Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,471,930 T→A 100% P575P (CCA→CCT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,471,9300TA98.8% 791.5 / ‑0.5 239P575P (CCA→CCT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (119/117);  total (120/119)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

GTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGA  >  CP009273/4471784‑4472075
                                                                                                                                                  |                                                                                                                                                 
 tAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGc                                                                                                                                                >  2:3986757/1‑149 (MQ=255)
     cGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCg                                                                                                                                            <  2:1724302/149‑1 (MQ=255)
       ccAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGaa                                                                                                                                          >  1:758059/1‑149 (MQ=255)
       ccAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGaa                                                                                                                                          <  1:1492790/149‑1 (MQ=255)
       ccAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGaa                                                                                                                                          >  1:3112457/1‑149 (MQ=255)
           cgccgcCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATAc                                                                                                                                      <  2:2227142/149‑1 (MQ=255)
           cgccgcCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATAc                                                                                                                                      <  2:1766145/149‑1 (MQ=255)
            gccgccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATAcc                                                                                                                                     >  1:1321335/1‑149 (MQ=255)
            gccgccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATAcc                                                                                                                                     >  1:3370738/1‑149 (MQ=255)
             ccgccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCg                                                                                                                                    >  2:1429358/1‑149 (MQ=255)
               gccAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTc                                                                                                                                  <  1:3186777/149‑1 (MQ=255)
                 cAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCaa                                                                                                                                >  2:3949353/1‑149 (MQ=255)
                 cAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCaa                                                                                                                                <  2:2776364/149‑1 (MQ=255)
                  aGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAc                                                                                                                               >  2:3062437/1‑149 (MQ=255)
                   gggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAcc                                                                                                                              >  1:2559667/1‑149 (MQ=255)
                   gggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGGTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAcc                                                                                                                              >  1:3307016/1‑149 (MQ=255)
                       gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCatat                                                                                                                          <  2:3941419/149‑1 (MQ=255)
                        aaGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGAGTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATc                                                                                                                         >  2:841076/1‑149 (MQ=255)
                          gcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCa                                                                                                                       >  2:3502030/1‑149 (MQ=255)
                          gcgcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCa                                                                                                                       <  1:874717/149‑1 (MQ=255)
                              agcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTgg                                                                                                                   <  2:2798261/149‑1 (MQ=255)
                               gcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                  >  1:1357586/1‑149 (MQ=255)
                               gcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                  <  2:4091261/149‑1 (MQ=255)
                                 gcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTc                                                                                                                <  2:2320862/149‑1 (MQ=255)
                                   gTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGa                                                                                                              >  2:1383094/1‑149 (MQ=255)
                                    tCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGAt                                                                                                             >  1:4003517/1‑149 (MQ=255)
                                     cAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATa                                                                                                            >  1:2105291/1‑149 (MQ=255)
                                       gTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAAc                                                                                                          <  1:709871/149‑1 (MQ=255)
                                        tACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACg                                                                                                         <  2:1597552/149‑1 (MQ=255)
                                          ccGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGtt                                                                                                       <  1:3582173/149‑1 (MQ=255)
                                          ccGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGtt                                                                                                       <  1:3583888/149‑1 (MQ=255)
                                              gCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAccc                                                                                                   >  2:748933/1‑149 (MQ=255)
                                              gCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAccc                                                                                                   >  2:1616295/1‑149 (MQ=255)
                                               cGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCt                                                                                                  <  1:4087405/149‑1 (MQ=255)
                                               cGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCt                                                                                                  <  2:2510834/149‑1 (MQ=255)
                                                ggCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCtt                                                                                                 <  2:4070586/149‑1 (MQ=255)
                                                      aTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAttt                                                                                           >  1:2822427/1‑149 (MQ=255)
                                                       tACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                          >  2:3476463/1‑149 (MQ=255)
                                                       tACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                          >  2:3736752/1‑149 (MQ=255)
                                                        aCCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTgg                                                                                         <  1:1043696/149‑1 (MQ=255)
                                                           gTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACa                                                                                      >  2:2233412/1‑149 (MQ=255)
                                                            ttCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAt                                                                                     >  2:2699381/1‑149 (MQ=255)
                                                               gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtctt                                                                                  <  2:3254000/149‑1 (MQ=255)
                                                                ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttc                                                                                 >  2:3659841/1‑149 (MQ=255)
                                                                 gAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtcttct                                                                                <  1:924807/149‑1 (MQ=255)
                                                                   aCTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTgg                                                                              >  1:2396766/1‑149 (MQ=255)
                                                                     tGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGcc                                                                            <  2:1749910/149‑1 (MQ=255)
                                                                      gCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAGTTGGACATCTTCTGGCCt                                                                           >  2:2881210/1‑149 (MQ=255)
                                                                        ttCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTc                                                                         >  1:3829088/1‑149 (MQ=255)
                                                                             ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcat                                                                    <  1:231151/149‑1 (MQ=255)
                                                                               tGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtcatca                                                                  <  1:990235/149‑1 (MQ=255)
                                                                                    ttACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTCCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAAt                                                             <  1:2895/149‑1 (MQ=255)
                                                                                         gATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGc                                                        <  1:3426203/149‑1 (MQ=255)
                                                                                         gATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGc                                                        >  1:3157779/1‑149 (MQ=255)
                                                                                         gATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGc                                                        >  1:2663775/1‑149 (MQ=255)
                                                                                          aTTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                       <  1:110342/149‑1 (MQ=255)
                                                                                          aTTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                       <  1:132045/149‑1 (MQ=255)
                                                                                           ttttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCg                                                      >  1:3902432/1‑149 (MQ=255)
                                                                                              tGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTc                                                   >  1:1779278/1‑149 (MQ=255)
                                                                                               gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                  <  2:315697/149‑1 (MQ=255)
                                                                                               gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                  >  1:2283973/1‑149 (MQ=255)
                                                                                               gTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                  <  2:2550944/149‑1 (MQ=255)
                                                                                                   gCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTa                                              >  2:2666846/1‑149 (MQ=255)
                                                                                                    ccAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTaa                                             >  2:834222/1‑149 (MQ=255)
                                                                                                       gCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACg                                          >  2:526619/1‑149 (MQ=255)
                                                                                                         tGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgt                                        <  2:3263946/149‑1 (MQ=255)
                                                                                                         tGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgt                                        <  2:492800/149‑1 (MQ=255)
                                                                                                           cGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgtgt                                      >  2:2540451/1‑149 (MQ=255)
                                                                                                               tGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGaa                                  <  2:272535/149‑1 (MQ=255)
                                                                                                                gCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAAc                                 >  2:2931900/1‑149 (MQ=255)
                                                                                                                  atcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACgg                               >  2:2669507/1‑149 (MQ=255)
                                                                                                                   tcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGc                              >  2:415230/1‑149 (MQ=255)
                                                                                                                    catATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCa                             <  2:1132401/149‑1 (MQ=255)
                                                                                                                     atatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAc                            >  2:1449384/1‑149 (MQ=255)
                                                                                                                       atTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCt                          >  2:3434371/1‑149 (MQ=255)
                                                                                                                        ttGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                         >  1:787647/1‑149 (MQ=255)
                                                                                                                        ttGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                         >  2:247386/1‑149 (MQ=255)
                                                                                                                        ttGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTg                         >  2:3386621/1‑149 (MQ=255)
                                                                                                                           ccGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCgg                      >  2:949320/1‑149 (MQ=255)
                                                                                                                            cGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATCTCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGt                     <  1:3827779/149‑1 (MQ=255)
                                                                                                                            cGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGt                     <  2:3519047/149‑1 (MQ=255)
                                                                                                                                 cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc                <  1:3062437/149‑1 (MQ=255)
                                                                                                                                 cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc                <  2:3307016/149‑1 (MQ=255)
                                                                                                                                  gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCa               <  2:1254885/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCATTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              <  2:1540481/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              <  2:1322396/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              <  2:1321234/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              <  2:3370738/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              <  2:1926684/149‑1 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              >  1:3220171/1‑149 (MQ=255)
                                                                                                                                   tttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt              >  1:2483476/1‑149 (MQ=255)
                                                                                                                                    ttttCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAtt             >  1:1545135/1‑149 (MQ=255)
                                                                                                                                      ttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAtttt           >  1:459098/1‑149 (MQ=255)
                                                                                                                                       tCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTc          <  2:1150993/149‑1 (MQ=255)
                                                                                                                                         cagcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCAt        <  1:3502030/149‑1 (MQ=255)
                                                                                                                                          agcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTTGATTTGGACATCATCTGGCCTTAGTCATCAAGAATCAGGCCGTTCATGTAAACTGTGTGGAACTGCACCTGCGGTTTGCCATTTTCATc       >  1:1176607/1‑149 (MQ=255)
                                                                                                                                          agcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATc       >  2:1380412/1‑149 (MQ=255)
                                                                                                                                          agcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATc       >  2:2175013/1‑149 (MQ=255)
                                                                                                                                           gcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt      >  1:834/1‑149 (MQ=255)
                                                                                                                                               ttCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtga  >  2:3162877/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTGA  >  CP009273/4471784‑4472075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑