Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,547,818 C→A 100% D97Y (GAT→TAT)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,547,8180CA97.7% 730.2 / 5.9 228D97Y (GAT→TAT) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (116/106);  total (119/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

TTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCA  >  CP009273/4547672‑4547961
                                                                                                                                                  |                                                                                                                                               
   aCGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTgc                                                                                                                                            <  1:4088250/149‑1 (MQ=255)
     gCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTgccg                                                                                                                                          >  1:1643189/1‑149 (MQ=255)
        gTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCa                                                                                                                                       >  1:3715857/1‑149 (MQ=255)
         tttATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCAc                                                                                                                                      <  2:3576992/149‑1 (MQ=255)
            aTCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCa                                                                                                                                   <  1:3474669/149‑1 (MQ=255)
             tCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAg                                                                                                                                  >  1:2986211/1‑149 (MQ=255)
             tCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAg                                                                                                                                  <  2:1426394/149‑1 (MQ=255)
              cGACCGCTTCATCGACATCAATAGCTTCAAGTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGc                                                                                                                                 <  2:1682375/149‑1 (MQ=255)
                aCCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGctc                                                                                                                               <  2:3193104/149‑1 (MQ=255)
                 ccGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGctct                                                                                                                              <  1:2576987/149‑1 (MQ=255)
                    cTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGGTGCGCGTAATAGCTGCCGCCACGCAGCTCTGcc                                                                                                                           <  2:2237253/149‑1 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGct                                                                                                                   <  1:143537/149‑1 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGct                                                                                                                   >  1:1652171/1‑149 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGct                                                                                                                   >  2:2911793/1‑149 (MQ=255)
                            aCATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGct                                                                                                                   >  2:3454055/1‑149 (MQ=255)
                             cATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctc                                                                                                                  <  2:1077518/149‑1 (MQ=255)
                              aTCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctct                                                                                                                 <  2:1405018/149‑1 (MQ=255)
                                cactAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctct                                                                                                               <  1:2167533/146‑1 (MQ=255)
                                cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctct                                                                                                               >  1:752694/1‑149 (MQ=255)
                                cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctct                                                                                                               >  1:752538/1‑149 (MQ=255)
                                cAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctct                                                                                                               <  1:131236/149‑1 (MQ=255)
                                 aaTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTa                                                                                                              <  2:3273074/149‑1 (MQ=255)
                                 aaTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTa                                                                                                              <  2:527606/149‑1 (MQ=255)
                                  aTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGctctctca                                                                                                             >  2:2715494/1‑147 (MQ=255)
                                  aTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTaa                                                                                                             <  1:1539001/149‑1 (MQ=255)
                                  aTAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTaa                                                                                                             >  2:2161544/1‑149 (MQ=255)
                                   tAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAAc                                                                                                            <  2:4015542/149‑1 (MQ=255)
                                     gCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTg                                                                                                          <  2:3078770/149‑1 (MQ=255)
                                     gCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTg                                                                                                          >  1:663118/1‑149 (MQ=255)
                                       ttCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGtt                                                                                                        <  2:1140293/149‑1 (MQ=255)
                                           aTTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCaa                                                                                                    <  1:2652135/149‑1 (MQ=255)
                                           aTTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCaa                                                                                                    <  2:2382201/149‑1 (MQ=255)
                                                ccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGAt                                                                                               <  1:2801088/149‑1 (MQ=255)
                                                ccAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGAt                                                                                               <  2:1876469/149‑1 (MQ=255)
                                                  aGGTAAAGAGCGGCGGCATCTGGCTGATAATGGTGGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATgg                                                                                             <  2:1348817/149‑1 (MQ=255)
                                                   ggTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGc                                                                                            >  1:1235656/1‑149 (MQ=255)
                                                   ggTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGc                                                                                            >  1:3283945/1‑149 (MQ=255)
                                                    gTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCg                                                                                           >  1:1636480/1‑149 (MQ=255)
                                                      aaaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                         >  2:3820451/1‑149 (MQ=255)
                                                      aaaGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGa                                                                                         <  2:3797411/149‑1 (MQ=255)
                                                        agagCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATc                                                                                       <  1:3082909/149‑1 (MQ=255)
                                                        agagCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATc                                                                                       <  2:3520812/149‑1 (MQ=255)
                                                         gagCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCt                                                                                      <  1:3061914/149‑1 (MQ=255)
                                                         gagCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCt                                                                                      <  1:573248/149‑1 (MQ=255)
                                                           gcggcgGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGc                                                                                    <  1:2232185/149‑1 (MQ=255)
                                                              gcggcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGaa                                                                                 <  2:2123960/149‑1 (MQ=255)
                                                              gcggcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGaa                                                                                 <  1:3948172/149‑1 (MQ=255)
                                                               cggcATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAAt                                                                                <  1:2100328/149‑1 (MQ=255)
                                                                   aTCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGaa                                                                            >  2:839808/1‑149 (MQ=255)
                                                                    tCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAg                                                                           <  2:2884589/149‑1 (MQ=255)
                                                                        gCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAttt                                                                       <  2:3218301/149‑1 (MQ=255)
                                                                         cTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGAtttt                                                                      >  2:1927347/1‑149 (MQ=255)
                                                                            aTAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTccc                                                                   >  1:1936570/1‑149 (MQ=255)
                                                                              aaTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCtt                                                                 >  1:1698059/1‑149 (MQ=255)
                                                                               aTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                >  1:3364460/1‑149 (MQ=255)
                                                                               aTGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTg                                                                >  2:311416/1‑149 (MQ=255)
                                                                                   aCGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCt                                                            >  2:2482084/1‑149 (MQ=255)
                                                                                   aCGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGAGTTTTCGTTGCGCGTAATAGCTGCCGCCACGCACCACTGCCAGATTGCTCGCTAACTGTTGCAACCGATGGCGGATCTGCGACTGGACGATTTTCCCTGGTTCt                                                            >  1:2927532/1‑149 (MQ=255)
                                                                                      gTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTgag                                                         >  2:3627981/1‑149 (MQ=255)
                                                                                      gTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTgag                                                         >  1:3610861/1‑149 (MQ=255)
                                                                                        aaCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                       >  1:1851803/1‑149 (MQ=255)
                                                                                        aaCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                       >  1:3777473/1‑149 (MQ=255)
                                                                                        aaCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                       >  1:1320348/1‑149 (MQ=255)
                                                                                        aaCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGAGTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGagag                                                       >  1:1733230/1‑149 (MQ=255)
                                                                                         aCAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGt                                                      <  2:263521/149‑1 (MQ=255)
                                                                                              ccGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTATCGTTGCGCGTAATAGCTGCCGACACGCAGCTCTCCCAGATTTCACTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAg                                                 >  1:1305091/1‑149 (MQ=255)
                                                                                                gAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTGTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCGCTAACTGTTGCAACAGATGGCGGATCTGCGACTGGAAGATTTTCCCTTGTGCTGGGAGGTGCAgcg                                               >  1:813787/1‑149 (MQ=255)
                                                                                                  gggAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAgcggc                                             >  1:1954539/1‑149 (MQ=255)
                                                                                                    gAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGt                                           >  2:2946281/1‑149 (MQ=255)
                                                                                                    gAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGt                                           <  2:1027673/149‑1 (MQ=255)
                                                                                                      aaGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTa                                         >  2:1059761/1‑149 (MQ=255)
                                                                                                       agagagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTAc                                        >  2:848935/1‑149 (MQ=255)
                                                                                                         agagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCt                                      <  1:1096875/149‑1 (MQ=255)
                                                                                                          gagTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTg                                     >  2:1607028/1‑149 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACTCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                    <  2:2406687/149‑1 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                    <  1:53573/149‑1 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                    <  2:3353161/149‑1 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                    <  1:2911118/149‑1 (MQ=255)
                                                                                                           agTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTgg                                    <  1:1948092/149‑1 (MQ=255)
                                                                                                              gtgCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCgg                                 <  1:3887010/149‑1 (MQ=255)
                                                                                                                   gCGGCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTaaa                            >  1:1538331/1‑149 (MQ=255)
                                                                                                                     ggCTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACa                          >  1:2866301/1‑149 (MQ=255)
                                                                                                                       cTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAt                        >  2:593164/1‑149 (MQ=255)
                                                                                                                       cTATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAt                        <  2:466143/149‑1 (MQ=255)
                                                                                                                        tATCTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAAtt                       <  1:4042084/149‑1 (MQ=255)
                                                                                                                           cTTGATTTTTCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCaa                    >  2:715108/1‑149 (MQ=255)
                                                                                                                                tttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCa               >  1:188001/1‑149 (MQ=255)
                                                                                                                                tttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCa               >  1:2262598/1‑149 (MQ=255)
                                                                                                                                 ttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCaa              <  2:2449554/149‑1 (MQ=255)
                                                                                                                                 ttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCaa              <  1:730997/149‑1 (MQ=255)
                                                                                                                                 ttttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCaa              >  2:1788503/1‑149 (MQ=255)
                                                                                                                                   ttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATc            <  2:3895772/149‑1 (MQ=255)
                                                                                                                                   ttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATc            <  2:1856662/149‑1 (MQ=255)
                                                                                                                                   ttCGTTGCGCGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATc            <  2:1445144/149‑1 (MQ=255)
                                                                                                                                         gcgcGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGt      >  2:2559299/1‑149 (MQ=255)
                                                                                                                                         gcgcGTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGt      <  2:1615334/149‑1 (MQ=255)
                                                                                                                                           gcgTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTc    >  2:433249/1‑149 (MQ=255)
                                                                                                                                             ntAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCa  >  1:2811736/2‑149 (MQ=255)
                                                                                                                                             gTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCa  >  1:2734477/1‑149 (MQ=255)
                                                                                                                                             gTAATAGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCa  <  2:3568635/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTCACGCAGTTTATCGACCGCTTCATCGACATCAATAGCTTCAATTGCCCAGGTAAAGAGCGGCGGCATCTGGCTGATAATGGACGGTAACAGCCCGAGGGAAAGAGAGTGTGCAGCGGCTATCTTGATTTTTCGTTGCGCGTAATCGCTGCCGCCACGCAGCTCTGCCAGATTGCTCTCTAACTGTTGCAACAGATGGCGGATCTGCGAATGGAAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCA  >  CP009273/4547672‑4547961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑