BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,249,117	G→T	100%	intergenic (+68/‑48)	dinF → / → yjbJ	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,249,117	0	G	T	99.6%	776.1 / ‑4.2	235	intergenic (+68/‑48)	dinF/yjbJ	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (119/115); total (119/116)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAT  >  CP009273/4248973‑4249259

tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaaa                                                                                                                                            <  2:1711212/149‑1 (MQ=255)

   cGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTg                                                                                                                                         <  1:570488/149‑1 (MQ=255)

    gTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                        <  1:1541836/149‑1 (MQ=255)

    gTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                        <  1:1541029/149‑1 (MQ=255)

     ttGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGaa                                                                                                                                       <  2:3387061/149‑1 (MQ=255)

      tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAc                                                                                                                                      <  2:2989608/149‑1 (MQ=255)

      tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAc                                                                                                                                      >  1:1245748/1‑149 (MQ=255)

      tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAc                                                                                                                                      <  1:3078678/149‑1 (MQ=255)

         gcgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTa                                                                                                                                   <  2:124718/149‑1 (MQ=255)

         gcgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTa                                                                                                                                   <  1:690537/149‑1 (MQ=255)

         gcgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTa                                                                                                                                   <  2:2197413/149‑1 (MQ=255)

            ggCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTActc                                                                                                                                <  1:2023454/149‑1 (MQ=255)

            ggCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTActc                                                                                                                                >  2:2241193/1‑149 (MQ=255)

                   tctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTca                                                                                                                         >  1:1075377/1‑149 (MQ=255)

                       gcggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGACTAAAAGTGAACGTACTCTCATTCACAAc                                                                                                                     >  1:778228/1‑149 (MQ=255)

                        cggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAAcc                                                                                                                    <  1:168847/149‑1 (MQ=255)

                        cggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAAcc                                                                                                                    <  1:3742731/149‑1 (MQ=255)

                          gcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTa                                                                                                                  >  2:1362044/1‑149 (MQ=255)

                           cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTaa                                                                                                                 <  1:3467307/149‑1 (MQ=255)

                              tCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCAAAACCTAACGa                                                                                                              <  2:245777/149‑1 (MQ=255)

                                      cgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCt                                                                                                      <  1:2665668/149‑1 (MQ=255)

                                        tCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                    <  1:1010438/149‑1 (MQ=255)

                                          aCTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAt                                                                                                  >  1:90125/1‑149 (MQ=255)

                                              gcgcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATg                                                                                              >  1:1821006/1‑149 (MQ=255)

                                              gcgcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATg                                                                                              <  1:1763839/149‑1 (MQ=255)

                                                gcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGaa                                                                                            <  1:4086527/149‑1 (MQ=255)

                                                  aaTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATa                                                                                          >  1:1388869/1‑149 (MQ=255)

                                                     ggTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAg                                                                                       >  1:1086530/1‑149 (MQ=255)

                                                     ggTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAg                                                                                       >  1:1118801/1‑149 (MQ=255)

                                                      gTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGa                                                                                      >  2:1487495/1‑149 (MQ=255)

                                                       tACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTTAGCCAAATTGCTGACTACACTTAATCTCCCGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAt                                                                                     <  1:2304857/149‑1 (MQ=255)

                                                          cTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGaa                                                                                  >  1:3942468/1‑149 (MQ=255)

                                                           tGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAg                                                                                 >  1:2859436/1‑149 (MQ=255)

                                                            ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGc                                                                                >  1:140482/1‑149 (MQ=255)

                                                                 gCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcggcg                                                                           >  2:1890734/1‑149 (MQ=255)

                                                                    gCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                        <  2:9125/149‑1 (MQ=255)

                                                                       aCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACt                                                                     >  2:2784603/1‑149 (MQ=255)

                                                                       aCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACt                                                                     >  2:3654311/1‑149 (MQ=255)

                                                                          tGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGa                                                                  >  1:3369318/1‑149 (MQ=255)

                                                                          tGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGa                                                                  >  1:1190935/1‑149 (MQ=255)

                                                                          tGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGa                                                                  >  1:464514/1‑149 (MQ=255)

                                                                           gACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaa                                                                 <  1:2111707/149‑1 (MQ=255)

                                                                           gACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaa                                                                 >  2:500723/1‑149 (MQ=255)

                                                                            aCGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaaa                                                                <  2:110493/149‑1 (MQ=255)

                                                                             cGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAAc                                                               <  1:1362044/149‑1 (MQ=255)

                                                                              ggTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACa                                                              >  2:1717825/1‑149 (MQ=255)

                                                                              ggTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACa                                                              >  1:1834069/1‑149 (MQ=255)

                                                                               gTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                             <  1:2913398/149‑1 (MQ=255)

                                                                               gTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                             >  1:3661827/1‑149 (MQ=255)

                                                                                   aaaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTa                                                         <  2:1509967/149‑1 (MQ=255)

                                                                                   aaaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTa                                                         >  2:962416/1‑149 (MQ=255)

                                                                                    aaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaa                                                        <  1:986838/149‑1 (MQ=255)

                                                                                    aaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaa                                                        <  1:1968729/149‑1 (MQ=255)

                                                                                     aaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaaa                                                       <  1:799582/149‑1 (MQ=255)

                                                                                     aaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaaa                                                       >  1:2548110/1‑149 (MQ=255)

                                                                                     aaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCAAGTTCAGAATAAAAGTGAACGTACTCTCATTCACAAACTAACGATGAGGTCTTGATTATGAATAAAGATGACGACGGCGGTAACTGGAAACAGTTTaaa                                                       >  2:2651687/1‑149 (MQ=255)

                                                                                      aTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAg                                                      >  1:1325740/1‑149 (MQ=255)

                                                                                      aTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAg                                                      <  2:2071010/149‑1 (MQ=255)

                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGt                                                    <  2:1125769/149‑1 (MQ=255)

                                                                                         cTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTa                                                   <  2:3942468/149‑1 (MQ=255)

                                                                                           gAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaaa                                                 >  1:2802926/1‑149 (MQ=255)

                                                                                            aataaataATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAg                                                <  2:445835/149‑1 (MQ=255)

                                                                                             ataaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGt                                               >  2:3485842/1‑149 (MQ=255)

                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGGGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTg                                              >  1:1414514/1‑149 (MQ=255)

                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTg                                              >  2:2357109/1‑149 (MQ=255)

                                                                                                aataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaa                                            >  2:3658711/1‑149 (MQ=255)

                                                                                                 ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaaa                                           >  1:1170689/1‑149 (MQ=255)

                                                                                                  taatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAag                                          >  1:511973/1‑149 (MQ=255)

                                                                                                  taatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAag                                          <  2:3794354/149‑1 (MQ=255)

                                                                                                    atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                        <  2:2733341/149‑1 (MQ=255)

                                                                                                     tCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                       <  2:69516/149‑1 (MQ=255)

                                                                                                     tCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                       <  2:3079308/149‑1 (MQ=255)

                                                                                                       cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATTAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCaa                                     <  2:3300894/149‑1 (MQ=255)

                                                                                                       cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATTAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCaa                                     <  2:1300455/149‑1 (MQ=255)

                                                                                                       cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCac                                     >  2:647173/1‑148 (MQ=255)

                                                                                                       cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCaa                                     >  2:2493797/1‑149 (MQ=255)

                                                                                                       cTAAGCCAAAGTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTACAGGTAAAGTGAAAGAGCaa                                     >  2:1992121/1‑149 (MQ=255)

                                                                                                        tAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAAt                                    >  1:3256184/1‑149 (MQ=255)

                                                                                                         aaGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATg                                   >  1:2957246/1‑149 (MQ=255)

                                                                                                            ccAAATTGCTGACTACAATTAATCTCACTTTCATAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgggg                                <  1:905423/149‑1 (MQ=255)

                                                                                                            ccAAAGTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTAATCTCATTCACAACCTATCGATGAGGTCTTTATTATTAATATAGATGAAGCAGGCGGTTTCTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgggg                                <  1:2279050/149‑1 (MQ=255)

                                                                                                             cAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                               >  2:1845468/1‑149 (MQ=255)

                                                                                                             cAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                               <  2:3185626/149‑1 (MQ=255)

                                                                                                               aaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaa                             >  1:1501357/1‑149 (MQ=255)

                                                                                                                aTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaaa                            >  1:1914624/1‑149 (MQ=255)

                                                                                                                  tGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACt                          >  1:448283/1‑149 (MQ=255)

                                                                                                                     tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAc                       >  2:3013935/1‑149 (MQ=255)

                                                                                                                      gACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAcc                      >  2:1623357/1‑149 (MQ=255)

                                                                                                                        cTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCga                    >  2:738545/1‑149 (MQ=255)

                                                                                                                         tACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgat                   <  2:1117980/149‑1 (MQ=255)

                                                                                                                         tACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgat                   <  1:2778002/149‑1 (MQ=255)

                                                                                                                           cacTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatga                 >  2:3652536/1‑149 (MQ=255)

                                                                                                                             cTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgatg               >  2:583651/1‑149 (MQ=255)

                                                                                                                                aaTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata            <  2:87685/149‑1 (MQ=255)

                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGatat           >  2:3851338/1‑149 (MQ=255)

                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGatat           >  1:3678111/1‑149 (MQ=255)

                                                                                                                                  tctcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATg          <  2:3779472/149‑1 (MQ=255)

                                                                                                                                    tcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGAc        >  2:3547315/1‑149 (MQ=255)

                                                                                                                                      aCGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGa      <  2:3968755/149‑1 (MQ=255)

                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt  <  1:560006/149‑1 (MQ=255)

TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAT  >  CP009273/4248973‑4249259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`