Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,495,859 T→G 100% D252E (GAT→GAG)  yjhC → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,495,8590TG98.6% 862.4 / ‑2.3 215D252E (GAT→GAG) yjhCputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (108/104);  total (109/106)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

CAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAA  >  CP009273/4495714‑4496004
                                                                                                                                                 |                                                                                                                                                 
 aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGc                                                                                                                                               >  1:784951/1‑149 (MQ=255)
 aGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGc                                                                                                                                               >  1:2768145/1‑149 (MQ=255)
     tttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGa                                                                                                                                           >  1:1059830/1‑149 (MQ=255)
     tttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGa                                                                                                                                           >  2:3566184/1‑149 (MQ=255)
     tttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGa                                                                                                                                           >  1:2077775/1‑149 (MQ=255)
      ttGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGaa                                                                                                                                          <  1:130715/149‑1 (MQ=255)
        ggCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAc                                                                                                                                        <  1:2899768/149‑1 (MQ=255)
             tGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAAcagcag                                                                                                                                   <  2:2296828/149‑1 (MQ=255)
              gAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAgg                                                                                                                                  >  1:1104900/1‑149 (MQ=255)
                 gatgatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTc                                                                                                                               >  1:3486067/1‑149 (MQ=255)
                    gatATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACt                                                                                                                            <  2:2096984/149‑1 (MQ=255)
                     atatGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACtt                                                                                                                           >  2:2432280/1‑149 (MQ=255)
                       atGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAg                                                                                                                         <  2:3244681/149‑1 (MQ=255)
                       atGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAg                                                                                                                         <  2:2354205/149‑1 (MQ=255)
                          ttatttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGAt                                                                                                                      >  1:3500962/1‑149 (MQ=255)
                          ttatttatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGAt                                                                                                                      >  1:97602/1‑149 (MQ=255)
                               tatGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCg                                                                                                                 >  2:1088622/1‑149 (MQ=255)
                                   aCCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCa                                                                                                             >  2:1510860/1‑149 (MQ=255)
                                       tGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACa                                                                                                         <  1:2714892/149‑1 (MQ=255)
                                        ggAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACaa                                                                                                        <  2:2658526/149‑1 (MQ=255)
                                        ggAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACaa                                                                                                        <  2:1901562/149‑1 (MQ=255)
                                        ggAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACaa                                                                                                        <  2:1902044/149‑1 (MQ=255)
                                            ttCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCa                                                                                                    >  1:3435943/1‑149 (MQ=255)
                                             tCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAt                                                                                                   >  1:3791220/1‑149 (MQ=255)
                                               ccGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttt                                                                                                 >  2:2924224/1‑149 (MQ=255)
                                                 gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                               >  2:3394298/1‑149 (MQ=255)
                                                 gTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCAttttt                                                                                               >  2:465739/1‑149 (MQ=255)
                                                   cAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTg                                                                                             >  2:1428975/1‑149 (MQ=255)
                                                   cAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTg                                                                                             <  1:4066195/149‑1 (MQ=255)
                                                      gAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTc                                                                                          >  1:125742/1‑149 (MQ=255)
                                                       aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTcc                                                                                         >  1:747934/1‑149 (MQ=255)
                                                       aaaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTcc                                                                                         <  1:3986797/149‑1 (MQ=255)
                                                        aaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCa                                                                                        <  2:704526/149‑1 (MQ=255)
                                                        aaaCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCa                                                                                        <  2:883676/149‑1 (MQ=255)
                                                         aaCGAGCAACCTTAGTGTGGGGGTGTGAATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCAt                                                                                       <  1:1527793/149‑1 (MQ=255)
                                                          aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATg                                                                                      >  1:402075/1‑149 (MQ=255)
                                                          aCTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATg                                                                                      >  2:3322804/1‑149 (MQ=255)
                                                             aGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGaaa                                                                                   >  2:4085609/1‑149 (MQ=255)
                                                              gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAac                                                                                  <  1:2848626/149‑1 (MQ=255)
                                                              gCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAac                                                                                  <  1:2114206/149‑1 (MQ=255)
                                                                aaCCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAacac                                                                                <  1:762006/149‑1 (MQ=255)
                                                                  ccTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaa                                                                              >  1:1062933/1‑149 (MQ=255)
                                                                  ccTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATAATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTGTTGGTCCATGAAAcacac                                                                              >  1:3861198/1‑148 (MQ=255)
                                                                    ttAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaaga                                                                            >  2:2045356/1‑149 (MQ=255)
                                                                       gagTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaaga                                                                         >  2:2722772/1‑149 (MQ=255)
                                                                        agTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACaagaagaa                                                                        <  2:773015/149‑1 (MQ=255)
                                                                          tGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAagaagaaga                                                                      <  1:850431/149‑1 (MQ=255)
                                                                               gAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatg                                                                 <  2:2593215/149‑1 (MQ=255)
                                                                                aGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAgatgatga                                                                <  1:962802/149‑1 (MQ=255)
                                                                                         aaCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaa                                                       >  2:2898482/1‑149 (MQ=255)
                                                                                          aCTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGaaa                                                      <  2:3832246/149‑1 (MQ=255)
                                                                                            tGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAgg                                                    <  2:850473/149‑1 (MQ=255)
                                                                                             ggCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGc                                                   >  2:2226645/1‑149 (MQ=255)
                                                                                             ggCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGc                                                   <  1:3930760/149‑1 (MQ=255)
                                                                                                 ggAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAata                                               <  2:3782355/149‑1 (MQ=255)
                                                                                                  gAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAatat                                              <  1:2432280/149‑1 (MQ=255)
                                                                                                    aCATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTGAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGa                                            <  2:692136/149‑1 (MQ=255)
                                                                                                      aTTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGAcc                                          >  2:3265376/1‑149 (MQ=255)
                                                                                                         aTGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCa                                       <  1:649872/149‑1 (MQ=255)
                                                                                                          tGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAg                                      >  2:3989796/1‑149 (MQ=255)
                                                                                                            tcatcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaa                                    >  1:45057/1‑149 (MQ=255)
                                                                                                             catcatcaATTGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaaa                                   <  2:2809428/149‑1 (MQ=255)
                                                                                                             catcatcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGaaa                                   >  1:2304915/1‑149 (MQ=255)
                                                                                                                 atcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatgga                               >  1:3256103/1‑149 (MQ=255)
                                                                                                                  tcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              >  2:278358/1‑149 (MQ=255)
                                                                                                                  tcaATGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggat                              >  1:3649213/1‑149 (MQ=255)
                                                                                                                    aaTGGAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAatggatgg                            >  1:170169/1‑149 (MQ=255)
                                                                                                                       ggAACTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGgcgc                         <  1:3566184/149‑1 (MQ=255)
                                                                                                                          aCTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtat                      >  2:3151014/1‑149 (MQ=255)
                                                                                                                          aCTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtat                      <  1:2267625/149‑1 (MQ=255)
                                                                                                                           cTAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCtata                     >  2:2049303/1‑149 (MQ=255)
                                                                                                                            tAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCTGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                    >  2:1846811/1‑149 (MQ=255)
                                                                                                                            tAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                    >  2:1782043/1‑149 (MQ=255)
                                                                                                                            tAAAGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAg                    <  2:3564964/149‑1 (MQ=255)
                                                                                                                             aaaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGc                   >  1:288457/1‑149 (MQ=255)
                                                                                                                              aaGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCa                  >  1:3973030/1‑149 (MQ=255)
                                                                                                                               aGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCat                 <  1:1568152/149‑1 (MQ=255)
                                                                                                                               aGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCat                 <  1:104538/149‑1 (MQ=255)
                                                                                                                               aGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCat                 >  1:1907492/1‑149 (MQ=255)
                                                                                                                               aGGCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCat                 >  1:2580448/1‑149 (MQ=255)
                                                                                                                                ggCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCata                <  1:3394298/149‑1 (MQ=255)
                                                                                                                                ggCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCata                <  1:1183496/149‑1 (MQ=255)
                                                                                                                                 gCTCTATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCatat               <  2:1104900/149‑1 (MQ=255)
                                                                                                                                  ctctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATg              >  1:2381933/1‑149 (MQ=255)
                                                                                                                                  ctctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATg              >  1:308756/1‑149 (MQ=255)
                                                                                                                                    ctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGt            <  1:776957/149‑1 (MQ=255)
                                                                                                                                    ctATTAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGt            >  1:816044/1‑149 (MQ=255)
                                                                                                                                       ttAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCAt         >  2:3073157/1‑149 (MQ=255)
                                                                                                                                        tAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATc        <  1:511798/149‑1 (MQ=255)
                                                                                                                                        tAAAATTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATc        <  1:3351832/149‑1 (MQ=255)
                                                                                                                                         aaaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATcc       <  2:3791220/149‑1 (MQ=255)
                                                                                                                                         aaaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATcc       <  1:2651709/149‑1 (MQ=255)
                                                                                                                                         aaaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATcc       <  1:2650666/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  1:1510860/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  1:2603015/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  1:652658/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  2:822590/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  2:1806553/149‑1 (MQ=255)
                                                                                                                                          aaaTTGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCa      <  1:3423277/149‑1 (MQ=255)
                                                                                                                                             ttGAGATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGa   <  2:400366/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CAGGATTTGGCAATGAAGATGATATGTTATTTATGACCTTGGAATTCCCGTCAGGAAAACTAGCAACCTTAGAGTGGGGGAGTGCATTTAACTGGCCGGAACATTATGTCATCATCAATGGAACTAAAGGCTCTATTAAAATTGATATGCAAGAAACAGCAGGGTCACTTAGGATTGGCGGTCAGACAAAGCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAA  >  CP009273/4495714‑4496004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑