Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,457,499 C→A 100% R20S (CGT→AGT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,457,4990CA97.7% 819.2 / 8.1 259R20S (CGT→AGT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (140/113);  total (144/115)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 259 total aligned reads displayed.

TCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGA  >  CP009273/4457353‑4457644
                                                                                                                                                  |                                                                                                                                                 
tCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGt                                                                                                                                                 >  2:2750329/1‑149 (MQ=255)
  ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGa                                                                                                                                               >  1:647710/1‑149 (MQ=255)
  ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGa                                                                                                                                               <  1:2497886/149‑1 (MQ=255)
  ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGa                                                                                                                                               <  1:2305210/149‑1 (MQ=255)
  ccTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGa                                                                                                                                               <  1:2304651/149‑1 (MQ=255)
   cTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGAt                                                                                                                                              <  2:2697971/149‑1 (MQ=255)
      cTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCa                                                                                                                                           >  2:163918/1‑149 (MQ=255)
        cAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                         <  1:1131904/149‑1 (MQ=255)
        cAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                         <  1:4015432/149‑1 (MQ=255)
        cAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                         <  2:1911411/149‑1 (MQ=255)
        cAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCAtt                                                                                                                                         >  2:3820150/1‑149 (MQ=255)
         aGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTg                                                                                                                                        >  1:3472919/1‑149 (MQ=255)
             ttattaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTg                                                                                                                                    >  2:3363418/1‑149 (MQ=255)
             ttattaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTg                                                                                                                                    >  1:1005046/1‑149 (MQ=255)
               attaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGgc                                                                                                                                  >  2:2143134/1‑149 (MQ=255)
                   ccTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGgcgcgc                                                                                                                              <  1:1187761/149‑1 (MQ=255)
                    cTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCgca                                                                                                                             <  1:943324/149‑1 (MQ=255)
                    cTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCgca                                                                                                                             <  1:2999982/149‑1 (MQ=255)
                      tCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCgcagc                                                                                                                           <  2:3940209/149‑1 (MQ=255)
                              ggCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTg                                                                                                                   >  1:34361/1‑149 (MQ=255)
                               gCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGa                                                                                                                  >  2:1829171/1‑149 (MQ=255)
                                cTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGaa                                                                                                                 <  2:1952568/149‑1 (MQ=255)
                                 ttCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAt                                                                                                                >  1:2825554/1‑149 (MQ=255)
                                 ttCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAt                                                                                                                >  2:472199/1‑149 (MQ=255)
                                 ttCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAt                                                                                                                <  1:234965/149‑1 (MQ=255)
                                   cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATAc                                                                                                              <  1:70943/149‑1 (MQ=255)
                                   cGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATAc                                                                                                              <  2:375086/149‑1 (MQ=255)
                                    gCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACg                                                                                                             <  2:4107187/149‑1 (MQ=255)
                                     ccACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACCGTGAATACgg                                                                                                            >  2:1862133/1‑149 (MQ=255)
                                       aCGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACggtg                                                                                                          <  1:723202/149‑1 (MQ=255)
                                       aCGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACggtg                                                                                                          <  1:1882135/149‑1 (MQ=255)
                                          ccTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTc                                                                                                       <  2:1098032/149‑1 (MQ=255)
                                           cTGAAGACATGTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTcc                                                                                                      <  1:3865991/149‑1 (MQ=255)
                                              aaGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCccgc                                                                                                   >  1:1330262/1‑149 (MQ=255)
                                                  cATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTc                                                                                               >  1:2726810/1‑149 (MQ=255)
                                                    tttCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCt                                                                                             >  2:1551334/1‑149 (MQ=255)
                                                         gTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGt                                                                                        >  2:231529/1‑149 (MQ=255)
                                                           aCTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgc                                                                                      >  2:4057222/1‑149 (MQ=255)
                                                            cTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTgcg                                                                                     >  2:3423170/1‑149 (MQ=255)
                                                                ttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAtt                                                                                 <  2:1678995/149‑1 (MQ=255)
                                                                ttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAtt                                                                                 >  1:2004018/1‑149 (MQ=255)
                                                                 tCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                <  2:3502657/149‑1 (MQ=255)
                                                                 tCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTg                                                                                >  2:1561487/1‑149 (MQ=255)
                                                                   aGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGcc                                                                              <  1:3775957/149‑1 (MQ=255)
                                                                       aGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaa                                                                          <  1:398435/149‑1 (MQ=255)
                                                                        gTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaa                                                                         >  2:3145319/1‑149 (MQ=255)
                                                                         tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaaa                                                                        <  1:3363418/149‑1 (MQ=255)
                                                                         tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaaa                                                                        <  1:600513/149‑1 (MQ=255)
                                                                         tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGaaaa                                                                        <  1:3860776/149‑1 (MQ=255)
                                                                          gCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAAt                                                                       <  1:3131973/149‑1 (MQ=255)
                                                                           cGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATg                                                                      <  2:738658/149‑1 (MQ=255)
                                                                                ggACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGt                                                                 <  2:34367/149‑1 (MQ=255)
                                                                                 gACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTg                                                                >  2:655536/1‑149 (MQ=255)
                                                                                  aCTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGGGCCTGAAAATGGCGGTGa                                                               <  2:1005046/149‑1 (MQ=255)
                                                                                   cTCCTTATTTTTAAAGAAATTTTTACCAGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCTTAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATTCGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGAt                                                              <  2:22870/149‑1 (MQ=255)
                                                                                   cTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGAt                                                              <  1:1619204/149‑1 (MQ=255)
                                                                                     ccTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGc                                                            >  2:747380/1‑149 (MQ=255)
                                                                                      cctttGTTTAAACAAATTTTTACCCGGCTCCTTCGGCAATTACCGTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGcc                                                           <  2:2686623/145‑1 (MQ=255)
                                                                                      cTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGcc                                                           >  1:3296469/1‑149 (MQ=255)
                                                                                       ttATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGccc                                                          >  2:2790123/1‑149 (MQ=255)
                                                                                        tATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCg                                                         >  2:660584/1‑149 (MQ=255)
                                                                                         aTGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCga                                                        >  2:3296216/1‑149 (MQ=255)
                                                                                         aTGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCga                                                        >  2:2002198/1‑149 (MQ=255)
                                                                                          tGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaa                                                       >  1:2574981/1‑149 (MQ=255)
                                                                                          tGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaa                                                       >  1:2575149/1‑149 (MQ=255)
                                                                                          tGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCgaa                                                       <  2:1175732/149‑1 (MQ=255)
                                                                                           gTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaag                                                      >  2:2509092/1‑149 (MQ=255)
                                                                                             ttAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaagaa                                                    <  1:1803206/149‑1 (MQ=255)
                                                                                             ttAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCgaagaa                                                    <  2:3591910/149‑1 (MQ=255)
                                                                                                aaGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaagaagaa                                                 <  1:219994/149‑1 (MQ=255)
                                                                                                aaGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCgaagaagaa                                                 >  2:3545281/1‑149 (MQ=255)
                                                                                                  gAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAAtt                                               <  1:3413617/149‑1 (MQ=255)
                                                                                                     aTTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATtgtg                                            <  1:2143134/149‑1 (MQ=255)
                                                                                                       ttttACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGa                                          <  1:364783/149‑1 (MQ=255)
                                                                                                                gCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCg                                 >  1:3589408/1‑149 (MQ=255)
                                                                                                                gCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCg                                 >  1:3460822/1‑149 (MQ=255)
                                                                                                                gCTCATTCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCg                                 >  1:1407958/1‑149 (MQ=255)
                                                                                                                     ttCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                            >  2:2545993/1‑149 (MQ=255)
                                                                                                                     ttCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                            >  2:3082232/1‑149 (MQ=255)
                                                                                                                     ttCGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACAcc                            <  2:2575149/149‑1 (MQ=255)
                                                                                                                       cGCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCa                          >  1:856286/1‑149 (MQ=255)
                                                                                                                        gCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCAt                         >  1:3322943/1‑149 (MQ=255)
                                                                                                                        gCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCAt                         >  1:4085820/1‑149 (MQ=255)
                                                                                                                        gCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCAt                         >  2:1749315/1‑149 (MQ=255)
                                                                                                                        gCCATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCAt                         >  2:1703412/1‑149 (MQ=255)
                                                                                                                         ccATTTACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATc                        >  1:4080343/1‑149 (MQ=255)
                                                                                                                            tttACCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCgg                     >  2:1439030/1‑149 (MQ=255)
                                                                                                                               aCCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACTTTCGACACCCATCCGGAAg                  >  2:2890835/1‑149 (MQ=255)
                                                                                                                               aCCTTCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAg                  >  2:556196/1‑149 (MQ=255)
                                                                                                                                 ctgcccGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAggg                <  1:2446868/146‑1 (MQ=255)
                                                                                                                                  ttCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGt               <  2:1162083/149‑1 (MQ=255)
                                                                                                                                   tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGtt              <  1:2708870/149‑1 (MQ=255)
                                                                                                                                   tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGtt              <  1:163918/149‑1 (MQ=255)
                                                                                                                                   tCCCGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGtt              <  1:470608/149‑1 (MQ=255)
                                                                                                                                    cccGTCTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTa             >  1:1230513/1‑149 (MQ=255)
                                                                                                                                         cTGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCa        <  2:856286/149‑1 (MQ=255)
                                                                                                                                          tGGTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAg       >  2:3314083/1‑149 (MQ=255)
                                                                                                                                           ggTTCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCAGTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAgg      <  1:3524792/149‑1 (MQ=255)
                                                                                                                                              tCATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCgg   >  2:1135425/1‑149 (MQ=255)
                                                                                                                                               cATAGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGa  >  1:1334224/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCCTGCTCAGCTTTATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAATTGTGGAAAACGTTCGACACCCATCCGGAAGGGTTAAATCAGGCGGA  >  CP009273/4457353‑4457644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑