Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,948 G→T 100% D503Y (GAT→TAT)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,9480GT98.9% 873.1 / 0.1 268D503Y (GAT→TAT) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (129/136);  total (130/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

GGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAAC  >  CP009273/4458803‑4459093
                                                                                                                                                 |                                                                                                                                                 
    gTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCt                                                                                                                                            >  2:1393450/1‑149 (MQ=255)
     tGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTg                                                                                                                                           <  2:453142/149‑1 (MQ=255)
      ggCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGa                                                                                                                                          <  2:1961980/149‑1 (MQ=255)
       gCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGaa                                                                                                                                         <  2:3044076/149‑1 (MQ=255)
       gCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGaa                                                                                                                                         <  2:2282077/149‑1 (MQ=255)
                aCCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAggg                                                                                                                                >  1:2425073/1‑149 (MQ=255)
                   gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCt                                                                                                                             >  1:2881532/1‑149 (MQ=255)
                   gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCt                                                                                                                             <  1:3213109/149‑1 (MQ=255)
                   gAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCt                                                                                                                             <  2:3417960/149‑1 (MQ=255)
                    aGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTg                                                                                                                            >  2:2843495/1‑149 (MQ=255)
                     gCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTgc                                                                                                                           >  2:1207378/1‑149 (MQ=255)
                       aCCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTgcgc                                                                                                                         >  1:624674/1‑149 (MQ=255)
                         cATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGt                                                                                                                       <  1:2412587/149‑1 (MQ=255)
                          aTCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTg                                                                                                                      >  2:3975904/1‑149 (MQ=255)
                           tCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTgg                                                                                                                     >  1:722598/1‑149 (MQ=255)
                               cTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGc                                                                                                                 >  2:2447104/1‑149 (MQ=255)
                                    ttGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTgg                                                                                                            <  2:2938575/149‑1 (MQ=255)
                                         aaGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGcgacg                                                                                                       >  1:2444951/1‑149 (MQ=255)
                                         aaGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGcgacg                                                                                                       >  1:2735696/1‑149 (MQ=255)
                                             tGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAAt                                                                                                   >  2:329585/1‑149 (MQ=255)
                                               cATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATAc                                                                                                 >  1:1477693/1‑149 (MQ=255)
                                                 ttGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCt                                                                                               >  1:1578452/1‑149 (MQ=255)
                                                 ttGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCt                                                                                               >  2:769831/1‑149 (MQ=255)
                                                     aGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCa                                                                                           <  2:3086690/149‑1 (MQ=255)
                                                     aGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCa                                                                                           >  2:1610489/1‑149 (MQ=255)
                                                      ggAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAg                                                                                          >  1:3195336/1‑149 (MQ=255)
                                                        aaaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcg                                                                                        >  2:3866445/1‑149 (MQ=255)
                                                         aaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcgc                                                                                       >  1:3938982/1‑149 (MQ=255)
                                                         aaTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAgcgc                                                                                       <  1:2957180/149‑1 (MQ=255)
                                                          aTCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGcgcg                                                                                      <  1:4100762/149‑1 (MQ=255)
                                                           tCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGt                                                                                     >  2:1790823/1‑149 (MQ=255)
                                                                    tgtgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGAt                                                                            <  1:3513219/149‑1 (MQ=255)
                                                                    tgtgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGAt                                                                            <  2:2881532/149‑1 (MQ=255)
                                                                     gtgtTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGAtt                                                                           >  2:1264179/1‑149 (MQ=255)
                                                                         tCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCa                                                                       >  2:2488426/1‑149 (MQ=255)
                                                                            cAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcg                                                                    <  1:2678928/149‑1 (MQ=255)
                                                                             aGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAATGAGATTACCAgcgg                                                                   <  2:3585325/149‑1 (MQ=255)
                                                                             aGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgg                                                                   >  1:2583144/1‑149 (MQ=255)
                                                                             aGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgg                                                                   <  2:656655/149‑1 (MQ=255)
                                                                             aGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgg                                                                   >  1:2583047/1‑149 (MQ=255)
                                                                              ggTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcggg                                                                  >  2:574682/1‑149 (MQ=255)
                                                                              ggTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcggg                                                                  >  2:2640709/1‑149 (MQ=255)
                                                                                tGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgggcg                                                                >  2:943530/1‑149 (MQ=255)
                                                                                tGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgggcg                                                                >  2:2119771/1‑149 (MQ=255)
                                                                                tGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgggcg                                                                >  2:1048973/1‑149 (MQ=255)
                                                                                tGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAgcgggcg                                                                >  1:3446172/1‑149 (MQ=255)
                                                                                   gTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGAt                                                             >  1:3703470/1‑149 (MQ=255)
                                                                                     cacaATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGATAGGTGTTGCGGTTGATAAGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGa                                                           <  1:4034416/149‑1 (MQ=255)
                                                                                      acaATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGaa                                                          >  1:3876789/1‑149 (MQ=255)
                                                                                            gCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGAc                                                    <  2:3398098/149‑1 (MQ=255)
                                                                                             cGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGAcc                                                   <  1:292461/149‑1 (MQ=255)
                                                                                               agaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTg                                                 >  1:1828294/1‑149 (MQ=255)
                                                                                               agaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTg                                                 >  1:803870/1‑149 (MQ=255)
                                                                                                gaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGa                                                >  1:2148596/1‑149 (MQ=255)
                                                                                                gaTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGa                                                >  1:1682236/1‑149 (MQ=255)
                                                                                                 aTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGAt                                               <  2:3072145/149‑1 (MQ=255)
                                                                                                 aTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGAt                                               <  2:722598/149‑1 (MQ=255)
                                                                                                 aTTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGAt                                               >  1:3097813/1‑149 (MQ=255)
                                                                                                  ttGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATc                                              >  2:2005030/1‑149 (MQ=255)
                                                                                                   tgtgCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATcc                                             <  2:846333/149‑1 (MQ=255)
                                                                                                    gtgCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCt                                            >  1:1140972/1‑149 (MQ=255)
                                                                                                    gtgCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCt                                            <  1:635130/149‑1 (MQ=255)
                                                                                                     tgCCGCTCGATGACATCATGCTGAGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTc                                           <  1:3309802/149‑1 (MQ=255)
                                                                                                        cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGaa                                        <  2:2444951/149‑1 (MQ=255)
                                                                                                        cGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGaa                                        >  1:89279/1‑149 (MQ=255)
                                                                                                          cTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAgg                                      <  1:2348874/149‑1 (MQ=255)
                                                                                                          cTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAgg                                      <  2:337432/149‑1 (MQ=255)
                                                                                                           tCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGa                                     <  2:2433658/149‑1 (MQ=255)
                                                                                                            cGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGat                                    <  1:762124/149‑1 (MQ=255)
                                                                                                             gATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGata                                   <  1:1406108/149‑1 (MQ=255)
                                                                                                             gATGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGata                                   <  1:1300922/149‑1 (MQ=255)
                                                                                                              aTGACATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGatat                                  >  2:2295039/1‑149 (MQ=255)
                                                                                                                 aCATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                               >  2:932938/1‑149 (MQ=255)
                                                                                                                 aCATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                               >  1:1648098/1‑149 (MQ=255)
                                                                                                                 aCATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                               <  1:2923871/149‑1 (MQ=255)
                                                                                                                 aCATCATGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATAtt                               >  1:1190735/1‑149 (MQ=255)
                                                                                                                   atcatGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGAGATATTGc                             >  2:2262230/1‑149 (MQ=255)
                                                                                                                      atGCTGCGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCttt                          <  2:1451185/149‑1 (MQ=255)
                                                                                                                       tgctgcGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCtttt                         <  2:594463/149‑1 (MQ=255)
                                                                                                                         ctgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCt                       >  2:677496/1‑149 (MQ=255)
                                                                                                                         ctgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCt                       <  1:986368/149‑1 (MQ=255)
                                                                                                                          tgcGTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCtt                      >  1:47916/1‑149 (MQ=255)
                                                                                                                             gTAAGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGAt                   >  1:2074386/1‑149 (MQ=255)
                                                                                                                               aaGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATcc                 <  1:24597/149‑1 (MQ=255)
                                                                                                                               aaGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATcc                 <  1:2447060/149‑1 (MQ=255)
                                                                                                                               aaGATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATcc                 <  1:1875784/149‑1 (MQ=255)
                                                                                                                                 gATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGc               >  1:747894/1‑149 (MQ=255)
                                                                                                                                 gATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGc               >  1:4089229/1‑149 (MQ=255)
                                                                                                                                 gATTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGc               >  1:2809363/1‑149 (MQ=255)
                                                                                                                                  aTTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGcc              >  2:2114051/1‑149 (MQ=255)
                                                                                                                                  aTTAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGcc              <  2:2736915/149‑1 (MQ=255)
                                                                                                                                   ttAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCa             <  2:2701796/149‑1 (MQ=255)
                                                                                                                                   ttAAGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCa             <  2:2362941/149‑1 (MQ=255)
                                                                                                                                      aGCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCaaaa          <  1:1264179/149‑1 (MQ=255)
                                                                                                                                       gCGGGTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAag         >  1:142721/1‑149 (MQ=255)
                                                                                                                                         gggTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAagag       >  2:702748/1‑149 (MQ=255)
                                                                                                                                          ggTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAgaga      >  2:2622066/1‑149 (MQ=255)
                                                                                                                                          ggTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAgaga      <  1:1008840/149‑1 (MQ=255)
                                                                                                                                           gTTACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGAc     <  1:1172055/149‑1 (MQ=255)
                                                                                                                                             tACTTATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACaa   <  2:1905610/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCGCAGGTGCGTCACAATGGCGAGATTGTGCCGCTCGATGACATCATGCTGCGTAAGATTAAGCGGGTTACTGATACGCTGAATCGTCAGGGGCTGCGCGTGGTTGCGGTGGCGACGAAATACCTGCCAGCGCGTGAAGGAGATTACCAGCGGGCGGATGAATCCGACCTGATCCTCGAAGGATATATTGCTTTTCTTGATCCGCCAAAAGAGACAAC  >  CP009273/4458803‑4459093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑