Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,210,753 C→A 100% E555* (GAA→TAA)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,210,7530CA98.7% 717.8 / ‑0.9 220E555* (GAA→TAA) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (114/102);  total (116/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

CGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGT  >  CP009273/4210607‑4210897
                                                                                                                                                  |                                                                                                                                                
    ttAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGc                                                                                                                                            >  1:1491164/1‑149 (MQ=255)
     gaaaCAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCt                                                                                                                                           <  2:1062174/148‑1 (MQ=255)
           gagTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTg                                                                                                                                     >  1:2555074/1‑149 (MQ=255)
              tGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGc                                                                                                                                  <  2:1561434/149‑1 (MQ=255)
                ggTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCaa                                                                                                                                <  1:3693138/149‑1 (MQ=255)
                 gTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAAt                                                                                                                               <  1:2684889/149‑1 (MQ=255)
                 gTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAAt                                                                                                                               >  2:2894804/1‑149 (MQ=255)
                  tAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATg                                                                                                                              >  1:2397097/1‑149 (MQ=255)
                   aaTGCTTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTAAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGc                                                                                                                             <  2:2520582/149‑1 (MQ=255)
                   aaTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGc                                                                                                                             <  2:726845/149‑1 (MQ=255)
                   aaTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGc                                                                                                                             <  1:616085/149‑1 (MQ=255)
                   aaTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGc                                                                                                                             <  2:2561676/149‑1 (MQ=255)
                    aTGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCt                                                                                                                            >  1:3118638/1‑149 (MQ=255)
                     tGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCtt                                                                                                                           <  1:617993/149‑1 (MQ=255)
                      gCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTc                                                                                                                          <  2:2170344/149‑1 (MQ=255)
                        gTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCaa                                                                                                                        <  1:125596/149‑1 (MQ=255)
                           aaaaaTATTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGaa                                                                                                                     <  2:2136491/149‑1 (MQ=255)
                           aaaaaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCTGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGaa                                                                                                                     >  1:2272566/1‑149 (MQ=255)
                           aaaaaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGaa                                                                                                                     <  2:2682774/149‑1 (MQ=255)
                            aaaaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAAt                                                                                                                    >  2:3359404/1‑149 (MQ=255)
                            aaaaTAGTATTAATAACGATCATGTGTCCGCGCTGCATGGCCATAAACAAAACCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGTCTAGTCTGTGCAATGCTTCAAGAAt                                                                                                                    >  2:4076558/1‑149 (MQ=255)
                              aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCCTGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATaa                                                                                                                  >  2:2371768/1‑149 (MQ=255)
                              aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATaa                                                                                                                  >  1:402766/1‑149 (MQ=255)
                              aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATaa                                                                                                                  >  1:405461/1‑149 (MQ=255)
                              aaTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATaa                                                                                                                  >  1:148327/1‑149 (MQ=255)
                               aTAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAAc                                                                                                                 >  2:776789/1‑149 (MQ=255)
                                 aGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACtt                                                                                                               >  1:1068361/1‑149 (MQ=255)
                                   tATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTc                                                                                                             <  2:154752/149‑1 (MQ=255)
                                   tATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTc                                                                                                             >  1:3273070/1‑149 (MQ=255)
                                     ttAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                           <  1:644526/149‑1 (MQ=255)
                                     ttAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                           <  2:1937919/149‑1 (MQ=255)
                                     ttAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATAGCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCAc                                                                                                           >  2:1215879/1‑149 (MQ=255)
                                      taataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACg                                                                                                          >  2:1088379/1‑149 (MQ=255)
                                       aataaCGTTCATGTGTCCGCGCTGCATGGCCATTAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAATCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGa                                                                                                         <  1:721176/149‑1 (MQ=255)
                                       aataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGa                                                                                                         <  1:285078/149‑1 (MQ=255)
                                        ataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGat                                                                                                        >  2:3682629/1‑149 (MQ=255)
                                        ataaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGat                                                                                                        >  1:1374585/1‑149 (MQ=255)
                                          aaCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGatat                                                                                                      >  1:2522044/1‑149 (MQ=255)
                                           aCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATc                                                                                                     >  2:2724145/1‑149 (MQ=255)
                                           aCGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATc                                                                                                     >  1:551653/1‑149 (MQ=255)
                                            cGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCg                                                                                                    >  1:2700180/1‑149 (MQ=255)
                                                   gtgtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTc                                                                                             <  1:1886307/149‑1 (MQ=255)
                                                    tgtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGACAATGCTTCAAGAATAACTTTCACGATATCGCTATGTcc                                                                                            <  2:1604981/149‑1 (MQ=255)
                                                     gtCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCa                                                                                           <  2:3567507/149‑1 (MQ=255)
                                                      tCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAt                                                                                          >  2:448122/1‑149 (MQ=255)
                                                        cgcgCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCAttt                                                                                        >  1:1629760/1‑149 (MQ=255)
                                                              gCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATc                                                                                  <  2:2458906/149‑1 (MQ=255)
                                                              gCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATc                                                                                  <  1:918371/149‑1 (MQ=255)
                                                              gCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATc                                                                                  >  2:3808605/1‑149 (MQ=255)
                                                                aTGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGc                                                                                <  2:707436/149‑1 (MQ=255)
                                                                       tAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGatat                                                                         <  1:3526986/149‑1 (MQ=255)
                                                                        aaacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGAtata                                                                        >  1:408107/1‑149 (MQ=255)
                                                                         aacaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAg                                                                       >  1:319428/1‑149 (MQ=255)
                                                                          acaaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGt                                                                      <  2:1713596/149‑1 (MQ=255)
                                                                            aaacCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                                                                    <  1:2943925/149‑1 (MQ=255)
                                                                               cccGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTgg                                                                 <  2:3160380/149‑1 (MQ=255)
                                                                                        cgcgcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATa                                                        <  2:402766/149‑1 (MQ=255)
                                                                                        cgcgcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATa                                                        <  2:1852381/149‑1 (MQ=255)
                                                                                        cgcgcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATa                                                        <  2:405461/149‑1 (MQ=255)
                                                                                        cgcgcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATa                                                        <  2:1325807/149‑1 (MQ=255)
                                                                                            cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATaaaaa                                                    >  1:1128632/1‑149 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  <  1:1574139/149‑1 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  >  1:1627620/1‑149 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  >  2:1276314/1‑149 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  >  1:1630614/1‑149 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  >  1:655462/1‑149 (MQ=255)
                                                                                              aGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc                                                  >  1:2717254/1‑149 (MQ=255)
                                                                                               gACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAATCATAAAAATcc                                                 >  1:1775117/1‑149 (MQ=255)
                                                                                                  ttttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCttt                                              <  2:2399524/149‑1 (MQ=255)
                                                                                                   tttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTg                                             <  1:1029323/149‑1 (MQ=255)
                                                                                                    ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGc                                            <  2:1369806/149‑1 (MQ=255)
                                                                                                     tAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                           <  1:1330380/149‑1 (MQ=255)
                                                                                                        cGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTc                                        <  1:787730/149‑1 (MQ=255)
                                                                                                         ggTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCa                                       <  1:3744198/149‑1 (MQ=255)
                                                                                                          gTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAg                                      >  2:1509822/1‑149 (MQ=255)
                                                                                                           tCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAgg                                     >  2:3311288/1‑149 (MQ=255)
                                                                                                             agaagaTCTACAATATCGGAAGCTGAAATGTTAATTTAGTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgag                                   >  1:2129718/1‑149 (MQ=255)
                                                                                                               aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAt                                 <  1:2862253/149‑1 (MQ=255)
                                                                                                               aagaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGAt                                 <  1:2073275/149‑1 (MQ=255)
                                                                                                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                >  2:2957390/1‑149 (MQ=255)
                                                                                                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                <  2:3059022/149‑1 (MQ=255)
                                                                                                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                <  1:2003494/149‑1 (MQ=255)
                                                                                                                   tCTACAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAg                             >  1:2285891/1‑149 (MQ=255)
                                                                                                                      aCAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAc                          >  1:1393660/1‑149 (MQ=255)
                                                                                                                      aCAATATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAc                          <  2:3522954/149‑1 (MQ=255)
                                                                                                                        aaTATCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCt                        >  2:690260/1‑149 (MQ=255)
                                                                                                                          tatCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGt                      >  1:3970525/1‑149 (MQ=255)
                                                                                                                            tCGGAAGCTGAAATGTTAATTTACTGGTCTAGGCTGTGCAATGATTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTc                    <  1:1657742/149‑1 (MQ=255)
                                                                                                                            tCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTc                    <  1:3702062/149‑1 (MQ=255)
                                                                                                                            tCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTc                    <  1:360406/149‑1 (MQ=255)
                                                                                                                            tCGGAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTc                    >  2:3871573/1‑149 (MQ=255)
                                                                                                                              ggAAGCTGAAATGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCACCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                  >  2:938834/1‑149 (MQ=255)
                                                                                                                                      aaaTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaaa          <  2:2104347/149‑1 (MQ=255)
                                                                                                                                      aaaTGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaaa          >  1:3218427/1‑149 (MQ=255)
                                                                                                                                         tGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGa       >  2:3331499/1‑149 (MQ=255)
                                                                                                                                         tGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGa       <  2:2254045/149‑1 (MQ=255)
                                                                                                                                         tGTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGa       <  2:178703/149‑1 (MQ=255)
                                                                                                                                          gTTAATTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc      >  1:3850966/1‑149 (MQ=255)
                                                                                                                                             aaTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtg   <  1:1152594/149‑1 (MQ=255)
                                                                                                                                              aTTTACTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtgt  >  2:3787605/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGT  >  CP009273/4210607‑4210897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑