Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,480,139 G→T 100% A273E (GCG→GAG)  idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,480,1390GT98.3% 746.3 / 3.2 230A273E (GCG→GAG) idnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (114/112);  total (116/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

GGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAAT  >  CP009273/4479993‑4480283
                                                                                                                                                  |                                                                                                                                                
ggTCAACAGTGTTGTTGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCa                                                                                                                                                <  1:868021/149‑1 (MQ=255)
ggTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCa                                                                                                                                                >  1:2503447/1‑149 (MQ=255)
 gTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCaa                                                                                                                                               <  2:1497304/149‑1 (MQ=255)
  tCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAAt                                                                                                                                              <  2:3693791/149‑1 (MQ=255)
   cAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATg                                                                                                                                             >  2:1747198/1‑149 (MQ=255)
   cAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATg                                                                                                                                             <  2:1981359/149‑1 (MQ=255)
    aaCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATgg                                                                                                                                            >  1:378406/1‑149 (MQ=255)
     aCAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCACTGGa                                                                                                                                           >  1:1825232/1‑149 (MQ=255)
       aGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                         <  2:3613544/149‑1 (MQ=255)
       aGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGaaa                                                                                                                                         <  1:3426771/149‑1 (MQ=255)
             gtggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGtt                                                                                                                                   >  1:2576557/1‑149 (MQ=255)
              tggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                  <  1:1026476/149‑1 (MQ=255)
              tggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTc                                                                                                                                  >  2:3649972/1‑149 (MQ=255)
               ggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCt                                                                                                                                 >  1:3065685/1‑149 (MQ=255)
               ggttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCt                                                                                                                                 >  1:3542723/1‑149 (MQ=255)
                gttgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTg                                                                                                                                <  1:3813175/149‑1 (MQ=255)
                  tgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGTCCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGc                                                                                                                              <  1:2701527/149‑1 (MQ=255)
                  tgtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGc                                                                                                                              <  1:3259294/149‑1 (MQ=255)
                   gtgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCa                                                                                                                             <  1:195278/149‑1 (MQ=255)
                    tgATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCAc                                                                                                                            <  1:3682844/149‑1 (MQ=255)
                      aTCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACgg                                                                                                                          >  2:1842682/1‑149 (MQ=255)
                         gTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGa                                                                                                                       >  1:2986836/1‑149 (MQ=255)
                            aTTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGt                                                                                                                    >  2:1540633/1‑149 (MQ=255)
                            aTTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGt                                                                                                                    >  1:4090161/1‑149 (MQ=255)
                               tttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtc                                                                                                                 <  1:3998431/149‑1 (MQ=255)
                                ttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcg                                                                                                                >  2:1312617/1‑149 (MQ=255)
                                ttAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTtcg                                                                                                                >  2:740318/1‑149 (MQ=255)
                                        gCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGc                                                                                                        <  1:447311/149‑1 (MQ=255)
                                          tCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGcac                                                                                                      <  1:4069197/149‑1 (MQ=255)
                                             gCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCAcagc                                                                                                   >  2:4026692/1‑149 (MQ=255)
                                               aaCATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCAcagcag                                                                                                 <  2:3428306/149‑1 (MQ=255)
                                                aCATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGa                                                                                                <  1:1586391/149‑1 (MQ=255)
                                                  aTTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAg                                                                                              >  2:436937/1‑149 (MQ=255)
                                                   tttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                             >  2:3385968/1‑149 (MQ=255)
                                                   tttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                             >  2:1132408/1‑149 (MQ=255)
                                                   tttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                             >  2:843421/1‑149 (MQ=255)
                                                   tttGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCACATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAgc                                                                                             >  2:105318/1‑149 (MQ=255)
                                                      gggCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCa                                                                                          >  2:3113293/1‑149 (MQ=255)
                                                        gcggcCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCAcc                                                                                        >  2:1297618/1‑149 (MQ=255)
                                                                    ccAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATc                                                                            <  1:265653/149‑1 (MQ=255)
                                                                    ccAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATc                                                                            >  1:1245357/1‑149 (MQ=255)
                                                                    ccAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATc                                                                            <  1:265756/149‑1 (MQ=255)
                                                                      aaTATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGt                                                                          >  2:2207380/1‑149 (MQ=255)
                                                                       atatCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTc                                                                         <  1:739219/149‑1 (MQ=255)
                                                                         atCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAt                                                                       <  1:3901059/149‑1 (MQ=255)
                                                                         atCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAt                                                                       <  1:3900030/149‑1 (MQ=255)
                                                                          tCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAtt                                                                      >  2:2611695/1‑149 (MQ=255)
                                                                            aaaaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTgg                                                                    >  2:2095548/1‑149 (MQ=255)
                                                                              aaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTg                                                                  <  1:2172547/149‑1 (MQ=255)
                                                                              aaCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTg                                                                  <  1:93976/149‑1 (MQ=255)
                                                                               aCGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGc                                                                 <  1:1560705/149‑1 (MQ=255)
                                                                                  gggAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCaaa                                                              >  2:41660/1‑149 (MQ=255)
                                                                                    gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAAt                                                            <  1:1357411/149‑1 (MQ=255)
                                                                                    gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAAt                                                            <  2:2131056/149‑1 (MQ=255)
                                                                                    gAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAAt                                                            <  1:3166524/149‑1 (MQ=255)
                                                                                      gTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATac                                                          >  1:3719806/1‑149 (MQ=255)
                                                                                       tAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATaca                                                         >  2:3480812/1‑149 (MQ=255)
                                                                                         aTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACAcc                                                       >  2:803292/1‑149 (MQ=255)
                                                                                         aTTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACAcc                                                       >  2:2862038/1‑149 (MQ=255)
                                                                                           tACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGt                                                     <  2:3259681/149‑1 (MQ=255)
                                                                                            aCGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCTTc                                                    <  2:1923756/149‑1 (MQ=255)
                                                                                            aCGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTc                                                    <  2:2434908/149‑1 (MQ=255)
                                                                                              gCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACAGTCTa                                                  <  1:242238/149‑1 (MQ=255)
                                                                                                 ggCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAAt                                               >  1:2327744/1‑149 (MQ=255)
                                                                                                  gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                              >  2:29737/1‑149 (MQ=255)
                                                                                                  gCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATc                                              <  1:188285/149‑1 (MQ=255)
                                                                                                      ggCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGTGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCggg                                          >  1:3048788/1‑149 (MQ=255)
                                                                                                      ggCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGTGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCggg                                          >  1:990862/1‑149 (MQ=255)
                                                                                                      ggCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCggg                                          >  1:3382619/1‑149 (MQ=255)
                                                                                                       gCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGt                                         >  2:2170704/1‑149 (MQ=255)
                                                                                                        cTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGtt                                        <  1:669909/149‑1 (MQ=255)
                                                                                                              aTCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCt                                  >  2:231636/1‑149 (MQ=255)
                                                                                                                  tttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAgg                              >  2:1348668/1‑149 (MQ=255)
                                                                                                                  tttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAAGCCGGGTTTGCGCTAAgg                              >  2:2285895/1‑149 (MQ=255)
                                                                                                                   ttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAggg                             <  1:621120/149‑1 (MQ=255)
                                                                                                                   ttGCCTGCCAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAggg                             <  1:482686/149‑1 (MQ=255)
                                                                                                                     gcctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTCAGGGCa                           >  1:1532524/1‑149 (MQ=255)
                                                                                                                     gcctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCa                           <  2:138468/149‑1 (MQ=255)
                                                                                                                       ctgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATc                         >  1:2701808/1‑149 (MQ=255)
                                                                                                                        tgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTCAGGGCATCa                        >  2:4047971/1‑149 (MQ=255)
                                                                                                                        tgccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCa                        <  2:241793/149‑1 (MQ=255)
                                                                                                                         gccAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCAc                       >  2:2503616/1‑149 (MQ=255)
                                                                                                                           cAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGc                     >  2:3317018/1‑149 (MQ=255)
                                                                                                                           cAATCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGc                     >  2:2298582/1‑149 (MQ=255)
                                                                                                                            aaTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCa                    >  1:2468251/1‑149 (MQ=255)
                                                                                                                             aTCTCCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAt                   >  2:3925196/1‑149 (MQ=255)
                                                                                                                              tctcCAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCAtt                  <  2:1713293/149‑1 (MQ=255)
                                                                                                                                 ccAGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAAc               >  1:50078/1‑149 (MQ=255)
                                                                                                                                   aGCCCATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTg             <  1:3649184/149‑1 (MQ=255)
                                                                                                                                     cccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCa           <  1:1684224/149‑1 (MQ=255)
                                                                                                                                     cccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCa           >  1:1191075/1‑149 (MQ=255)
                                                                                                                                      ccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt          <  2:2670564/149‑1 (MQ=255)
                                                                                                                                      ccATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt          <  2:473247/149‑1 (MQ=255)
                                                                                                                                      ccATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt          <  1:1101139/149‑1 (MQ=255)
                                                                                                                                      ccATGAAAACACTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAt          <  1:1952209/149‑1 (MQ=255)
                                                                                                                                       cATGGAAACCCTCGATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAtt         >  1:471469/1‑149 (MQ=255)
                                                                                                                                       cATGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCAGTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCAtt         >  1:4079850/1‑149 (MQ=255)
                                                                                                                                         tGAAAACCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTcc       <  1:287363/149‑1 (MQ=255)
                                                                                                                                           aaaaCCCTCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTa     >  1:1585289/1‑149 (MQ=255)
                                                                                                                                              aCCCTCAATGGAAATGTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAaat  <  2:552565/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCGAGGCTTGGGATCATTTGCCTGCCAATCTCCAGCCCATGAAAACCCGCAATGGAAATTTGTTCTGGCACGGCGAGGTTTCGCTCGCGGCACAGCAGAAGCGCACCCATCGCAATATCGTCATTGGTGCAAAATACACCGTCTAAATCCGGGTTTGCGCTAAGGGCATCACGCATTAACTGCATTCCTAAAT  >  CP009273/4479993‑4480283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑