BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,020,049	C→G	17.0%	P55A (CCG→GCG)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,020,049	0	C	G	17.0%	751.0 / 142.2	247	P55A (CCG→GCG)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base C (92/113); new base G (13/29); total (105/142)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 248 total aligned reads displayed.

CTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGA  >  CP009273/4019901‑4020194

  gTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCg                                                                                                                                                 >  2:347160/1‑149 (MQ=255)

   tAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGTTGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                                                                                                >  1:834346/1‑149 (MQ=255)

    aaaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGa                                                                                                                                               <  2:1807208/149‑1 (MQ=255)

     aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGAt                                                                                                                                              <  1:1907338/149‑1 (MQ=255)

     aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGAt                                                                                                                                              <  2:2300093/149‑1 (MQ=255)

     aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGAt                                                                                                                                              >  1:557727/1‑149 (MQ=255)

      aGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTAGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATg                                                                                                                                             >  1:396775/1‑149 (MQ=255)

       gTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGa                                                                                                                                            >  2:2471008/1‑149 (MQ=255)

            cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGCTGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAg                                                                                                                                       >  1:1473615/1‑149 (MQ=255)

            cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGTTGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAg                                                                                                                                       <  1:3044660/149‑1 (MQ=255)

               ggTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGt                                                                                                                                    <  2:1873794/149‑1 (MQ=255)

                gTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGtt                                                                                                                                   <  1:4075977/149‑1 (MQ=255)

                gTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCGGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGtt                                                                                                                                   >  1:2442110/1‑149 (MQ=255)

                    aaGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATc                                                                                                                               >  2:2160276/1‑149 (MQ=255)

                     aGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCg                                                                                                                              >  2:847638/1‑149 (MQ=255)

                     aGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCg                                                                                                                              >  2:404363/1‑149 (MQ=255)

                      gCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGa                                                                                                                             <  2:2082358/149‑1 (MQ=255)

                      gCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaaaa                                                                                                                                        >  1:3987979/1‑138 (MQ=255)

                      gCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGaaaaa                                                                                                                                        <  2:3987979/138‑1 (MQ=255)

                        tATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGc                                                                                                                           <  1:3718293/149‑1 (MQ=255)

                        tATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGc                                                                                                                           >  1:3343636/1‑149 (MQ=255)

                          tCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTg                                                                                                                         >  1:647339/1‑149 (MQ=255)

                             cGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCat                                                                                                                      <  2:1173448/149‑1 (MQ=255)

                              ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                                                                                     >  2:331489/1‑149 (MQ=255)

                               gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCatat                                                                                                                    <  1:833174/149‑1 (MQ=255)

                               gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCatat                                                                                                                    >  1:2740347/1‑149 (MQ=255)

                                   ccGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgc                                                                                                                <  2:3620566/149‑1 (MQ=255)

                                       atatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCtt                                                                                                            <  1:50302/149‑1 (MQ=255)

                                       atatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATGTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCtt                                                                                                            >  2:1072966/1‑149 (MQ=255)

                                        tctCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTGGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                                                                                           <  1:3135014/147‑1 (MQ=255)

                                         atCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCt                                                                                                          >  2:3910195/1‑149 (MQ=255)

                                          tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTg                                                                                                         >  1:1486780/1‑149 (MQ=255)

                                           cGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGa                                                                                                        <  2:2066889/149‑1 (MQ=255)

                                            gtgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGaa                                                                                                       <  1:437589/149‑1 (MQ=255)

                                             tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGaaa                                                                                                      <  2:3760028/149‑1 (MQ=255)

                                             tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGaaa                                                                                                      <  2:343055/149‑1 (MQ=255)

                                              gtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAt                                                                                                     <  2:3726198/149‑1 (MQ=255)

                                               tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATc                                                                                                    >  2:631062/1‑149 (MQ=255)

                                               tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATc                                                                                                    <  2:1609790/149‑1 (MQ=255)

                                                ccGCAGCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCa                                                                                                   <  2:1943306/149‑1 (MQ=255)

                                                 cGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCaa                                                                                                  <  2:488945/149‑1 (MQ=255)

                                                    aTCGTGCCAGACGCGGCCTTTTGTTTTCGTGGTGGTCAGTATTTGATGGTAGTGATGGATGATCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCGTCTGAAATCAACCt                                                                                               >  1:3783921/1‑149 (MQ=255)

                                                    aTCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCt                                                                                               <  1:342804/149‑1 (MQ=255)

                                                      cGTGCCAGACGCGGCCTTTTGTTTTCGTTCTGGTCATTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTAAAAGGCTTAGAAGCCGGAAGAAAAAAGGTTTATCGAGCTGCAAAATTGCGCTTCTGAAATAAAcaatt                                                                                             >  1:2158556/1‑145 (MQ=255)

                                                      cGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCttt                                                                                             <  1:2026142/149‑1 (MQ=255)

                                                       gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAGCCTTTa                                                                                            <  2:4078216/149‑1 (MQ=255)

                                                        tGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCGTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCATTAc                                                                                           >  1:3281326/1‑149 (MQ=255)

                                                        tGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGAAGTTAGTGATGGATTATCGCGACAAACCTCCGTTCTCAATGGCTTCGACGGCGGATAAAAAAGGTTTTATCTAGCTTCATATTTGCGCTTCTGAAaacaacaattaa                                                                                           >  1:1385073/1‑143 (MQ=255)

                                                          ccAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                                         <  1:936330/149‑1 (MQ=255)

                                                          ccAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                                         >  1:3603874/1‑149 (MQ=255)

                                                            aGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGa                                                                                       >  2:2044419/1‑149 (MQ=255)

                                                                 cgGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCa                                                                                  <  2:1530418/149‑1 (MQ=255)

                                                                  ggCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAg                                                                                 <  1:2709443/149‑1 (MQ=255)

                                                                       tttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATg                                                                            <  2:1640688/149‑1 (MQ=255)

                                                                          cttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAc                                                                         >  1:1002688/1‑149 (MQ=255)

                                                                                gTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATc                                                                   <  1:2926459/149‑1 (MQ=255)

                                                                                gTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATc                                                                   >  2:2947708/1‑149 (MQ=255)

                                                                                    tGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCa                                                               <  2:1330491/149‑1 (MQ=255)

                                                                                    tGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCa                                                               >  1:1266910/1‑149 (MQ=255)

                                                                                      gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCaaa                                                             <  1:2738162/149‑1 (MQ=255)

                                                                                      gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCaaa                                                             >  2:2353410/1‑149 (MQ=255)

                                                                                      gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCaaa                                                             <  2:2727043/149‑1 (MQ=255)

                                                                                      gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCaaa                                                             >  2:276321/1‑149 (MQ=255)

                                                                                             tttGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatcatc                                                      >  1:1131885/1‑149 (MQ=255)

                                                                                                gATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCaaa                                                   <  1:490904/149‑1 (MQ=255)

                                                                                                 aTGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAAt                                                  <  2:2338346/149‑1 (MQ=255)

                                                                                                   ggTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCg                                                >  1:1790273/1‑149 (MQ=255)

                                                                                                    gTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGt                                               <  2:1370753/149‑1 (MQ=255)

                                                                                                     tAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTg                                              >  2:2751240/1‑149 (MQ=255)

                                                                                                     tAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTg                                              <  2:1439393/149‑1 (MQ=255)

                                                                                                         gatggatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCg                                          >  1:2875295/1‑149 (MQ=255)

                                                                                                           tggatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGAc                                        <  1:1696241/149‑1 (MQ=255)

                                                                                                            ggatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACa                                       <  1:260982/149‑1 (MQ=255)

                                                                                                             gatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAt                                      <  2:3697008/149‑1 (MQ=255)

                                                                                                              atgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAtt                                     <  2:3017745/149‑1 (MQ=255)

                                                                                                                gAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcc                                   >  1:1895045/1‑149 (MQ=255)

                                                                                                                      gACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACgg                             <  2:1791000/149‑1 (MQ=255)

                                                                                                                      gACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCAGATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACgg                             >  1:2217962/1‑149 (MQ=255)

                                                                                                                       aCAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGTTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGga                            >  2:3235791/1‑149 (MQ=255)

                                                                                                                       aCAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGga                            <  2:2872461/149‑1 (MQ=255)

                                                                                                                       aCAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGga                            <  2:1267958/149‑1 (MQ=255)

                                                                                                                        cAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGgag                           <  1:361356/149‑1 (MQ=255)

                                                                                                                            cGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGc                       <  1:290714/149‑1 (MQ=255)

                                                                                                                             gTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCg                      >  1:3394041/1‑149 (MQ=255)

                                                                                                                             gTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCg                      <  1:3909461/149‑1 (MQ=255)

                                                                                                                              tCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGt                     <  1:714413/149‑1 (MQ=255)

                                                                                                                              tCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGt                     >  1:2534260/1‑149 (MQ=255)

                                                                                                                                 gTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCAACGGAGAAGCGTGGc                  >  1:3230826/1‑149 (MQ=255)

                                                                                                                                   tctcAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTg                >  2:4026174/1‑149 (MQ=255)

                                                                                                                                    ctcAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc               <  2:2834669/149‑1 (MQ=255)

                                                                                                                                    ctcAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc               <  2:773106/149‑1 (MQ=255)

                                                                                                                                    ctcAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc               <  1:3513745/149‑1 (MQ=255)

                                                                                                                                     tcAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgcg              >  1:1098812/1‑149 (MQ=255)

                                                                                                                                      cAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgcgc             <  1:2341303/149‑1 (MQ=255)

                                                                                                                                        aTGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCga           >  1:1180365/1‑149 (MQ=255)

                                                                                                                                         tGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCgat          <  1:2152309/149‑1 (MQ=255)

                                                                                                                                          ggCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCgatg         <  2:3941994/149‑1 (MQ=255)

                                                                                                                                              tCGACGCCTGATGAAAAAGGGTTTATCGAGCTGCATATTGGAGCTTCTGAAATCAACCTTTACGAGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCATATCGTTGTCGACATTCCCCACGGAGAATCGTGGCTGCGAGAtgatga     <  1:1183122/149‑1 (MQ=255)

                                                                                                                                                 aCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAaga  >  1:358822/1‑149 (MQ=255)

                                                                                                                                                 aCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAaga  <  1:1974360/149‑1 (MQ=255)

CTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGA  >  CP009273/4019901‑4020194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`