Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 40,957 C→A 100% V325V (GTG→GTT)  caiT ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927340,9570CA99.2% 850.8 / ‑2.2 257V325V (GTG→GTT) caiTputative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (149/106);  total (149/108)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

ATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGC  >  CP009273/40811‑41102
                                                                                                                                                  |                                                                                                                                                 
 tcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaacc                                                                                                                                                >  1:2109412/1‑149 (MQ=255)
  cAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccc                                                                                                                                               >  1:2262156/1‑149 (MQ=255)
   aaCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATaaccca                                                                                                                                              <  2:1595667/149‑1 (MQ=255)
      agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACccacca                                                                                                                                           >  1:3508015/1‑149 (MQ=255)
      agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACccacca                                                                                                                                           <  1:3962553/149‑1 (MQ=255)
      agcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACccacca                                                                                                                                           >  1:2849394/1‑149 (MQ=255)
       gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                          >  1:3160647/1‑149 (MQ=255)
       gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                          >  2:2183598/1‑149 (MQ=255)
       gcagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCAt                                                                                                                                          >  2:3502662/1‑149 (MQ=255)
          gagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  1:3018833/1‑149 (MQ=255)
          gagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGcc                                                                                                                                       >  1:2058061/1‑149 (MQ=255)
              gtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGt                                                                                                                                   >  1:951962/1‑149 (MQ=255)
               ttACAACCCAGTACAGTCCAAAGGAACCAGGTTGACGCTGTCAGCACCAGCACCAAGCCGAAGAACAGATCACGCACAGTACGACCAAGGGAGATGCGGCCGAGGAAGATACTCATCTGGATAGCATAAATAACACACCAAGCCCAGTa                                                                                                                                  >  2:3994082/1‑149 (MQ=255)
                tactacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAg                                                                                                                                 >  2:2962023/1‑149 (MQ=255)
                 actacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGa                                                                                                                                >  1:1322806/1‑149 (MQ=255)
                    acCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAaca                                                                                                                             >  2:1777586/1‑149 (MQ=255)
                           aCAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCa                                                                                                                      >  1:2646874/1‑149 (MQ=255)
                            cAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAg                                                                                                                     <  1:2125843/149‑1 (MQ=255)
                              gTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGcc                                                                                                                   <  2:2008545/149‑1 (MQ=255)
                               tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGccc                                                                                                                  <  2:3625449/149‑1 (MQ=255)
                               tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGccc                                                                                                                  <  2:1899068/149‑1 (MQ=255)
                                ccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCt                                                                                                                 >  2:243994/1‑149 (MQ=255)
                                  acaGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                                                                                                               >  1:2541182/1‑149 (MQ=255)
                                  acaGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGc                                                                                                               <  1:3808850/149‑1 (MQ=255)
                                        tCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                         >  2:3748951/1‑149 (MQ=255)
                                        tCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                         >  1:1843852/1‑149 (MQ=255)
                                        tCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                                                                                         >  1:789464/1‑149 (MQ=255)
                                          cAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGACGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgcc                                                                                                       >  2:3081014/1‑149 (MQ=255)
                                          cAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgcc                                                                                                       >  2:3714646/1‑149 (MQ=255)
                                          cAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgcc                                                                                                       >  2:2613308/1‑149 (MQ=255)
                                           aGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccg                                                                                                      >  1:4064361/1‑149 (MQ=255)
                                            ggTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  2:2019192/1‑149 (MQ=255)
                                            ggTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  2:1733107/1‑149 (MQ=255)
                                            ggTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  1:3344423/1‑149 (MQ=255)
                                            ggTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgccgc                                                                                                     >  2:436250/1‑149 (MQ=255)
                                               tGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCtt                                                                                                  <  2:2678476/149‑1 (MQ=255)
                                                          gCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGa                                                                                       <  1:883085/149‑1 (MQ=255)
                                                            cccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATc                                                                                     <  1:565617/149‑1 (MQ=255)
                                                             ccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGACGCCGCCTTTAGCGATGGGATCg                                                                                    >  2:2569055/1‑149 (MQ=255)
                                                             ccAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                                                                                    >  2:2785787/1‑149 (MQ=255)
                                                              cAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCgg                                                                                   >  2:3862046/1‑149 (MQ=255)
                                                              cAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCgg                                                                                   >  2:1819261/1‑149 (MQ=255)
                                                                gCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGta                                                                                 >  1:2065920/1‑149 (MQ=255)
                                                                 cACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGtat                                                                                <  2:1559859/149‑1 (MQ=255)
                                                                     aTGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaa                                                                            <  2:119552/149‑1 (MQ=255)
                                                                        ccGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaacaa                                                                         <  2:1389000/149‑1 (MQ=255)
                                                                          gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       <  1:1190866/149‑1 (MQ=255)
                                                                          gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       >  1:1068854/1‑149 (MQ=255)
                                                                          gAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAacaaca                                                                       >  2:327802/1‑149 (MQ=255)
                                                                            aGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATg                                                                     <  1:1540908/149‑1 (MQ=255)
                                                                             gCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgc                                                                    >  1:1666531/1‑149 (MQ=255)
                                                                             gCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgc                                                                    >  1:1235120/1‑149 (MQ=255)
                                                                                  gTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGc                                                               >  2:3579837/1‑149 (MQ=255)
                                                                                  gTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGc                                                               <  2:1419953/149‑1 (MQ=255)
                                                                                   ttCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCa                                                              <  2:4057313/149‑1 (MQ=255)
                                                                                    tCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAg                                                             >  2:866951/1‑149 (MQ=255)
                                                                                     cACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGa                                                            <  2:2771246/149‑1 (MQ=255)
                                                                                          acaGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACAt                                                       <  2:652238/149‑1 (MQ=255)
                                                                                          acaGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACAt                                                       >  2:3939791/1‑149 (MQ=255)
                                                                                            aGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCa                                                     <  1:3748951/149‑1 (MQ=255)
                                                                                             gTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAg                                                    <  1:2962023/149‑1 (MQ=255)
                                                                                                 gACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAAc                                                >  1:3063301/1‑149 (MQ=255)
                                                                                                 gACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAAc                                                >  1:2047532/1‑149 (MQ=255)
                                                                                                 gACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAAc                                                >  2:2520389/1‑149 (MQ=255)
                                                                                                     aCGGGAGCTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcc                                            <  2:3534430/149‑1 (MQ=255)
                                                                                                      cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATccc                                           <  2:1885918/149‑1 (MQ=255)
                                                                                                      cGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATccc                                           <  1:3328376/149‑1 (MQ=255)
                                                                                                       gggAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATcccc                                          <  1:2017631/149‑1 (MQ=255)
                                                                                                         gagaTGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCAc                                        >  2:3402321/1‑149 (MQ=255)
                                                                                                             tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                    <  1:3109668/149‑1 (MQ=255)
                                                                                                             tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                    >  2:2821070/1‑149 (MQ=255)
                                                                                                             tGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGaa                                    <  1:1056439/149‑1 (MQ=255)
                                                                                                              gCGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAAt                                   >  2:1640959/1‑149 (MQ=255)
                                                                                                               cGGGCGAGGAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATc                                  <  2:1914739/149‑1 (MQ=255)
                                                                                                                  gCGAGGAAGATACTCATCTGGATAGAATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGt                               <  2:3273387/149‑1 (MQ=255)
                                                                                                                      ggAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAag                           >  1:2624722/1‑149 (MQ=255)
                                                                                                                       gAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagt                          >  2:2359734/1‑149 (MQ=255)
                                                                                                                       gAAGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagt                          >  1:2713737/1‑149 (MQ=255)
                                                                                                                        aaGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagta                         >  2:1287639/1‑149 (MQ=255)
                                                                                                                         aGATACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAagtag                        <  1:2784263/149‑1 (MQ=255)
                                                                                                                           aTACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGtt                      >  2:1375263/1‑149 (MQ=255)
                                                                                                                            tACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTc                     >  1:669089/1‑149 (MQ=255)
                                                                                                                            tACTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCACCACCGACTCGGTGAAGTCGTTc                     >  2:3445301/1‑149 (MQ=255)
                                                                                                                             aCTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCa                    >  1:1000211/1‑149 (MQ=255)
                                                                                                                              cTCATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCat                   <  2:1102639/149‑1 (MQ=255)
                                                                                                                                cATCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatga                 <  1:3099054/149‑1 (MQ=255)
                                                                                                                                 aTCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgat                >  2:567920/1‑149 (MQ=255)
                                                                                                                                  tCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgatg               >  2:3341970/1‑149 (MQ=255)
                                                                                                                                  tCTGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCatgatg               <  1:2274766/149‑1 (MQ=255)
                                                                                                                                    tGGATAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGaa             >  2:2410167/1‑149 (MQ=255)
                                                                                                                                      gATAGCATAAATAACCCACCAGGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGc           <  2:800593/149‑1 (MQ=255)
                                                                                                                                       aTAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCt          <  2:319413/149‑1 (MQ=255)
                                                                                                                                       aTAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCt          <  2:2109412/149‑1 (MQ=255)
                                                                                                                                        tAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCTAATCGGTGAAGTAGTTCATGATGAAGCTg         >  1:1949540/1‑149 (MQ=255)
                                                                                                                                        tAGCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTg         <  1:3744231/149‑1 (MQ=255)
                                                                                                                                          gCATAAATAACCCACCCTGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGc       <  2:3018833/149‑1 (MQ=255)
                                                                                                                                          gCATAAATAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGc       >  2:368454/1‑149 (MQ=255)
                                                                                                                                              aaaTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCg   >  1:1568245/1‑149 (MQ=255)
                                                                                                                                               aaTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGc  >  1:4018934/1‑149 (MQ=255)
                                                                                                                                               aaTAACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGc  <  2:1724997/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGCAGATACATCAGCAACATCCCCACCGAATCGGTGAAGTAGTTCATGATGAAGCTGGCACCGC  >  CP009273/40811‑41102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑