Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,159 G→T 100% I165I (ATC→ATA)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,1590GT99.6% 840.5 / ‑4.4 258I165I (ATC→ATA) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (124/132);  total (125/133)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 258 total aligned reads displayed.

GCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG  >  CP009273/362013‑362304
                                                                                                                                                  |                                                                                                                                                 
gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAt                                                                                                                                                 <  2:3819054/149‑1 (MQ=255)
gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAt                                                                                                                                                 >  2:3052882/1‑149 (MQ=255)
 cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAtt                                                                                                                                                <  2:1766984/149‑1 (MQ=255)
  gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTa                                                                                                                                               >  2:2552427/1‑149 (MQ=255)
    tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAt                                                                                                                                             <  1:664732/149‑1 (MQ=255)
           gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGc                                                                                                                                      <  1:2770915/149‑1 (MQ=255)
            aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                     >  1:3677878/1‑149 (MQ=255)
               ttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTc                                                                                                                                  >  1:3849293/1‑149 (MQ=255)
                tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCt                                                                                                                                 <  2:4102471/149‑1 (MQ=255)
                 ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCtt                                                                                                                                <  2:4083101/149‑1 (MQ=255)
                 ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCtt                                                                                                                                <  2:3223136/149‑1 (MQ=255)
                 ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCtt                                                                                                                                <  1:2447039/149‑1 (MQ=255)
                    ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGtt                                                                                                                             >  2:340657/1‑149 (MQ=255)
                    ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGtt                                                                                                                             <  2:1521997/149‑1 (MQ=255)
                     tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTc                                                                                                                            >  2:649873/1‑149 (MQ=255)
                       ccgATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                          >  2:962089/3‑149 (MQ=255)
                        tgctCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCgg                                                                                                                         <  2:2884103/146‑1 (MQ=255)
                        tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCgg                                                                                                                         >  2:2934791/1‑149 (MQ=255)
                          aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                                       >  1:304478/1‑149 (MQ=255)
                              cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaaa                                                                                                                   <  1:1222998/149‑1 (MQ=255)
                              cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaaa                                                                                                                   <  1:2418531/149‑1 (MQ=255)
                                gCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAgg                                                                                                                 >  2:2562791/1‑149 (MQ=255)
                                    cgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccag                                                                                                             <  2:1107477/149‑1 (MQ=255)
                                      gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                           <  2:2334574/149‑1 (MQ=255)
                                      gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                           >  1:1715777/1‑149 (MQ=255)
                                      gTAGTTCTGCCCGTAACCCTCTGTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                           <  1:362423/149‑1 (MQ=255)
                                       tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                          >  1:2730709/1‑149 (MQ=255)
                                        aGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTCACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAATAGGccagccag                                                                                                         >  2:1416460/1‑149 (MQ=255)
                                         gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAgg                                                                                                        >  2:1204667/1‑149 (MQ=255)
                                          ttCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt                                                                                                       >  1:2340244/1‑149 (MQ=255)
                                             tGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAg                                                                                                    <  1:2221704/149‑1 (MQ=255)
                                              gTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAgg                                                                                                   <  1:1188575/149‑1 (MQ=255)
                                               tCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTATATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                  >  2:2307749/1‑149 (MQ=255)
                                                ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGcc                                                                                                 <  2:2175395/149‑1 (MQ=255)
                                                 cGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGccc                                                                                                >  1:4090752/1‑149 (MQ=255)
                                                   tAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGa                                                                                              <  1:3397346/149‑1 (MQ=255)
                                                    aaCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAg                                                                                             >  2:1980168/1‑149 (MQ=255)
                                                        ctctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCgg                                                                                         <  1:796602/149‑1 (MQ=255)
                                                         tctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                        >  2:2882472/1‑149 (MQ=255)
                                                           tttGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGttt                                                                                      <  1:2180702/149‑1 (MQ=255)
                                                           tgtGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGttt                                                                                      <  2:2292528/147‑1 (MQ=255)
                                                                gcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGa                                                                                 <  1:862230/149‑1 (MQ=255)
                                                                 cgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGaa                                                                                <  1:967179/149‑1 (MQ=255)
                                                                 cgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGaa                                                                                <  1:2886321/149‑1 (MQ=255)
                                                                  gcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAg                                                                               >  2:2722280/1‑149 (MQ=255)
                                                                   cgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGc                                                                              <  1:913491/149‑1 (MQ=255)
                                                                    gcgcgcCAGACTGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                             <  1:2925717/149‑1 (MQ=255)
                                                                    gcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCg                                                                             >  2:3572148/1‑149 (MQ=255)
                                                                     cgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCgg                                                                            >  1:3029846/1‑149 (MQ=255)
                                                                      gcgcCAGAAGGGCTTCCAGCTTTAACGGTTCCCTTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGc                                                                           <  1:2204414/149‑1 (MQ=255)
                                                                           aGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACg                                                                      >  1:344708/1‑149 (MQ=255)
                                                                            gAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGt                                                                     >  2:3109009/1‑149 (MQ=255)
                                                                            gAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGt                                                                     >  2:3268474/1‑149 (MQ=255)
                                                                              aTGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCg                                                                   >  1:108894/1‑149 (MQ=255)
                                                                               tGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGc                                                                  >  1:1508974/1‑149 (MQ=255)
                                                                               tGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGc                                                                  >  1:433923/1‑149 (MQ=255)
                                                                                 gCTTCCAGCTTTAACGGGTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGGTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGccc                                                                <  2:2111649/149‑1 (MQ=255)
                                                                                  cTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCg                                                               <  2:1056075/149‑1 (MQ=255)
                                                                                   ttCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGa                                                              >  2:854652/1‑149 (MQ=255)
                                                                                        gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAtt                                                         >  1:3895098/1‑149 (MQ=255)
                                                                                          tttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGc                                                       >  2:3939342/1‑149 (MQ=255)
                                                                                          tttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGc                                                       >  1:3516379/1‑149 (MQ=255)
                                                                                               cGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGt                                                  >  1:1678250/1‑149 (MQ=255)
                                                                                                ggTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTg                                                 <  2:1322034/149‑1 (MQ=255)
                                                                                                    cccGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAg                                             <  1:3464611/149‑1 (MQ=255)
                                                                                                       gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAt                                          >  1:853027/1‑149 (MQ=255)
                                                                                                        tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                         <  1:2562791/149‑1 (MQ=255)
                                                                                                        tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                         <  1:1959283/149‑1 (MQ=255)
                                                                                                        tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                         >  1:2839719/1‑149 (MQ=255)
                                                                                                        tGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                         <  2:2443403/149‑1 (MQ=255)
                                                                                                          ccAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAg                                       <  2:3677878/149‑1 (MQ=255)
                                                                                                           cAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGc                                      <  2:464196/149‑1 (MQ=255)
                                                                                                            aGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCg                                     >  2:720215/1‑149 (MQ=255)
                                                                                                            aGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCg                                     <  1:3783175/149‑1 (MQ=255)
                                                                                                              ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGc                                   <  1:1567953/149‑1 (MQ=255)
                                                                                                               tGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                  <  1:1968224/149‑1 (MQ=255)
                                                                                                                 aTAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGa                                >  1:1599464/1‑149 (MQ=255)
                                                                                                                    gTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAAc                             >  1:2309181/1‑149 (MQ=255)
                                                                                                                     tcatcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACg                            >  2:882108/1‑149 (MQ=255)
                                                                                                                      catcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACgg                           <  1:2552427/149‑1 (MQ=255)
                                                                                                                       atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGt                          >  1:619087/1‑149 (MQ=255)
                                                                                                                       atcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGt                          <  2:2730709/149‑1 (MQ=255)
                                                                                                                        tcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                         <  2:3198417/149‑1 (MQ=255)
                                                                                                                        tcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                         <  2:3798199/149‑1 (MQ=255)
                                                                                                                        tcaCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTg                         <  1:3259596/149‑1 (MQ=255)
                                                                                                                          aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAg                       >  1:1384285/1‑149 (MQ=255)
                                                                                                                            cGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTGTTGTTCCGGGCAAAAGGCCAGCCAGGTAAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATACGCTGCTGAAACGGTGAGTg                     >  2:1139108/1‑149 (MQ=255)
                                                                                                                             ggggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTgg                    >  2:3529866/1‑149 (MQ=255)
                                                                                                                               ggCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGtt                  <  2:1826261/149‑1 (MQ=255)
                                                                                                                                      ggCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTa           >  2:2838538/1‑149 (MQ=255)
                                                                                                                                       gCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTac          <  1:298137/149‑1 (MQ=255)
                                                                                                                                        cTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacc         >  1:3148478/1‑149 (MQ=255)
                                                                                                                                           aCATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTaccacc      >  1:599099/1‑149 (MQ=255)
                                                                                                                                           aCATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTaccacc      >  1:2089527/1‑149 (MQ=255)
                                                                                                                                           aCATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTaccacc      >  1:1011094/1‑149 (MQ=255)
                                                                                                                                             aTTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAt    >  2:444637/1‑149 (MQ=255)
                                                                                                                                              tttCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt   >  1:1323198/1‑149 (MQ=255)
                                                                                                                                              tttCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt   <  2:2251259/149‑1 (MQ=255)
                                                                                                                                              tttCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCAtt   >  1:2893925/1‑149 (MQ=255)
                                                                                                                                               ttCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTg  >  1:3565683/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG  >  CP009273/362013‑362304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑