Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,545,608 C→A 100% R177I (AGA→ATA)  yjiC ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,545,6080CA98.0% 780.0 / 5.7 244R177I (AGA→ATA) yjiCuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (118/121);  total (122/122)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

CTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAG  >  CP009273/4545462‑4545753
                                                                                                                                                  |                                                                                                                                                 
cTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATAtt                                                                                                                                                 >  1:229797/1‑149 (MQ=255)
cTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATAtt                                                                                                                                                 >  1:1300578/1‑149 (MQ=255)
   cTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATAttttt                                                                                                                                              <  1:3384881/149‑1 (MQ=255)
     ataCCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGc                                                                                                                                            <  2:2919344/149‑1 (MQ=255)
       aCCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCt                                                                                                                                          >  2:3603326/1‑149 (MQ=255)
        ccAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGACTCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTg                                                                                                                                         >  1:1722487/1‑149 (MQ=255)
            ttattGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCAGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGaa                                                                                                                                     <  2:2102779/149‑1 (MQ=255)
                      aTTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAggg                                                                                                                           <  1:3506858/149‑1 (MQ=255)
                      aTTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAggg                                                                                                                           <  1:92650/149‑1 (MQ=255)
                         aCCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTg                                                                                                                        >  2:2670409/1‑149 (MQ=255)
                          cccAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGa                                                                                                                       >  1:2794655/1‑149 (MQ=255)
                          cccAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGa                                                                                                                       <  1:794983/149‑1 (MQ=255)
                            cAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGaaa                                                                                                                     <  2:3514137/149‑1 (MQ=255)
                              aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAata                                                                                                                   <  2:792359/149‑1 (MQ=255)
                               cTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAatat                                                                                                                  >  1:3463025/1‑149 (MQ=255)
                                 cAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAatatat                                                                                                                >  2:2431939/1‑149 (MQ=255)
                                  aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATc                                                                                                               <  1:3807915/149‑1 (MQ=255)
                                  aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATc                                                                                                               >  2:2290447/1‑149 (MQ=255)
                                   atatTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATcc                                                                                                              >  1:867031/1‑149 (MQ=255)
                                     atTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTa                                                                                                            >  2:2821436/1‑149 (MQ=255)
                                     atTAGCATTAAGTAATGCGGCAAGCGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCTATATAGATATCCATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTa                                                                                                            <  2:2186320/149‑1 (MQ=255)
                                      ttAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTAt                                                                                                           <  2:3271544/149‑1 (MQ=255)
                                       tAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATc                                                                                                          <  1:437999/149‑1 (MQ=255)
                                            ttAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGt                                                                                                     >  1:1634674/1‑149 (MQ=255)
                                              aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTc                                                                                                   <  1:545560/149‑1 (MQ=255)
                                              aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTc                                                                                                   <  1:383397/149‑1 (MQ=255)
                                              aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTc                                                                                                   <  2:949866/149‑1 (MQ=255)
                                               aGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTcc                                                                                                  >  1:2638414/1‑149 (MQ=255)
                                               aGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCATGAGAAGCTGGAAGTGTTGAAATATATCCTATCGTGGTTcc                                                                                                  >  1:1446004/1‑149 (MQ=255)
                                                    tGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCAt                                                                                             <  1:2866259/149‑1 (MQ=255)
                                                     gCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATa                                                                                            >  1:1034841/1‑149 (MQ=255)
                                                     gCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATa                                                                                            <  2:2612618/149‑1 (MQ=255)
                                                      cGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATaa                                                                                           <  1:859656/149‑1 (MQ=255)
                                                       ggCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAc                                                                                          <  2:3282259/149‑1 (MQ=255)
                                                       ggCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAc                                                                                          <  2:3188596/149‑1 (MQ=255)
                                                       ggCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAc                                                                                          <  2:1017693/149‑1 (MQ=255)
                                                        gCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAcc                                                                                         >  1:1006374/1‑149 (MQ=255)
                                                        gCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAAcc                                                                                         >  1:355478/1‑149 (MQ=255)
                                                         cAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCa                                                                                        <  1:2598633/149‑1 (MQ=255)
                                                           aGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                      >  2:895112/1‑149 (MQ=255)
                                                           aGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                      >  2:2685092/1‑149 (MQ=255)
                                                           aGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAgg                                                                                      >  2:2145462/1‑149 (MQ=255)
                                                             ggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGaa                                                                                    <  1:3681583/149‑1 (MQ=255)
                                                             ggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGaa                                                                                    <  1:2164079/149‑1 (MQ=255)
                                                             ggCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGaa                                                                                    >  2:1909568/1‑149 (MQ=255)
                                                              gCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAAc                                                                                   <  1:2431933/149‑1 (MQ=255)
                                                                gAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACAt                                                                                 <  2:971064/149‑1 (MQ=255)
                                                                 aaTCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACAtt                                                                                <  1:3632430/149‑1 (MQ=255)
                                                                  aTCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTg                                                                               >  2:2429339/1‑149 (MQ=255)
                                                                   tCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGc                                                                              >  1:3298529/1‑149 (MQ=255)
                                                                     cGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCaa                                                                            <  2:897830/149‑1 (MQ=255)
                                                                      ggAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAAc                                                                           >  2:1746671/1‑149 (MQ=255)
                                                                       gaagaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAAcc                                                                          <  2:2729158/149‑1 (MQ=255)
                                                                       gaagaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGACAAGCTGGAAGGGTTGACATATATCCTATCGTGGTTCCGGCATAACAAGGAACATTGCAAca                                                                          >  2:3758847/1‑148 (MQ=255)
                                                                        aagaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCa                                                                         <  2:2446162/149‑1 (MQ=255)
                                                                         agaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAg                                                                        <  1:3872929/149‑1 (MQ=255)
                                                                         agaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAg                                                                        >  1:3377065/1‑149 (MQ=255)
                                                                          gaaTTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAgg                                                                       >  1:1243723/1‑149 (MQ=255)
                                                                             tttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGcc                                                                    <  1:2246538/149‑1 (MQ=255)
                                                                                gCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTaa                                                                 <  1:2106456/149‑1 (MQ=255)
                                                                                 cTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAAt                                                                <  1:2762123/149‑1 (MQ=255)
                                                                                  tACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATg                                                               <  1:3956942/149‑1 (MQ=255)
                                                                                  tACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATg                                                               <  1:2830286/149‑1 (MQ=255)
                                                                                   aCCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGt                                                              >  2:1095034/1‑149 (MQ=255)
                                                                                    ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGtt                                                             >  2:1400393/1‑149 (MQ=255)
                                                                                    ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGtt                                                             >  2:3450525/1‑149 (MQ=255)
                                                                                     cccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTa                                                            <  2:3479110/149‑1 (MQ=255)
                                                                                       cTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTAtt                                                          >  1:1044489/1‑149 (MQ=255)
                                                                                       cTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTAtt                                                          <  2:5459/149‑1 (MQ=255)
                                                                                         gTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTc                                                        >  1:2874767/1‑149 (MQ=255)
                                                                                          ttACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCa                                                       >  1:2685080/1‑149 (MQ=255)
                                                                                            aCCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                                                     >  2:4002689/1‑149 (MQ=255)
                                                                                            aCCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                                                     >  1:894754/1‑149 (MQ=255)
                                                                                            aCCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                                                     >  1:2605420/1‑149 (MQ=255)
                                                                                               gTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAAc                                                  >  2:1573049/1‑149 (MQ=255)
                                                                                                     aTCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGtata                                            <  2:62112/149‑1 (MQ=255)
                                                                                                      tCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAg                                           >  1:118770/1‑149 (MQ=255)
                                                                                                      tCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAg                                           >  2:3117191/1‑149 (MQ=255)
                                                                                                      tCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAg                                           >  1:117794/1‑149 (MQ=255)
                                                                                                       cTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGt                                          >  1:1968974/1‑149 (MQ=255)
                                                                                                         gATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTcc                                        <  1:1811509/149‑1 (MQ=255)
                                                                                                               tataGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACa                                  >  1:1668254/1‑149 (MQ=255)
                                                                                                               tataGATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACa                                  >  1:4078535/1‑149 (MQ=255)
                                                                                                                   gATATCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGAt                              <  1:364806/149‑1 (MQ=255)
                                                                                                                    atatCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATc                             <  2:1034841/149‑1 (MQ=255)
                                                                                                                       tCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGaa                          >  2:3032367/1‑149 (MQ=255)
                                                                                                                       tCAATCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGaa                          <  2:2202141/149‑1 (MQ=255)
                                                                                                                          aTCCTTGGCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAgg                       <  1:622919/149‑1 (MQ=255)
                                                                                                                                ggCTGCTCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCtt                 <  1:3324320/149‑1 (MQ=255)
                                                                                                                                 gctgctCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTc                >  1:2674338/1‑149 (MQ=255)
                                                                                                                                  ctgctCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCa               <  1:1751790/149‑1 (MQ=255)
                                                                                                                                    gctCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCata             >  1:2980019/1‑149 (MQ=255)
                                                                                                                                      tCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaa           >  2:126561/1‑149 (MQ=255)
                                                                                                                                      tCATTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaa           <  1:2662664/149‑1 (MQ=255)
                                                                                                                                        aTTGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaata         >  2:3408942/1‑149 (MQ=255)
                                                                                                                                         ttGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCataaataa        >  2:1569326/1‑149 (MQ=255)
                                                                                                                                          tGGTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCAtaaataaa       <  2:1124868/149‑1 (MQ=255)
                                                                                                                                            gTCCATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAAtt     >  2:494218/1‑149 (MQ=255)
                                                                                                                                              ccATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTa   >  1:3739142/1‑149 (MQ=255)
                                                                                                                                               cATATTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAg  >  2:1471142/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAG  >  CP009273/4545462‑4545753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑