Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,959,351 G→T 100% P77P (CCG→CCT)  trxA → thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,959,3510GT99.6% 835.7 / ‑4.4 252P77P (CCG→CCT) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (129/121);  total (130/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

TGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCCG  >  CP009273/3959205‑3959496
                                                                                                                                                  |                                                                                                                                                 
   gCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActct                                                                                                                                              <  1:1451959/149‑1 (MQ=255)
   gCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActct                                                                                                                                              <  2:3901453/149‑1 (MQ=255)
   gCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActct                                                                                                                                              <  2:3895745/149‑1 (MQ=255)
     agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                            <  1:1240066/149‑1 (MQ=255)
     agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                            >  1:3927050/1‑149 (MQ=255)
        gtggtgCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgctgc                                                                                                                                         <  2:1443375/149‑1 (MQ=255)
             gCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTc                                                                                                                                    <  2:1894048/149‑1 (MQ=255)
             gCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTc                                                                                                                                    <  2:3619184/149‑1 (MQ=255)
               ggTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaa                                                                                                                                  <  1:884309/149‑1 (MQ=255)
                  ccGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaaa                                                                                                                               <  1:1375198/149‑1 (MQ=255)
                    gTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                             >  2:2897260/1‑149 (MQ=255)
                      gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                           <  2:703386/149‑1 (MQ=255)
                      gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                           <  2:1053005/149‑1 (MQ=255)
                        aaaaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGa                                                                                                                         >  1:2066200/1‑149 (MQ=255)
                        aaaaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGa                                                                                                                         <  1:3717429/149‑1 (MQ=255)
                        aaaaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGa                                                                                                                         <  1:2023006/149‑1 (MQ=255)
                          aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAg                                                                                                                       >  1:3467483/1‑149 (MQ=255)
                            tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                     >  2:1833270/1‑149 (MQ=255)
                            tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                     <  1:2469723/149‑1 (MQ=255)
                            tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                     <  1:1010513/149‑1 (MQ=255)
                            tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                     >  2:2263853/1‑149 (MQ=255)
                               tCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcg                                                                                                                  >  2:2157026/1‑149 (MQ=255)
                                  ccccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCa                                                                                                               >  2:828822/1‑149 (MQ=255)
                                    ccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAc                                                                                                             >  1:3546320/1‑149 (MQ=255)
                                      gATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCa                                                                                                           <  2:3228401/149‑1 (MQ=255)
                                       aTTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaa                                                                                                          >  1:1500127/1‑149 (MQ=255)
                                        ttCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGTACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaaa                                                                                                         <  2:1593277/149‑1 (MQ=255)
                                            ggATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTgg                                                                                                     <  2:3963359/149‑1 (MQ=255)
                                            ggATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTgg                                                                                                     >  1:2002752/1‑149 (MQ=255)
                                            ggATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTgg                                                                                                     >  1:1207866/1‑149 (MQ=255)
                                                  aaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCAc                                                                                               <  1:2419631/149‑1 (MQ=255)
                                                   aTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACt                                                                                              >  1:433789/1‑149 (MQ=255)
                                                   aTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACt                                                                                              >  1:26268/1‑149 (MQ=255)
                                                    tCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTg                                                                                             >  1:3412879/1‑149 (MQ=255)
                                                     cGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGt                                                                                            <  1:593815/149‑1 (MQ=255)
                                                         gACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                        <  2:1762164/149‑1 (MQ=255)
                                                         gACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                        >  1:3149993/1‑149 (MQ=255)
                                                         gACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                        >  1:2470605/1‑149 (MQ=255)
                                                          aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                       <  1:3203530/149‑1 (MQ=255)
                                                          aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                       <  2:619888/149‑1 (MQ=255)
                                                           cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAg                                                                                      >  2:314757/1‑149 (MQ=255)
                                                           cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAg                                                                                      >  2:931603/1‑149 (MQ=255)
                                                              atatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTCTCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                   >  2:3987472/1‑149 (MQ=255)
                                                                atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                                 >  2:1141065/1‑149 (MQ=255)
                                                                atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                                 <  1:1266056/149‑1 (MQ=255)
                                                                   aGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTg                                                                              >  1:2461101/1‑149 (MQ=255)
                                                                    gggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGa                                                                             >  2:2088227/1‑149 (MQ=255)
                                                                     ggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaa                                                                            >  1:1703333/1‑149 (MQ=255)
                                                                     ggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaa                                                                            >  2:2796375/1‑149 (MQ=255)
                                                                      gCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGaaa                                                                           <  2:1086386/149‑1 (MQ=255)
                                                                           cTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAATCGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGtt                                                                      <  2:1231819/149‑1 (MQ=255)
                                                                             gACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                    >  1:4057774/1‑149 (MQ=255)
                                                                              aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCt                                                                   <  2:3870218/149‑1 (MQ=255)
                                                                                 gTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                                <  1:1682966/149‑1 (MQ=255)
                                                                                 gTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                                <  1:3961986/149‑1 (MQ=255)
                                                                                  ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                               <  1:792408/149‑1 (MQ=255)
                                                                                  ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                               >  1:735717/1‑149 (MQ=255)
                                                                                      aaaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTa                                                           >  1:1773499/1‑149 (MQ=255)
                                                                                      aaaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTa                                                           >  1:1729215/1‑149 (MQ=255)
                                                                                       aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTaa                                                          >  2:2932513/1‑149 (MQ=255)
                                                                                       aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTaa                                                          <  2:3668307/149‑1 (MQ=255)
                                                                                       aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTaa                                                          >  2:1206923/1‑149 (MQ=255)
                                                                                         aCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAcc                                                        <  1:3019060/149‑1 (MQ=255)
                                                                                           tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTg                                                      <  2:1176021/149‑1 (MQ=255)
                                                                                            gAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                     >  1:3865810/1‑149 (MQ=255)
                                                                                              aCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                   <  2:1733177/149‑1 (MQ=255)
                                                                                              aCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                   >  2:302685/1‑149 (MQ=255)
                                                                                               cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                  <  2:380335/149‑1 (MQ=255)
                                                                                               cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                  >  2:3150979/1‑149 (MQ=255)
                                                                                               cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                  >  1:1876721/1‑149 (MQ=255)
                                                                                                     tCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                            >  2:4112485/1‑149 (MQ=255)
                                                                                                       aaaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAt                                          <  1:2790761/149‑1 (MQ=255)
                                                                                                        aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                         >  2:805661/1‑149 (MQ=255)
                                                                                                        aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCACTACTCTGCTGCTGTTCAAAAACTGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                         >  1:357042/1‑149 (MQ=255)
                                                                                                          aCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTc                                       <  1:3042555/149‑1 (MQ=255)
                                                                                                             cTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                    >  2:2824627/1‑149 (MQ=255)
                                                                                                             cTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                    >  2:3319464/1‑149 (MQ=255)
                                                                                                              tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGt                                   >  2:2272609/1‑149 (MQ=255)
                                                                                                               ggCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGtt                                  <  1:1837472/149‑1 (MQ=255)
                                                                                                               ggCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGtt                                  >  1:2258706/1‑149 (MQ=255)
                                                                                                                   cTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCggg                              <  2:3553886/149‑1 (MQ=255)
                                                                                                                    tGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGt                             <  2:4089807/149‑1 (MQ=255)
                                                                                                                     gcgcCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTg                            <  1:1098/149‑1 (MQ=255)
                                                                                                                      cgcCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGc                           <  1:829051/149‑1 (MQ=255)
                                                                                                                        ccGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGccc                         <  2:2586011/149‑1 (MQ=255)
                                                                                                                           aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGt                      >  1:2976979/1‑149 (MQ=255)
                                                                                                                           aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGt                      >  2:54346/1‑149 (MQ=255)
                                                                                                                           aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGt                      >  1:4015150/1‑149 (MQ=255)
                                                                                                                              tatGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGc                   <  1:3600883/149‑1 (MQ=255)
                                                                                                                                tGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTAGCTa                 >  2:2389287/1‑149 (MQ=255)
                                                                                                                                  gCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaa               >  2:2332516/1‑149 (MQ=255)
                                                                                                                                    aTCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaa             >  1:351456/1‑149 (MQ=255)
                                                                                                                                         tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGa        <  2:3734564/149‑1 (MQ=255)
                                                                                                                                         tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGa        >  2:3490913/1‑149 (MQ=255)
                                                                                                                                          ggTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGAc       <  2:1717701/149‑1 (MQ=255)
                                                                                                                                             aTCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc    >  2:3581077/1‑149 (MQ=255)
                                                                                                                                             aTCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc    <  2:1309815/149‑1 (MQ=255)
                                                                                                                                             aTCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc    >  1:921568/1‑149 (MQ=255)
                                                                                                                                              tCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc   <  2:732354/149‑1 (MQ=255)
                                                                                                                                               cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCCg  <  2:2663313/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCCG  >  CP009273/3959205‑3959496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑