Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,135,168 G→T 100% V416L (GTA→TTA)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,135,1680GT97.3% 826.6 / 10.6 262V416L (GTA→TTA) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base T (96/159);  total (101/161)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCA  >  CP009273/4135022‑4135313
                                                                                                                                                  |                                                                                                                                                 
aCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTa                                                                                                                                                 >  2:1710232/1‑149 (MQ=255)
   ccTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTg                                                                                                                                              <  2:720424/149‑1 (MQ=255)
    cTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTgg                                                                                                                                             >  1:1514869/1‑149 (MQ=255)
     ttGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                            >  2:635581/1‑149 (MQ=255)
          cGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtg                                                                                                                                       >  1:633256/1‑149 (MQ=255)
              acGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGa                                                                                                                                   >  1:3010088/1‑149 (MQ=255)
                gCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                 >  1:1865973/1‑149 (MQ=255)
                gCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGGACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                 <  1:3190235/149‑1 (MQ=255)
                    ttCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATc                                                                                                                             >  2:393028/1‑149 (MQ=255)
                      ccTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTa                                                                                                                           <  2:88425/149‑1 (MQ=255)
                       cTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAt                                                                                                                          <  1:1967261/149‑1 (MQ=255)
                        tgatgaAAACCGCCGAAACCAGTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAtt                                                                                                                         <  2:2810864/149‑1 (MQ=255)
                          atgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                       >  1:2779897/1‑149 (MQ=255)
                          atgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                       <  1:3761913/149‑1 (MQ=255)
                           tgaAAACCGCCGAAACCATTCGCTTTGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTccc                                                                                                                      <  1:2613260/149‑1 (MQ=255)
                                aCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg                                                                                                                 <  1:1044733/149‑1 (MQ=255)
                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            >  1:366589/1‑149 (MQ=255)
                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            <  2:3263856/149‑1 (MQ=255)
                                     cgAAACCATTCGCGTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            <  2:2285804/149‑1 (MQ=255)
                                      gAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTa                                                                                                           <  1:2701579/149‑1 (MQ=255)
                                       aaaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                          <  1:3355024/149‑1 (MQ=255)
                                         aCCATTCGCTTCGGTACCGGTATTCCTCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGAGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACgg                                                                                                        >  1:1468426/1‑149 (MQ=255)
                                           cATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCt                                                                                                      <  2:2255293/149‑1 (MQ=255)
                                            cttCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCtt                                                                                                     <  2:463885/148‑1 (MQ=255)
                                            aTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCtt                                                                                                     >  2:3903369/1‑149 (MQ=255)
                                               cGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa                                                                                                  <  1:2027947/149‑1 (MQ=255)
                                                gcgtCGGGACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAGTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  2:1433472/146‑1 (MQ=255)
                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  1:2895549/149‑1 (MQ=255)
                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  2:211375/149‑1 (MQ=255)
                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 >  2:1207302/1‑149 (MQ=255)
                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg                                                                                                <  1:3923600/149‑1 (MQ=255)
                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGCGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg                                                                                                >  1:3475587/1‑149 (MQ=255)
                                                  ttCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                               <  1:2469651/149‑1 (MQ=255)
                                                  ttCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                               <  1:2350828/149‑1 (MQ=255)
                                                    cGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa                                                                                             <  2:2608064/149‑1 (MQ=255)
                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            <  1:560781/149‑1 (MQ=255)
                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            >  2:1813338/1‑149 (MQ=255)
                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            >  2:1994370/1‑149 (MQ=255)
                                                      gTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTCTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc                                                                                           >  1:3920549/1‑149 (MQ=255)
                                                        aCCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                                                                                         <  2:1647904/149‑1 (MQ=255)
                                                         ccGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                        >  2:2421758/1‑149 (MQ=255)
                                                          cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGATGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATGGCGa                                                                                       <  2:686443/149‑1 (MQ=255)
                                                          cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGa                                                                                       <  2:3627165/149‑1 (MQ=255)
                                                          cGGTAGTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGa                                                                                       <  1:3104860/149‑1 (MQ=255)
                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATg                                                                                     >  2:92389/1‑149 (MQ=255)
                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGTGCTGTGTGGAATTATCTATTACCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                    >  2:2925553/1‑149 (MQ=255)
                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                    <  2:1538827/149‑1 (MQ=255)
                                                              aTTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGtt                                                                                   <  1:3938386/149‑1 (MQ=255)
                                                              aTTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGtt                                                                                   <  1:3077099/149‑1 (MQ=255)
                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               <  2:1880170/149‑1 (MQ=255)
                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               >  1:2073065/1‑149 (MQ=255)
                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               <  1:3627053/149‑1 (MQ=255)
                                                                     aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                            >  1:3671246/1‑149 (MQ=255)
                                                                     aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                            >  2:130626/1‑149 (MQ=255)
                                                                         cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa                                                                        <  2:585060/149‑1 (MQ=255)
                                                                         cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa                                                                        <  1:175994/149‑1 (MQ=255)
                                                                          tttAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaa                                                                       <  1:1939809/149‑1 (MQ=255)
                                                                             aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGt                                                                    >  2:3724995/1‑149 (MQ=255)
                                                                             aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGt                                                                    >  2:3728410/1‑149 (MQ=255)
                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGa                                                                  <  2:443631/149‑1 (MQ=255)
                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGa                                                                  <  1:3879783/149‑1 (MQ=255)
                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  1:524205/149‑1 (MQ=255)
                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  2:1975962/149‑1 (MQ=255)
                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  1:3329029/149‑1 (MQ=255)
                                                                                  tGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATgg                                                               >  2:1760510/1‑149 (MQ=255)
                                                                                    aaGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaa                                                             <  1:824215/149‑1 (MQ=255)
                                                                                     aGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCCCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaaa                                                            <  2:961840/149‑1 (MQ=255)
                                                                                      gtggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           <  1:1314164/149‑1 (MQ=255)
                                                                                      gtggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           >  1:1250476/1‑149 (MQ=255)
                                                                                      gtggtggtgCCAGCGTTCCTCAACCGCGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           <  2:2564816/149‑1 (MQ=255)
                                                                                       tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATc                                                          <  2:160245/149‑1 (MQ=255)
                                                                                            gtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgcct                                                     <  1:1584531/149‑1 (MQ=255)
                                                                                              gCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGCCATCGCGATGTTTAATCTGCTGAAAGTGATGGCAATctgcctgc                                                   >  1:2148262/1‑149 (MQ=255)
                                                                                                cAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCAt                                                 <  1:960580/149‑1 (MQ=255)
                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  2:2486816/1‑149 (MQ=255)
                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  2:2339235/1‑149 (MQ=255)
                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  1:2251145/1‑149 (MQ=255)
                                                                                                    gTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGaaa                                             <  1:2863431/149‑1 (MQ=255)
                                                                                                        cTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                         >  1:484619/1‑149 (MQ=255)
                                                                                                             ccGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                    <  2:1179326/149‑1 (MQ=255)
                                                                                                             ccGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                    <  1:267393/149‑1 (MQ=255)
                                                                                                               gTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAt                                  >  2:3687240/1‑149 (MQ=255)
                                                                                                                   cGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctg                              <  1:4025309/149‑1 (MQ=255)
                                                                                                                    gTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctgc                             <  1:854295/149‑1 (MQ=255)
                                                                                                                      ttCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTTTCTCGTCACTTCATAACCTAGGGCTTGCATTACATCTCGATGTTTACTCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTgcgc                           <  1:3555522/149‑1 (MQ=255)
                                                                                                                            ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACtt                     >  1:1032318/1‑149 (MQ=255)
                                                                                                                            ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACtt                     <  1:2339235/149‑1 (MQ=255)
                                                                                                                                gATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGa                 >  2:3952054/1‑149 (MQ=255)
                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:2336004/1‑149 (MQ=255)
                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:3011268/1‑149 (MQ=255)
                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:1071394/1‑149 (MQ=255)
                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               <  2:2512426/149‑1 (MQ=255)
                                                                                                                                  tGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  1:4082283/1‑149 (MQ=255)
                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  1:3498702/1‑149 (MQ=255)
                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:313827/1‑149 (MQ=255)
                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:1387547/1‑149 (MQ=255)
                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGAGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:3046186/1‑149 (MQ=255)
                                                                                                                                            tATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGa     <  2:3475907/149‑1 (MQ=255)
                                                                                                                                             aTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAg    <  2:3323918/149‑1 (MQ=255)
                                                                                                                                              ttCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGc   >  2:2924292/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCA  >  CP009273/4135022‑4135313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑