Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,404,169 G→T 100% A26A (GCG→GCT)  aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,404,1690GT99.3% 883.3 / ‑2.2 267A26A (GCG→GCT) aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (150/115);  total (152/115)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

TCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGC  >  CP009273/4404023‑4404314
                                                                                                                                                  |                                                                                                                                                 
tctcTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGctct                                                                                                                                                 <  1:2567180/149‑1 (MQ=255)
tctcTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGctct                                                                                                                                                 >  1:4109776/1‑149 (MQ=255)
tctcTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGctct                                                                                                                                                 <  2:3627535/149‑1 (MQ=255)
nctctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGctct                                                                                                                                                 >  1:3354793/2‑149 (MQ=255)
       cTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGaa                                                                                                                                          <  1:1510239/149‑1 (MQ=255)
       cTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGaa                                                                                                                                          <  2:3988282/149‑1 (MQ=255)
         gTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGc                                                                                                                                        >  1:659846/1‑149 (MQ=255)
         gTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGc                                                                                                                                        >  1:1970963/1‑149 (MQ=255)
          tCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCg                                                                                                                                       >  2:3108322/1‑149 (MQ=255)
             tGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTa                                                                                                                                    >  2:2012205/1‑149 (MQ=255)
                aTCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAAcg                                                                                                                                 >  2:1504815/1‑149 (MQ=255)
                aTCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAAcg                                                                                                                                 >  2:44955/1‑149 (MQ=255)
                    aTGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTCACGCGTg                                                                                                                             >  1:2284154/1‑149 (MQ=255)
                    aTGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTg                                                                                                                             <  2:3431269/149‑1 (MQ=255)
                    aTGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACATATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTg                                                                                                                             >  1:2735377/1‑149 (MQ=255)
                      ggCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGaa                                                                                                                           >  2:2011767/1‑149 (MQ=255)
                        cAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAgg                                                                                                                         <  2:87637/149‑1 (MQ=255)
                         aGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGt                                                                                                                        <  1:2152137/149‑1 (MQ=255)
                             aCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTgctg                                                                                                                    <  1:2925473/149‑1 (MQ=255)
                               cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTgctggc                                                                                                                  <  2:3107742/149‑1 (MQ=255)
                                aTACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct                                                                                                                 >  1:227849/1‑149 (MQ=255)
                                 tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTgctggctg                                                                                                                >  1:3585419/1‑149 (MQ=255)
                                  aCTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGACGCGGTAACGCGTGAAGGTGctggctgg                                                                                                               >  2:3317669/1‑149 (MQ=255)
                                    tAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGa                                                                                                             >  2:2911469/1‑149 (MQ=255)
                                      aTGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCATCTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATa                                                                                                           <  1:3113498/149‑1 (MQ=255)
                                        ggTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGc                                                                                                         >  1:331720/1‑149 (MQ=255)
                                        ggTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGc                                                                                                         >  2:3695569/1‑149 (MQ=255)
                                         gTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCg                                                                                                        >  2:1436807/1‑149 (MQ=255)
                                          tGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGa                                                                                                       <  2:1220590/149‑1 (MQ=255)
                                                  aTTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg                                                                                               >  2:3604350/1‑149 (MQ=255)
                                                    tGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATAAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTCACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc                                                                                             >  2:1170099/1‑149 (MQ=255)
                                                      aTGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg                                                                                           <  2:2674971/149‑1 (MQ=255)
                                                       tGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt                                                                                          >  2:667938/1‑149 (MQ=255)
                                                         gagggagACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAt                                                                                        >  2:3957807/1‑149 (MQ=255)
                                                           gggagACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg                                                                                      >  1:1363069/1‑149 (MQ=255)
                                                           gggagACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg                                                                                      >  1:538237/1‑149 (MQ=255)
                                                           gggagACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg                                                                                      <  1:1015855/149‑1 (MQ=255)
                                                               gaCACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTca                                                                                  >  2:3771612/1‑149 (MQ=255)
                                                               gaCACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTca                                                                                  >  1:1129027/1‑149 (MQ=255)
                                                               gaCACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTca                                                                                  >  1:2298460/1‑149 (MQ=255)
                                                                           tGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAAc                                                                      >  2:3835903/1‑149 (MQ=255)
                                                                           tGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAAc                                                                      <  2:1616214/149‑1 (MQ=255)
                                                                            ggCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg                                                                     <  2:1046524/149‑1 (MQ=255)
                                                                            ggCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg                                                                     >  1:3808580/1‑149 (MQ=255)
                                                                              cAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGTTGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc                                                                   >  2:2065259/1‑149 (MQ=255)
                                                                              cAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc                                                                   <  2:929693/149‑1 (MQ=255)
                                                                              cAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc                                                                   >  1:1669817/1‑149 (MQ=255)
                                                                               aaaCTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt                                                                  <  2:2362960/149‑1 (MQ=255)
                                                                                aaCTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg                                                                 >  1:3886377/1‑149 (MQ=255)
                                                                                    cacaCCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATc                                                             >  2:3142419/1‑149 (MQ=255)
                                                                                      cacCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc                                                           >  1:1551161/1‑149 (MQ=255)
                                                                                          gTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa                                                       >  2:526759/1‑149 (MQ=255)
                                                                                           tttttAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa                                                      <  1:93174/149‑1 (MQ=255)
                                                                                           tttttAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa                                                      >  2:1096109/1‑149 (MQ=255)
                                                                                           tttttAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa                                                      >  2:1094757/1‑149 (MQ=255)
                                                                                            ttttAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTACCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAc                                                     >  2:1461238/1‑149 (MQ=255)
                                                                                               tAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg                                                  <  2:584185/149‑1 (MQ=255)
                                                                                                aaTCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTggg                                                 >  2:765650/1‑149 (MQ=255)
                                                                                                 aTCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg                                                <  1:3466780/149‑1 (MQ=255)
                                                                                                 aTCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg                                                <  1:2790813/149‑1 (MQ=255)
                                                                                                 aTCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg                                                <  2:712880/149‑1 (MQ=255)
                                                                                                        tataCCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg                                         >  2:1312953/1‑149 (MQ=255)
                                                                                                         ataCCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc                                        >  2:1792842/1‑149 (MQ=255)
                                                                                                          taCCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg                                       >  2:2160575/1‑149 (MQ=255)
                                                                                                            ccATTAAATAACATCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGAGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGaa                                     >  1:1472985/1‑149 (MQ=255)
                                                                                                             cATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAt                                    <  2:2047781/149‑1 (MQ=255)
                                                                                                             cATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAt                                    >  2:1694221/1‑149 (MQ=255)
                                                                                                             cATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAt                                    <  1:2786194/149‑1 (MQ=255)
                                                                                                              aTTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtg                                   <  1:1820087/149‑1 (MQ=255)
                                                                                                              aTTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtg                                   >  1:1437286/1‑149 (MQ=255)
                                                                                                              aTTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtg                                   >  1:863094/1‑149 (MQ=255)
                                                                                                                  aaTAACAGCAACTTCTACCTGTCTGAGGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCGGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa                               <  2:2996872/149‑1 (MQ=255)
                                                                                                                  aaTAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa                               >  2:3951394/1‑149 (MQ=255)
                                                                                                                  aaTAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa                               >  2:2179987/1‑149 (MQ=255)
                                                                                                                  aaTAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa                               <  2:3210697/149‑1 (MQ=255)
                                                                                                                    tAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc                             <  1:2721397/149‑1 (MQ=255)
                                                                                                                    tAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc                             >  1:2098899/1‑149 (MQ=255)
                                                                                                                    tAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc                             <  1:909464/149‑1 (MQ=255)
                                                                                                                    tAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc                             >  2:889382/1‑149 (MQ=255)
                                                                                                                    tAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc                             >  1:3349515/1‑149 (MQ=255)
                                                                                                                       cAGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGc                          <  2:2702529/149‑1 (MQ=255)
                                                                                                                        aGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGcc                         <  1:3396744/149‑1 (MQ=255)
                                                                                                                        aGCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGcc                         <  2:2598662/149‑1 (MQ=255)
                                                                                                                         gCAACTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCt                        >  1:3036498/1‑149 (MQ=255)
                                                                                                                            aCTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGaa                     <  2:1691167/149‑1 (MQ=255)
                                                                                                                            aCTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGACTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGaa                     >  2:626138/1‑149 (MQ=255)
                                                                                                                            aCTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGACTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGaa                     >  2:4052553/1‑149 (MQ=255)
                                                                                                                             cTTATACCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGTCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAat                    >  1:3287733/1‑149 (MQ=255)
                                                                                                                               tataCCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAatta                  >  2:1863216/1‑149 (MQ=255)
                                                                                                                                ataCCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAattat                 <  2:3354793/149‑1 (MQ=255)
                                                                                                                                 taCCTGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAattatt                >  1:3513849/1‑149 (MQ=255)
                                                                                                                                     tGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTgcgc            <  2:2253053/149‑1 (MQ=255)
                                                                                                                                     tGTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTgcgc            >  1:79222/1‑149 (MQ=255)
                                                                                                                                      gTCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCt           >  1:972060/1‑149 (MQ=255)
                                                                                                                                       tCTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTa          >  2:792339/1‑149 (MQ=255)
                                                                                                                                        cTGATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTAc         >  1:803702/1‑149 (MQ=255)
                                                                                                                                          gATGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGa       <  2:1784794/149‑1 (MQ=255)
                                                                                                                                           aTGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGAt      <  1:3628588/149‑1 (MQ=255)
                                                                                                                                           aTGGCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGAt      <  1:1272551/149‑1 (MQ=255)
                                                                                                                                             ggCGCTCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATgc    >  2:3667627/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGC  >  CP009273/4404023‑4404314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑