Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 360,752 A→G 100% V331A (GTG→GCG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273360,7520AG97.4% 37397.8 / inf 9547V331A (GTG→GCG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (118/131);  new base G (4414/4873);  total (4534/5013)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 9551 total aligned reads displayed.

CTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360604‑360900
                                                                                                                                                    |                                                                                                                                                    
   gataagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCa                                                                                                                                                   <  1:4089021‑M1/104‑1 (MQ=255)
   gataagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTGCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCa                                                                                                                                                   <  1:461027‑M1/104‑1 (MQ=255)
     taagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAgg                                                                                                                                                 >  1:2239254‑M1/44‑149 (MQ=255)
       agctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggt                                                                                                                                               <  2:2743069‑M1/108‑1 (MQ=255)
         ctgtcaaaccagataaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtgg                                                                                                                                             <  2:2027872‑M1/110‑1 (MQ=255)
          tgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtggt                                                                                                                                            <  2:3027974‑M1/111‑1 (MQ=255)
           gtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtt                                                                                                                                           >  1:777420‑M1/38‑149 (MQ=255)
           gtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtt                                                                                                                                           <  2:3739435‑M1/112‑1 (MQ=255)
             caaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtttt                                                                                                                                         >  2:1452476‑M1/36‑149 (MQ=255)
               caccagatcacttcgcgctaacttacatgaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCTTGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGAGGTTTGCGTATTGGTCGCCAGGGTGGTttttc                                                                                                                                       >  2:86209‑M1/34‑149 (MQ=255)
               aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttc                                                                                                                                       >  1:194367‑M1/34‑149 (MQ=255)
                 ccagataaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctt                                                                                                                                     <  1:3463010‑M1/118‑1 (MQ=255)
                 caagccCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTttttctt                                                                                                                                     <  1:1227665/149‑1 (MQ=255)
                        aattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTCTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                              <  2:2391389‑M1/125‑1 (MQ=255)
                         attcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAg                                                                                                                             >  2:360752‑M1/24‑149 (MQ=255)
                         attcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAg                                                                                                                             <  1:216199‑M1/126‑1 (MQ=255)
                         attcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAg                                                                                                                             <  2:1145286‑M1/126‑1 (MQ=255)
                         attcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAg                                                                                                                             <  1:2391030‑M1/126‑1 (MQ=255)
                            cgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTga                                                                                                                          <  1:2323725‑M1/129‑1 (MQ=255)
                              cgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgaga                                                                                                                        >  1:543241‑M1/19‑149 (MQ=255)
                               gctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAc                                                                                                                       <  2:3319287‑M1/132‑1 (MQ=255)
                                   acttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAACAGTGAGACGGGc                                                                                                                   <  1:926307‑M1/136‑1 (MQ=255)
                                     ttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCaa                                                                                                                 >  1:3615207‑M1/12‑149 (MQ=255)
                                     ttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCaa                                                                                                                 <  2:2806864‑M1/138‑1 (MQ=255)
                                      tacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGTGGTTTTTGTTTTAAACAGTGGGACGGGGAAc                                                                                                                <  1:1796653‑M1/139‑1 (MQ=255)
                                         attaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTGTTTGTTTTCACCAGTGTGACGGGCAACAGc                                                                                                             <  1:1431227‑M1/142‑1 (MQ=255)
                                           taattgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAacagctgattgccct                                                                                                           >  1:3953691‑M1/6‑134 (MQ=255)
                                             attGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACCGCTGAt                                                                                                         <  2:3470664‑M1/146‑1 (MQ=255)
                                             attGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAt                                                                                                         >  1:1091871‑M1/4‑149 (MQ=255)
                                             attGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAt                                                                                                         >  2:624722‑M1/4‑149 (MQ=255)
                                              ttGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAtt                                                                                                        >  1:2870494‑M1/3‑149 (MQ=255)
                                                   ttGCGCTCACTGCCCGCTTTCCAGTCTGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCt                                                                                                   <  2:418557/149‑1 (MQ=255)
                                                       gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCCACAGCTGATTGCCCGTCAc                                                                                               >  1:2949441/1‑149 (MQ=255)
                                                       gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTGTTTCTTTTGAACACTGTGAAGGGCAAAACCAGATTGGCCTTCAc                                                                                               >  1:704915/1‑149 (MQ=255)
                                                        cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAcc                                                                                              <  2:3676091/149‑1 (MQ=255)
                                                             tGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAGTGAGACGGGCAACAGCTGATTTCCCATAAACGCCTg                                                                                         >  1:2474775/1‑149 (MQ=255)
                                                              gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTgg                                                                                        >  2:324054/1‑149 (MQ=255)
                                                                 cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGccc                                                                                     <  1:1723507/149‑1 (MQ=255)
                                                                 cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGccc                                                                                     <  2:3985955/149‑1 (MQ=255)
                                                                 cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCATCACCGCCTGGccc                                                                                     >  2:1765266/1‑149 (MQ=255)
                                                                    tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTga                                                                                  >  2:2252487/1‑149 (MQ=255)
                                                                       ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTGTTTTCACCACTGATAAGGGCAACACCAGATTTCCCATAACCCCCGGGCCCCgagag                                                                               >  1:1870526/1‑149 (MQ=255)
                                                                         aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGagagag                                                                             <  1:2767786/149‑1 (MQ=255)
                                                                          gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCCACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTTTTTTTACCCGTGAGAAGGGCAACAGCTGATTGCCCATCAACGCCAGGGCCGGAGAgtgt                                                                            >  1:3779111/1‑149 (MQ=255)
                                                                          gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGt                                                                            <  1:3235550/149‑1 (MQ=255)
                                                                          gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGt                                                                            <  1:3154680/149‑1 (MQ=255)
                                                                          gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGt                                                                            <  1:2009943/149‑1 (MQ=255)
                                                                            cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTGTTTTAAACAGTGAGACGGGCAACAGCTGATTTCCCATCACCCCCGGGCCCTGAGAGAGttt                                                                          >  1:3855743/1‑148 (MQ=255)
                                                                               gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcag                                                                       >  1:3313676/1‑149 (MQ=255)
                                                                                aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagc                                                                      <  2:3097419/149‑1 (MQ=255)
                                                                                 aaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagca                                                                     >  2:1827388/1‑149 (MQ=255)
                                                                                    cTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAGTTAGACGGGCCACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCaacc                                                                  >  1:2697317/1‑147 (MQ=255)
                                                                                      gTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCgg                                                                >  2:2456945/1‑149 (MQ=255)
                                                                                      gTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCgg                                                                <  2:1000065/149‑1 (MQ=255)
                                                                                          tGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCa                                                            >  2:266251/1‑149 (MQ=255)
                                                                                          tGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCa                                                            >  2:1910063/1‑149 (MQ=255)
                                                                                           gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGCGAGTTGCAGCAAGCGGTCCAc                                                           >  2:2592331/1‑149 (MQ=255)
                                                                                           gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAc                                                           <  1:3546672/149‑1 (MQ=255)
                                                                                              aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                        >  2:2314696/1‑149 (MQ=255)
                                                                                              aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                        <  2:1079843/149‑1 (MQ=255)
                                                                                               gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg                                                       >  2:1300434/1‑149 (MQ=255)
                                                                                               gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg                                                       >  2:563220/1‑149 (MQ=255)
                                                                                                cTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTgg                                                      <  2:3828536/149‑1 (MQ=255)
                                                                                                  gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGtt                                                    <  1:624413/149‑1 (MQ=255)
                                                                                                   cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                   <  2:1536916/149‑1 (MQ=255)
                                                                                                     ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGc                                                 >  2:2870391/1‑149 (MQ=255)
                                                                                                         tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGCGTGTTGCAGCACGCGGTCCACGCTGGTTTGCCCca                                             >  2:48887/1‑149 (MQ=255)
                                                                                                           aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATAGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGACCcagc                                           >  2:1683802/1‑149 (MQ=255)
                                                                                                                gCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCCACAGCTGATTGCCCGTAACCGCCGGGCCCTGAGAGAGTTGCAGCAATCGGTCCACGCTGGTTTGCCCCCGCAGGCg                                      >  1:2631184/1‑149 (MQ=255)
                                                                                                                gCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCg                                      <  1:1572540/149‑1 (MQ=255)
                                                                                                                   aaCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaaa                                   >  2:935889/1‑149 (MQ=255)
                                                                                                                   aaCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTAACCGCCTGGCCCGGAGAGAGTTGCAGCAAGCGGTCCACGCTGCTTTGACCCAGCAGGCGaaa                                   >  2:2519745/1‑149 (MQ=255)
                                                                                                                    aCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaaaa                                  <  1:1287904/149‑1 (MQ=255)
                                                                                                                    aCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaaaa                                  <  2:781080/149‑1 (MQ=255)
                                                                                                                      gcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATc                                >  2:1383061/1‑149 (MQ=255)
                                                                                                                       cgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATcc                               <  1:1161210/149‑1 (MQ=255)
                                                                                                                        gcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCt                              <  1:1000127/149‑1 (MQ=255)
                                                                                                                        gcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCt                              >  2:3907683/1‑149 (MQ=255)
                                                                                                                           gggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCTGCAGGCGAAAATCCTGtt                           <  1:1365381/149‑1 (MQ=255)
                                                                                                                            ggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGttt                          <  2:1609959/149‑1 (MQ=255)
                                                                                                                                 ggCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTTCCCCAGCAGGCGAAAATCCCGTTTGAtgg                     >  2:1233088/1‑149 (MQ=255)
                                                                                                                                 ggCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                     >  2:2939688/1‑149 (MQ=255)
                                                                                                                                   cGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtg                   <  1:2094351/149‑1 (MQ=255)
                                                                                                                                    ggTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                  >  2:2102912/1‑149 (MQ=255)
                                                                                                                                    ggTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                  <  2:4062706/149‑1 (MQ=255)
                                                                                                                                     gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                 <  1:307652/149‑1 (MQ=255)
                                                                                                                                      tttGCGTATTGGGCGCCAGGGTGGTTTTTATTTTAACCACAGAGACCGGCAACAGCTGATTGCCCAACACCGCCTGGCCCTGAGAGAGTTGCAGCAACCGGTACACGCTGGTTTTCCCCCGCAGGCGAAAATCCTGTTTGATTGTGGtt                >  2:714075/1‑149 (MQ=255)
                                                                                                                                           gTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg           >  2:317483/1‑149 (MQ=255)
                                                                                                                                           gTATTGGGCGCCAGGGTGGTTTTTCATTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg           >  2:3395585/1‑149 (MQ=255)
                                                                                                                                           gTATTGGGCGCCAGGGTGGTTTTTATTTTAACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACCCTGGTTTGCCCCAGCAGGCGAAAAACCTGTTTGATGGTGGTTAAcgg           >  1:2557419/1‑149 (MQ=255)
                                                                                                                                           gTATTGGGCACCAGGGTGGTTTTTATTTTCACCAGTGAGACGGGCAACACCTGATTTCCCGTCACCCCCGGGCCCGGAGAGAGTTTCAGCAAGCGGTCCACCCTGGTTTTCCCCCCCACGCGAAAAACCTGTTTGATGGTGGTTAAccg           >  1:841307/1‑147 (MQ=255)
                                                                                                                                            tATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc          >  2:1965391/1‑149 (MQ=255)
                                                                                                                                            tATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc          <  1:1501492/149‑1 (MQ=255)
                                                                                                                                             aTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcg         <  1:3705936/149‑1 (MQ=255)
                                                                                                                                             aTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcg         <  1:3390253/149‑1 (MQ=255)
                                                                                                                                              ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAACGGCAACAGCTGATTTCCCTTAACCGCCTGGCCCTGAGAGAGTTTCAGCAAGCGGTCCACGCTGGGTTTCCCCAGCAGGCGAAAATCATGTTTGATTGTGGTTAAcggcgg        >  1:1646167/1‑149 (MQ=255)
                                                                                                                                                gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa      <  2:3538255/149‑1 (MQ=255)
                                                                                                                                                gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa      >  1:3012166/1‑149 (MQ=255)
                                                                                                                                                gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCGGGCCCGGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa      >  1:4029653/1‑149 (MQ=255)
                                                                                                                                                 ggCGCCAGGGTGGATTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGat     <  2:2181386/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360604‑360900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑