Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,802 C→A 100% I662I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,8020CA98.2% 665.4 / 1.4 236I662I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (109/119);  total (112/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 236 total aligned reads displayed.

GACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTA  >  CP009273/32656‑32945
                                                                                                                                                  |                                                                                                                                               
gACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                               >  2:93323/1‑149 (MQ=255)
 acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGc                                                                                                                                              >  2:3679869/4‑149 (MQ=255)
    gCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAcc                                                                                                                                           >  2:3277555/1‑149 (MQ=255)
     ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCa                                                                                                                                          <  2:3475672/149‑1 (MQ=255)
      ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTCTAGGCCCCAg                                                                                                                                         >  1:2131014/1‑149 (MQ=255)
      ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                         >  1:664178/1‑149 (MQ=255)
       tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGc                                                                                                                                        >  2:1974617/1‑149 (MQ=255)
       tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGc                                                                                                                                        >  1:53922/1‑149 (MQ=255)
       tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGc                                                                                                                                        >  1:54200/1‑149 (MQ=255)
          aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCg                                                                                                                                     >  1:4026721/1‑149 (MQ=255)
            ttCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGa                                                                                                                                   >  1:3332491/1‑149 (MQ=255)
             tCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGAt                                                                                                                                  <  2:2755497/149‑1 (MQ=255)
               gAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                                >  2:1602082/1‑149 (MQ=255)
               gAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                                >  2:1522770/1‑149 (MQ=255)
               gAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                                >  1:391044/1‑149 (MQ=255)
                aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                               <  2:3351876/149‑1 (MQ=255)
                aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                               <  1:2654334/149‑1 (MQ=255)
                 gCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCAGAAAGGCGTTATCGTACAGTACGGCGGTCAGACCCAGCTGAAACTGGCGCGCGCGCTGGAATCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTa                                                                                                                              <  1:2704434/149‑1 (MQ=255)
                  ccGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTAt                                                                                                                             <  1:1237411/149‑1 (MQ=255)
                  ccGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTAt                                                                                                                             <  2:3264191/149‑1 (MQ=255)
                   cGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATc                                                                                                                            >  1:3594564/1‑149 (MQ=255)
                    ggTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCg                                                                                                                           >  1:1324232/1‑149 (MQ=255)
                       aaCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAcc                                                                                                                        <  1:900907/149‑1 (MQ=255)
                        aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCg                                                                                                                       <  1:2274617/149‑1 (MQ=255)
                          tctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAACGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATTGGCACCAGCCCGGATGCTATCGACCGTg                                                                                                                     <  1:1215014/149‑1 (MQ=255)
                          tctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTg                                                                                                                     <  1:278536/149‑1 (MQ=255)
                           ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGc                                                                                                                    <  1:2827731/149‑1 (MQ=255)
                            tGGAAGATGTGCTGGAAATCGTGCGTTTCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCGGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGct                                                                                                                   <  2:1935626/149‑2 (MQ=255)
                             ggAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCag                                                                                                                  >  1:3185313/1‑149 (MQ=255)
                              gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCaga                                                                                                                 >  2:2957042/1‑149 (MQ=255)
                               aaGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                >  1:2259667/1‑149 (MQ=255)
                                aGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaag                                                                                                               >  1:1186980/1‑149 (MQ=255)
                                  aTGTGCTGGAAATCGTGCGTATAGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAc                                                                                                             >  1:133163/1‑149 (MQ=255)
                                     tgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGt                                                                                                          <  2:2083793/149‑1 (MQ=255)
                                     tgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGt                                                                                                          <  1:3336446/149‑1 (MQ=255)
                                        tGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                       >  2:1660544/1‑149 (MQ=255)
                                        tGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                       >  1:3541324/1‑149 (MQ=255)
                                        tGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                       <  1:1497754/149‑1 (MQ=255)
                                           aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTCTCGACCGTGCAGAAGACCGTGAACGc                                                                                                    >  1:2901273/1‑149 (MQ=255)
                                           aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                    <  1:2525036/149‑1 (MQ=255)
                                           aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                    <  2:1027334/149‑1 (MQ=255)
                                            aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCt                                                                                                   <  2:1349667/149‑1 (MQ=255)
                                            aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCt                                                                                                   <  2:153943/149‑1 (MQ=255)
                                            aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCt                                                                                                   >  2:1990347/1‑149 (MQ=255)
                                             aTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGGGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTACAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCtt                                                                                                  <  1:2302476/149‑1 (MQ=255)
                                             aTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCtt                                                                                                  >  2:3248101/1‑149 (MQ=255)
                                              tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                                 <  1:1981001/149‑1 (MQ=255)
                                              tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                                 >  1:2360746/1‑149 (MQ=255)
                                                     tATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATg                                                                                          >  2:1421917/1‑149 (MQ=255)
                                                       tCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCg                                                                                        >  2:1786171/1‑149 (MQ=255)
                                                       tCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCg                                                                                        >  1:767603/1‑149 (MQ=255)
                                                       tCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCg                                                                                        >  2:3846325/1‑149 (MQ=255)
                                                         gagaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGt                                                                                      >  2:1849622/1‑149 (MQ=255)
                                                         gagaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGt                                                                                      >  2:4068532/1‑149 (MQ=255)
                                                          agaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                     >  1:3796526/1‑149 (MQ=255)
                                                          agaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                     >  1:3009779/1‑149 (MQ=255)
                                                          agaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGAACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                     <  1:2055555/149‑1 (MQ=255)
                                                           gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                    >  1:83301/1‑149 (MQ=255)
                                                              gCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGc                                                                                 <  1:1791604/149‑1 (MQ=255)
                                                                cGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGt                                                                               >  2:3965323/1‑149 (MQ=255)
                                                                cGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGt                                                                               <  1:3764518/149‑1 (MQ=255)
                                                                 gAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                              >  2:2727226/1‑149 (MQ=255)
                                                                    aGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctga                                                                           <  1:1536130/149‑1 (MQ=255)
                                                                      gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaa                                                                         >  1:736447/1‑149 (MQ=255)
                                                                         ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                      >  1:1007854/1‑149 (MQ=255)
                                                                         ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                      >  2:3591202/1‑149 (MQ=255)
                                                                               tCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                                <  2:389814/149‑1 (MQ=255)
                                                                                 cAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAAcc                                                              <  1:608293/149‑1 (MQ=255)
                                                                                  aGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCg                                                             <  1:3056364/149‑1 (MQ=255)
                                                                                  aGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCg                                                             <  1:2743081/149‑1 (MQ=255)
                                                                                    tACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGc                                                           <  2:3080680/149‑1 (MQ=255)
                                                                                    tACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACAGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGc                                                           <  2:844634/149‑1 (MQ=255)
                                                                                        gcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAAc                                                       <  1:3670193/149‑1 (MQ=255)
                                                                                           gTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGcc                                                    >  2:2787945/1‑149 (MQ=255)
                                                                                             cAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCAc                                                  >  1:2732950/1‑149 (MQ=255)
                                                                                              aGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCAcc                                                 >  2:2816291/1‑149 (MQ=255)
                                                                                               gACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCg                                                <  1:118330/149‑1 (MQ=255)
                                                                                               gACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCg                                                >  1:115753/1‑149 (MQ=255)
                                                                                                aCCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACCGAAACAACCGGCGAACGCCACCGt                                               <  2:186049/149‑1 (MQ=255)
                                                                                                 ccccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGtt                                              >  2:1035861/1‑149 (MQ=255)
                                                                                                  cccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTa                                             <  1:3304965/149‑1 (MQ=255)
                                                                                                      cTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGc                                         <  2:2353691/149‑1 (MQ=255)
                                                                                                      cTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGc                                         <  1:1962537/149‑1 (MQ=255)
                                                                                                         aaaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                      <  1:1714626/149‑1 (MQ=255)
                                                                                                         aaaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                      <  2:2777792/149‑1 (MQ=255)
                                                                                                         aaaCGGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGTATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                      <  1:81850/149‑1 (MQ=255)
                                                                                                          aaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAtt                                     >  1:2965642/1‑149 (MQ=255)
                                                                                                              ggCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTCTTGaaa                                 >  2:1539724/1‑149 (MQ=255)
                                                                                                                  cgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGc                             <  2:157922/149‑1 (MQ=255)
                                                                                                                        cTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTaga                       <  2:4001461/149‑1 (MQ=255)
                                                                                                                        cTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTaga                       <  2:3123766/149‑1 (MQ=255)
                                                                                                                         tGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTagag                      >  2:2491947/1‑149 (MQ=255)
                                                                                                                           gcaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa                    <  2:2597547/147‑1 (MQ=255)
                                                                                                                            aaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACCCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                   <  1:2222992/149‑1 (MQ=255)
                                                                                                                              gctgctGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGc                 <  2:1014655/149‑1 (MQ=255)
                                                                                                                                    ggCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAaga           >  2:1796524/1‑149 (MQ=255)
                                                                                                                                       gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt        <  2:1079364/149‑1 (MQ=255)
                                                                                                                                       gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt        >  2:1846332/1‑149 (MQ=255)
                                                                                                                                             gTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTa  >  2:3869126/1‑149 (MQ=255)
                                                                                                                                             gTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTa  >  2:3414372/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTA  >  CP009273/32656‑32945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑