Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 335,247 C→A 100% N404K (AAC→AAA)  yahG → DUF1116 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273335,2470CA97.8% 769.1 / 5.6 256N404K (AAC→AAA) yahGDUF1116 family protein
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (131/118);  total (135/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 256 total aligned reads displayed.

GACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGC  >  CP009273/335101‑335392
                                                                                                                                                  |                                                                                                                                                 
gACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGt                                                                                                                                                 <  2:3361732/149‑1 (MQ=255)
 aCAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTc                                                                                                                                                >  1:3214088/1‑149 (MQ=255)
 aCAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTc                                                                                                                                                >  1:2520477/1‑149 (MQ=255)
  cAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCa                                                                                                                                               <  2:1205175/149‑1 (MQ=255)
   aGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCAc                                                                                                                                              <  2:1692912/149‑1 (MQ=255)
    gcgcCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCAcc                                                                                                                                             <  1:2527293/149‑1 (MQ=255)
      gcCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCAt                                                                                                                                           >  2:47496/1‑149 (MQ=255)
              cGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgctgc                                                                                                                                   >  2:2952704/1‑149 (MQ=255)
               gAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgctgct                                                                                                                                  >  2:59151/1‑149 (MQ=255)
                     tACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGttt                                                                                                                            <  2:1098170/149‑1 (MQ=255)
                     tACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGttt                                                                                                                            <  2:411217/149‑1 (MQ=255)
                      aCGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGtttt                                                                                                                           <  2:2048361/149‑1 (MQ=255)
                      aCGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGtttt                                                                                                                           <  2:1251982/149‑1 (MQ=255)
                       cGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTa                                                                                                                          <  1:2814538/149‑1 (MQ=255)
                        ggTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTAt                                                                                                                         >  1:123551/1‑149 (MQ=255)
                         gTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATg                                                                                                                        >  2:1935604/1‑149 (MQ=255)
                         gTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATg                                                                                                                        <  1:3793303/149‑1 (MQ=255)
                          tATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATgg                                                                                                                       >  2:4099471/1‑149 (MQ=255)
                           aTTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATggg                                                                                                                      <  1:3053354/149‑1 (MQ=255)
                           aTTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATggg                                                                                                                      >  2:1042283/1‑149 (MQ=255)
                              ggCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGt                                                                                                                   >  2:3386762/1‑149 (MQ=255)
                              ggCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGt                                                                                                                   >  1:1149403/1‑149 (MQ=255)
                                 ggATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGcc                                                                                                                <  2:3299302/149‑1 (MQ=255)
                                  gATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCg                                                                                                               >  2:278611/1‑149 (MQ=255)
                                          aTGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAAt                                                                                                       <  1:2644710/149‑1 (MQ=255)
                                           tGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATc                                                                                                      <  2:2878285/149‑1 (MQ=255)
                                            ggCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCg                                                                                                     <  1:2785633/149‑1 (MQ=255)
                                            ggCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCg                                                                                                     >  2:3216191/1‑149 (MQ=255)
                                            ggCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCg                                                                                                     <  1:157470/149‑1 (MQ=255)
                                               gACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACa                                                                                                  <  1:2486164/149‑1 (MQ=255)
                                                  cgcgCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCa                                                                                               >  2:3122172/1‑149 (MQ=255)
                                                   gcgcCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCAc                                                                                              <  1:1054300/149‑1 (MQ=255)
                                                      cccGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCcg                                                                                           >  1:279052/1‑149 (MQ=255)
                                                        cGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAAGCGACATCACCcgcg                                                                                         >  2:1214649/1‑149 (MQ=255)
                                                          cTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTTGGCAATCGACATCACCCGCGTg                                                                                       >  1:3615717/1‑149 (MQ=255)
                                                          cTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTg                                                                                       >  1:3615508/1‑149 (MQ=255)
                                                          cTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTg                                                                                       <  1:1009306/149‑1 (MQ=255)
                                                           tATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTgg                                                                                      >  2:3069895/1‑149 (MQ=255)
                                                           tATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTgg                                                                                      >  2:1013939/1‑149 (MQ=255)
                                                            aTCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTggg                                                                                     <  2:1969586/149‑1 (MQ=255)
                                                            aTCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTggg                                                                                     <  2:4007438/149‑1 (MQ=255)
                                                            aTCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTggg                                                                                     <  1:3881919/149‑1 (MQ=255)
                                                             tcgtcgCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGt                                                                                    >  1:3963379/1‑149 (MQ=255)
                                                             tcgtcgCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGt                                                                                    <  2:2548293/149‑1 (MQ=255)
                                                                tcgCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTagc                                                                                 <  1:3409546/149‑1 (MQ=255)
                                                                       ggtggGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCa                                                                          <  1:476157/149‑1 (MQ=255)
                                                                         tggGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCAtt                                                                        <  2:2420134/149‑1 (MQ=255)
                                                                          gggCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTc                                                                       >  2:21342/1‑149 (MQ=255)
                                                                          gggCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTc                                                                       >  2:3484192/1‑149 (MQ=255)
                                                                          gggCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTc                                                                       >  2:3868935/1‑149 (MQ=255)
                                                                          gggCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTc                                                                       >  2:1432771/1‑149 (MQ=255)
                                                                          gggCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTc                                                                       >  1:3753294/1‑149 (MQ=255)
                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                   <  1:3676730/149‑1 (MQ=255)
                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                   <  2:666517/149‑1 (MQ=255)
                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                   <  2:605142/149‑1 (MQ=255)
                                                                                cACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCgg                                                                 <  1:3586183/149‑1 (MQ=255)
                                                                                  cGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTg                                                               <  2:3970030/149‑1 (MQ=255)
                                                                                  cGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTg                                                               <  2:3968919/149‑1 (MQ=255)
                                                                                  cGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTg                                                               >  1:3563725/1‑149 (MQ=255)
                                                                                    gTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGAt                                                             <  1:2099980/149‑1 (MQ=255)
                                                                                       gaagaaGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCaa                                                          >  1:980674/1‑149 (MQ=255)
                                                                                        aagaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAac                                                         >  2:866014/1‑149 (MQ=255)
                                                                                        aagaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAac                                                         <  2:570566/149‑1 (MQ=255)
                                                                                          gaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAacac                                                       >  1:2677792/1‑149 (MQ=255)
                                                                                           aagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACAcc                                                      >  1:2180307/1‑149 (MQ=255)
                                                                                            agCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCg                                                     <  1:2338334/149‑1 (MQ=255)
                                                                                             gCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGc                                                    <  1:160081/149‑1 (MQ=255)
                                                                                              cTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGcc                                                   >  1:2871269/1‑149 (MQ=255)
                                                                                              cTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGcc                                                   >  1:810118/1‑149 (MQ=255)
                                                                                                 ttGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATc                                                >  2:2206984/1‑149 (MQ=255)
                                                                                                 ttGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATc                                                <  2:2721204/149‑1 (MQ=255)
                                                                                                  tGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCg                                               >  2:3470089/1‑149 (MQ=255)
                                                                                                    aTTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTCAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGcc                                             >  1:606445/1‑149 (MQ=255)
                                                                                                     tttCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGccc                                            <  2:2411040/149‑1 (MQ=255)
                                                                                                        ctcCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATa                                         <  2:2674922/149‑1 (MQ=255)
                                                                                                        ctcCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATa                                         <  2:3688180/149‑1 (MQ=255)
                                                                                                        ctcCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATa                                         >  2:850803/1‑149 (MQ=255)
                                                                                                        ctcCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATa                                         <  2:1647994/149‑1 (MQ=255)
                                                                                                         tcCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATaa                                        >  1:1426265/1‑149 (MQ=255)
                                                                                                          cccGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATaaa                                       >  2:3005975/1‑149 (MQ=255)
                                                                                                              tCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATg                                   >  1:1793451/1‑149 (MQ=255)
                                                                                                                 gATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCgg                                >  1:3370102/1‑149 (MQ=255)
                                                                                                                        gAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATCAAGATGCGGGCGTCgg                         >  2:98131/1‑149 (MQ=255)
                                                                                                                        gAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCgg                         >  2:2175828/1‑149 (MQ=255)
                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCCTCGCCCATAAAGATGCGGGCGTCGGCa                       >  2:2390338/1‑149 (MQ=255)
                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCa                       >  2:156525/1‑149 (MQ=255)
                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCa                       <  1:198743/149‑1 (MQ=255)
                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCa                       >  2:2393156/1‑149 (MQ=255)
                                                                                                                                 cTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTgg                <  2:390250/149‑1 (MQ=255)
                                                                                                                                      tGAAAACCCCAACGTCACCAGTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCgg           <  2:1806853/149‑1 (MQ=255)
                                                                                                                                      tGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCgg           <  1:3868935/149‑1 (MQ=255)
                                                                                                                                      tGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCgg           <  2:1045713/149‑1 (MQ=255)
                                                                                                                                       gAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCggg          >  1:2917197/1‑149 (MQ=255)
                                                                                                                                        aaaaCCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGc         >  2:2596321/1‑149 (MQ=255)
                                                                                                                                          aaCCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCAt       <  1:4073923/149‑1 (MQ=255)
                                                                                                                                          aaCCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCAt       >  1:3102786/1‑149 (MQ=255)
                                                                                                                                            ccccAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCAGtg     >  2:2887134/1‑149 (MQ=255)
                                                                                                                                             cccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtgt    <  2:4024603/149‑1 (MQ=255)
                                                                                                                                             cccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtgt    <  1:445374/149‑1 (MQ=255)
                                                                                                                                               cAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGc  <  2:3446406/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGTGC  >  CP009273/335101‑335392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑