCTTATTTTTGTTAATGGCGAAGGTGAGAATTTCATCGGAATTCAAGCCGGCGCTAATTATGACCATTCCCCGGAGCTGGTGGAAGCTCAACGTGACCGTATTGCCAACGCCTCACCATTATTAATGCATCTg                                                                                       >  1:2587475/1‑149 (MQ=255)
                                                        aaTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTg                                                                                       <  1:3163973/149‑1 (MQ=255)
                                                         aTCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACCTGAGCGTATTGCCAACGCGTCAGCATTATTAATTCAGCTgg                                                                                      >  1:1752219/1‑149 (MQ=255)
                                                          tCACCAGGTGTGGCGCCGAGTAGTGTTACTGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGa                                                                                     <  2:3941913/149‑1 (MQ=255)
                                                          tCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGa                                                                                     <  2:680030/149‑1 (MQ=255)
                                                           cAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGaa                                                                                    <  2:2712124/149‑1 (MQ=255)
                                                            aaCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAAt                                                                                   >  2:2007139/1‑149 (MQ=255)
                                                             aCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATc                                                                                  >  1:4112815/1‑149 (MQ=255)
                                                             aCAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATc                                                                                  >  2:1915085/1‑149 (MQ=255)
                                                               aGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcac                                                                                >  2:2277302/1‑149 (MQ=255)
                                                                 gtgtGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcacca                                                                              <  2:1673672/149‑1 (MQ=255)
                                                                  tgtgGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATcaccac                                                                             <  1:1916346/149‑1 (MQ=255)
                                                                     ggCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCg                                                                          >  1:2054548/1‑149 (MQ=255)
                                                                         cagATTTTTGTTAATGCCGAAGGTGAGAATGTCATCGGTATTCATGCAGGCGCTAATGCTGCCATTAACCCGGAGCTGGTGGAAGCTCAACGTGAGAGTAGTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACAACTCGAAAg                                                                      >  2:2544962/3‑149 (MQ=255)
                                                                           gATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAgtg                                                                    <  1:2383956/149‑1 (MQ=255)
                                                                              ttttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGa                                                                 <  1:1003294/149‑1 (MQ=255)
                                                                               tttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGAt                                                                <  1:1366235/149‑1 (MQ=255)
                                                                                ttGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATg                                                               <  2:2944238/149‑1 (MQ=255)
                                                                                 tGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATgg                                                              <  2:439287/149‑1 (MQ=255)
                                                                                   ttAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCa                                                            >  2:232507/1‑149 (MQ=255)
                                                                                     aaTGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgc                                                          >  1:2913839/1‑149 (MQ=255)
                                                                                        ggCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAgcggc                                                       >  2:758878/1‑149 (MQ=255)
                                                                                              ggTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAAt                                                 >  1:3866273/1‑149 (MQ=255)
                                                                                              ggTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAAt                                                 >  2:664725/1‑149 (MQ=255)
                                                                                               gTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATc                                                >  1:3587004/1‑149 (MQ=255)
                                                                                               gTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATc                                                <  1:1790788/149‑1 (MQ=255)
                                                                                                 gagaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGc                                              >  2:2637111/1‑149 (MQ=255)
                                                                                                 gagaATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGc                                              >  1:3914910/1‑149 (MQ=255)
                                                                                                      tGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATc                                         <  2:2220332/149‑1 (MQ=255)
                                                                                                      tGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATc                                         >  2:919950/1‑149 (MQ=255)
                                                                                                      tGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATc                                         >  2:1209830/1‑149 (MQ=255)
                                                                                                          aTCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCaaaa                                     <  1:3895640/149‑1 (MQ=255)
                                                                                                           tCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAAt                                    >  2:1099416/1‑149 (MQ=255)
                                                                                                               tATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGa                                >  2:3766707/1‑149 (MQ=255)
                                                                                                               tATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGa                                >  2:1071498/1‑149 (MQ=255)
                                                                                                               tATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGa                                <  1:4101105/149‑1 (MQ=255)
                                                                                                               tATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGa                                >  2:1071473/1‑149 (MQ=255)
                                                                                                                aTTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGAc                               <  1:3470163/149‑1 (MQ=255)
                                                                                                                 ttCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACt                              >  1:884608/1‑149 (MQ=255)
                                                                                                                  tCATGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTa                             <  2:1337522/149‑1 (MQ=255)
                                                                                                                   cATGCCGGCGCTAATGCTACCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTAt                            >  2:1350386/1‑149 (MQ=255)
                                                                                                                    aTGCCGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATc                           >  2:3545240/1‑149 (MQ=255)
                                                                                                                       ccGGCGCTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGtt                        <  1:690642/149‑1 (MQ=255)
                                                                                                                            gcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCt                   >  2:2412863/1‑149 (MQ=255)
                                                                                                                            gcTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCt                   <  1:3822406/149‑1 (MQ=255)
                                                                                                                             cTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCtt                  <  2:3816496/149‑1 (MQ=255)
                                                                                                                             cTAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCtt                  >  1:249360/1‑149 (MQ=255)
                                                                                                                              tAATGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTa                 <  1:1794206/149‑1 (MQ=255)
                                                                                                                               aaTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTaa                <  2:616553/149‑1 (MQ=255)
                                                                                                                                aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAc               <  1:2947630/149‑1 (MQ=255)
                                                                                                                                aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAc               >  1:2631366/1‑149 (MQ=255)
                                                                                                                                aTGCTGCCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGAGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAc               >  1:2630203/1‑149 (MQ=255)
                                                                                                                                 tgctgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAcc              <  2:2379788/149‑1 (MQ=255)
                                                                                                                                  gctgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAAccc             <  1:811230/149‑1 (MQ=255)
                                                                                                                                    tgcCCTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccgg           >  2:3718514/1‑149 (MQ=255)
                                                                                                                                       ccTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctc        <  2:4040139/149‑1 (MQ=255)
                                                                                                                                       ccTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctc        >  1:3250720/1‑149 (MQ=255)
                                                                                                                                       ccTTTCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctc        <  1:2022411/149‑1 (MQ=255)
                                                                                                                                        cTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctcc       >  2:270317/1‑149 (MQ=255)
                                                                                                                                         tttCCCCGGAGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACccggctccg      >  2:925138/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CCAGCAGCTCGCCACTGATAACATTGATATTACTCCGGTCAGCGTGATCAAAGGCGAATCAACAGGTGTGGCGCTGATTTTTGTTAATGGCGAAGGTGAGAATGTCATCGGTATTCATGCCGGCGCTAATGCTGCCCTTTCCCCGGCGCTGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTC  >  CP009273/3930875‑3931164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑