Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,329,774 C→A 100% D189Y (GAC→TAC)  adiA ← arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,329,7740CA98.1% 659.5 / 1.5 232D189Y (GAC→TAC) adiAarginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (96/125);  total (101/131)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

GATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGG  >  CP009273/4329628‑4329918
                                                                                                                                                  |                                                                                                                                                
gatgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAgg                                                                                                                                                >  1:3352718/1‑149 (MQ=255)
 atgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGt                                                                                                                                               <  2:3666304/149‑1 (MQ=255)
  tgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTg                                                                                                                                              >  2:2164138/1‑149 (MQ=255)
   ggTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGc                                                                                                                                             <  1:66243/149‑1 (MQ=255)
   ggTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGc                                                                                                                                             <  1:3019882/149‑1 (MQ=255)
        ggTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAAc                                                                                                                                        >  1:2727268/1‑149 (MQ=255)
        ggTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAAc                                                                                                                                        >  1:4080518/1‑149 (MQ=255)
          ttAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAg                                                                                                                                      >  2:302589/1‑149 (MQ=255)
           tAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGa                                                                                                                                     <  2:3947918/149‑1 (MQ=255)
           tAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGa                                                                                                                                     <  2:1599615/149‑1 (MQ=255)
           tAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGa                                                                                                                                     <  1:927225/149‑1 (MQ=255)
             gagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCAGGTAGGTGCGGAACAGAtt                                                                                                                                   >  2:2649918/1‑149 (MQ=255)
              agCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGAttt                                                                                                                                  <  1:1831167/149‑1 (MQ=255)
                  ggAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCAc                                                                                                                              <  1:2801910/149‑1 (MQ=255)
                  ggAAGTACCGACGACTACCGACAAGCAGCGATCGCCACCAAATACGCGTGCGGCATATTTGTCGCTTTCGCCAAATGCTCCAGTAGGCTCAAGCACAGAAACGAGGGCAGTTCGTTCGATGACCATGTAGGTGCGGTACAGATTGTCaa                                                                                                                              >  2:2355753/1‑148 (MQ=255)
                    aaGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCa                                                                                                                            <  2:1809923/149‑1 (MQ=255)
                    aaGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCa                                                                                                                            <  2:2829430/149‑1 (MQ=255)
                     aGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAt                                                                                                                           <  1:646179/149‑1 (MQ=255)
                         ccGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagta                                                                                                                       <  2:1382362/149‑1 (MQ=255)
                          cgacgaCTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCTATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagtag                                                                                                                      >  2:1761154/1‑149 (MQ=255)
                            acgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTc                                                                                                                    <  1:1385586/149‑1 (MQ=255)
                            acgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTc                                                                                                                    <  2:639273/149‑1 (MQ=255)
                             cgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCa                                                                                                                   <  2:3182314/149‑1 (MQ=255)
                             cgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCa                                                                                                                   <  2:3887818/149‑1 (MQ=255)
                              gacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCAt                                                                                                                  <  2:2532669/149‑1 (MQ=255)
                                  aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTa                                                                                                              >  2:2455790/1‑149 (MQ=255)
                                   ccGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                             <  2:3832581/149‑1 (MQ=255)
                                   ccGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                             <  2:2598624/149‑1 (MQ=255)
                                     gACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCAGGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaa                                                                                                           >  1:3409271/1‑149 (MQ=255)
                                      aCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaaa                                                                                                          <  1:1408496/149‑1 (MQ=255)
                                          ggAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTc                                                                                                      >  2:3792179/1‑149 (MQ=255)
                                                 tCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCggg                                                                                               >  1:1724437/1‑149 (MQ=255)
                                                 tCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCggg                                                                                               >  1:3769726/1‑149 (MQ=255)
                                                  cGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCTGCGGgt                                                                                              <  2:1220381/149‑1 (MQ=255)
                                                  cGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGgt                                                                                              <  1:196167/149‑1 (MQ=255)
                                                   ggCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGgtg                                                                                             >  1:1437691/1‑149 (MQ=255)
                                                   ggCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGgtg                                                                                             >  1:47968/1‑149 (MQ=255)
                                                      aCCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                          >  1:1556074/1‑149 (MQ=255)
                                                       ccAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGtttt                                                                                         >  2:1528320/1‑149 (MQ=255)
                                                         aactccgcgTGCGGCATATTTTTCGCTTCCGCCAAATGCGCCCGTAGGGTCAAGCAAAGAACCGCGGGAAGTTCGTTCGATGCCCAGGTAGGTGCGGAACAGATTTTCACCATTGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTgg                                                                                       <  1:4008382/144‑1 (MQ=255)
                                                            tACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTCGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaa                                                                                    >  2:355352/1‑149 (MQ=255)
                                                            tACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaa                                                                                    >  2:3811747/1‑149 (MQ=255)
                                                                cgTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAAcc                                                                                <  1:2303181/149‑1 (MQ=255)
                                                                   gCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAc                                                                             <  2:650592/149‑1 (MQ=255)
                                                                      gCATATTTTTCGCTTTCGCCAAATGCGCCAGTAGGGTCACGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACAATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgcc                                                                          <  2:370424/149‑1 (MQ=255)
                                                                           tttttCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTCGAAACGACCGGCGGGTGGTTTGGTAAAACCAACGCCGCCCt                                                                     >  2:1275082/1‑149 (MQ=255)
                                                                            ttttCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtg                                                                    <  2:2225520/149‑1 (MQ=255)
                                                                             tttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtgg                                                                   >  1:2773073/1‑149 (MQ=255)
                                                                                   tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCt                                                             >  2:477534/1‑149 (MQ=255)
                                                                                   tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCt                                                             >  1:3662807/1‑149 (MQ=255)
                                                                                   tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCt                                                             >  1:207547/1‑149 (MQ=255)
                                                                                   tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCt                                                             >  1:3708695/1‑149 (MQ=255)
                                                                                     tcgcCAACTGCGCCAGTATGGTCACGCAAAGAACCGCGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACTGATTTTCACCTTAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTGGGTAAAACCAACGCCGCCCTGGTGGCCTgg                                                           <  2:1045913/149‑1 (MQ=255)
                                                                                     tcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTgg                                                           <  2:3439526/149‑1 (MQ=255)
                                                                                      cgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgc                                                          >  2:1479776/1‑149 (MQ=255)
                                                                                       gcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcg                                                         >  2:2328211/1‑149 (MQ=255)
                                                                                        ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                        >  2:188869/1‑149 (MQ=255)
                                                                                        ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGgcgc                                                        <  2:3134523/149‑1 (MQ=255)
                                                                                            aTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGcc                                                    <  1:3437096/149‑1 (MQ=255)
                                                                                              gcgcCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCa                                                  >  1:2948843/1‑149 (MQ=255)
                                                                                                  cAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGaa                                              <  2:540999/149‑1 (MQ=255)
                                                                                                   aGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACATATTTACACCATAGTAGTCAGGTTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAat                                             <  1:3536031/149‑1 (MQ=255)
                                                                                                   aGTAGGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGAGGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAat                                             <  1:2538726/149‑1 (MQ=255)
                                                                                                     tATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTACACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAatat                                           <  1:627522/149‑1 (MQ=255)
                                                                                                       tGGTCACGCAACGACCCGCGGGAAGTGCGTTCGAGGCCCATGTAGGTGCGGAACAGATTTTCCCCATCGTAGTCAGGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTc                                         <  1:3284500/149‑1 (MQ=255)
                                                                                                        ggTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCa                                        <  1:3073670/149‑1 (MQ=255)
                                                                                                         gtaaaGCAAAGAACCGTGGTATGTTCGTTCTATGCCCATGTAGGTGCGGAACTGATTTTCAACTTAGTAGTCATGGTAGAAACGTCCGGCGGTTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCAt                                       <  1:3437525/146‑1 (MQ=255)
                                                                                                          tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATg                                      <  1:1332641/149‑1 (MQ=255)
                                                                                                          tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATg                                      <  2:2714530/149‑1 (MQ=255)
                                                                                                            aaGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGa                                    >  2:2807655/1‑149 (MQ=255)
                                                                                                                aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                >  1:1794146/1‑149 (MQ=255)
                                                                                                                 aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               <  2:2292943/149‑1 (MQ=255)
                                                                                                                 aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               <  2:4080518/149‑1 (MQ=255)
                                                                                                                 aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               >  1:2448265/1‑149 (MQ=255)
                                                                                                                 aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                               <  2:2727268/149‑1 (MQ=255)
                                                                                                                   caaCCGCGGGAAGTGCGTTCTATGCCAATTTCGGTGCGCAACAGATTTTCACAATAGTATTCTTGTTATAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                             <  1:2420054/148‑1 (MQ=255)
                                                                                                                     aCCGAGGGAGGTTCTTTGGATGCCAATGTAGGGGGGGAACAGTTTTTCACATTTGTAGTCTTGGATTAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                           <  1:1960246/149‑1 (MQ=255)
                                                                                                                      ccGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtata                          <  2:2523309/149‑1 (MQ=255)
                                                                                                                       cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTatat                         <  2:1724448/149‑1 (MQ=255)
                                                                                                                        gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                        <  2:3409271/149‑1 (MQ=255)
                                                                                                                        gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                        <  1:1664827/149‑1 (MQ=255)
                                                                                                                        gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                        <  1:2925449/149‑1 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAttt                       >  2:1321131/1‑149 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAttt                       >  2:3796636/1‑149 (MQ=255)
                                                                                                                           ggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtca                     <  2:3259903/149‑1 (MQ=255)
                                                                                                                           ggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtca                     >  1:1398437/1‑149 (MQ=255)
                                                                                                                           ggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtca                     >  1:2559247/1‑149 (MQ=255)
                                                                                                                            gAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                    <  2:55820/149‑1 (MQ=255)
                                                                                                                                ttcgttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgc                <  2:887264/149‑1 (MQ=255)
                                                                                                                                  cgttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgc              >  2:3881528/1‑149 (MQ=255)
                                                                                                                                   gttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcg             >  2:3395278/1‑149 (MQ=255)
                                                                                                                                   gttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcg             >  2:2390156/1‑149 (MQ=255)
                                                                                                                                    ttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcgc            >  2:1834262/1‑149 (MQ=255)
                                                                                                                                     tcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCt           <  2:24790/149‑1 (MQ=255)
                                                                                                                                       gATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGa         >  1:2288965/1‑149 (MQ=255)
                                                                                                                                       gATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGa         >  1:792011/1‑149 (MQ=255)
                                                                                                                                           cccATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAg     >  2:2846365/1‑149 (MQ=255)
                                                                                                                                             cATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTg   <  2:2773073/149‑1 (MQ=255)
                                                                                                                                             cATGTAGGTGCGGAACAGATTGTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATAGTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTg   <  2:3196705/149‑1 (MQ=255)
                                                                                                                                              aTGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTgg  <  2:3942769/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGG  >  CP009273/4329628‑4329918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑