Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,394,875 G→T 100% A124A (GCG→GCT)  purA → adenylosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,394,8750GT98.6% 964.8 / 2.6 293A124A (GCG→GCT) purAadenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (137/152);  total (138/155)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 293 total aligned reads displayed.

TCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCC  >  CP009273/4394729‑4395017
                                                                                                                                                  |                                                                                                                                              
tctcCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTaa                                                                                                                                              >  1:3265609/1‑149 (MQ=255)
   ccGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGc                                                                                                                                           >  1:1377233/1‑149 (MQ=255)
      gCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGAt                                                                                                                                        <  1:2060767/149‑1 (MQ=255)
       ccGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATc                                                                                                                                       <  2:1457261/149‑1 (MQ=255)
       ccGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATc                                                                                                                                       <  1:224530/149‑1 (MQ=255)
            cTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGcac                                                                                                                                  >  1:2891617/1‑149 (MQ=255)
            cTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGcac                                                                                                                                  >  2:3680821/1‑149 (MQ=255)
              gatgaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGcacca                                                                                                                                >  2:1193280/1‑149 (MQ=255)
               atgaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGcaccac                                                                                                                               <  1:2740825/149‑1 (MQ=255)
               atgaaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGcaccac                                                                                                                               >  2:2001119/1‑149 (MQ=255)
                  aaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCgg                                                                                                                            >  2:1609501/1‑149 (MQ=255)
                  aaagagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCgg                                                                                                                            >  2:1988440/1‑149 (MQ=255)
                    agagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTc                                                                                                                          >  1:1807352/1‑149 (MQ=255)
                     gagatgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCg                                                                                                                         <  2:2183218/149‑1 (MQ=255)
                        atgaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTgg                                                                                                                      <  1:173923/149‑1 (MQ=255)
                          gaaagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTa                                                                                                                    >  1:1259694/1‑149 (MQ=255)
                            aagaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATc                                                                                                                  <  2:1883369/149‑1 (MQ=255)
                             agaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCg                                                                                                                 >  2:222553/1‑149 (MQ=255)
                              gaACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCgg                                                                                                                <  1:1662417/149‑1 (MQ=255)
                                 cTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGcc                                                                                                             <  1:3969262/149‑1 (MQ=255)
                                 cTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGcc                                                                                                             <  2:3269911/149‑1 (MQ=255)
                                    gAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGc                                                                                                          >  1:3531434/1‑149 (MQ=255)
                                        aCCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTAt                                                                                                      <  2:2473740/149‑1 (MQ=255)
                                         ccGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGGTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATg                                                                                                     <  2:927053/149‑1 (MQ=255)
                                          cGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGa                                                                                                    <  1:2805288/149‑1 (MQ=255)
                                             ggCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGa                                                                                                 <  2:1354174/149‑1 (MQ=255)
                                                    ccGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTa                                                                                          <  2:100468/149‑1 (MQ=255)
                                                       ttCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCa                                                                                       <  2:3055100/149‑1 (MQ=255)
                                                       ttCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCa                                                                                       >  2:2507390/1‑149 (MQ=255)
                                                        tCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCAc                                                                                      >  1:716490/1‑149 (MQ=255)
                                                           tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTc                                                                                   <  1:1724295/149‑1 (MQ=255)
                                                           tGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTc                                                                                   <  1:1918818/149‑1 (MQ=255)
                                                            gAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcg                                                                                  >  1:1386107/1‑149 (MQ=255)
                                                             aGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTcgc                                                                                 <  2:1434024/149‑1 (MQ=255)
                                                               cGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCgg                                                                               <  1:2010453/149‑1 (MQ=255)
                                                                   tgctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTg                                                                           >  2:2983116/1‑149 (MQ=255)
                                                                    gctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGc                                                                          >  1:358245/1‑149 (MQ=255)
                                                                     ctgctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCg                                                                         <  1:275535/149‑1 (MQ=255)
                                                                        ctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCTCTGGATAACGCTCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCgtgt                                                                      <  2:2079864/149‑1 (MQ=255)
                                                                        ctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCgtgt                                                                      <  2:2627559/149‑1 (MQ=255)
                                                                        ctgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCgtgt                                                                      <  1:2488354/149‑1 (MQ=255)
                                                                         tgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGtt                                                                     >  2:780829/1‑149 (MQ=255)
                                                                         tgTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGtt                                                                     <  2:1712986/149‑1 (MQ=255)
                                                                          gTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTg                                                                    <  2:376114/149‑1 (MQ=255)
                                                                           tCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTgg                                                                   <  1:182323/149‑1 (MQ=255)
                                                                           tCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTgg                                                                   >  2:703091/1‑149 (MQ=255)
                                                                           tCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTgg                                                                   <  2:1167859/149‑1 (MQ=255)
                                                                            cTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGc                                                                  <  2:1377227/149‑1 (MQ=255)
                                                                             tGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCg                                                                 >  2:3801776/1‑149 (MQ=255)
                                                                              gAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGa                                                                <  2:3631409/149‑1 (MQ=255)
                                                                                aGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGAcc                                                              >  2:3137272/1‑149 (MQ=255)
                                                                                aGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGAcc                                                              >  1:477688/1‑149 (MQ=255)
                                                                                 gCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCt                                                             <  1:764244/149‑1 (MQ=255)
                                                                                   aTGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCttt                                                           >  1:211751/1‑149 (MQ=255)
                                                                                     gTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTc                                                         >  1:6572/1‑149 (MQ=255)
                                                                                         gCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACa                                                     >  2:24498/1‑149 (MQ=255)
                                                                                          cTGATCCTTGATTATCACGTTGCGCTGTATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACaa                                                    >  1:2809436/1‑149 (MQ=255)
                                                                                           tGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACaaa                                                   >  2:464180/1‑149 (MQ=255)
                                                                                           tGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACaaa                                                   <  1:3786762/149‑1 (MQ=255)
                                                                                             aTCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGa                                                 <  2:2891617/149‑1 (MQ=255)
                                                                                               ccTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGaaa                                               <  2:3918841/149‑1 (MQ=255)
                                                                                                cTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAAc                                              >  1:1144445/1‑149 (MQ=255)
                                                                                                  tGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCt                                            <  2:4104585/149‑1 (MQ=255)
                                                                                                    aTTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAAcc                                             >  1:2512988/1‑146 (MQ=255)
                                                                                                    aTTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTc                                          <  2:736873/149‑1 (MQ=255)
                                                                                                     ttATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCg                                         <  2:1804463/149‑1 (MQ=255)
                                                                                                      tATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGc                                        >  2:546590/1‑149 (MQ=255)
                                                                                                       aTCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCt                                       <  1:1424339/149‑1 (MQ=255)
                                                                                                         cACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGa                                     <  1:586541/149‑1 (MQ=255)
                                                                                                         cACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGa                                     >  1:3804074/1‑149 (MQ=255)
                                                                                                            gTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGaaaa                                  <  2:3353798/149‑1 (MQ=255)
                                                                                                              tGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAAc                                <  2:4089665/149‑1 (MQ=255)
                                                                                                                  cTGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGaa                            <  1:699067/149‑1 (MQ=255)
                                                                                                                   tGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGaaa                           <  1:2677107/149‑1 (MQ=255)
                                                                                                                   tGGATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGaaa                           <  1:2746766/149‑1 (MQ=255)
                                                                                                                    ggATAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaag                          >  2:2501550/1‑149 (MQ=255)
                                                                                                                      aTAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaagaa                        <  1:1899091/149‑1 (MQ=255)
                                                                                                                      aTAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaagaa                        <  1:1609501/149‑1 (MQ=255)
                                                                                                                       tAACGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAaagaag                       <  2:3531434/149‑1 (MQ=255)
                                                                                                                        aaCGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGt                      <  1:2956512/149‑1 (MQ=255)
                                                                                                                        aaCGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGt                      <  1:3325286/149‑1 (MQ=255)
                                                                                                                         aCGCGCGTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTg                     <  2:2450879/149‑1 (MQ=255)
                                                                                                                             gcgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATgg                 <  1:2043383/149‑1 (MQ=255)
                                                                                                                              cgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGa                <  1:3205756/149‑1 (MQ=255)
                                                                                                                              cgTGAGAAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGa                <  1:1157913/149‑1 (MQ=255)
                                                                                                                                tGAGAAAGCGCGTTGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAat              >  1:4076811/1‑149 (MQ=255)
                                                                                                                                ggagaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGTAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAat              >  1:2402361/2‑149 (MQ=255)
                                                                                                                                 gagaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAata             <  2:1320409/149‑1 (MQ=255)
                                                                                                                                  agaAAGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAatat            <  2:3872274/149‑1 (MQ=255)
                                                                                                                                  agaAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAatat            <  1:3680821/149‑1 (MQ=255)
                                                                                                                                    aaaGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATca          <  1:1009526/149‑1 (MQ=255)
                                                                                                                                    aaaGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATca          <  2:2799767/149‑1 (MQ=255)
                                                                                                                                      aGCGCGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATcaca        >  1:3385900/1‑149 (MQ=255)
                                                                                                                                       gcgcGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACaa       >  1:2139072/1‑149 (MQ=255)
                                                                                                                                       gcgcGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACaa       >  1:2540957/1‑149 (MQ=255)
                                                                                                                                       gcgcGTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACaa       >  1:470761/1‑149 (MQ=255)
                                                                                                                                          cgTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACtt    <  1:3132401/149‑1 (MQ=255)
                                                                                                                                          cgTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACtt    <  2:1236013/149‑1 (MQ=255)
                                                                                                                                           gTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTc   <  2:2574639/149‑1 (MQ=255)
                                                                                                                                           gTGGCGCTAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTc   <  1:1344411/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCGTGAGAAAGCGCGTGGCGCGAAAGCGATCGGCACCACCGGTCGTGGTATCGGGCCTGCTTATGAAGATAAAGTAGCACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCC  >  CP009273/4394729‑4395017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑