Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,996,346 T→A 100% I70F (ATC→TTC)  yigG ← PRK11371 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,996,3460TA99.0% 669.2 / ‑2.4 207I70F (ATC→TTC) yigGPRK11371 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (106/99);  total (108/99)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

AAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAAT  >  CP009273/3996200‑3996491
                                                                                                                                                  |                                                                                                                                                 
 aGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAgaagaa                                                                                                                                                >  1:2714951/1‑149 (MQ=255)
    aTTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAAcc                                                                                                                                             >  1:4023054/1‑149 (MQ=255)
     ttAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                            >  1:1119683/1‑149 (MQ=255)
      tAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCAc                                                                                                                                           <  1:2969432/149‑1 (MQ=255)
      tAGCATACTGAAAGTGGAAAAATAAAAAATCAAAAAAATCATCGAACCATGGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCAc                                                                                                                                           <  1:387287/149‑1 (MQ=255)
                aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                 >  2:1561509/1‑149 (MQ=255)
                 aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                                <  1:2199897/149‑1 (MQ=255)
                    tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGc                                                                                                                             >  2:1942406/1‑149 (MQ=255)
                    tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGc                                                                                                                             >  1:822955/1‑149 (MQ=255)
                     ggAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCt                                                                                                                            >  1:1804991/1‑149 (MQ=255)
                     ggAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCt                                                                                                                            >  2:622423/1‑149 (MQ=255)
                       aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTgg                                                                                                                          <  1:1294900/149‑1 (MQ=255)
                          aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                       >  2:3110205/1‑149 (MQ=255)
                          aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                       >  1:4097550/1‑149 (MQ=255)
                            tAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAtt                                                                                                                     >  2:4049450/1‑149 (MQ=255)
                             aaCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTc                                                                                                                    <  2:860198/149‑1 (MQ=255)
                              aCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCa                                                                                                                   <  1:3508875/149‑1 (MQ=255)
                                 aaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                                <  2:2788704/149‑1 (MQ=255)
                                 aaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                                >  1:1895483/1‑149 (MQ=255)
                                  aTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGtt                                                                                                               >  2:3919098/1‑149 (MQ=255)
                                  aTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGtt                                                                                                               >  1:3433860/1‑149 (MQ=255)
                                     aaaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgat                                                                                                            >  2:3448887/1‑149 (MQ=255)
                                       aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatga                                                                                                          >  1:3364283/1‑149 (MQ=255)
                                         aaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACg                                                                                                        <  2:2640778/149‑1 (MQ=255)
                                          aaTCATCGAACCAGTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACga                                                                                                       <  2:229497/149‑1 (MQ=255)
                                           atcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgag                                                                                                      <  2:3902949/149‑1 (MQ=255)
                                             catcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCCATTCATGTTGATGACGAGAt                                                                                                    >  1:3295429/1‑149 (MQ=255)
                                             catcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCAGTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGAGGACGAGAt                                                                                                    >  2:1897151/1‑149 (MQ=255)
                                              atcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATa                                                                                                   <  1:543517/149‑1 (MQ=255)
                                              atcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATa                                                                                                   <  1:422495/149‑1 (MQ=255)
                                              atcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATa                                                                                                   >  2:2400366/1‑149 (MQ=255)
                                                   aCCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGa                                                                                              <  1:1717551/149‑1 (MQ=255)
                                                   aCCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGAGAATGGa                                                                                              >  2:126052/1‑149 (MQ=255)
                                                          ccTGAACAGGCAAAATCTTCGGCTATCATTGTTATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGAt                                                                                       <  2:1139154/149‑1 (MQ=255)
                                                            tGAACCGGCAAAATCATCGGCTATCAATGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACCTAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATgg                                                                                     <  2:2111791/149‑1 (MQ=255)
                                                             gAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGt                                                                                    <  2:86443/149‑1 (MQ=255)
                                                                cAGGCAAACTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTaga                                                                                 <  1:2123704/149‑1 (MQ=255)
                                                                cAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTaga                                                                                 <  1:2123591/149‑1 (MQ=255)
                                                                 aGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTCTCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTagag                                                                                >  2:855131/1‑149 (MQ=255)
                                                                   gCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAc                                                                              <  1:911832/149‑1 (MQ=255)
                                                                     aaaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACta                                                                            >  2:3527558/1‑149 (MQ=255)
                                                                      aaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTTGAGACtat                                                                           >  2:1844363/1‑149 (MQ=255)
                                                                        aTCTTCGTCTTTAATTGTGTTTATAGAGATGATATATACTGCTATTGTACCAAAAACATATGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATaa                                                                         <  2:2982454/149‑1 (MQ=255)
                                                                         tCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                        <  2:1895483/149‑1 (MQ=255)
                                                                          cTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACa                                                                       >  2:1086018/1‑149 (MQ=255)
                                                                              ggCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGaa                                                                   <  1:2165394/149‑1 (MQ=255)
                                                                               gCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAAAAGaaa                                                                  >  2:3822156/1‑149 (MQ=255)
                                                                                 tATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGTTGAGGGGATAATGGTGTAAGATGGTAGTTACTATAACAAGAAAGc                                                                >  1:371402/1‑149 (MQ=255)
                                                                                 tATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATTACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                                >  1:886277/1‑149 (MQ=255)
                                                                                 tATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                                >  1:2750959/1‑149 (MQ=255)
                                                                                 tATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                                <  2:3962378/149‑1 (MQ=255)
                                                                                   tCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACAATAACAAGAAATCCt                                                              >  1:2069464/1‑149 (MQ=255)
                                                                                      ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCt                                                           >  2:834555/1‑149 (MQ=255)
                                                                                        gtgATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATTGAGTACGATGGTAGATAATATAAAAAGAAAGCCTGCTtc                                                         >  1:565327/1‑149 (MQ=255)
                                                                                        gtgATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCtta                                                         <  2:2714951/149‑2 (MQ=255)
                                                                                         tgatgaTAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtct                                                        >  1:326658/1‑149 (MQ=255)
                                                                                           atgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTcc                                                      <  2:2050865/149‑1 (MQ=255)
                                                                                            tgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCa                                                     <  1:3118861/149‑1 (MQ=255)
                                                                                               tAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAATCCTGCTTCTCCATCg                                                  >  2:2604009/1‑149 (MQ=255)
                                                                                                 gagaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGtt                                                <  2:1677179/149‑1 (MQ=255)
                                                                                                    atgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTtaa                                             >  2:70635/1‑149 (MQ=255)
                                                                                                    atgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaa                                             >  2:2147454/1‑149 (MQ=255)
                                                                                                     tgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaa                                            <  2:3356389/149‑1 (MQ=255)
                                                                                                       atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                          <  2:45572/149‑1 (MQ=255)
                                                                                                       atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                          <  1:2273925/149‑1 (MQ=255)
                                                                                                         atataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGa                                        <  1:2920157/149‑1 (MQ=255)
                                                                                                          tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGat                                       <  1:1742802/149‑1 (MQ=255)
                                                                                                          tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGat                                       <  1:2853915/149‑1 (MQ=255)
                                                                                                          tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGat                                       <  1:1745323/149‑1 (MQ=255)
                                                                                                           ataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCCTGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGata                                      >  1:3615774/1‑149 (MQ=255)
                                                                                                           ataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGata                                      <  1:1422010/149‑1 (MQ=255)
                                                                                                            taCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataa                                     <  2:4023054/149‑1 (MQ=255)
                                                                                                              cTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataata                                   >  2:734440/1‑149 (MQ=255)
                                                                                                               tGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataa                                  <  1:2519699/149‑1 (MQ=255)
                                                                                                               tGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAACAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataa                                  >  2:1788307/1‑149 (MQ=255)
                                                                                                                gCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGGAATTGTTGTTGTTGTGGAGTTTTTTGTGTACGATGGTTGTGTCTATAACAAGAAATCCTGCTTGTCCATCGTTAAAAAATttaattat                                 >  1:4024408/1‑146 (MQ=255)
                                                                                                                 cTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                                >  2:294664/1‑149 (MQ=255)
                                                                                                                 cTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGa                                >  2:3717692/1‑149 (MQ=255)
                                                                                                                  tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaa                               <  2:1466409/149‑1 (MQ=255)
                                                                                                                   aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaaa                              <  2:3020872/149‑1 (MQ=255)
                                                                                                                    aTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAg                             >  2:653123/1‑149 (MQ=255)
                                                                                                                     tGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAgg                            <  1:3582385/149‑1 (MQ=255)
                                                                                                                     tGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAgg                            <  1:2672916/149‑1 (MQ=255)
                                                                                                                      gTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGc                           <  1:99275/149‑1 (MQ=255)
                                                                                                                       tACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAATATAATAAGAAAGGCa                          >  1:2236543/1‑149 (MQ=255)
                                                                                                                        aCCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGCGATAATTGAGTAAGATGGTAGAGACTATAACAACAAACCCAGCGTCTCCAACGTTAAAAAAGATAATAAGAAAGGCaa                         >  1:788384/1‑149 (MQ=255)
                                                                                                                          cAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAATATAATAAGAAAGGCaaaa                       >  2:3887798/1‑149 (MQ=255)
                                                                                                                                cATAATTTTTTATATAGAAGAAACCTCTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaaa                 <  2:1799107/149‑1 (MQ=255)
                                                                                                                                  tAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGGTAAAAAAGATAATAAGAAAGGCCAaaattaaatt               >  1:4004436/1‑149 (MQ=255)
                                                                                                                                  tAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATTGAGTACGATGGTAGAGACTATAACAAGAAATCCTGCTTCTCCATCGTTAAAAAATATAATAAGAAAGGCAAaaattaaatt               >  1:1965223/1‑149 (MQ=255)
                                                                                                                                  tAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATTTTTATTACGAGCTAATTGAGTACGATGGTAGAGACTATAACAAGAAAGCCAGCTTATCCAACGATAAAAAAGATAATAATAAAAGCAAAAATTaaaat               >  1:1396415/1‑147 (MQ=255)
                                                                                                                                   aaGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATTGAGTACGATGGTAGAGACTATAACAAGAAATCCTGCTTCTCCATCGTTAAAAAAGATAATAATAAAGGCAAAAAAGAAAttt              >  2:3143211/1‑149 (MQ=255)
                                                                                                                                   aaGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAAttt              <  1:3575467/149‑1 (MQ=255)
                                                                                                                                    aGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATtta             <  1:3912139/149‑1 (MQ=255)
                                                                                                                                      tttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttatt           <  2:3267449/149‑1 (MQ=255)
                                                                                                                                      tttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttatt           <  2:438628/149‑1 (MQ=255)
                                                                                                                                       ttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAAAAGAAATCCTGCTTATCCAACGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttatta          >  1:2497871/1‑149 (MQ=255)
                                                                                                                                            tataGAAGAAACCACTATCACGGAGTCGCTGGCAATTGATGTTGATGACGAGATAATGGAGTACGATGGTAGTTACTATAACAAGAAAGCCAGCTTCTCCAACGTTAAAAAATATAATAAAAAATGCAAAAATTAAAATTATTAaaata     >  1:1238431/1‑149 (MQ=255)
                                                                                                                                              taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaa   <  1:1635954/149‑1 (MQ=255)
                                                                                                                                               agaagaAACCACTATCACGGAGTCGCTGGCTATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaat  <  2:2370966/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAAT  >  CP009273/3996200‑3996491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑