Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,967,474 G→T 100% A169A (GCG→GCT)  rffH → glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,967,4740GT99.1% 739.7 / ‑1.7 224A169A (GCG→GCT) rffHglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (114/108);  total (114/110)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 228 total aligned reads displayed.

CGTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGG  >  CP009273/3967328‑3967615
                                                                                                                                                  |                                                                                                                                             
cGTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGt                                                                                                                                             <  1:2895843/149‑1 (MQ=255)
    aTGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGAcc                                                                                                                                         >  2:1422354/1‑149 (MQ=255)
    aTGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGAcc                                                                                                                                         >  2:1422376/1‑149 (MQ=255)
      gTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCgg                                                                                                                                       <  1:331951/149‑1 (MQ=255)
       ttGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCggg                                                                                                                                      <  1:4058621/149‑1 (MQ=255)
       ttGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCggg                                                                                                                                      <  1:3390610/149‑1 (MQ=255)
         gcggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTGGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCt                                                                                                                                    <  1:3888783/149‑1 (MQ=255)
          cggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCtt                                                                                                                                   <  1:1219296/149‑1 (MQ=255)
          cggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCtt                                                                                                                                   <  2:3043710/149‑1 (MQ=255)
           ggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                  <  1:348916/149‑1 (MQ=255)
             cgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAt                                                                                                                                <  1:2230439/149‑1 (MQ=255)
              gcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAtt                                                                                                                               <  2:785999/149‑1 (MQ=255)
              gcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAtt                                                                                                                               <  2:2382924/149‑1 (MQ=255)
              gcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAtt                                                                                                                               >  2:2975724/1‑149 (MQ=255)
               cgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAttt                                                                                                                              <  2:1053674/149‑1 (MQ=255)
               cgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTAttt                                                                                                                              <  2:96178/149‑1 (MQ=255)
                gcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTc                                                                                                                             >  2:1281824/1‑149 (MQ=255)
                 cACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCt                                                                                                                            >  2:434333/1‑149 (MQ=255)
                  aCCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTa                                                                                                                           >  2:3576231/1‑149 (MQ=255)
                  aCCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTa                                                                                                                           >  1:288720/1‑149 (MQ=255)
                     gAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGa                                                                                                                        >  1:1842798/1‑149 (MQ=255)
                        ggggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAg                                                                                                                     <  2:3801383/149‑1 (MQ=255)
                        ggggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAg                                                                                                                     >  2:2781731/1‑149 (MQ=255)
                         gggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGt                                                                                                                    >  2:2342791/1‑149 (MQ=255)
                             gACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                <  2:3931348/149‑1 (MQ=255)
                             gACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                <  1:226687/149‑1 (MQ=255)
                              aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                               <  1:2347940/149‑1 (MQ=255)
                              aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                               <  1:3751359/149‑1 (MQ=255)
                               cGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTc                                                                                                              <  2:4083851/149‑1 (MQ=255)
                                  ttttgGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTg                                                                                                           <  2:721334/149‑1 (MQ=255)
                                              aGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAg                                                                                               <  1:18129/149‑1 (MQ=255)
                                                gTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCa                                                                                             <  1:1246453/149‑1 (MQ=255)
                                                gTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCa                                                                                             <  1:2135253/149‑1 (MQ=255)
                                                 tGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAg                                                                                            >  2:490220/1‑149 (MQ=255)
                                                  gATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAgg                                                                                           <  1:2732650/149‑1 (MQ=255)
                                                  gATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAgg                                                                                           >  2:3119752/1‑149 (MQ=255)
                                                        cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGc                                                                                     <  2:2675429/149‑1 (MQ=255)
                                                        cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGc                                                                                     <  2:2202784/149‑1 (MQ=255)
                                                        cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGc                                                                                     <  1:576637/149‑1 (MQ=255)
                                                            gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                 >  2:2319790/1‑149 (MQ=255)
                                                            gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                 >  1:518864/1‑149 (MQ=255)
                                                            gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                 <  1:1153259/149‑1 (MQ=255)
                                                            gAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                 <  2:3607984/149‑1 (MQ=255)
                                                             aaCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCg                                                                                >  2:663289/1‑149 (MQ=255)
                                                              aCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCgg                                                                               >  2:2245054/1‑149 (MQ=255)
                                                                gCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAg                                                                             >  2:4004342/1‑149 (MQ=255)
                                                                  tttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCg                                                                           >  1:842346/1‑149 (MQ=255)
                                                                   ttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCgt                                                                          <  2:10053/149‑1 (MQ=255)
                                                                   ttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCgt                                                                          <  1:2176723/149‑1 (MQ=255)
                                                                     ggCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCgtgg                                                                        >  2:1789291/1‑149 (MQ=255)
                                                                          ggtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAAc                                                                   <  1:3155191/149‑1 (MQ=255)
                                                                          ggtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAAc                                                                   >  2:1895221/1‑149 (MQ=255)
                                                                            tggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTg                                                                 >  2:1886839/1‑149 (MQ=255)
                                                                            tggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTg                                                                 <  1:1597498/149‑1 (MQ=255)
                                                                             ggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTgg                                                                <  2:3096112/149‑1 (MQ=255)
                                                                                gTTTGACTACTATTTCCGCGCTATCTCGCTGGTAGAAAAGACAAAACAGCCGAATTCAAACTGGGCTGTGAGCGGGCTTTTTTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgaga                                                             <  1:744082/149‑1 (MQ=255)
                                                                                gTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgaga                                                             <  1:744552/149‑1 (MQ=255)
                                                                                  ttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGAtt                                                           <  2:2585828/149‑1 (MQ=255)
                                                                                     acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                        >  2:4114374/1‑149 (MQ=255)
                                                                                     acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                        >  1:2723783/1‑149 (MQ=255)
                                                                                     acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                        >  1:3654205/1‑149 (MQ=255)
                                                                                     acgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                        <  2:7651/149‑1 (MQ=255)
                                                                                       gacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTc                                                      >  1:1069465/1‑149 (MQ=255)
                                                                                          aaTTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCAt                                                   >  1:2567447/1‑149 (MQ=255)
                                                                                           aTTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATc                                                  >  1:2007474/1‑149 (MQ=255)
                                                                                             ttCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAACGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCaa                                                <  1:897110/149‑1 (MQ=255)
                                                                                             ttCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTAGTGAACTGGAGATTACCTCCATCaa                                                <  2:2454722/149‑1 (MQ=255)
                                                                                                cgcgCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCa                                             <  2:590719/149‑1 (MQ=255)
                                                                                                  cgcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGa                                           <  2:3638183/149‑1 (MQ=255)
                                                                                                      aTCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTa                                       >  1:1011198/1‑149 (MQ=255)
                                                                                                      aTCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTa                                       >  2:2705278/1‑149 (MQ=255)
                                                                                                      aTCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTa                                       >  1:1008429/1‑149 (MQ=255)
                                                                                                       tctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTAc                                      >  1:1486082/1‑149 (MQ=255)
                                                                                                       tctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTAc                                      >  1:1463923/1‑149 (MQ=255)
                                                                                                       tctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTAc                                      >  2:3771002/1‑149 (MQ=255)
                                                                                                         tcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCt                                    >  1:882041/1‑149 (MQ=255)
                                                                                                            cTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTCCCTCGa                                 >  2:448855/1‑149 (MQ=255)
                                                                                                             tGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                <  1:1422354/149‑1 (MQ=255)
                                                                                                              ggAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAgg                               >  2:2409317/1‑149 (MQ=255)
                                                                                                               gaagaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGc                              >  2:1650616/1‑149 (MQ=255)
                                                                                                                  gaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCggg                           >  1:1366263/1‑149 (MQ=255)
                                                                                                                   aaaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCTACCAGATGTACCTCGAGGCGGGc                          >  2:280729/1‑149 (MQ=255)
                                                                                                                     aaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCaa                        >  1:684060/1‑149 (MQ=255)
                                                                                                                       gCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAAcc                      >  2:1164819/1‑149 (MQ=255)
                                                                                                                       gCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAAcc                      >  1:1791675/1‑149 (MQ=255)
                                                                                                                          aaaaCAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGa                   >  2:2001570/1‑149 (MQ=255)
                                                                                                                           aaaCAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGAc                  >  1:2441701/1‑149 (MQ=255)
                                                                                                                               aGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGtt              >  1:3589954/1‑149 (MQ=255)
                                                                                                                                gCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTg             >  1:38205/1‑149 (MQ=255)
                                                                                                                                   gAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAAc          <  2:1613106/149‑1 (MQ=255)
                                                                                                                                      gTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGc       >  1:1494620/1‑149 (MQ=255)
                                                                                                                                      gTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGc       >  2:3547977/1‑149 (MQ=255)
                                                                                                                                      gTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGc       >  2:888916/1‑149 (MQ=255)
                                                                                                                                       tCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCt      <  1:2531268/149‑1 (MQ=255)
                                                                                                                                         aaaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCg    >  1:486623/1‑149 (MQ=255)
                                                                                                                                          aaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCgg   >  1:219958/1‑149 (MQ=255)
                                                                                                                                          aaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCgg   >  1:936940/1‑149 (MQ=255)
                                                                                                                                          aaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTAAGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCgg   >  1:427406/1‑149 (MQ=255)
                                                                                                                                           aCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCggg  <  2:3054227/149‑1 (MQ=255)
                                                                                                                                           aCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCggg  <  2:924288/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CGTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGG  >  CP009273/3967328‑3967615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑