Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,571,233 G→A 100% S16F (TCT→TTT)  hsdS ← specificity determinant for hsdM and hsdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,571,2330GA99.2% 789.5 / ‑2.8 240S16F (TCT→TTT) hsdSspecificity determinant for hsdM and hsdR
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (148/90);  total (149/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TTTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGC  >  CP009273/4571087‑4571378
                                                                                                                                                  |                                                                                                                                                 
ttttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                 <  2:1924783/149‑1 (MQ=255)
ttttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                 >  1:3470874/1‑149 (MQ=255)
ttttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                 >  1:3471556/1‑149 (MQ=255)
ttcgaGGAACAAAAACCAACGCCGTAGTATCAAACGTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAAt                                                                                                                                                 <  2:3481029/145‑1 (MQ=255)
  ttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATAc                                                                                                                                               <  2:1940808/149‑1 (MQ=255)
     ggAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTgg                                                                                                                                            <  1:628281/149‑1 (MQ=255)
     ggAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTgg                                                                                                                                            >  2:3160943/1‑149 (MQ=255)
      gAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCTAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTggg                                                                                                                                           <  2:4046459/149‑1 (MQ=255)
      gAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTggg                                                                                                                                           <  2:3868186/149‑1 (MQ=255)
          aaaaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGa                                                                                                                                       <  1:2858328/149‑1 (MQ=255)
          aaaaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTNTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGa                                                                                                                                       >  1:2079871/1‑149 (MQ=255)
            aaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATa                                                                                                                                     >  2:1250712/1‑149 (MQ=255)
             aaCCAAGTCCGTAGTATCAAACGTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATaa                                                                                                                                    <  2:1686943/149‑1 (MQ=255)
               ccAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacc                                                                                                                                  >  1:2960858/1‑149 (MQ=255)
                cAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAaccc                                                                                                                                 <  1:1712047/149‑1 (MQ=255)
                 aaGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAaccca                                                                                                                                >  1:1692895/1‑149 (MQ=255)
                 aaGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAaccca                                                                                                                                >  1:1169684/1‑149 (MQ=255)
                  aGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAacccac                                                                                                                               <  1:1793569/149‑1 (MQ=255)
                     ccGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCAcccc                                                                                                                            <  2:1736579/149‑1 (MQ=255)
                      cGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCt                                                                                                                           >  2:2622017/1‑149 (MQ=255)
                       gtagtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTc                                                                                                                          <  2:1674693/149‑1 (MQ=255)
                           taTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGc                                                                                                                      >  2:4000186/1‑149 (MQ=255)
                            aTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCa                                                                                                                     >  2:2482921/1‑149 (MQ=255)
                             tCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCaa                                                                                                                    <  1:3961199/149‑1 (MQ=255)
                              cAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAAt                                                                                                                   <  2:2314764/149‑1 (MQ=255)
                                aaCTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTCAATACTGGGGCGATAACCCACCCCTCCGGCAAttt                                                                                                                 >  2:2919190/1‑149 (MQ=255)
                                 aCTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTc                                                                                                                >  1:3423842/1‑149 (MQ=255)
                                   ttGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTccc                                                                                                              >  2:568770/1‑149 (MQ=255)
                                     gCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCg                                                                                                            >  1:859733/1‑149 (MQ=255)
                                       cATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCa                                                                                                          >  1:7437/1‑149 (MQ=255)
                                            tGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAt                                                                                                     >  1:3172945/1‑149 (MQ=255)
                                            tGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAt                                                                                                     >  2:380591/1‑149 (MQ=255)
                                               atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                  <  1:1987656/149‑1 (MQ=255)
                                               atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                  <  1:3499788/149‑1 (MQ=255)
                                               atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                  <  1:3502999/149‑1 (MQ=255)
                                               atatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                                  >  2:826191/1‑149 (MQ=255)
                                                tatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCt                                                                                                 <  2:3400296/149‑1 (MQ=255)
                                                 atTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCtt                                                                                                >  2:2046738/1‑149 (MQ=255)
                                                   tGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCa                                                                                              >  2:1829806/1‑149 (MQ=255)
                                                       cGCACGTATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAcccc                                                                                          <  2:2346569/149‑1 (MQ=255)
                                                            ggATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCaa                                                                                     >  2:3527752/1‑149 (MQ=255)
                                                                 agagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCCTTCCTTCACCCCACCAAACGcg                                                                                >  1:2884905/1‑148 (MQ=255)
                                                                 agagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGct                                                                                <  2:2243046/149‑1 (MQ=255)
                                                                  gagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                               >  1:445785/1‑149 (MQ=255)
                                                                  gagGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGctt                                                                               >  1:1099808/1‑149 (MQ=255)
                                                                   agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttc                                                                              >  1:2268446/1‑149 (MQ=255)
                                                                   agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttc                                                                              >  1:2250995/1‑149 (MQ=255)
                                                                    ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttct                                                                             >  2:740520/1‑149 (MQ=255)
                                                                     gCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                            <  2:280625/149‑1 (MQ=255)
                                                                     gCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                            <  1:3837922/149‑1 (MQ=255)
                                                                       aaaTAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTcc                                                                          <  1:1472003/149‑1 (MQ=255)
                                                                        aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCa                                                                         <  1:1018009/149‑1 (MQ=255)
                                                                         ataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAAACCACCCCCACCGCAATATCACCCCACACAATCCATAAACCCACCAAAAGCTTCTTACaa                                                                        >  1:1041047/1‑148 (MQ=255)
                                                                          taatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGc                                                                       <  1:1368528/149‑1 (MQ=255)
                                                                           aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCa                                                                      >  2:3129617/1‑149 (MQ=255)
                                                                           aatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCa                                                                      >  2:3053772/1‑149 (MQ=255)
                                                                             tcatcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAAc                                                                    <  1:2622017/149‑1 (MQ=255)
                                                                               atcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCGCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTATTCCAGCAAcag                                                                  >  1:1315337/1‑147 (MQ=255)
                                                                                tcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTATTCCAGCAACTGa                                                                 >  2:2607560/1‑149 (MQ=255)
                                                                                 cTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGAc                                                                <  2:3482679/149‑1 (MQ=255)
                                                                                      aGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGc                                                           <  2:585385/149‑1 (MQ=255)
                                                                                      aGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGc                                                           >  2:32879/1‑149 (MQ=255)
                                                                                         tAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCaaa                                                        <  1:895786/149‑1 (MQ=255)
                                                                                          aaTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAAt                                                       <  2:1374261/149‑1 (MQ=255)
                                                                                           aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATc                                                      <  1:1913066/149‑1 (MQ=255)
                                                                                            tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGTGGCGATAACCCACCAAACCGGCAAATTCCCCGCACTCATTACTTAAACCCAACAAACGATTATTACACCAACTGAAGCTGCAAATCg                                                     >  1:2908199/1‑149 (MQ=255)
                                                                                            tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                     <  2:1556695/149‑1 (MQ=255)
                                                                                            tttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                     <  2:3714425/149‑1 (MQ=255)
                                                                                             ttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCgg                                                    >  2:1008424/1‑149 (MQ=255)
                                                                                              tATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGc                                                   <  1:427885/149‑1 (MQ=255)
                                                                                               aTTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                  >  2:3972106/1‑149 (MQ=255)
                                                                                                ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCt                                                 >  1:3124458/1‑149 (MQ=255)
                                                                                                ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCCGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTACAGCAACTGACGCTGCCAATAGGCCt                                                 >  1:2392545/1‑149 (MQ=255)
                                                                                                 tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTc                                                <  1:212718/149‑1 (MQ=255)
                                                                                                  gCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                               >  2:2782000/1‑149 (MQ=255)
                                                                                                  gCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                               >  2:718365/1‑149 (MQ=255)
                                                                                                   ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAt                                              <  1:2236520/149‑1 (MQ=255)
                                                                                                      gCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgc                                           >  2:2131912/1‑149 (MQ=255)
                                                                                                         attttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgctcg                                        <  1:2553063/148‑1 (MQ=255)
                                                                                                           tttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACCCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcc                                      >  1:1062966/1‑149 (MQ=255)
                                                                                                                 taCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGt                                <  2:222230/149‑1 (MQ=255)
                                                                                                                 taCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGt                                >  1:3779542/1‑149 (MQ=255)
                                                                                                                 taCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCCCACTCATTCCATAAACCCACCAAACGCGTGGTCCAGCAACAGACGCTGCAAAAAGGCCTCATAGCTCGCCCCCAGt                                >  1:2386117/1‑149 (MQ=255)
                                                                                                                  aCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCCAATCGGCCTCATCGCTCGCCCCCAggt                               >  1:2934522/1‑147 (MQ=255)
                                                                                                                  aCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                               >  1:787196/1‑149 (MQ=255)
                                                                                                                    gTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                             <  2:2802915/149‑1 (MQ=255)
                                                                                                                     ttACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCAc                            >  2:1404443/1‑149 (MQ=255)
                                                                                                                     ttACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCATCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAAACGGCCTCATCGCTCGCCCCCAGTTCAc                            >  1:660368/1‑149 (MQ=255)
                                                                                                                      tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCCGTTCACg                           >  2:3195738/1‑149 (MQ=255)
                                                                                                                      tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACg                           >  2:2111857/1‑149 (MQ=255)
                                                                                                                       aCTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGc                          >  2:762246/1‑149 (MQ=255)
                                                                                                                          cctcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATc                       >  2:2660385/1‑149 (MQ=255)
                                                                                                                            tcGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                     <  2:3605817/149‑1 (MQ=255)
                                                                                                                              ggATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCCGTTCACGCATCAgcg                   >  2:3214846/1‑149 (MQ=255)
                                                                                                                                  tAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATc               <  1:951052/149‑1 (MQ=255)
                                                                                                                                   agagTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATcc              >  2:1070080/1‑149 (MQ=255)
                                                                                                                                      gTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGt           <  1:2635604/149‑1 (MQ=255)
                                                                                                                                         gtgACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCa        >  2:501642/1‑149 (MQ=255)
                                                                                                                                           gACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGa      >  2:1488415/1‑149 (MQ=255)
                                                                                                                                              cGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAg   <  2:2978425/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGC  >  CP009273/4571087‑4571378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑