BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,994,247	A→T	100%	V57E (GTG→GAG)	yigE ←	DUF2233 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,994,247	0	A	T	100.0%	873.4 / NA	260	V57E (GTG→GAG)	yigE	DUF2233 family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (113/147); total (113/147)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

CGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTT  >  CP009273/3994101‑3994392

cGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTcc                                                                                                                                                 <  1:4001778/149‑1 (MQ=255)

cGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTcc                                                                                                                                                 <  2:3142501/149‑1 (MQ=255)

   tGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGc                                                                                                                                              >  1:3620974/1‑149 (MQ=255)

     tACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGctc                                                                                                                                            <  1:2886023/149‑1 (MQ=255)

      aCAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGctct                                                                                                                                           <  2:1668959/149‑1 (MQ=255)

      aCAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTGTTTGCCAGTACATTTTCTCCCGcgct                                                                                                                                           >  2:3359306/1‑149 (MQ=255)

        aaaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGt                                                                                                                                         >  1:2180250/1‑149 (MQ=255)

         aaCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGtt                                                                                                                                        <  1:2996954/149‑1 (MQ=255)

          aCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                       >  2:3998119/1‑149 (MQ=255)

          aCCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGttt                                                                                                                                       <  2:2814181/149‑1 (MQ=255)

           ccGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTg                                                                                                                                      <  1:641548/149‑1 (MQ=255)

               gcggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGa                                                                                                                                  <  1:1194810/149‑1 (MQ=255)

                cggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAt                                                                                                                                 <  1:4089413/149‑1 (MQ=255)

                 ggcgCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGAtt                                                                                                                                <  1:3100412/149‑1 (MQ=255)

                   cgcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTaa                                                                                                                              <  2:3303979/149‑1 (MQ=255)

                   cgcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTaa                                                                                                                              <  2:1439322/149‑1 (MQ=255)

                    gcATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAAc                                                                                                                             >  1:2677485/1‑149 (MQ=255)

                       tAGCTTTCATCATAGATGCCGCCGTTCATGGCCATCTGCACCTTACCGTGACTATTAATATCCGCCACCAGTGCATGTAACGTTCCCAACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGt                                                                                                                          <  2:3177907/149‑1 (MQ=255)

                         gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                        <  1:2047486/149‑1 (MQ=255)

                         gCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                        >  1:2011959/1‑149 (MQ=255)

                         gCTTTCATCATAGATGCCGCCATTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGtat                                                                                                                        <  1:865461/149‑1 (MQ=255)

                           tttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATAc                                                                                                                      <  1:2432966/149‑1 (MQ=255)

                           tttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATAc                                                                                                                      >  1:1642469/1‑149 (MQ=255)

                           tttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAATAATATCCCCCAGCAGAGCAAGTAACGTTCCCCACGCATCGCCATTGGCTTTTTGCCAGTAAATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATAc                                                                                                                      >  1:1413135/1‑149 (MQ=255)

                            ttCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCCTTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACg                                                                                                                     >  1:1906267/1‑149 (MQ=255)

                             tcatcaTAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGc                                                                                                                    >  1:623806/1‑149 (MQ=255)

                               atcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                  <  2:244006/149‑1 (MQ=255)

                               atcatAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCt                                                                                                                  <  2:3013559/149‑1 (MQ=255)

                                   tAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTAc                                                                                                              <  1:261842/149‑1 (MQ=255)

                                      aTGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGt                                                                                                           >  1:833377/1‑149 (MQ=255)

                                        gccgccGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCa                                                                                                         <  2:4070454/149‑1 (MQ=255)

                                         ccgccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAg                                                                                                        <  2:2115265/149‑1 (MQ=255)

                                         ccgccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAg                                                                                                        <  2:932903/149‑1 (MQ=255)

                                            ccgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCTCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGt                                                                                                     <  1:27635/149‑1 (MQ=255)

                                             cgTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTc                                                                                                    >  2:2644403/1‑149 (MQ=255)

                                                   tCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCt                                                                                              <  1:963030/149‑1 (MQ=255)

                                                     gCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTga                                                                                            >  1:3830617/1‑149 (MQ=255)

                                                     gCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTga                                                                                            <  2:3033185/149‑1 (MQ=255)

                                                        aTCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGagag                                                                                         >  2:3771930/1‑149 (MQ=255)

                                                        aTCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGagag                                                                                         >  1:1487542/1‑149 (MQ=255)

                                                                ctgaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAat                                                                                 <  2:2733388/149‑1 (MQ=255)

                                                                  gaccctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAatca                                                                               <  1:3382822/149‑1 (MQ=255)

                                                                     cctgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAAtcatca                                                                            <  1:68287/149‑1 (MQ=255)

                                                                       tgacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGc                                                                          >  1:2210086/1‑149 (MQ=255)

                                                                        gacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACTTTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCTCAATCATCAGCg                                                                         >  2:3907474/1‑149 (MQ=255)

                                                                        gacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCg                                                                         <  1:232402/149‑1 (MQ=255)

                                                                        gacTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCg                                                                         <  2:3803595/149‑1 (MQ=255)

                                                                          cTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGc                                                                       <  1:705322/149‑1 (MQ=255)

                                                                            aTTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCaa                                                                     <  1:1251109/149‑1 (MQ=255)

                                                                            aTTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCaa                                                                     <  1:3042911/149‑1 (MQ=255)

                                                                               aaTATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAg                                                                  >  2:2825440/1‑149 (MQ=255)

                                                                                atatCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGc                                                                 <  1:2994765/149‑1 (MQ=255)

                                                                                 tatCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCa                                                                >  2:3293928/1‑149 (MQ=255)

                                                                                 tatCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCa                                                                >  2:3603298/1‑149 (MQ=255)

                                                                                  atCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCaa                                                               <  1:153511/149‑1 (MQ=255)

                                                                                   tCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCaaa                                                              <  1:2653090/149‑1 (MQ=255)

                                                                                      gCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACaa                                                           <  1:2368770/149‑1 (MQ=255)

                                                                                          gcagAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGa                                                       <  2:4042681/149‑1 (MQ=255)

                                                                                            agagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAg                                                     >  2:1947392/1‑149 (MQ=255)

                                                                                             gagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                    <  2:1521975/149‑1 (MQ=255)

                                                                                             gagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                    >  2:2505060/1‑149 (MQ=255)

                                                                                             gagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGt                                                    <  1:3264005/149‑1 (MQ=255)

                                                                                             gagCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGACAACAGCAAACAAGGGAAGt                                                    >  1:3441907/1‑149 (MQ=255)

                                                                                                 aTGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTCAgg                                                >  2:3290727/1‑149 (MQ=255)

                                                                                                 aTGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAgg                                                <  2:2057645/149‑1 (MQ=255)

                                                                                                  tGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGt                                               >  2:430359/1‑149 (MQ=255)

                                                                                                    tAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGa                                             <  2:3830617/149‑1 (MQ=255)

                                                                                                    tAACGGTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGa                                             <  1:2122967/149‑1 (MQ=255)

                                                                                                     aaCGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAg                                            <  1:4100264/149‑1 (MQ=255)

                                                                                                        gTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGc                                         >  2:2545384/1‑149 (MQ=255)

                                                                                                             ccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaa                                    >  1:916982/1‑149 (MQ=255)

                                                                                                             ccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaa                                    >  1:1367265/1‑149 (MQ=255)

                                                                                                               aCGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaa                                  <  2:1621650/149‑1 (MQ=255)

                                                                                                                cGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGaaaaa                                 >  1:3660930/1‑149 (MQ=255)

                                                                                                                 gCTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAAt                                >  1:529355/1‑149 (MQ=255)

                                                                                                                  cTTCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATc                               <  2:3674372/149‑1 (MQ=255)

                                                                                                                    tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCg                             >  1:2486114/1‑149 (MQ=255)

                                                                                                                    tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCg                             <  1:3919782/149‑1 (MQ=255)

                                                                                                                    tCGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCg                             <  1:3919824/149‑1 (MQ=255)

                                                                                                                     cGCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGt                            <  2:2329121/149‑1 (MQ=255)

                                                                                                                      gCCATTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGtt                           <  2:1112662/149‑1 (MQ=255)

                                                                                                                         aTTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                        <  1:3704090/149‑1 (MQ=255)

                                                                                                                         aTTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                        >  2:271387/1‑149 (MQ=255)

                                                                                                                         aTTGGCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTga                        >  1:108670/1‑149 (MQ=255)

                                                                                                                            ggCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                     >  2:3112783/1‑149 (MQ=255)

                                                                                                                            ggCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAt                     >  2:195832/1‑149 (MQ=255)

                                                                                                                             gCTTTTTGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGAtt                    >  2:3987832/1‑149 (MQ=255)

                                                                                                                                   tGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGt              <  1:1759422/149‑1 (MQ=255)

                                                                                                                                   tGCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGt              <  1:1274936/149‑1 (MQ=255)

                                                                                                                                    gCCAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTg             >  2:2023682/1‑149 (MQ=255)

                                                                                                                                     ccAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGa            <  1:2445219/149‑1 (MQ=255)

                                                                                                                                     ccAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGa            <  2:1740910/149‑1 (MQ=255)

                                                                                                                                     ccAGTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCACAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGa            >  2:2970999/1‑149 (MQ=255)

                                                                                                                                        gTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCa         <  2:3711888/149‑1 (MQ=255)

                                                                                                                                        gTACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCa         <  2:505676/149‑1 (MQ=255)

                                                                                                                                         tACATTTTCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCAt        <  1:3155872/149‑1 (MQ=255)

                                                                                                                                             ttttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCt    <  2:455287/149‑1 (MQ=255)

                                                                                                                                             ttttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCt    <  1:104052/149‑1 (MQ=255)

                                                                                                                                              tttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCtt   <  2:1697998/149‑1 (MQ=255)

                                                                                                                                               ttCTCCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCttt  <  2:813387/149‑1 (MQ=255)

CGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTT  >  CP009273/3994101‑3994392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`