Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,255 A→C 100% L95L (CTT→CTG)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,2550AC98.5% 1080.4 / ‑0.6 269L95L (CTT→CTG) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (135/129);  total (138/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 269 total aligned reads displayed.

TCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAA  >  CP009273/4584109‑4584398
                                                                                                                                                  |                                                                                                                                               
tCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAg                                                                                                                                               >  1:2187482/1‑149 (MQ=255)
tCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAg                                                                                                                                               >  2:3075887/1‑149 (MQ=255)
  aCCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGtt                                                                                                                                             <  2:42092/149‑1 (MQ=255)
  aCCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGtt                                                                                                                                             <  2:2248413/149‑1 (MQ=255)
    cAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgc                                                                                                                                           >  2:2749336/1‑149 (MQ=255)
     aaTCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTgcg                                                                                                                                          >  2:1141851/1‑149 (MQ=255)
        ccccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCaa                                                                                                                                       >  1:488647/1‑149 (MQ=255)
        ccccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCaa                                                                                                                                       <  1:3433922/149‑1 (MQ=255)
         cccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAt                                                                                                                                      <  1:3076222/149‑1 (MQ=255)
         cccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAt                                                                                                                                      <  1:2488234/149‑1 (MQ=255)
          ccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                     <  1:1200133/149‑1 (MQ=255)
           cATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATcc                                                                                                                                    <  2:2316107/149‑1 (MQ=255)
              aCCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGt                                                                                                                                 >  1:3601869/1‑149 (MQ=255)
                cGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTaa                                                                                                                               <  2:3281935/149‑1 (MQ=255)
                cGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTaa                                                                                                                               <  2:1784501/149‑1 (MQ=255)
                 gATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAg                                                                                                                              >  2:1433739/1‑149 (MQ=255)
                   tACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                            >  2:3392835/1‑149 (MQ=255)
                    aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGa                                                                                                                           <  1:1029341/149‑1 (MQ=255)
                       cACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTg                                                                                                                        >  1:1526466/1‑149 (MQ=255)
                       cACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTg                                                                                                                        >  1:2072176/1‑149 (MQ=255)
                        aCCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                       >  1:2904768/1‑149 (MQ=255)
                         ccAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaa                                                                                                                      >  1:3705723/1‑149 (MQ=255)
                          cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaa                                                                                                                     <  1:914689/149‑1 (MQ=255)
                           aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                    <  1:1769604/149‑1 (MQ=255)
                           aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                    >  1:1508435/1‑149 (MQ=255)
                               cGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACg                                                                                                                <  2:1219699/149‑1 (MQ=255)
                               cGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACg                                                                                                                <  1:929431/149‑1 (MQ=255)
                                  aCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGgag                                                                                                             >  1:721990/1‑149 (MQ=255)
                                  aCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGgag                                                                                                             >  1:2864500/1‑149 (MQ=255)
                                   cTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGgaga                                                                                                            <  2:3673102/149‑1 (MQ=255)
                                    tGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGagag                                                                                                           <  2:1498394/149‑1 (MQ=255)
                                     gCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAAGCCCGTAAGCGAGTGAAAACACGGAGAGc                                                                                                          <  2:611885/149‑1 (MQ=255)
                                         aaaGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAg                                                                                                      >  2:359129/1‑149 (MQ=255)
                                           aGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcg                                                                                                    >  2:35015/1‑149 (MQ=255)
                                            gTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcgc                                                                                                   <  2:3705723/149‑1 (MQ=255)
                                            gTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAgcgc                                                                                                   >  2:1550201/1‑149 (MQ=255)
                                               gtgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATTGTGGGCCGCATGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGa                                                                                                <  2:864752/149‑1 (MQ=255)
                                               gtgCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCCGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGa                                                                                                >  2:3957927/1‑149 (MQ=255)
                                                 gCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAtt                                                                                              >  1:3749988/1‑149 (MQ=255)
                                                   aCCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTc                                                                                            <  2:2185945/149‑1 (MQ=255)
                                                         ccAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCCGGCGTGGGCCTTTCCGATCCATGAGTGGGCCGCAAGGCAGTTGCGAAATCCCGTAAGCGAGTCAAAACACGGAGAGCAACGCGCCGATTTCGGTGGc                                                                                      <  1:16639/149‑1 (MQ=255)
                                                           aGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                    >  2:1748588/1‑149 (MQ=255)
                                                               aTTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTaa                                                                                <  1:2287931/149‑1 (MQ=255)
                                                                 tGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATc                                                                              >  1:591151/1‑149 (MQ=255)
                                                                 tGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATc                                                                              >  1:2658392/1‑149 (MQ=255)
                                                                  gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                             >  1:670105/1‑149 (MQ=255)
                                                                  gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATcc                                                                             <  2:727767/149‑1 (MQ=255)
                                                                       gAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAtt                                                                        <  2:886663/149‑1 (MQ=255)
                                                                       gAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAtt                                                                        <  2:886477/149‑1 (MQ=255)
                                                                        aaCAGTGACCAGAAGAACATCCCCAGTTCCAGCATCCGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                       <  1:1844414/149‑1 (MQ=255)
                                                                        aaCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttc                                                                       <  1:2802455/149‑1 (MQ=255)
                                                                            gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCa                                                                   >  2:2520233/1‑149 (MQ=255)
                                                                            gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCa                                                                   >  2:2556481/1‑149 (MQ=255)
                                                                             tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCAc                                                                  >  2:1888312/1‑149 (MQ=255)
                                                                             tGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCAc                                                                  <  1:2509341/149‑1 (MQ=255)
                                                                               aCCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGa                                                                <  2:3386641/149‑1 (MQ=255)
                                                                                ccAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGaa                                                               >  1:604483/1‑149 (MQ=255)
                                                                                   gaagaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCg                                                            >  2:3624808/1‑149 (MQ=255)
                                                                                    aagaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGt                                                           <  1:322113/149‑1 (MQ=255)
                                                                                    aagaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGt                                                           >  2:2096017/1‑149 (MQ=255)
                                                                                      gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGATCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                         >  1:3799269/1‑149 (MQ=255)
                                                                                      gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                         >  1:1405426/1‑149 (MQ=255)
                                                                                      gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                         >  1:811126/1‑149 (MQ=255)
                                                                                        aCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaaa                                                       <  2:4107158/149‑1 (MQ=255)
                                                                                           tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                    <  2:1811187/149‑1 (MQ=255)
                                                                                           tCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGACACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                    >  2:3314634/1‑149 (MQ=255)
                                                                                             cccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGc                                                  >  1:1135821/1‑149 (MQ=255)
                                                                                              ccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCt                                                 <  1:4009165/149‑1 (MQ=255)
                                                                                                aGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAc                                               <  1:1209696/149‑1 (MQ=255)
                                                                                                aGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAc                                               <  2:2992987/149‑1 (MQ=255)
                                                                                                aGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAc                                               <  2:316336/149‑1 (MQ=255)
                                                                                                   cccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGa                                            <  1:983412/149‑1 (MQ=255)
                                                                                                    ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                           >  2:236558/1‑149 (MQ=255)
                                                                                                    ccAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                           >  2:143851/1‑149 (MQ=255)
                                                                                                     cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACa                                          >  1:1857160/1‑149 (MQ=255)
                                                                                                     cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTAACGACa                                          >  1:2141937/1‑149 (MQ=255)
                                                                                                      aGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAg                                         >  2:1689204/1‑149 (MQ=255)
                                                                                                      aGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAg                                         <  2:2813807/149‑1 (MQ=255)
                                                                                                       gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                        >  1:3903985/1‑149 (MQ=255)
                                                                                                       gCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGc                                        >  2:1122077/1‑149 (MQ=255)
                                                                                                          tCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                     >  1:3378980/1‑149 (MQ=255)
                                                                                                             ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                  >  1:1587345/1‑149 (MQ=255)
                                                                                                             ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                  >  2:173690/1‑149 (MQ=255)
                                                                                                                gTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCgg                               >  1:1213450/1‑149 (MQ=255)
                                                                                                                 tGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                              <  1:2214802/149‑1 (MQ=255)
                                                                                                                 tGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                              <  2:943733/149‑1 (MQ=255)
                                                                                                                  gggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTc                             >  2:2730819/1‑149 (MQ=255)
                                                                                                                   ggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCg                            >  1:4093692/1‑149 (MQ=255)
                                                                                                                   ggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCg                            <  2:852273/149‑1 (MQ=255)
                                                                                                                   ggCCTTTACGAGCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCg                            <  2:1862160/149‑1 (MQ=255)
                                                                                                                      cTTTACGATCCAATCGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                         <  1:3847853/149‑1 (MQ=255)
                                                                                                                      cTTTACGATCCAAGAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                         <  2:127293/149‑1 (MQ=255)
                                                                                                                          aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                     <  2:2704219/149‑1 (MQ=255)
                                                                                                                                cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta               <  2:1253565/149‑1 (MQ=255)
                                                                                                                                 aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAc              >  1:3994207/1‑149 (MQ=255)
                                                                                                                                 aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAc              >  1:1956416/1‑149 (MQ=255)
                                                                                                                                  aTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc             >  2:3434353/1‑149 (MQ=255)
                                                                                                                                       gggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACcgccgc        >  2:3816408/1‑149 (MQ=255)
                                                                                                                                            gCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa   >  1:3040677/1‑149 (MQ=255)
                                                                                                                                             cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaaa  <  1:359037/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAA  >  CP009273/4584109‑4584398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑