Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,173,145 A→T 100% Q658L (CAG→CTG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,173,1450AT98.8% 1044.8 / 0.8 317Q658L (CAG→CTG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (156/157);  total (157/160)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 324 total aligned reads displayed.

CGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGT  >  CP009273/4172999‑4173290
                                                                                                                                                  |                                                                                                                                                 
cGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCctgc                                                                                                                                                 >  2:3839665/1‑149 (MQ=255)
    gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                             <  1:833426/149‑1 (MQ=255)
     aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtg                                                                                                                                            >  1:3253726/1‑149 (MQ=255)
        gCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTa                                                                                                                                         <  2:286510/149‑1 (MQ=255)
        gCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTa                                                                                                                                         <  1:3546067/149‑1 (MQ=255)
         cAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTAt                                                                                                                                        <  2:687105/149‑1 (MQ=255)
           aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATcc                                                                                                                                      >  1:3911272/1‑149 (MQ=255)
           aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATcc                                                                                                                                      >  2:1233673/1‑149 (MQ=255)
           aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATcc                                                                                                                                      <  2:3765645/149‑1 (MQ=255)
           aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATcc                                                                                                                                      <  2:3181389/149‑1 (MQ=255)
             tACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGt                                                                                                                                    >  1:3137555/1‑149 (MQ=255)
                gTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCgg                                                                                                                                 >  2:3870180/1‑149 (MQ=255)
                 ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGt                                                                                                                                >  2:3254139/1‑149 (MQ=255)
                  tATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTg                                                                                                                               >  1:1754141/1‑149 (MQ=255)
                  tATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTg                                                                                                                               <  2:1869744/149‑1 (MQ=255)
                   aTCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGc                                                                                                                              >  2:3820961/1‑149 (MQ=255)
                    tCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCACAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCg                                                                                                                             >  2:4021812/1‑149 (MQ=255)
                      gCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTc                                                                                                                           <  2:1584965/149‑1 (MQ=255)
                       cccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                                          <  2:1724718/149‑1 (MQ=255)
                            gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGAt                                                                                                                     <  1:2217721/149‑1 (MQ=255)
                                aCTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATccct                                                                                                                 >  1:3048656/1‑148 (MQ=255)
                                  tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCttt                                                                                                               <  1:2886580/149‑1 (MQ=255)
                                   ccAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTc                                                                                                              >  1:2070059/1‑149 (MQ=255)
                                    cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTcc                                                                                                             <  1:19998/149‑1 (MQ=255)
                                    cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTcc                                                                                                             <  2:1684243/149‑1 (MQ=255)
                                     aaCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCt                                                                                                            >  2:3139698/1‑149 (MQ=255)
                                      aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTg                                                                                                           <  2:2449779/149‑1 (MQ=255)
                                      aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTg                                                                                                           >  1:3514043/1‑149 (MQ=255)
                                          ggATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAac                                                                                                       <  1:661300/149‑1 (MQ=255)
                                          ggATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAac                                                                                                       <  2:621570/149‑1 (MQ=255)
                                           gATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                      <  1:3971960/149‑1 (MQ=255)
                                              gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGa                                                                                                   >  2:2746446/1‑149 (MQ=255)
                                               aagaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGAt                                                                                                  >  1:3450334/1‑149 (MQ=255)
                                                agaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATg                                                                                                 >  2:141875/1‑149 (MQ=255)
                                                 gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGa                                                                                                >  2:2600043/1‑149 (MQ=255)
                                                  aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGAc                                                                                               <  1:3332277/149‑1 (MQ=255)
                                                   agGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACg                                                                                              >  1:1314866/1‑149 (MQ=255)
                                                    ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGc                                                                                             >  1:2559431/1‑149 (MQ=255)
                                                     gCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGcc                                                                                            >  1:404945/1‑149 (MQ=255)
                                                        aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAAc                                                                                         <  1:2664646/149‑1 (MQ=255)
                                                        aCTACGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAAc                                                                                         >  2:3001878/1‑149 (MQ=255)
                                                          ttCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCg                                                                                       >  1:10386/1‑149 (MQ=255)
                                                           tCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGt                                                                                      <  2:2196455/149‑1 (MQ=255)
                                                            cGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTg                                                                                     <  1:72357/149‑1 (MQ=255)
                                                              tAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCa                                                                                   >  1:1458400/1‑149 (MQ=255)
                                                               agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAt                                                                                  <  2:2721793/149‑1 (MQ=255)
                                                                gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                 >  2:2024932/1‑149 (MQ=255)
                                                                gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                 >  2:3634834/1‑149 (MQ=255)
                                                                gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGGTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                 <  2:3702558/149‑1 (MQ=255)
                                                                  agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGcc                                                                                            >  2:2754345/1‑136 (MQ=255)
                                                                  agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                               <  1:766444/149‑1 (MQ=255)
                                                                   gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                              <  1:2484099/149‑1 (MQ=255)
                                                                     ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATgg                                                                            <  2:2309334/149‑1 (MQ=255)
                                                                        ggTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                         <  2:1122713/149‑1 (MQ=255)
                                                                         gTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCATCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                        <  2:578410/149‑1 (MQ=255)
                                                                             cTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGACCACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                                    >  2:2545263/1‑149 (MQ=255)
                                                                                gCCGTAGCACAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                 <  2:3921685/149‑1 (MQ=255)
                                                                                      gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                           <  2:2333525/149‑1 (MQ=255)
                                                                                       cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                          <  1:3673647/149‑1 (MQ=255)
                                                                                        aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                         >  2:961826/1‑149 (MQ=255)
                                                                                         aaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                        <  2:1201245/149‑1 (MQ=255)
                                                                                          aGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                       <  2:1241560/149‑1 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCACCGTCAGGc                                                      >  2:2429025/1‑149 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                      >  2:887176/1‑149 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                      >  2:2945567/1‑149 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                      >  1:1166401/1‑149 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                      <  2:2339601/149‑1 (MQ=255)
                                                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAGTGATGGGTGCGAACATGCAACGTCAGGc                                                      >  2:2407477/1‑149 (MQ=255)
                                                                                            gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGcc                                                     <  1:2380911/149‑1 (MQ=255)
                                                                                               aaTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGtt                                                  >  1:2733889/1‑149 (MQ=255)
                                                                                                aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                 <  1:1510677/149‑1 (MQ=255)
                                                                                                   cAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                              >  1:1666301/1‑149 (MQ=255)
                                                                                                   cAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                              >  1:2704608/1‑149 (MQ=255)
                                                                                                    aGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTTGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc                                             >  1:2336165/1‑149 (MQ=255)
                                                                                                       ttGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                          <  1:141875/149‑1 (MQ=255)
                                                                                                         gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGTGAACATGCAACGTCAGGCCGTTCCGACTCTgc                                        <  2:1388363/149‑1 (MQ=255)
                                                                                                         gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgc                                        >  1:4018278/1‑149 (MQ=255)
                                                                                                         gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgc                                        >  1:3829670/1‑149 (MQ=255)
                                                                                                             aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcgc                                    <  1:1135588/149‑1 (MQ=255)
                                                                                                              gCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCt                                   >  2:1503894/1‑149 (MQ=255)
                                                                                                              gCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCt                                   <  2:2058422/149‑1 (MQ=255)
                                                                                                                 gcgACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                                <  2:844842/149‑1 (MQ=255)
                                                                                                                  cgACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATa                               <  1:937048/149‑1 (MQ=255)
                                                                                                                      cAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGcc                           >  2:798034/1‑149 (MQ=255)
                                                                                                                        ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc                         >  2:3163398/1‑149 (MQ=255)
                                                                                                                        ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc                         >  1:3317980/1‑149 (MQ=255)
                                                                                                                        ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc                         >  1:3799802/1‑149 (MQ=255)
                                                                                                                        ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGc                         <  1:1233666/149‑1 (MQ=255)
                                                                                                                         gTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCGACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                        >  1:340308/1‑149 (MQ=255)
                                                                                                                         gTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                        <  1:1924193/149‑1 (MQ=255)
                                                                                                                          ttGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                       >  1:2809627/1‑149 (MQ=255)
                                                                                                                            gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                     <  2:2584345/149‑1 (MQ=255)
                                                                                                                               tACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                  <  1:1945513/149‑1 (MQ=255)
                                                                                                                                 cATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                <  2:1216977/149‑1 (MQ=255)
                                                                                                                                     gACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg            <  2:1924191/149‑1 (MQ=255)
                                                                                                                                     gACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg            <  2:1315079/149‑1 (MQ=255)
                                                                                                                                           tCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa      >  2:3388460/1‑149 (MQ=255)
                                                                                                                                            ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa     >  1:1839004/1‑149 (MQ=255)
                                                                                                                                               cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGt  >  2:223565/1‑149 (MQ=255)
                                                                                                                                               cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGt  >  1:609004/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGT  >  CP009273/4172999‑4173290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑