Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,358,920 G→T 100% R147L (CGC→CTC)  fxsA → suppressor of F exclusion of phage T7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,358,9200GT99.6% 740.0 / ‑4.4 226R147L (CGC→CTC) fxsAsuppressor of F exclusion of phage T7
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (91/134);  total (92/134)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 227 total aligned reads displayed.

ACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGC  >  CP009273/4358774‑4359064
                                                                                                                                                  |                                                                                                                                                
 cTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGa                                                                                                                                               <  1:2569467/149‑1 (MQ=255)
  ttttATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGAc                                                                                                                                              >  1:1009834/1‑149 (MQ=255)
  ttttATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGAc                                                                                                                                              <  1:1579045/149‑1 (MQ=255)
   tttATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGAcc                                                                                                                                             <  1:2440715/149‑1 (MQ=255)
       ttGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                         <  1:3918695/149‑1 (MQ=255)
       ttGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                         <  1:121740/149‑1 (MQ=255)
       ttGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                         >  2:3442796/1‑149 (MQ=255)
       ttGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCt                                                                                                                                         <  1:2197391/149‑1 (MQ=255)
        tGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCtt                                                                                                                                        <  2:1036162/149‑1 (MQ=255)
        tGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCtt                                                                                                                                        <  1:1795064/149‑1 (MQ=255)
        tGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCtt                                                                                                                                        <  1:1572437/149‑1 (MQ=255)
         gccgccGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTg                                                                                                                                       <  1:337866/149‑1 (MQ=255)
           cgccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGAt                                                                                                                                     <  1:1581632/149‑1 (MQ=255)
           cgccgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGAt                                                                                                                                     >  2:255188/1‑149 (MQ=255)
              cgGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCAt                                                                                                                                  <  2:4098750/149‑1 (MQ=255)
               ggTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATa                                                                                                                                 >  1:1622714/1‑149 (MQ=255)
               ggTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATa                                                                                                                                 >  1:3670081/1‑149 (MQ=255)
                   cAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGa                                                                                                                             <  2:4006393/149‑1 (MQ=255)
                    aaaaGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGAc                                                                                                                            <  1:2946458/149‑1 (MQ=255)
                       aGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGAt                                                                                                                         >  1:1281231/1‑149 (MQ=255)
                          aTCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGc                                                                                                                      >  1:3013328/1‑149 (MQ=255)
                           tCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGcc                                                                                                                     <  1:3156084/149‑1 (MQ=255)
                             tGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAg                                                                                                                   <  2:3471773/149‑1 (MQ=255)
                             tGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAg                                                                                                                   >  1:3195508/1‑149 (MQ=255)
                             tGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAg                                                                                                                   <  2:3470979/149‑1 (MQ=255)
                                cAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGAt                                                                                                                <  1:663959/149‑1 (MQ=255)
                                 aGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGAtt                                                                                                               <  2:2111289/149‑1 (MQ=255)
                                  gTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTa                                                                                                              >  2:1761204/1‑149 (MQ=255)
                                   tGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTaa                                                                                                             <  1:849539/149‑1 (MQ=255)
                                   tGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTaa                                                                                                             <  2:213037/149‑1 (MQ=255)
                                       gTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTc                                                                                                         >  1:3385173/1‑149 (MQ=255)
                                        ttGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCg                                                                                                        >  2:2761371/1‑149 (MQ=255)
                                          gATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGaa                                                                                                      >  2:336326/1‑149 (MQ=255)
                                                gCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCg                                                                                                >  2:2096000/1‑149 (MQ=255)
                                                    ttGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGAtt                                                                                            >  1:1080657/1‑149 (MQ=255)
                                                        gcTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTAtgt                                                                                        >  1:247673/1‑149 (MQ=255)
                                                         cTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTAtgtg                                                                                       >  2:215886/1‑149 (MQ=255)
                                                          ttttCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTgg                                                                                      >  1:397735/1‑149 (MQ=255)
                                                           tttCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGt                                                                                     <  1:305891/149‑1 (MQ=255)
                                                           tttCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGt                                                                                     >  1:3714020/1‑149 (MQ=255)
                                                             tctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTa                                                                                   <  2:490906/149‑1 (MQ=255)
                                                              ctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTAt                                                                                  <  2:2974229/149‑1 (MQ=255)
                                                              ctcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTAt                                                                                  >  2:3011237/1‑149 (MQ=255)
                                                               tcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTTATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATg                                                                                 >  1:1070889/1‑149 (MQ=255)
                                                               tcGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATg                                                                                 >  1:3949027/1‑149 (MQ=255)
                                                                 gCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGcc                                                                               <  2:3623892/149‑1 (MQ=255)
                                                                 gCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGcc                                                                               <  2:1577186/149‑1 (MQ=255)
                                                                     gCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCAttt                                                                           >  1:2055115/1‑149 (MQ=255)
                                                                        tGGCGGCGGTTTTCGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTcc                                                                        <  2:1080449/149‑1 (MQ=255)
                                                                         ggcggcGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCg                                                                       >  2:1705552/1‑149 (MQ=255)
                                                                         ggcggcGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCg                                                                       <  2:3286398/149‑1 (MQ=255)
                                                                               ggTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttt                                                                 >  2:785073/1‑149 (MQ=255)
                                                                                gTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                <  2:856985/149‑1 (MQ=255)
                                                                                gTTTTAGCGCCGGGACCGGGGGCGGTAATACTTTTGATGGTGAGTACCAGCTAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGtttt                                                                <  1:3595729/149‑1 (MQ=255)
                                                                                 ttttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGttttt                                                               <  2:542058/149‑1 (MQ=255)
                                                                                   ttAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCg                                                             >  1:2816633/1‑149 (MQ=255)
                                                                                     aGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGtt                                                           <  1:2689190/149‑1 (MQ=255)
                                                                                      gcgcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAGGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          <  2:1994333/149‑1 (MQ=255)
                                                                                      gcgcCGGGACCGGGGGCGTTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGCGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGttt                                                          <  1:1043038/149‑1 (MQ=255)
                                                                                        gcCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTg                                                        >  1:2640246/1‑149 (MQ=255)
                                                                                           gggACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGgca                                                     >  2:3110281/1‑149 (MQ=255)
                                                                                                 ggTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACa                                               <  1:2000375/149‑1 (MQ=255)
                                                                                                  gTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAg                                              >  1:1483201/1‑149 (MQ=255)
                                                                                                    ggCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGcc                                            >  2:1874842/1‑149 (MQ=255)
                                                                                                    ggCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGATTTGGCAGCAACAGcc                                            <  1:503548/149‑1 (MQ=255)
                                                                                                       ggTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATa                                         <  2:1281231/149‑1 (MQ=255)
                                                                                                       ggTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATa                                         <  1:3443512/149‑1 (MQ=255)
                                                                                                         tAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAc                                       >  1:2029571/1‑149 (MQ=255)
                                                                                                         tAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAc                                       >  2:340123/1‑149 (MQ=255)
                                                                                                          aaTACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAcc                                      >  2:1432062/1‑149 (MQ=255)
                                                                                                           aTACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAccc                                     <  1:3322874/149‑1 (MQ=255)
                                                                                                            tACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAAcccc                                    >  2:3864885/1‑149 (MQ=255)
                                                                                                             aCTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCg                                   <  1:3776304/149‑1 (MQ=255)
                                                                                                                tttGATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCa                                <  1:3287816/149‑1 (MQ=255)
                                                                                                                   gATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCa                             <  1:3556992/149‑1 (MQ=255)
                                                                                                                   gATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCa                             <  2:3736279/149‑1 (MQ=255)
                                                                                                                   gATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCa                             <  1:2364126/149‑1 (MQ=255)
                                                                                                                   gATGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCa                             <  2:1167529/149‑1 (MQ=255)
                                                                                                                    aTGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCAt                            <  2:1739703/149‑1 (MQ=255)
                                                                                                                     tGGTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATg                           <  2:1138465/149‑1 (MQ=255)
                                                                                                                      ggTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGa                          >  2:3940821/1‑149 (MQ=255)
                                                                                                                      ggTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGa                          <  2:31228/149‑1 (MQ=255)
                                                                                                                       gTGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGAt                         <  1:2034194/149‑1 (MQ=255)
                                                                                                                        tGAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATc                        <  2:161095/149‑1 (MQ=255)
                                                                                                                         gAGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCa                       >  2:1143370/1‑149 (MQ=255)
                                                                                                                          aGTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAg                      <  2:1352554/149‑1 (MQ=255)
                                                                                                                           gTACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAgc                     >  1:2640575/1‑149 (MQ=255)
                                                                                                                            tACCAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAgcg                    <  1:3236953/149‑1 (MQ=255)
                                                                                                                               cAGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCAt                 <  2:2627641/149‑1 (MQ=255)
                                                                                                                                aGCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATa                >  2:2438420/1‑149 (MQ=255)
                                                                                                                                 gCGAAAGGATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGTCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATag               >  1:3907246/1‑149 (MQ=255)
                                                                                                                                       ggATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACtt         <  1:1258449/149‑1 (MQ=255)
                                                                                                                                       ggATGATGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACtt         <  2:105249/149‑1 (MQ=255)
                                                                                                                                        gatgatGAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACttt        <  2:3337029/149‑1 (MQ=255)
                                                                                                                                         atgatgAGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACtttt       >  1:3561226/1‑149 (MQ=255)
                                                                                                                                           gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACttttac     >  1:1403169/1‑147 (MQ=255)
                                                                                                                                           gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTcc     <  2:1045484/149‑1 (MQ=255)
                                                                                                                                           gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTcc     >  2:1502983/1‑149 (MQ=255)
                                                                                                                                           gatgaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTcc     >  1:1130768/1‑149 (MQ=255)
                                                                                                                                              gaGCTCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGc  >  2:587692/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGGCGGTAATACTTTTGATGGTGAGTACCAGCGAAAGGATGATGAGCGCGACCGCCTTGATCATAAAGACGATCGCCAGGATTAATGTCGAAACGCCGGATTATGTGGTTATGCCATTTTCCGGCGTTTTTCGTTTTGGCAGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGC  >  CP009273/4358774‑4359064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑