Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,388,902 G→T 100% A558A (GCG→GCT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,388,9020GT100.0% 1010.3 / ‑6.7 310A558A (GCG→GCT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (171/138);  total (171/139)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 310 total aligned reads displayed.

GGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATC  >  CP009273/4388758‑4389047
                                                                                                                                                |                                                                                                                                                 
ggggATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGt                                                                                                                                               <  1:1042487/149‑1 (MQ=255)
 ggTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTg                                                                                                                                              <  1:142397/149‑1 (MQ=255)
  gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTgg                                                                                                                                             <  2:1537418/149‑1 (MQ=255)
  gTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTgg                                                                                                                                             >  2:1784393/1‑149 (MQ=255)
   tATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGa                                                                                                                                            >  1:3646670/1‑149 (MQ=255)
   tATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGa                                                                                                                                            <  2:3847601/149‑1 (MQ=255)
    aTTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAt                                                                                                                                           <  2:2834051/149‑1 (MQ=255)
    aTTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGAt                                                                                                                                           >  1:94047/1‑149 (MQ=255)
       gATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCGGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGc                                                                                                                                        <  2:1220228/149‑1 (MQ=255)
        aTTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCa                                                                                                                                       <  2:914120/149‑1 (MQ=255)
        aTTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCa                                                                                                                                       <  2:3312319/149‑1 (MQ=255)
         tttCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCAc                                                                                                                                      >  1:805791/1‑149 (MQ=255)
               gTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATc                                                                                                                                >  2:2152188/1‑149 (MQ=255)
                  aGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCACTATTGCTCAGTGGATTGCACGAAATCtga                                                                                                                             >  2:3441026/1‑149 (MQ=255)
                  aGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtga                                                                                                                             <  2:632760/149‑1 (MQ=255)
                   gATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtgat                                                                                                                            <  1:973323/149‑1 (MQ=255)
                    aTGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCtgatg                                                                                                                           <  2:2423865/149‑1 (MQ=255)
                      gCACAGCATGTTACCATCAGGGCAGTGCATAAACCCTAACGCCAACAAAATTTACAAATATTGATTCCTGAACTGATAGGCTACCTGGCGAATCAGTCCGTATTCTAACCTGGCAAAATTCCTCAGTGGATTGCACGAAATCTGATGAg                                                                                                                         >  2:722524/1‑149 (MQ=255)
                       cacaGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGc                                                                                                                        <  1:3026520/149‑1 (MQ=255)
                         caGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGa                                                                                                                      >  2:3230193/1‑149 (MQ=255)
                            cATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACa                                                                                                                   <  1:898232/149‑1 (MQ=255)
                             aTGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACAt                                                                                                                  <  1:3912553/149‑1 (MQ=255)
                                tgACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATgcg                                                                                                               >  1:2549297/1‑149 (MQ=255)
                                      tCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTgg                                                                                                         >  2:1309142/1‑149 (MQ=255)
                                      tCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTgg                                                                                                         >  2:3448481/1‑149 (MQ=255)
                                          ggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCaa                                                                                                     <  1:3788875/149‑1 (MQ=255)
                                          ggCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCaa                                                                                                     <  1:1619660/149‑1 (MQ=255)
                                             aGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATgg                                                                                                  >  2:2878665/1‑149 (MQ=255)
                                              gTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGc                                                                                                 <  2:1560776/149‑1 (MQ=255)
                                               tGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGca                                                                                                >  1:1510335/1‑149 (MQ=255)
                                                gCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcac                                                                                               <  1:3485552/149‑1 (MQ=255)
                                                gCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcac                                                                                               >  2:3902689/1‑149 (MQ=255)
                                                gCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGcac                                                                                               >  2:1195457/1‑149 (MQ=255)
                                                  cTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAg                                                                                             <  1:3579688/149‑1 (MQ=255)
                                                  cTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAg                                                                                             <  1:2144006/149‑1 (MQ=255)
                                                    ttACCCTTACTCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGc                                                                                           <  1:3566935/149‑1 (MQ=255)
                                                     tACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGcc                                                                                          >  2:399618/1‑149 (MQ=255)
                                                       cccTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGAGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCAt                                                                                        <  2:218375/149‑1 (MQ=255)
                                                         cTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATaa                                                                                      >  2:2285514/1‑149 (MQ=255)
                                                           tACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAAcc                                                                                    >  1:2017044/1‑149 (MQ=255)
                                                            aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAAccc                                                                                   <  2:1954737/149‑1 (MQ=255)
                                                            aCGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAAccc                                                                                   <  1:3875960/149‑1 (MQ=255)
                                                                caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgct                                                                               >  1:2158189/1‑149 (MQ=255)
                                                                caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgct                                                                               >  1:4101122/1‑149 (MQ=255)
                                                                caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACActgct                                                                               >  2:1869901/1‑149 (MQ=255)
                                                                 aacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCctgctg                                                                              >  1:3152311/1‑149 (MQ=255)
                                                                  acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTgg                                                                             >  1:2074312/1‑149 (MQ=255)
                                                                    aaaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCg                                                                           >  1:1697964/1‑149 (MQ=255)
                                                                     aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCgg                                                                          >  1:2507057/1‑149 (MQ=255)
                                                                      aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGa                                                                         >  1:1118781/1‑149 (MQ=255)
                                                                      aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGa                                                                         >  1:2260833/1‑149 (MQ=255)
                                                                       aTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTCTTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGAc                                                                        >  1:1868216/1‑149 (MQ=255)
                                                                        tttACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACg                                                                       <  1:3716378/149‑1 (MQ=255)
                                                                          tACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTg                                                                     <  1:2678504/149‑1 (MQ=255)
                                                                             aactCTTGATTCCTGACCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGaa                                                                  <  1:2378127/146‑1 (MQ=255)
                                                                             aaaTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGaa                                                                  <  2:3115904/149‑1 (MQ=255)
                                                                               aTCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACg                                                                >  1:3270152/1‑149 (MQ=255)
                                                                                tCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATCGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACgg                                                               >  1:649310/1‑149 (MQ=255)
                                                                                  ttGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGtt                                                             >  2:268644/1‑149 (MQ=255)
                                                                                     aTTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATg                                                          >  2:1420873/1‑149 (MQ=255)
                                                                                     aTTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATg                                                          <  1:2633165/149‑1 (MQ=255)
                                                                                      ttCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGt                                                         <  2:3725119/149‑1 (MQ=255)
                                                                                      ttCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGt                                                         >  1:1083874/1‑149 (MQ=255)
                                                                                       tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTc                                                        >  2:1669034/1‑149 (MQ=255)
                                                                                       tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTc                                                        <  2:2776619/149‑1 (MQ=255)
                                                                                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCaa                                                   >  1:1121614/1‑149 (MQ=255)
                                                                                            aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCaa                                                   <  1:1363411/149‑1 (MQ=255)
                                                                                             aCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAAc                                                  >  2:2982129/1‑149 (MQ=255)
                                                                                               tGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACtt                                                >  1:1799850/1‑149 (MQ=255)
                                                                                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                               >  1:1945402/1‑149 (MQ=255)
                                                                                                gATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTtg                                               <  1:2741397/149‑1 (MQ=255)
                                                                                                    ggCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaa                                           >  1:3314298/1‑149 (MQ=255)
                                                                                                      cTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGaaaa                                         <  2:469115/149‑1 (MQ=255)
                                                                                                       tACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAc                                        <  1:3198027/149‑1 (MQ=255)
                                                                                                         ccTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgc                                      <  2:2532134/149‑1 (MQ=255)
                                                                                                          cTGGCTAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc                                     >  1:983766/1‑149 (MQ=255)
                                                                                                            ggCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgccgc                                   >  2:3598/1‑149 (MQ=255)
                                                                                                               gAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCCCGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                <  2:1985955/149‑1 (MQ=255)
                                                                                                               gAAGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCgg                                <  2:94047/149‑1 (MQ=255)
                                                                                                                 aGCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggt                              >  2:3454117/1‑149 (MQ=255)
                                                                                                                  gCAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTGGTGAAAACGCCGCCGggtg                             <  2:203167/149‑1 (MQ=255)
                                                                                                                   cAGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtgg                            >  2:4069513/1‑149 (MQ=255)
                                                                                                                    aGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACTAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  1:1332890/1‑149 (MQ=255)
                                                                                                                    aGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  1:3469031/1‑149 (MQ=255)
                                                                                                                    aGTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGggtggt                           >  2:2355265/1‑149 (MQ=255)
                                                                                                                     gTCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTc                          <  2:2004723/149‑1 (MQ=255)
                                                                                                                      tCCGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCt                         >  1:4041849/1‑149 (MQ=255)
                                                                                                                        cGTATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGAGGTCTGt                       >  2:3510284/1‑149 (MQ=255)
                                                                                                                         gTATTCGACCCGGGCAATAGTGCTCAGTGGATTGCACTAAATCTGAGGTGCTAACAGGCGCTGTGGTCAATGGCACAGGCCATAACCCTGCGGGCGGACGTGGAAGGGTTATGGCCGCAACTGGTGAAAACGCCGCCGGGTGGTCTGtt                      <  1:590650/149‑1 (MQ=255)
                                                                                                                          tATTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACACTGCTGGCGGACGTGGACCGGTTATGTCCGCAACTTGTGAACACGCCGCCGGGTGGTCTGTTa                     >  1:2351307/1‑149 (MQ=255)
                                                                                                                           aTTCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTAc                    >  1:1580430/1‑149 (MQ=255)
                                                                                                                            ttCGAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACa                   >  1:2873072/1‑149 (MQ=255)
                                                                                                                               gAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATc                >  2:4022821/1‑149 (MQ=255)
                                                                                                                               gAACCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATc                <  2:548895/149‑1 (MQ=255)
                                                                                                                                aaCCTGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCACAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCt               >  1:2184892/1‑149 (MQ=255)
                                                                                                                                    tGGCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTg           >  2:882218/1‑149 (MQ=255)
                                                                                                                                      gCAATATTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAt         >  1:3991959/1‑149 (MQ=255)
                                                                                                                                         atatTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTa      <  2:2896075/149‑1 (MQ=255)
                                                                                                                                           atTGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa    <  2:1764920/149‑1 (MQ=255)
                                                                                                                                             tGCTCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc  <  1:3510204/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGGTATTGATTTCCAGTCAGATGCACAGCATGTGACCATCAGGGCAGTGCCTTTACCCTTACGCCAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATC  >  CP009273/4388758‑4389047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑