Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,531,143 A→T 100% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,531,1430AT99.6% 832.0 / ‑4.6 254I124F (ATT→TTT) fimBtyrosine recombinase/inversion of on/off regulator of fimA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (133/120);  total (133/121)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 261 total aligned reads displayed.

TTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCA  >  CP009273/4530998‑4531288
                                                                                                                                                 |                                                                                                                                                 
ttCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtttta                                                                                                                                                >  1:1657128/1‑149 (MQ=255)
 tCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAATAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAttttat                                                                                                                                               <  2:4058184/149‑1 (MQ=255)
 tCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAttttat                                                                                                                                               >  1:3824861/1‑149 (MQ=255)
    acaacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTAtctc                                                                                                                                            >  1:2290768/1‑149 (MQ=255)
      aacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGa                                                                                                                                          <  2:75094/149‑1 (MQ=255)
       acGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGAc                                                                                                                                         <  2:3643214/149‑1 (MQ=255)
       acGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGAc                                                                                                                                         <  1:1220828/149‑1 (MQ=255)
          cACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTc                                                                                                                                      <  1:2904520/149‑1 (MQ=255)
          cACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTc                                                                                                                                      <  2:1020703/149‑1 (MQ=255)
           aCCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTcc                                                                                                                                     >  1:2830479/1‑149 (MQ=255)
                 tATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCggtggt                                                                                                                               >  2:885113/1‑149 (MQ=255)
                  aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTa                                                                                                                              <  1:1384752/149‑1 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGc                                                                                                                          <  2:3009792/149‑1 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGc                                                                                                                          <  2:952948/149‑1 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGc                                                                                                                          <  1:3552315/149‑1 (MQ=255)
                      aaTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGc                                                                                                                          >  2:154024/1‑149 (MQ=255)
                        tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCg                                                                                                                        <  1:154024/149‑1 (MQ=255)
                         aaaGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCgg                                                                                                                       <  1:1083879/149‑1 (MQ=255)
                          aagaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                      >  2:586886/1‑149 (MQ=255)
                          aagaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCggg                                                                                                                      >  2:368292/1‑149 (MQ=255)
                               gTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTc                                                                                                                 <  2:2799113/149‑1 (MQ=255)
                                 tCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCAc                                                                                                               <  2:1158640/149‑1 (MQ=255)
                                 tCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCAc                                                                                                               <  1:3534107/149‑1 (MQ=255)
                                 tCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCAc                                                                                                               >  2:3536008/1‑149 (MQ=255)
                                     gCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGga                                                                                                           <  2:2829585/149‑1 (MQ=255)
                                      cTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGgag                                                                                                          >  1:2470801/1‑149 (MQ=255)
                                          aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTc                                                                                                      <  1:3225668/149‑1 (MQ=255)
                                          aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTc                                                                                                      >  1:695013/1‑149 (MQ=255)
                                          aaaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTc                                                                                                      <  1:2521273/149‑1 (MQ=255)
                                             aaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                   <  2:2745584/149‑1 (MQ=255)
                                             aaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                   <  2:1586145/149‑1 (MQ=255)
                                             aaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATc                                                                                                   <  1:946722/149‑1 (MQ=255)
                                              aaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATcc                                                                                                  <  2:2714223/149‑1 (MQ=255)
                                                  ggTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGcac                                                                                              >  2:2962754/1‑149 (MQ=255)
                                                     tGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGT‑AGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGt                                                                                          <  1:888626/149‑1 (MQ=255)
                                                        gTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                        >  1:2601007/1‑149 (MQ=255)
                                                         tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAc                                                                                       <  2:550821/149‑1 (MQ=255)
                                                           tCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                     >  1:2385688/1‑149 (MQ=255)
                                                             cGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCa                                                                                   <  2:3361103/149‑1 (MQ=255)
                                                                aCTTCGTACCCGCATGCTGAGCGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTc                                                                                >  2:3725864/1‑149 (MQ=255)
                                                                aCTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTc                                                                                >  2:3300964/1‑149 (MQ=255)
                                                                 cTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCg                                                                               >  1:966848/1‑149 (MQ=255)
                                                                      tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTgg                                                                          >  2:1120414/1‑149 (MQ=255)
                                                                       aCCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGt                                                                         >  1:3691502/1‑149 (MQ=255)
                                                                         ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                       <  2:2560492/149‑1 (MQ=255)
                                                                         ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                       <  2:3584462/149‑1 (MQ=255)
                                                                         ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                       >  1:2721978/1‑149 (MQ=255)
                                                                         ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGttt                                                                       <  2:1539202/149‑1 (MQ=255)
                                                                              tGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                  <  1:1158487/149‑1 (MQ=255)
                                                                              tGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                                  <  1:2245119/149‑1 (MQ=255)
                                                                                  gagaGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                              >  2:1033755/1‑149 (MQ=255)
                                                                                  gagaGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                              <  1:2350967/149‑1 (MQ=255)
                                                                                    gagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTAAGCCATTCGTGTGGTTTTGCTTTGGCGa                                                            >  1:2112070/1‑149 (MQ=255)
                                                                                    gagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATACGCACATTTTACGCCATTCGTGTGGTTTTGCTTTGGCGa                                                            >  1:3875507/1‑149 (MQ=255)
                                                                                      gCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAat                                                          >  1:555197/1‑149 (MQ=255)
                                                                                        gAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                        >  1:2996492/1‑149 (MQ=255)
                                                                                          gTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATgg                                                      <  1:2686198/149‑1 (MQ=255)
                                                                                             ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGaa                                                   <  1:1783708/149‑1 (MQ=255)
                                                                                             ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGaa                                                   >  2:1618762/1‑149 (MQ=255)
                                                                                              gTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                  <  2:1849872/149‑1 (MQ=255)
                                                                                                aTTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAg                                                <  1:2835317/149‑1 (MQ=255)
                                                                                                 tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGa                                               <  2:3433801/149‑1 (MQ=255)
                                                                                                  ttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGAt                                              >  2:1258127/1‑149 (MQ=255)
                                                                                                       tCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcgcg                                         <  1:3953609/149‑1 (MQ=255)
                                                                                                        cACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGa                                        >  2:182281/1‑149 (MQ=255)
                                                                                                         aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                       >  1:3083386/1‑149 (MQ=255)
                                                                                                           gTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACtt                                     >  1:1015994/1‑149 (MQ=255)
                                                                                                            tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTa                                    <  2:2471084/149‑1 (MQ=255)
                                                                                                             aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                   <  2:1797519/149‑1 (MQ=255)
                                                                                                             aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTAt                                   <  2:2046196/149‑1 (MQ=255)
                                                                                                              aGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                                  <  1:2724328/149‑1 (MQ=255)
                                                                                                               ggggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATcc                                 <  2:3328645/149‑1 (MQ=255)
                                                                                                                gggAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCa                                <  2:2278018/149‑1 (MQ=255)
                                                                                                                  gAATCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAgg                              <  2:130659/149‑1 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                            <  2:2503658/149‑1 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                            >  2:3991116/1‑149 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                            >  2:1437106/1‑149 (MQ=255)
                                                                                                                    aTCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAt                            <  2:1161125/149‑1 (MQ=255)
                                                                                                                     tCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                           >  1:1029385/1‑149 (MQ=255)
                                                                                                                     tCCGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGAtt                           >  1:3322454/1‑149 (MQ=255)
                                                                                                                       cGCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTAt                         <  2:2108176/149‑1 (MQ=255)
                                                                                                                        gCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                        >  2:3111509/1‑149 (MQ=255)
                                                                                                                        gCTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                        >  1:2042737/1‑149 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCt                       >  1:1551238/1‑149 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCt                       >  2:1907700/1‑149 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCt                       >  1:3634380/1‑149 (MQ=255)
                                                                                                                         cTTTCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCt                       >  2:1743933/1‑149 (MQ=255)
                                                                                                                          tttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCtt                      <  1:1406544/149‑1 (MQ=255)
                                                                                                                           ttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                     >  1:3473381/1‑149 (MQ=255)
                                                                                                                           ttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                     <  1:1522547/149‑1 (MQ=255)
                                                                                                                           ttCTCGGCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                     <  2:2876951/149‑1 (MQ=255)
                                                                                                                                 gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATc               >  1:3783431/1‑149 (MQ=255)
                                                                                                                                 gCAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATc               >  2:2018869/1‑149 (MQ=255)
                                                                                                                                  cAACAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCg              >  1:718601/1‑149 (MQ=255)
                                                                                                                                   aaCAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGc             >  2:1902752/1‑149 (MQ=255)
                                                                                                                                    aCAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCa            >  2:3365284/1‑149 (MQ=255)
                                                                                                                                     cAGTTTTACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCaa           <  2:927987/149‑1 (MQ=255)
                                                                                                                                         tttACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt       >  1:2099549/1‑149 (MQ=255)
                                                                                                                                           tACCATTTTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAGTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCCTCGCAATATTCg     >  2:2445368/1‑149 (MQ=255)
                                                                                                                                              cATTTTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCa  <  1:645585/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCA  >  CP009273/4530998‑4531288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑