Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,150,054 G→T 100% S222Y (TCT→TAT)  sthA ← pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,150,0540GT99.1% 717.5 / ‑2.1 220S222Y (TCT→TAT) sthApyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (119/98);  total (121/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

GACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149908‑4150199
                                                                                                                                                  |                                                                                                                                                 
gACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATaa                                                                                                                                                 <  1:2659090/149‑1 (MQ=255)
 aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAt                                                                                                                                                <  2:2461453/149‑1 (MQ=255)
 aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAt                                                                                                                                                >  2:1880020/1‑149 (MQ=255)
 aCCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAAt                                                                                                                                                >  1:2462877/1‑149 (MQ=255)
  ccGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATc                                                                                                                                               >  2:3040539/1‑149 (MQ=255)
  ccGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATc                                                                                                                                               >  1:1764908/1‑149 (MQ=255)
        gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                         <  2:696903/149‑1 (MQ=255)
        gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                         <  2:3852351/149‑1 (MQ=255)
        gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAt                                                                                                                                         <  2:2760690/149‑1 (MQ=255)
         cATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAtc                                                                                                                                        >  1:3565123/1‑149 (MQ=255)
          aTAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAtct                                                                                                                                       <  2:4028592/149‑1 (MQ=255)
          aTAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAtct                                                                                                                                       >  1:707258/1‑149 (MQ=255)
           tAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACAtctc                                                                                                                                      <  2:1545913/149‑1 (MQ=255)
             gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCtt                                                                                                                                    >  2:833871/1‑149 (MQ=255)
             gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCtt                                                                                                                                    <  1:2688646/149‑1 (MQ=255)
               tcagtcagtCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCAATACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  <  1:1077173/148‑1 (MQ=255)
               gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  <  1:2043323/149‑1 (MQ=255)
               gcaggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGa                                                                                                                                  <  1:418602/149‑1 (MQ=255)
                caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAt                                                                                                                                 <  2:3056537/149‑1 (MQ=255)
                caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGAt                                                                                                                                 >  1:673512/1‑149 (MQ=255)
                 aggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATc                                                                                                                                <  2:2719491/149‑1 (MQ=255)
                   gcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCga                                                                                                                              >  1:2955932/1‑149 (MQ=255)
                       tCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGaaa                                                                                                                          <  2:4106825/149‑1 (MQ=255)
                         aGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATg                                                                                                                        <  2:1801222/149‑1 (MQ=255)
                            tttCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATCCca                                                                                                                     >  2:2218754/1‑149 (MQ=255)
                              tCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagc                                                                                                                   >  1:2070140/1‑149 (MQ=255)
                               cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagca                                                                                                                  <  1:494680/149‑1 (MQ=255)
                               cAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagca                                                                                                                  >  1:2302993/1‑149 (MQ=255)
                                aGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCcagcag                                                                                                                 >  1:647860/1‑149 (MQ=255)
                                 gTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAgg                                                                                                                >  2:1831581/1‑149 (MQ=255)
                                      ttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATc                                                                                                           <  1:2488510/149‑1 (MQ=255)
                                         cccGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcgcg                                                                                                        <  2:3244295/149‑1 (MQ=255)
                                           cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCggg                                                                                                      >  2:3092014/1‑149 (MQ=255)
                                            gACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgt                                                                                                     <  2:272983/149‑1 (MQ=255)
                                             aCTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg                                                                                                    >  1:1097258/1‑149 (MQ=255)
                                              cTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtgt                                                                                                   <  2:2167204/149‑1 (MQ=255)
                                                 cAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGa                                                                                                >  1:3418066/1‑149 (MQ=255)
                                                 cAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGa                                                                                                <  2:2792142/149‑1 (MQ=255)
                                                  aGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGan                                                                                               <  1:3824635/149‑2 (MQ=255)
                                                  aGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAt                                                                                               >  1:793675/1‑149 (MQ=255)
                                                  aGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGAt                                                                                               <  1:2190407/149‑1 (MQ=255)
                                                   gATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATc                                                                                              >  1:1360448/1‑149 (MQ=255)
                                                       cATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGAt                                                                                          <  2:2151099/149‑1 (MQ=255)
                                                           aTCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCAc                                                                                      <  1:268497/149‑1 (MQ=255)
                                                             cacaCCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtt                                                                                    >  1:2318950/1‑149 (MQ=255)
                                                              acacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                   <  1:3393151/149‑1 (MQ=255)
                                                              acacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                   <  2:3399890/149‑1 (MQ=255)
                                                               cacCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtttt                                                                                  <  1:3635050/149‑1 (MQ=255)
                                                                     cGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATcc                                                                            <  1:1746939/149‑1 (MQ=255)
                                                                     cGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATcc                                                                            <  1:1110347/149‑1 (MQ=255)
                                                                      gTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCa                                                                           <  2:3673911/149‑1 (MQ=255)
                                                                       tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  2:3252438/1‑149 (MQ=255)
                                                                       tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  1:2273799/1‑149 (MQ=255)
                                                                       tCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCAt                                                                          >  1:2775316/1‑149 (MQ=255)
                                                                        cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa                                                                         <  2:372401/149‑1 (MQ=255)
                                                                          caGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAcc                                                                       >  1:927432/1‑149 (MQ=255)
                                                                           aGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACcg                                                                      >  2:1603901/1‑149 (MQ=255)
                                                                            gCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACcgc                                                                     >  1:1473407/1‑149 (MQ=255)
                                                                             ccTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACcgcg                                                                    <  1:2420561/149‑1 (MQ=255)
                                                                                tCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGaa                                                                 >  2:2491037/1‑149 (MQ=255)
                                                                                   aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              >  1:1253393/1‑149 (MQ=255)
                                                                                   aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              <  2:1022939/149‑1 (MQ=255)
                                                                                   aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              >  1:3482806/1‑149 (MQ=255)
                                                                                   aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              <  2:662925/149‑1 (MQ=255)
                                                                                   aTCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGAt                                                              <  1:1384394/149‑1 (MQ=255)
                                                                                    tcttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATc                                                             <  1:1966257/149‑1 (MQ=255)
                                                                                      ttctCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGa                                                           <  1:1124767/149‑1 (MQ=255)
                                                                                       tctcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGAc                                                          <  2:3619976/149‑1 (MQ=255)
                                                                                           gTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                      >  2:3503636/1‑149 (MQ=255)
                                                                                           gTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                      >  2:3425339/1‑149 (MQ=255)
                                                                                           gTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat                                                      >  1:907257/1‑149 (MQ=255)
                                                                                            tACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata                                                     >  1:3109003/1‑149 (MQ=255)
                                                                                             aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCatat                                                    >  1:2571950/1‑149 (MQ=255)
                                                                                             aCTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCatat                                                    >  2:284093/1‑149 (MQ=255)
                                                                                              ctctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATAtt                                                   <  1:3040539/149‑1 (MQ=255)
                                                                                               tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTc                                                  >  1:1768720/1‑149 (MQ=255)
                                                                                                    gTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc                                             <  1:3659704/149‑1 (MQ=255)
                                                                                                     ttGTGACGAATCACTACGCCACTGATCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc                                            >  1:2745347/1‑149 (MQ=255)
                                                                                                       gtgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGa                                          <  2:1510585/149‑1 (MQ=255)
                                                                                                       gtgACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGa                                          <  2:1139249/149‑1 (MQ=255)
                                                                                                               tCACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCa                                  <  2:2441967/149‑1 (MQ=255)
                                                                                                                cACTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAg                                 <  2:1777927/149‑1 (MQ=255)
                                                                                                                  cTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCa                               >  1:3935098/1‑149 (MQ=255)
                                                                                                                  cTACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCa                               <  2:2951798/149‑1 (MQ=255)
                                                                                                                   tACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              <  1:814762/149‑1 (MQ=255)
                                                                                                                   tACGCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                              >  1:2908708/1‑149 (MQ=255)
                                                                                                                      gCCACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCAt                           >  2:2029098/1‑149 (MQ=255)
                                                                                                                       ccACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATa                          >  1:3165907/1‑149 (MQ=255)
                                                                                                                       ccACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATa                          >  2:786772/1‑149 (MQ=255)
                                                                                                                        cACTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAg                         <  1:508561/149‑1 (MQ=255)
                                                                                                                          cTGTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGAt                       >  1:999223/1‑149 (MQ=255)
                                                                                                                            gTTCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATaa                     <  2:2363946/149‑1 (MQ=255)
                                                                                                                              tCCAGAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGt                   <  1:1666076/149‑1 (MQ=255)
                                                                                                                                  gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACAt               <  2:497046/149‑1 (MQ=255)
                                                                                                                                  gAAGTGATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACAt               <  2:154285/149‑1 (MQ=255)
                                                                                                                                       gATAGGAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgcgc          <  1:1185923/149‑1 (MQ=255)
                                                                                                                                           ggAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGtt      >  1:3596584/1‑149 (MQ=255)
                                                                                                                                           ggAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGtt      >  2:1108006/1‑149 (MQ=255)
                                                                                                                                           ggAGAGATAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGtt      >  1:1549688/1‑149 (MQ=255)
                                                                                                                                              gagataATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgt   <  1:4091071/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTG  >  CP009273/4149908‑4150199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑