Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 332,599 G→T 100% A37A (GCG→GCT)  yahF → putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273332,5990GT98.0% 764.0 / 4.0 242A37A (GCG→GCT) yahFputative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (132/105);  total (134/108)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

GATGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTGAAG  >  CP009273/332452‑332744
                                                                                                                                                   |                                                                                                                                                 
 aTGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggctat                                                                                                                                                 <  1:1126006/149‑1 (MQ=255)
 aTGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggctat                                                                                                                                                 <  1:895775/149‑1 (MQ=255)
  tGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggctatg                                                                                                                                                <  2:3987563/149‑1 (MQ=255)
           gcTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGaa                                                                                                                                       >  2:2399457/1‑149 (MQ=255)
            cTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGaaa                                                                                                                                      <  1:752025/149‑1 (MQ=255)
                gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGaa                                                                                                                                  >  2:2938290/1‑149 (MQ=255)
                gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGaa                                                                                                                                  <  2:2664104/149‑1 (MQ=255)
                   aaaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaa                                                                                                                               >  1:4026318/1‑149 (MQ=255)
                   aaaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaa                                                                                                                               >  1:2480474/1‑149 (MQ=255)
                   aaaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaa                                                                                                                               <  2:1770405/149‑1 (MQ=255)
                    aaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaaa                                                                                                                              <  2:2290543/149‑1 (MQ=255)
                     aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAg                                                                                                                             >  1:2276573/1‑149 (MQ=255)
                     aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAg                                                                                                                             <  1:538769/149‑1 (MQ=255)
                      ccTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAgg                                                                                                                            <  2:1656202/149‑1 (MQ=255)
                          cGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTg                                                                                                                        >  2:3859811/1‑149 (MQ=255)
                           ggAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGc                                                                                                                       >  1:3637607/1‑149 (MQ=255)
                           ggAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGc                                                                                                                       <  2:458714/149‑1 (MQ=255)
                           ggAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGc                                                                                                                       >  1:3107874/1‑149 (MQ=255)
                           ggAGGGAATTATGTCAGTTAAAAGAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCGATCTCCACGCGTGCAACTAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGc                                                                                                                       <  2:3652109/149‑1 (MQ=255)
                             aGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAGATGAATAAAGGCGTGCTg                                                                                                                     >  1:309744/1‑149 (MQ=255)
                             aGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTg                                                                                                                     <  1:3671052/149‑1 (MQ=255)
                                 aaTTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTgaaga                                                                                                                 <  1:2147031/149‑1 (MQ=255)
                                 aaTTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTgaaga                                                                                                                 <  2:1482832/149‑1 (MQ=255)
                                 aaTTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAAGAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTgaaga                                                                                                                 <  2:3531828/149‑1 (MQ=255)
                                  aTTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGc                                                                                                                       >  1:222573/1‑142 (MQ=255)
                                  aTTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTgaagaa                                                                                                                >  2:2207700/1‑149 (MQ=255)
                                     aTGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATCAAGGCGTGCTGAAGAAttt                                                                                                             >  2:2465456/1‑149 (MQ=255)
                                      tGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTa                                                                                                            >  1:1922243/1‑149 (MQ=255)
                                       gTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAg                                                                                                           <  1:2271810/149‑1 (MQ=255)
                                        tCAGTTAAAATAGTCATTAAACCGAATACCTCTTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGACTACAGGCGTGCTGAAGAATTTCgg                                                                                                          >  2:2629479/1‑149 (MQ=255)
                                        tCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAgg                                                                                                          >  2:3254657/1‑149 (MQ=255)
                                         cAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGa                                                                                                         <  2:558973/149‑1 (MQ=255)
                                          aGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGAc                                                                                                        <  1:342850/149‑1 (MQ=255)
                                           gTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTTTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGAct                                                                                                       <  1:2947976/149‑1 (MQ=255)
                                           gTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGAct                                                                                                       >  1:3394302/1‑149 (MQ=255)
                                           gTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGAct                                                                                                       >  1:946390/1‑149 (MQ=255)
                                            ttAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGActg                                                                                                      >  2:105296/1‑149 (MQ=255)
                                              aaaaTAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGActgct                                                                                                    <  1:386494/149‑1 (MQ=255)
                                                aaTAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGa                                                                                                  <  2:2030658/149‑1 (MQ=255)
                                                  tAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACg                                                                                                >  1:682732/1‑149 (MQ=255)
                                                    gTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGcc                                                                                              >  1:485835/1‑149 (MQ=255)
                                                      cATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCgg                                                                                            >  2:252750/1‑149 (MQ=255)
                                                       aTTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGa                                                                                           >  2:180903/1‑149 (MQ=255)
                                                        ttAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAg                                                                                          >  1:2369735/1‑149 (MQ=255)
                                                            aCCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTgg                                                                                      >  1:96892/1‑149 (MQ=255)
                                                               gAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGc                                                                                   >  2:348595/1‑149 (MQ=255)
                                                                 aTACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAg                                                                                 <  1:2463227/149‑1 (MQ=255)
                                                                    ccTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCg                                                                              >  1:1234146/1‑149 (MQ=255)
                                                                     cTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGa                                                                             >  2:1988491/1‑149 (MQ=255)
                                                                      tATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaa                                                                            <  2:378385/149‑1 (MQ=255)
                                                                       aTTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGTAGGCGaaa                                                                           <  2:62148/149‑1 (MQ=255)
                                                                        ttttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAACTGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaa                                                                          >  2:1387846/1‑149 (MQ=255)
                                                                        ttttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaa                                                                          <  1:3971251/149‑1 (MQ=255)
                                                                        ttttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaa                                                                          >  2:1542935/1‑149 (MQ=255)
                                                                         tttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaaa                                                                         <  2:1018706/149‑1 (MQ=255)
                                                                         tttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaaa                                                                         <  2:3420848/149‑1 (MQ=255)
                                                                          ttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAAc                                                                        <  1:908430/149‑1 (MQ=255)
                                                                            gATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACgg                                                                      >  2:2988133/1‑149 (MQ=255)
                                                                                  gTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCt                                                                <  1:4075681/149‑1 (MQ=255)
                                                                                  gTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCt                                                                >  2:2523589/1‑149 (MQ=255)
                                                                                   tctcGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCtg                                                               >  1:1653957/1‑149 (MQ=255)
                                                                                     tcGCTGTTGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTAGGGCGACCGAAATTAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCtgat                                                             <  1:3196539/149‑1 (MQ=255)
                                                                                      cGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCtgatg                                                            <  2:805365/149‑1 (MQ=255)
                                                                                         tGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGAtt                                                         <  2:1653957/149‑1 (MQ=255)
                                                                                          gATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTg                                                        >  2:3271447/1‑149 (MQ=255)
                                                                                           aTGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGt                                                       >  2:3421945/1‑149 (MQ=255)
                                                                                           aTGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGt                                                       <  2:1330595/149‑1 (MQ=255)
                                                                                             gTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtca                                                     >  2:1218687/1‑149 (MQ=255)
                                                                                              tCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGAGTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtcat                                                    <  1:1732628/149‑1 (MQ=255)
                                                                                                 aTCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCaa                                                 >  1:2103747/1‑149 (MQ=255)
                                                                                                  tctcCACGGGTGCAAATAAACTCGACGGCGTCGCGCAGGCATTTGTGGATTTGGCGACCTAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAAt                                                <  1:2871060/149‑1 (MQ=255)
                                                                                                  tctcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAAt                                                <  2:2353347/149‑1 (MQ=255)
                                                                                                  tctcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAAt                                                >  1:2974019/1‑149 (MQ=255)
                                                                                                   ctcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATg                                               <  1:3244010/149‑1 (MQ=255)
                                                                                                       aCGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCGGGAGTTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaa                                           >  1:1310155/1‑149 (MQ=255)
                                                                                                       aCGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaa                                           >  2:2892298/1‑149 (MQ=255)
                                                                                                        cgcgTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaaa                                          >  2:2280090/1‑149 (MQ=255)
                                                                                                            tGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCgg                                      >  2:779275/1‑149 (MQ=255)
                                                                                                            tGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCgg                                      >  1:1078008/1‑149 (MQ=255)
                                                                                                                 agacaCTCGACGGCGTCGAGCAGGCATTTGGGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCg                                 <  2:741693/145‑1 (MQ=255)
                                                                                                                       tCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAAc                           >  1:2662892/1‑149 (MQ=255)
                                                                                                                          aCGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAg                        >  2:2712410/1‑149 (MQ=255)
                                                                                                                          aCGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAg                        >  2:3884338/1‑149 (MQ=255)
                                                                                                                              cGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGt                    <  2:710827/149‑1 (MQ=255)
                                                                                                                                tCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTa                  <  2:1347017/149‑1 (MQ=255)
                                                                                                                                   aGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtg               >  2:340057/1‑149 (MQ=255)
                                                                                                                                     cAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggt             >  2:1980763/1‑149 (MQ=255)
                                                                                                                                      aGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggtg            >  2:2932125/1‑149 (MQ=255)
                                                                                                                                      aGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggtg            <  1:1334113/149‑1 (MQ=255)
                                                                                                                                       ggCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggtgg           >  2:3099093/1‑149 (MQ=255)
                                                                                                                                       ggCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggtgg           >  2:1940874/1‑149 (MQ=255)
                                                                                                                                        gCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGga          >  1:3363204/1‑149 (MQ=255)
                                                                                                                                          aTTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGgaga        >  1:1407825/1‑149 (MQ=255)
                                                                                                                                           tttGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAt       >  2:4086252/1‑149 (MQ=255)
                                                                                                                                           tttGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAt       >  2:935440/1‑149 (MQ=255)
                                                                                                                                            ttGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAtt      >  1:2295980/1‑149 (MQ=255)
                                                                                                                                            ttGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAtt      <  2:1172215/149‑1 (MQ=255)
                                                                                                                                               tggctatggcgaCCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTgaa   >  1:1456762/1‑149 (MQ=255)
                                                                                                                                                ggctatggcgaCCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTgaag  >  1:2834033/1‑149 (MQ=255)
                                                                                                                                                ggctatggcgaCCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTgaag  >  1:771502/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GATGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTGAAG  >  CP009273/332452‑332744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑