Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,572,520 A→T 100% Y116N (TAC→AAC)  hsdM ← DNA methyltransferase M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,572,5200AT100.0% 761.5 / ‑6.7 226Y116N (TAC→AAC) hsdMDNA methyltransferase M
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (110/115);  minor base C (0/1);  total (110/116)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

TTTCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAAC  >  CP009273/4572374‑4572665
                                                                                                                                                  |                                                                                                                                                 
tttCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTcc                                                                                                                                                 >  2:2561634/1‑149 (MQ=255)
tttCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTcc                                                                                                                                                 <  1:2653430/149‑1 (MQ=255)
 ttCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCa                                                                                                                                                <  1:2136169/149‑1 (MQ=255)
   cagcagATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGt                                                                                                                                              >  2:1743934/1‑149 (MQ=255)
     gcagATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTcc                                                                                                                                            <  2:4075397/149‑1 (MQ=255)
      cagATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCa                                                                                                                                           <  1:727459/149‑1 (MQ=255)
         aTGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCg                                                                                                                                        >  1:2034051/1‑149 (MQ=255)
          tGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGa                                                                                                                                       >  1:3976496/1‑149 (MQ=255)
          tGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGa                                                                                                                                       >  1:2339367/1‑149 (MQ=255)
          tGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGa                                                                                                                                       >  1:3513989/1‑149 (MQ=255)
            aataatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAAt                                                                                                                                     >  1:297943/1‑149 (MQ=255)
             ataatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATc                                                                                                                                    >  1:1825054/1‑149 (MQ=255)
             ataatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATc                                                                                                                                    <  1:2056538/149‑1 (MQ=255)
             ataatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATc                                                                                                                                    >  1:3823825/1‑149 (MQ=255)
             ataatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATc                                                                                                                                    >  1:3520824/1‑149 (MQ=255)
               aatGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCa                                                                                                                                  <  1:135653/149‑1 (MQ=255)
                 ttgTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCata                                                                                                                                >  1:3643846/3‑149 (MQ=255)
                 tGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCata                                                                                                                                >  2:2852915/1‑149 (MQ=255)
                 tGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCata                                                                                                                                <  1:1558269/149‑1 (MQ=255)
                 tGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCata                                                                                                                                <  1:1416775/149‑1 (MQ=255)
                   gTTTTCAGCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATAtt                                                                                                                              <  2:3638749/149‑1 (MQ=255)
                   gTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATAtt                                                                                                                              <  2:3233947/149‑1 (MQ=255)
                   gTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCATGCACCAGACTTGGTTTCATTCGCGTTCTGCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATAtt                                                                                                                              <  2:1588203/149‑1 (MQ=255)
                      ttAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCt                                                                                                                           >  1:884882/1‑149 (MQ=255)
                         aTCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGAc                                                                                                                        <  2:710056/149‑1 (MQ=255)
                         aTCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGAc                                                                                                                        >  2:1418413/1‑149 (MQ=255)
                         aTCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGAc                                                                                                                        >  2:461304/1‑149 (MQ=255)
                          tCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGAcc                                                                                                                       >  1:850306/1‑149 (MQ=255)
                          tCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGAcc                                                                                                                       <  1:3714846/149‑1 (MQ=255)
                            aGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAg                                                                                                                     <  2:541609/149‑1 (MQ=255)
                             gCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGt                                                                                                                    >  2:1295461/1‑149 (MQ=255)
                               ggACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGc                                                                                                                  <  1:3705092/149‑1 (MQ=255)
                                gACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCg                                                                                                                 >  1:1097806/1‑149 (MQ=255)
                                    cgGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTa                                                                                                             <  1:2616770/149‑1 (MQ=255)
                                    cgGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTa                                                                                                             <  1:551069/149‑1 (MQ=255)
                                      gggTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTAtt                                                                                                           <  1:3933601/149‑1 (MQ=255)
                                      gggTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTAtt                                                                                                           >  1:838949/1‑149 (MQ=255)
                                        gTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTg                                                                                                         >  2:2054621/1‑149 (MQ=255)
                                         tGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGTCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGt                                                                                                        <  1:1007990/149‑1 (MQ=255)
                                         tGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGt                                                                                                        <  1:324353/149‑1 (MQ=255)
                                          gAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGtt                                                                                                       <  2:3289372/149‑1 (MQ=255)
                                            aGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTc                                                                                                     <  2:1165971/149‑1 (MQ=255)
                                            aGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTc                                                                                                     <  2:866629/149‑1 (MQ=255)
                                                    ccTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGt                                                                                             <  2:3461890/149‑1 (MQ=255)
                                                       gCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAt                                                                                          <  2:1456993/149‑1 (MQ=255)
                                                        cACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAtg                                                                                         <  1:3496115/149‑1 (MQ=255)
                                                         aCCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAtgg                                                                                        <  1:211713/149‑1 (MQ=255)
                                                          ccAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAtggt                                                                                       <  2:884882/149‑1 (MQ=255)
                                                           cAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAtggtg                                                                                      >  1:3555369/1‑149 (MQ=255)
                                                            aGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAtggtgg                                                                                     <  1:1091868/149‑1 (MQ=255)
                                                             gACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATggtggt                                                                                    >  1:1972744/1‑149 (MQ=255)
                                                             gACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATggtggt                                                                                    >  1:3678328/1‑149 (MQ=255)
                                                                  ggTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTaa                                                                               <  1:3170294/149‑1 (MQ=255)
                                                                     ttCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACAt                                                                            <  1:256475/149‑1 (MQ=255)
                                                                         ttCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAGGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCAATATTGCTGACCAGTGCGGTTATTTGTTTCTGCTCGGTGATGTTGGTACTAACATTaag                                                                        >  1:3811339/1‑147 (MQ=255)
                                                                         ttCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATg                                                                        >  2:3967421/1‑149 (MQ=255)
                                                                         ttCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATg                                                                        >  1:3627426/1‑149 (MQ=255)
                                                                            gcgTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGaaa                                                                     >  1:2717552/1‑149 (MQ=255)
                                                                             cgTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGaaaa                                                                    <  2:2630234/149‑1 (MQ=255)
                                                                               ttcttcTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAAc                                                                  <  2:1056450/149‑1 (MQ=255)
                                                                               ttcttcTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAAc                                                                  >  2:1973961/1‑149 (MQ=255)
                                                                                tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACt                                                                 >  1:3358864/1‑149 (MQ=255)
                                                                                tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACt                                                                 >  2:3696809/1‑149 (MQ=255)
                                                                                    ctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCTAATCCATATTTCTGACCAGTGCGGTTATTTGTTTCGGCTGGTTGATGGTGGTACTAACATTATGAAAAACTGCCt                                                             <  1:1258524/149‑1 (MQ=255)
                                                                                    ctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCt                                                             <  2:809335/149‑1 (MQ=255)
                                                                                      gCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCAGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAAATGCATGt                                                           >  2:3241882/1‑149 (MQ=255)
                                                                                      gCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAANCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGCTGGTGGTACTAACATTATGAAAAACTGCCTGt                                                           >  1:2084932/1‑149 (MQ=255)
                                                                                         aCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTAcc                                                        >  1:484475/1‑149 (MQ=255)
                                                                                         aCAGCCCTTCGTACATAACGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGATAATAGTTTCGGCGCGGTGATGGTGGTACTAACATTATGAAAAACAGCCTGTAcc                                                        >  1:976759/1‑149 (MQ=255)
                                                                                          cAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCa                                                       <  2:168178/149‑1 (MQ=255)
                                                                                            gCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAACGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGc                                                     >  1:2224158/1‑149 (MQ=255)
                                                                                             cccTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCt                                                    <  1:3925568/149‑1 (MQ=255)
                                                                                               cTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCttt                                                  >  2:4018456/1‑149 (MQ=255)
                                                                                                  cGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTg                                               <  1:2051860/149‑1 (MQ=255)
                                                                                                   gTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGt                                              >  2:3499273/1‑149 (MQ=255)
                                                                                                    tACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCCGCTTTTTGTc                                             >  1:2972573/1‑149 (MQ=255)
                                                                                                      cATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCAt                                           <  2:3099952/149‑1 (MQ=255)
                                                                                                       atatCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATc                                          <  2:2330359/149‑1 (MQ=255)
                                                                                                        tatCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCCTATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCt                                         >  2:3312003/1‑149 (MQ=255)
                                                                                                        tatCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCt                                         <  1:3771189/149‑1 (MQ=255)
                                                                                                        tatCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCt                                         >  2:3927709/1‑149 (MQ=255)
                                                                                                         atCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCtt                                        >  2:4093828/1‑149 (MQ=255)
                                                                                                            gCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCAGTACCAGCTTTTTGTCATCTTCGc                                     >  1:1511295/1‑149 (MQ=255)
                                                                                                             ccGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGcc                                    <  2:2141828/149‑1 (MQ=255)
                                                                                                                aaGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTaa                                 >  2:1950288/1‑149 (MQ=255)
                                                                                                                 aGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTaaa                                >  1:2232011/1‑149 (MQ=255)
                                                                                                                 aGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTaaa                                <  1:3003331/149‑1 (MQ=255)
                                                                                                                 aGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTAGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGACTGTACCAGCTTTTTGTCATCATCGCCTaaa                                >  1:2231907/1‑149 (MQ=255)
                                                                                                                     aTCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCa                            <  2:3842206/149‑1 (MQ=255)
                                                                                                                     aTCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCa                            <  2:3976496/149‑1 (MQ=255)
                                                                                                                        gcgcgACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGa                         <  1:2852915/149‑1 (MQ=255)
                                                                                                                         cgcgACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAg                        >  2:3539414/1‑149 (MQ=255)
                                                                                                                                 aCCGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTc                <  1:3870721/149‑1 (MQ=255)
                                                                                                                                   cGTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCgg              <  2:1586059/149‑1 (MQ=255)
                                                                                                                                    gTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGt             <  2:2501608/149‑1 (MQ=255)
                                                                                                                                    gTGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTAGGCTCGGTGATGGTGGTACGAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGt             >  1:9517/1‑149 (MQ=255)
                                                                                                                                     tGCGCGCCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTa            >  1:3768416/1‑149 (MQ=255)
                                                                                                                                      gcgcgcCGTTGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAg           >  1:2864506/1‑149 (MQ=255)
                                                                                                                                              ttGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaactgcaa   >  2:3663711/1‑149 (MQ=255)
                                                                                                                                               tGTTCCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaactgcaac  <  2:299363/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAAC  >  CP009273/4572374‑4572665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑