Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,254 T→A 100% V271V (GTT→GTA)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,2540TA100.0% 836.4 / ‑6.7 258V271V (GTT→GTA) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (139/118);  minor base C (1/0);  total (140/118)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 258 total aligned reads displayed.

CTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGT  >  CP009273/4458109‑4458399
                                                                                                                                                 |                                                                                                                                                 
cTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGt                                                                                                                                                >  1:1176904/1‑149 (MQ=255)
  aCCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGa                                                                                                                                              >  1:1641074/1‑149 (MQ=255)
    cACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGc                                                                                                                                            >  2:1714308/1‑149 (MQ=255)
     aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCa                                                                                                                                           >  1:1520600/1‑149 (MQ=255)
      cTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                          >  1:3628274/1‑149 (MQ=255)
          ccAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaaa                                                                                                                                      >  2:3655109/1‑149 (MQ=255)
          ccAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaaa                                                                                                                                      <  2:3222668/149‑1 (MQ=255)
           cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAg                                                                                                                                     >  2:3537175/1‑149 (MQ=255)
            aGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                    <  2:1431033/149‑1 (MQ=255)
              ccGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGa                                                                                                                                  <  1:3198914/149‑1 (MQ=255)
                     acaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAAt                                                                                                                           <  2:4017229/149‑1 (MQ=255)
                      caGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATg                                                                                                                          >  2:938794/1‑149 (MQ=255)
                        gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGcc                                                                                                                        >  2:3009731/1‑149 (MQ=255)
                        gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGcc                                                                                                                        >  1:1798335/1‑149 (MQ=255)
                        gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGcc                                                                                                                        >  1:2030011/1‑149 (MQ=255)
                          aaTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCtt                                                                                                                      <  1:3583527/149‑1 (MQ=255)
                          aaTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCtt                                                                                                                      <  1:4064903/149‑1 (MQ=255)
                          aaTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCtt                                                                                                                      <  1:1064811/149‑1 (MQ=255)
                                 tGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCaa                                                                                                               <  1:2586700/149‑1 (MQ=255)
                                    aGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAggg                                                                                                            >  2:3828758/1‑149 (MQ=255)
                                       gCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                         <  1:3043849/149‑1 (MQ=255)
                                        cGACACGCTGTGTTTTAGGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCa                                                                                                        <  2:3458625/149‑1 (MQ=255)
                                          acacGCTGTGTTTTCTGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTTCTACCGGTGACACTACCTGGTTTGGTCATCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGc                                                                                                      <  1:2493306/149‑1 (MQ=255)
                                          acacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCTATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGc                                                                                                      <  1:2721883/149‑1 (MQ=255)
                                          acacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGc                                                                                                      >  2:1504037/1‑149 (MQ=255)
                                           cacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGcc                                                                                                     <  1:2311671/149‑1 (MQ=255)
                                           cacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGcc                                                                                                     <  1:1841172/149‑1 (MQ=255)
                                                tgtgTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACACGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTc                                                                                                <  1:3185002/149‑1 (MQ=255)
                                                  tgtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAg                                                                                              >  2:3491707/1‑149 (MQ=255)
                                                   gtttgctGGGCACCACCGTGGTGAGCGGCACGGCACCAGCAATGGTGATTGCTACAGGTGCCAATACCTTGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGt                                                                                             <  2:400492/143‑1 (MQ=255)
                                                   gtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGt                                                                                             >  2:2206694/1‑149 (MQ=255)
                                                     tttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                           <  2:2575229/149‑1 (MQ=255)
                                                       tATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgc                                                                                         <  1:626652/149‑1 (MQ=255)
                                                               ccaccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGAtt                                                                                 <  1:1409964/149‑1 (MQ=255)
                                                               ccaccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGAtt                                                                                 >  1:3898257/1‑149 (MQ=255)
                                                                  ccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGc                                                                              >  2:3025906/1‑149 (MQ=255)
                                                                         gAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGc                                                                       <  2:1333040/149‑1 (MQ=255)
                                                                         gAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGc                                                                       <  2:3758046/149‑1 (MQ=255)
                                                                          aGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCt                                                                      <  1:4027443/149‑1 (MQ=255)
                                                                          aGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCt                                                                      >  2:1883888/1‑149 (MQ=255)
                                                                              gcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTCAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTg                                                                  >  2:158960/1‑149 (MQ=255)
                                                                              gcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTg                                                                  >  2:196135/1‑149 (MQ=255)
                                                                               cacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGa                                                                 <  2:3335/149‑1 (MQ=255)
                                                                                acggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGAt                                                                <  2:3536067/149‑1 (MQ=255)
                                                                                  ggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg                                                              >  1:2019449/1‑149 (MQ=255)
                                                                                   gcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                             >  2:2884471/1‑149 (MQ=255)
                                                                                   gcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATACCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                             >  2:2839453/1‑149 (MQ=255)
                                                                                     acaAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCAc                                                           >  1:819321/1‑149 (MQ=255)
                                                                                     acaAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCAc                                                           <  1:1576976/149‑1 (MQ=255)
                                                                                      caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCAcc                                                          >  2:3130420/1‑149 (MQ=255)
                                                                                      caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCAcc                                                          <  1:2780793/149‑1 (MQ=255)
                                                                                         gCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggt                                                       >  2:2351884/1‑149 (MQ=255)
                                                                                          cAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtg                                                      <  2:96043/149‑1 (MQ=255)
                                                                                           aaTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtgg                                                     >  1:790367/1‑149 (MQ=255)
                                                                                              ggTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGc                                                  <  2:1462264/149‑1 (MQ=255)
                                                                                               gTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCt                                                 >  2:1749700/1‑149 (MQ=255)
                                                                                                  aTTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                              >  2:497214/1‑149 (MQ=255)
                                                                                                  aTTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                              <  2:1951867/149‑1 (MQ=255)
                                                                                                  aTTGCTACAGGTGCCAATACCTGGTGTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                              >  2:108526/1‑149 (MQ=255)
                                                                                                    tGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTaa                                            <  1:135317/149‑1 (MQ=255)
                                                                                                    tGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTaa                                            >  2:3406275/1‑149 (MQ=255)
                                                                                                      cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATc                                          >  1:2199796/1‑149 (MQ=255)
                                                                                                         cAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAAt                                       >  1:3507240/1‑149 (MQ=255)
                                                                                                            gTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGt                                    <  1:1024893/149‑1 (MQ=255)
                                                                                                             tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                   >  1:463034/1‑149 (MQ=255)
                                                                                                             tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                   >  1:462617/1‑149 (MQ=255)
                                                                                                             tGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                   <  2:254089/149‑1 (MQ=255)
                                                                                                                cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTaca                                >  2:2686401/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  1:3396098/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  1:2356262/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  1:2357715/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  1:1345331/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  2:4096536/1‑149 (MQ=255)
                                                                                                                   tACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                             >  2:1599282/1‑149 (MQ=255)
                                                                                                                    aCCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCaa                            >  2:635339/1‑149 (MQ=255)
                                                                                                                    aCCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTGTATGCTGGTGATGGCACCGGTGGTGCTGTTACTCAATGGTTACACCaa                            >  2:3937662/1‑149 (MQ=255)
                                                                                                                      cTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAg                          >  2:2390023/1‑149 (MQ=255)
                                                                                                                      cTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAg                          <  1:3546145/149‑1 (MQ=255)
                                                                                                                       tGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAgg                         >  1:150198/1‑149 (MQ=255)
                                                                                                                         gTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCg                       >  1:7748/1‑149 (MQ=255)
                                                                                                                          tttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGa                      >  1:1098381/1‑149 (MQ=255)
                                                                                                                           ttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                     <  1:1862967/149‑1 (MQ=255)
                                                                                                                            tGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACt                    <  1:3011625/149‑1 (MQ=255)
                                                                                                                              gTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtgg                  >  2:419927/1‑149 (MQ=255)
                                                                                                                              gTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtgg                  >  2:1241413/1‑149 (MQ=255)
                                                                                                                              gTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtgg                  <  1:3358902/149‑1 (MQ=255)
                                                                                                                               tCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtggt                 <  1:3821513/149‑1 (MQ=255)
                                                                                                                                cAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtggtg                <  2:2501374/149‑1 (MQ=255)
                                                                                                                                  aCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTggg              >  1:3552139/1‑149 (MQ=255)
                                                                                                                                   cTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGa             >  2:2125854/1‑149 (MQ=255)
                                                                                                                                        ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcgg        <  1:2676708/149‑1 (MQ=255)
                                                                                                                                        ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcgg        >  2:1533608/1‑149 (MQ=255)
                                                                                                                                        ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcgg        >  2:1314454/1‑149 (MQ=255)
                                                                                                                                         gggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggc       >  2:277135/1‑149 (MQ=255)
                                                                                                                                           gCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGTATCAGCCGGGTCAGTATGCTGCTGATTCGCTTTATGCTGTTGATGGCACGGGTGGTGCTGTTAATCATTGGTTACACCAAAGGCGACTGGTGGGAAGCGgcgc     <  2:1072509/149‑1 (MQ=255)
                                                                                                                                            cGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCt    <  1:911736/149‑1 (MQ=255)
                                                                                                                                             gtgtAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTg   >  1:2165652/1‑149 (MQ=255)
                                                                                                                                              tgtAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGt  <  1:3719590/149‑1 (MQ=255)
                                                                                                                                              tgtAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGt  >  1:669065/1‑149 (MQ=255)
                                                                                                                                              tgtAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGt  >  2:687203/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGT  >  CP009273/4458109‑4458399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑