BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,988,075	T→A	100%	V65E (GTG→GAG)	yifL →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,988,075	0	T	A	100.0%	1018.8 / NA	303	V65E (GTG→GAG)	yifL	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (144/159); total (144/159)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

CCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTG  >  CP009273/3987929‑3988220

 cTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAACCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                                <  1:934795/149‑1 (MQ=255)

 cTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                                <  2:3046420/149‑1 (MQ=255)

 cTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGaa                                                                                                                                                <  1:1695025/149‑1 (MQ=255)

  tGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAt                                                                                                                                               <  1:3604261/149‑1 (MQ=255)

   gACGGGCTGCGGTCTGAATGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGTTAGAGACCCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAAtt                                                                                                                                              <  1:3536470/149‑1 (MQ=255)

    aCGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTa                                                                                                                                             <  1:2289408/149‑1 (MQ=255)

      gggCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACt                                                                                                                                           >  1:705474/1‑149 (MQ=255)

        gCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTaa                                                                                                                                         >  2:880810/1‑149 (MQ=255)

           gCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAg                                                                                                                                      <  1:509353/149‑1 (MQ=255)

                cTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGt                                                                                                                                 <  1:1239542/149‑1 (MQ=255)

                  gAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGttt                                                                                                                               >  1:1055756/1‑149 (MQ=255)

                   aaaGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTc                                                                                                                              <  1:3350227/149‑1 (MQ=255)

                        tCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCACGAGAATTACTAAAAGTCAGTTTATGTAc                                                                                                                         >  2:2109329/1‑149 (MQ=255)

                                aTTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGa                                                                                                                 >  1:420648/1‑149 (MQ=255)

                                aTTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGa                                                                                                                 >  2:678069/1‑149 (MQ=255)

                                aTTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGa                                                                                                                 >  2:486193/1‑149 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCTAACCTGTTGTGACGCAAACGCAATCCACGGTGCCTGATAAACACGATCGCGCCACTGGCGATGGTCCATCCCTGGAGAATGACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                <  2:1366594/149‑1 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCGGTTTCTGTACCCGCGTGAt                                                                                                                >  2:1872799/1‑149 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                >  2:2632148/1‑149 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                >  2:3624525/1‑149 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                >  2:1552283/1‑149 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                <  1:3013417/149‑1 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                <  2:1225753/149‑1 (MQ=255)

                                 tttCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAt                                                                                                                >  2:567522/1‑149 (MQ=255)

                                         cTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaa                                                                                                        >  2:820333/1‑149 (MQ=255)

                                          tGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTaaa                                                                                                       >  2:655509/1‑149 (MQ=255)

                                            cAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCTATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatg                                                                                                     <  1:3472488/149‑1 (MQ=255)

                                             aGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAatga                                                                                                    <  1:2551149/149‑1 (MQ=255)

                                               aTAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGt                                                                                                                                 >  2:177590/1‑118 (MQ=255)

                                                tAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGAGTGGAGTAAATGATGc                                                                                                 >  2:3325942/1‑149 (MQ=255)

                                                 aaaaaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCa                                                                                                >  2:2141044/1‑149 (MQ=255)

                                                    aaCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGtt                                                                                             <  2:3819840/149‑1 (MQ=255)

                                                     aCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                            <  1:3787604/149‑1 (MQ=255)

                                                     aCGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTtc                                                                                            <  2:3567646/149‑1 (MQ=255)

                                                                ccgACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTCCCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCa                                                                                 >  2:237776/1‑149 (MQ=255)

                                                                  gACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                               <  1:2483982/149‑1 (MQ=255)

                                                                  gACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATg                                                                               >  2:2076304/1‑149 (MQ=255)

                                                                   aCCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATgg                                                                              >  2:3126539/1‑149 (MQ=255)

                                                                   aCCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATgg                                                                              >  2:2766311/1‑149 (MQ=255)

                                                                   aCCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATgg                                                                              >  1:1595208/1‑149 (MQ=255)

                                                                     cAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGcc                                                                            >  2:3181382/1‑149 (MQ=255)

                                                                      aaaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCt                                                                           <  1:3561237/149‑1 (MQ=255)

                                                                       acccGGTAGAGACGCAAACGCAATCCCCGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                          <  2:2443854/147‑1 (MQ=255)

                                                                       aaCCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCtt                                                                          >  2:1128181/1‑149 (MQ=255)

                                                                          cGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGc                                                                       <  1:2028622/149‑1 (MQ=255)

                                                                            gTAGAGACGAAACCGCAATCCACGGTGCCGGATAAAAACGCTCGCGCCACTGGCGAGGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCaa                                                                     <  1:1868707/149‑1 (MQ=255)

                                                                             tAGAGACGCAAACGCACTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAAc                                                                    <  1:440380/149‑1 (MQ=255)

                                                                             tAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAAc                                                                    <  1:3158746/149‑1 (MQ=255)

                                                                                 gaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtt                                                                <  2:2947133/149‑1 (MQ=255)

                                                                                 gaCGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAtt                                                                <  1:390510/149‑1 (MQ=255)

                                                                                  acgcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTACAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGAttt                                                               <  1:2419844/149‑1 (MQ=255)

                                                                                    gcaaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCACCGATTTTa                                                             >  1:3052146/1‑149 (MQ=255)

                                                                                     caaacgcaaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTAt                                                            <  1:3667059/149‑1 (MQ=255)

                                                                                           caaTCCACGGTGCCGGATAAACACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATGACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgt                                                      <  2:1154689/149‑1 (MQ=255)

                                                                                           caaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGgtcgt                                                      >  2:764670/1‑149 (MQ=255)

                                                                                            aaTCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGACAATGCATGGCCTTGGCAACGATTTTATGgtcgtc                                                     >  2:3965172/1‑149 (MQ=255)

                                                                                              tCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGa                                                   <  1:1453725/149‑1 (MQ=255)

                                                                                              tCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGa                                                   <  1:1289397/149‑1 (MQ=255)

                                                                                               ccACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAc                                                  <  2:2297993/149‑1 (MQ=255)

                                                                                                 aCGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgc                                                <  2:2843743/149‑1 (MQ=255)

                                                                                                  cGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGAcgcg                                               >  1:2660782/1‑149 (MQ=255)

                                                                                                    gTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAACAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGt                                             <  1:2190185/149‑1 (MQ=255)

                                                                                                     tGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTa                                            >  1:1322002/1‑149 (MQ=255)

                                                                                                      gCCGGATAAACACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTaa                                           <  1:3218106/149‑1 (MQ=255)

                                                                                                      gCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTaa                                           <  1:1795019/149‑1 (MQ=255)

                                                                                                      gCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTaa                                           <  2:1676507/149‑1 (MQ=255)

                                                                                                          gATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCa                                       <  1:1366445/149‑1 (MQ=255)

                                                                                                            tAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                     >  1:2822970/1‑149 (MQ=255)

                                                                                                            tAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGa                                     >  1:578298/1‑149 (MQ=255)

                                                                                                             aaaaaCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGaa                                    <  1:2972685/149‑1 (MQ=255)

                                                                                                                 aCGATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTc                                >  1:1003772/1‑149 (MQ=255)

                                                                                                                   gATCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtt                              <  1:1716909/149‑1 (MQ=255)

                                                                                                                    aTCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttt                             <  1:2772454/149‑1 (MQ=255)

                                                                                                                    aTCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCACTTTCTGTACCCCCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCttt                             >  1:4035301/1‑149 (MQ=255)

                                                                                                                     tCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                            <  2:2855801/149‑1 (MQ=255)

                                                                                                                     tCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                            <  2:518442/149‑1 (MQ=255)

                                                                                                                     tCGCGCCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttt                            <  2:1421126/149‑1 (MQ=255)

                                                                                                                       gcgcCACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCtttttt                          <  1:938636/149‑1 (MQ=255)

                                                                                                                          ccACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                       >  2:1745254/1‑149 (MQ=255)

                                                                                                                          ccACTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCa                       >  2:1744198/1‑149 (MQ=255)

                                                                                                                            aCTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAcc                     >  2:3461328/1‑149 (MQ=255)

                                                                                                                            aCTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCAcc                     >  2:2420585/1‑149 (MQ=255)

                                                                                                                            aCTGGCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAAGGTCTTTTTTTCAcc                     >  2:3927474/1‑149 (MQ=255)

                                                                                                                               ggCGATGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGa                  <  1:3128371/149‑1 (MQ=255)

                                                                                                                                 cgttGGTCCATCGCAGGAGTATTACTATAAGTCAGTTTCTGTACCCGCGTGATTGGTGTAAATGATGCAGTTCTCGATATTGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCCCCGGAGc                <  2:1258729/146‑1 (MQ=255)

                                                                                                                                   aTGGTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTg              <  2:441219/149‑1 (MQ=255)

                                                                                                                                     ggTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAt            <  1:844850/149‑1 (MQ=255)

                                                                                                                                     ggTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAt            >  1:1777606/1‑149 (MQ=255)

                                                                                                                                     ggTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAt            <  2:103825/149‑1 (MQ=255)

                                                                                                                                      gTCCATCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGAtt           >  2:219097/1‑149 (MQ=255)

                                                                                                                                          aTCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcgtc       <  1:654112/149‑1 (MQ=255)

                                                                                                                                          aTCCCAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATtcgtc       <  1:1592557/149‑1 (MQ=255)

                                                                                                                                            cccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc     <  1:1515297/149‑1 (MQ=255)

                                                                                                                                            cccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGc     >  2:1800651/1‑149 (MQ=255)

                                                                                                                                             ccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGcc    <  1:1128181/149‑1 (MQ=255)

                                                                                                                                             ccAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGcc    <  2:1489970/149‑1 (MQ=255)

                                                                                                                                              cAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt   <  2:4072174/149‑1 (MQ=255)

                                                                                                                                              cAGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCt   <  2:260620/149‑1 (MQ=255)

                                                                                                                                               aGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTg  >  1:2753892/1‑149 (MQ=255)

                                                                                                                                               aGGAGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTg  >  1:1999810/1‑149 (MQ=255)

CCTGACGGGCTGCGGTCTGAAAGGTCCGCTCTATTTCCCGCCTGCAGATAAAAACGCACCGCCGCCGACCAAACCGGTAGAGACGCAAACGCAATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTACCCGCGTGATTGGAGTAAATGATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACCGGAGCTGATTCGTCGCCTG  >  CP009273/3987929‑3988220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`