Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,465,936 T→A 100% L228* (TTA→TAA)  yjgL → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,465,9360TA99.1% 768.6 / ‑2.6 229L228* (TTA→TAA) yjgLuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (137/90);  total (138/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

CAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTT  >  CP009273/4465790‑4466080
                                                                                                                                                  |                                                                                                                                                
 aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTaaaa                                                                                                                                               <  1:4092173/149‑1 (MQ=255)
 aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTaaaa                                                                                                                                               >  2:1242138/1‑149 (MQ=255)
    aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGa                                                                                                                                            <  2:1682532/149‑1 (MQ=255)
     aaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGAt                                                                                                                                           >  2:744856/1‑149 (MQ=255)
     aaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGAt                                                                                                                                           <  2:3932808/149‑1 (MQ=255)
         aGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATATACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATtctc                                                                                                                                       <  1:333447/149‑1 (MQ=255)
          gcgcGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCa                                                                                                                                      >  2:4053664/1‑149 (MQ=255)
               tCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGt                                                                                                                                 >  2:786496/1‑149 (MQ=255)
                atATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGtt                                                                                                                                <  2:2302854/148‑1 (MQ=255)
                    tAAATAATAATCATGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAAt                                                                                                                            <  2:3796126/149‑1 (MQ=255)
                     aaaTAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATa                                                                                                                           <  1:1231850/149‑1 (MQ=255)
                      aataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATaa                                                                                                                          >  2:2678115/1‑149 (MQ=255)
                       ataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAAc                                                                                                                         <  1:3953779/149‑1 (MQ=255)
                       ataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAAc                                                                                                                         <  2:4101006/149‑1 (MQ=255)
                         aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCAGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTc                                                                                                                       >  2:3257200/1‑149 (MQ=255)
                          ataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCg                                                                                                                      >  2:2180839/1‑149 (MQ=255)
                            aatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCa                                                                                                                    >  2:3019735/1‑149 (MQ=255)
                             atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGGGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAg                                                                                                                   >  2:273099/1‑149 (MQ=255)
                              tCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAgg                                                                                                                  >  2:555526/1‑149 (MQ=255)
                                aGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGaa                                                                                                                >  1:2128535/1‑149 (MQ=255)
                                aGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGaa                                                                                                                <  2:1391381/149‑1 (MQ=255)
                                    gTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaa                                                                                                            <  1:1579171/149‑1 (MQ=255)
                                     ttAAATAATATACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaaa                                                                                                           <  2:2125128/149‑1 (MQ=255)
                                          taatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACAGAAGTTAAATAATATAAAGGGTTTAAATAATCCTCAGGAGTTAAATAATCAGCATAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGta                                                                                                      >  1:103304/1‑148 (MQ=38)
                                            atCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCca                                                                                                    <  1:3090568/149‑1 (MQ=255)
                                             tCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcac                                                                                                   >  1:1139348/1‑149 (MQ=255)
                                              cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca                                                                                                  >  2:1336765/1‑149 (MQ=255)
                                              cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca                                                                                                  >  1:1314613/1‑149 (MQ=255)
                                              atACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca                                                                                                  <  2:3496199/148‑1 (MQ=255)
                                                  aGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAg                                                                                              >  2:1079061/1‑149 (MQ=255)
                                                  aGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAg                                                                                              <  1:3793138/149‑1 (MQ=255)
                                                  aGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAg                                                                                              >  2:3133398/1‑149 (MQ=255)
                                                   gAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGt                                                                                             >  2:1937284/1‑149 (MQ=255)
                                                   gAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGt                                                                                             >  1:374267/1‑149 (MQ=255)
                                                    aaaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGtt                                                                                            <  2:764760/149‑1 (MQ=255)
                                                     aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACATGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCATAATTAAAAAGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATATTCAACAGGAGATa                                                                                           >  1:1374821/1‑149 (MQ=255)
                                                     aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTa                                                                                           >  1:3995355/1‑149 (MQ=255)
                                                            taatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                                                    <  2:1472588/149‑1 (MQ=255)
                                                            taatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                                                    >  1:1892214/1‑149 (MQ=255)
                                                              atCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCg                                                                                  >  1:3910480/1‑149 (MQ=255)
                                                               tCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGc                                                                                 >  2:306043/1‑149 (MQ=255)
                                                               tCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGc                                                                                 >  2:306073/1‑149 (MQ=255)
                                                               tCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGc                                                                                 >  2:2731348/1‑149 (MQ=255)
                                                                    aGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAg                                                                            >  2:2208097/1‑149 (MQ=255)
                                                                     gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGt                                                                           >  2:2375035/1‑149 (MQ=255)
                                                                     gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGt                                                                           >  2:3952631/1‑149 (MQ=255)
                                                                     gAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGt                                                                           >  2:3954715/1‑149 (MQ=255)
                                                                         ttAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTaaa                                                                       <  2:2188613/149‑1 (MQ=255)
                                                                             ataatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAaataat                                                                   <  1:3282276/149‑1 (MQ=255)
                                                                              taatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAAtt                                                                  <  1:1092281/149‑1 (MQ=255)
                                                                               aatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtc                                                                 >  2:3225864/1‑149 (MQ=255)
                                                                                atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtct                                                                <  1:3608211/149‑1 (MQ=255)
                                                                                atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtct                                                                >  1:3382706/1‑149 (MQ=255)
                                                                                atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtct                                                                >  1:3118222/1‑149 (MQ=255)
                                                                                 tCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtctc                                                               >  2:118161/1‑149 (MQ=255)
                                                                                     cTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGa                                                           >  1:2851478/1‑149 (MQ=255)
                                                                                             aaaTAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATa                                                   >  1:3100632/1‑149 (MQ=255)
                                                                                               ataataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAc                                                 >  1:553441/1‑149 (MQ=255)
                                                                                                taataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAct                                                >  1:1121206/1‑149 (MQ=255)
                                                                                                 aataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAActc                                               >  1:946875/1‑149 (MQ=255)
                                                                                                   tataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                             >  1:1590261/1‑149 (MQ=255)
                                                                                                   tataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                             >  2:1125718/1‑149 (MQ=255)
                                                                                                   tataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTa                                             >  1:1022604/1‑149 (MQ=255)
                                                                                                    ataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTaa                                            <  2:1556490/149‑1 (MQ=255)
                                                                                                    ataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTaa                                            >  2:472137/1‑149 (MQ=255)
                                                                                                    ataCAGGGGTTAAAAAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTaa                                            >  1:673599/1‑149 (MQ=255)
                                                                                                      aCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAgg                                          >  2:368001/1‑149 (MQ=255)
                                                                                                       cAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGt                                         >  1:2902185/1‑149 (MQ=255)
                                                                                                       cAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGt                                         >  2:434344/1‑149 (MQ=255)
                                                                                                       cAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGt                                         <  1:3969340/149‑1 (MQ=255)
                                                                                                            gTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGtt                                    >  1:584994/1‑149 (MQ=255)
                                                                                                             ttAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTg                                   >  2:1108359/1‑149 (MQ=255)
                                                                                                               aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTa                                 <  1:1509988/149‑1 (MQ=255)
                                                                                                               aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTa                                 <  2:820200/149‑1 (MQ=255)
                                                                                                               aaaTAATCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTa                                 <  1:3479153/149‑1 (MQ=255)
                                                                                                                aataatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTAc                                >  1:212598/1‑149 (MQ=255)
                                                                                                                  taatCCTCAGTAGTTAAATAATCAGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTAGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTAAAATAACTCTAAGGTGAGTTGTACAg                              <  2:642875/149‑1 (MQ=255)
                                                                                                                  taatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAg                              >  1:3152822/1‑149 (MQ=255)
                                                                                                                   aatCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGt                             >  1:1649978/1‑149 (MQ=255)
                                                                                                                     tCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGttt                           <  2:2811803/149‑1 (MQ=255)
                                                                                                                     tCCTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGttt                           >  2:1928241/1‑149 (MQ=255)
                                                                                                                      ccTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTc                          <  1:1590852/149‑1 (MQ=255)
                                                                                                                       cTCAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCa                         >  1:36667/1‑149 (MQ=255)
                                                                                                                         cAGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAGTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGt                       >  1:2540305/1‑149 (MQ=255)
                                                                                                                          aGGAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGtt                      >  1:2676552/1‑149 (MQ=255)
                                                                                                                          aGGAGTTAAATAAACCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGtt                      <  2:1712578/149‑1 (MQ=255)
                                                                                                                           ggAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTg                     >  1:698775/1‑149 (MQ=255)
                                                                                                                            gAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGa                    <  2:2585186/149‑1 (MQ=255)
                                                                                                                            gAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGa                    <  1:2731348/149‑1 (MQ=255)
                                                                                                                            gAGTTAAATAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGa                    <  2:25087/149‑1 (MQ=255)
                                                                                                                                 aaaTAATCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTa               <  1:1800517/149‑1 (MQ=255)
                                                                                                                                  aataatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTAc              >  2:2766462/1‑149 (MQ=255)
                                                                                                                                    taatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGa            >  1:703193/1‑149 (MQ=255)
                                                                                                                                    taatCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGa            >  1:83154/1‑149 (MQ=255)
                                                                                                                                      atCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGAtt          >  1:1491991/1‑149 (MQ=255)
                                                                                                                                       tCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTa         >  1:3008994/1‑149 (MQ=255)
                                                                                                                                       tCCGCAGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTa         >  1:2139234/1‑149 (MQ=255)
                                                                                                                                            aGAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGt    <  2:3374723/149‑1 (MQ=255)
                                                                                                                                             gAATTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGtt   >  2:3166809/1‑149 (MQ=255)
                                                                                                                                              aaTTAAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGttt  >  2:930458/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGTTGTACAGTTTCAGTTGATTCTACGATTACGGGTTT  >  CP009273/4465790‑4466080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: