Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,381 G→T 100% I53I (ATC→ATA)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,3810GT99.1% 742.2 / ‑2.1 226I53I (ATC→ATA) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (108/116);  total (109/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

TCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATAT  >  CP009273/4584235‑4584526
                                                                                                                                                  |                                                                                                                                                 
tCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                                                                                                                                                 >  1:2498337/1‑149 (MQ=255)
  cactAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta                                                                                                                                               <  2:1254010/146‑1 (MQ=255)
  cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta                                                                                                                                               >  2:1510252/1‑149 (MQ=255)
  cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta                                                                                                                                               >  2:750683/1‑149 (MQ=255)
   aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAc                                                                                                                                              >  1:3994671/1‑149 (MQ=255)
    aTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc                                                                                                                                             >  2:3434405/1‑149 (MQ=255)
    aTAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTAcc                                                                                                                                             <  2:494331/149‑1 (MQ=255)
      aGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgc                                                                                                                                           >  1:2368677/1‑149 (MQ=255)
         gggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACcgccgc                                                                                                                                        >  2:3814800/1‑149 (MQ=255)
             cGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaa                                                                                                                                    >  2:3578592/1‑149 (MQ=255)
              gCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa                                                                                                                                   >  1:2230732/1‑149 (MQ=255)
               cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaaa                                                                                                                                  <  1:359037/149‑1 (MQ=255)
                   ggaGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataat                                                                                                                              <  2:2374438/147‑1 (MQ=255)
                    caGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAataata                                                                                                                             <  2:1405426/148‑1 (MQ=255)
                     aGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAAtaataa                                                                                                                            >  1:2191885/1‑149 (MQ=255)
                          cgcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAacaaca                                                                                                                       <  1:624678/149‑1 (MQ=255)
                           gcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACAt                                                                                                                      <  1:1204927/149‑1 (MQ=255)
                           gcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACAt                                                                                                                      <  1:3392835/149‑1 (MQ=255)
                            cAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATg                                                                                                                     <  1:1328312/149‑1 (MQ=255)
                             aaTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATgg                                                                                                                    <  2:2256894/149‑1 (MQ=255)
                             aaTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTAAATCCCAAATCTTCACGAATCGTTAAATTTGCAACCGACAGCGAACTGCTGTCGAGATAATTTATTACAGCCGCAAAAAATAATaacaaaaggg                                                                                                                    >  2:2781428/1‑144 (MQ=255)
                                cccGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCgg                                                                                                                 <  2:458004/149‑1 (MQ=255)
                                     aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAAAAATAACAACATGGCGGTGGtt                                                                                                            >  2:708148/1‑149 (MQ=255)
                                      aGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGttt                                                                                                           >  2:954117/1‑149 (MQ=255)
                                       gCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTg                                                                                                          >  1:375726/1‑149 (MQ=255)
                                       gCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTg                                                                                                          >  1:364898/1‑149 (MQ=255)
                                          aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACCTGGCGGTGGTTTGAAt                                                                                                       <  2:669514/149‑1 (MQ=255)
                                          aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAAt                                                                                                       >  1:3866044/1‑149 (MQ=255)
                                            tGAAAACCCGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGc                                                                                                     <  1:2500362/149‑1 (MQ=255)
                                             gAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCg                                                                                                    <  2:157816/149‑1 (MQ=255)
                                             gAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCg                                                                                                    >  1:1812707/1‑149 (MQ=255)
                                             gAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCg                                                                                                    <  1:2523205/149‑1 (MQ=255)
                                               aaaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGtt                                                                                                  <  2:3601869/149‑1 (MQ=255)
                                               aaaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGtt                                                                                                  <  2:933353/149‑1 (MQ=255)
                                                aaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGttt                                                                                                 >  2:3670967/1‑149 (MQ=255)
                                                aaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGttt                                                                                                 <  2:3974761/149‑1 (MQ=255)
                                                 acacGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTa                                                                                                >  1:2564699/1‑149 (MQ=255)
                                                   acGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAAt                                                                                              <  1:953865/149‑1 (MQ=255)
                                                   acGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAAt                                                                                              <  1:707391/149‑1 (MQ=255)
                                                    cGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAAtt                                                                                             <  1:1476160/149‑1 (MQ=255)
                                                     ggAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTc                                                                                            >  1:2976752/1‑149 (MQ=255)
                                                     ggAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTc                                                                                            <  2:1587345/149‑1 (MQ=255)
                                                      gagaGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCg                                                                                           >  1:3727477/1‑149 (MQ=255)
                                                      gagaGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCg                                                                                           <  1:3957927/149‑1 (MQ=255)
                                                      gagaGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCg                                                                                           >  1:1683981/1‑149 (MQ=255)
                                                       agagCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCgg                                                                                          <  2:3934469/149‑1 (MQ=255)
                                                              aGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGc                                                                                   >  1:2129340/1‑149 (MQ=255)
                                                                cgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGt                                                                                 <  1:2749336/149‑1 (MQ=255)
                                                                     aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAAc                                                                            >  2:1684883/1‑149 (MQ=255)
                                                                     aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAAc                                                                            >  2:2299361/1‑149 (MQ=255)
                                                                     aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAAc                                                                            <  1:35015/149‑1 (MQ=255)
                                                                      tttCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACt                                                                           >  2:2570657/1‑149 (MQ=255)
                                                                           gTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTTATTCGGGTACTGCGTTGAACTAATcc                                                                      <  1:4070248/149‑1 (MQ=255)
                                                                           gTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATcc                                                                      <  1:3289075/149‑1 (MQ=255)
                                                                             ggCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCAt                                                                    >  2:3029014/1‑149 (MQ=255)
                                                                              gCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATc                                                                   >  2:1344446/1‑149 (MQ=255)
                                                                                aCTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtg                                                                 <  1:1737669/149‑1 (MQ=255)
                                                                                 cTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtgg                                                                >  1:1690539/1‑149 (MQ=255)
                                                                                 cTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtgg                                                                <  2:1101719/149‑1 (MQ=255)
                                                                                    aaTCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtggtgg                                                             >  2:1734961/1‑149 (MQ=255)
                                                                                     aTCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGc                                                            >  1:2685019/1‑149 (MQ=255)
                                                                                       cccAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGGACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCAc                                                          <  1:1122077/149‑1 (MQ=255)
                                                                                          aaTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACggg                                                       <  2:3843081/149‑1 (MQ=255)
                                                                                          aaTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACggg                                                       >  1:2952716/1‑149 (MQ=255)
                                                                                            ttcttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGaa                                                     <  2:3259212/149‑1 (MQ=255)
                                                                                            ttcttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGaa                                                     <  1:815973/149‑1 (MQ=255)
                                                                                            ttcttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGaa                                                     >  1:2617414/1‑149 (MQ=255)
                                                                                                tcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATc                                                 <  2:2136049/149‑1 (MQ=255)
                                                                                                tcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATc                                                 <  1:1867834/149‑1 (MQ=255)
                                                                                                   cGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGc                                              <  2:4093692/149‑1 (MQ=255)
                                                                                                       tCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcgac                                          <  2:2368677/149‑1 (MQ=255)
                                                                                                        cGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcgacg                                         <  2:2211472/149‑1 (MQ=255)
                                                                                                         gTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcgacga                                        >  1:4031759/1‑149 (MQ=255)
                                                                                                         gTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCcgacga                                        >  2:557785/1‑149 (MQ=255)
                                                                                                               tttGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATgg                                  >  2:2043352/1‑149 (MQ=255)
                                                                                                                ttGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGa                                 <  1:1550201/149‑1 (MQ=255)
                                                                                                                 tGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAg                                <  2:3100449/149‑1 (MQ=255)
                                                                                                                   cTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTa                              <  1:1930030/149‑1 (MQ=255)
                                                                                                                    tACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTaa                             >  2:652410/1‑149 (MQ=255)
                                                                                                                    tACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTaa                             <  1:2325573/149‑1 (MQ=255)
                                                                                                                     aCCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTaaa                            <  2:1956416/149‑1 (MQ=255)
                                                                                                                         aCAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaa                        <  1:2730819/149‑1 (MQ=255)
                                                                                                                         aCAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaa                        >  2:762778/1‑149 (MQ=255)
                                                                                                                         aCAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaa                        >  1:2010655/1‑149 (MQ=255)
                                                                                                                            gCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaagaa                     <  2:811126/149‑1 (MQ=255)
                                                                                                                            gCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaagaa                     >  2:3332822/1‑149 (MQ=255)
                                                                                                                            gCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaagaa                     <  2:2871157/149‑1 (MQ=255)
                                                                                                                              gAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcg                   >  1:359678/1‑149 (MQ=255)
                                                                                                                               aaCTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcgc                  <  2:1212254/149‑1 (MQ=255)
                                                                                                                               aaCTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcgc                  >  1:1417543/1‑149 (MQ=255)
                                                                                                                                   gCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGAt              <  2:2617414/149‑1 (MQ=255)
                                                                                                                                     ggTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCg            >  1:627164/1‑149 (MQ=255)
                                                                                                                                         gagaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATgg        <  1:331478/149‑1 (MQ=255)
                                                                                                                                          agaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGt       <  2:3866044/149‑1 (MQ=255)
                                                                                                                                          agaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGt       <  1:96404/149‑1 (MQ=255)
                                                                                                                                            aTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGACTAAATGAAGAACGCGGATCGATGGTGa     >  2:2449001/1‑149 (MQ=255)
                                                                                                                                            aTAATGTATGACCGCCGCAAAAAATAATCCCCACCTGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGa     <  2:2492433/149‑1 (MQ=255)
                                                                                                                                             tAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGat    <  1:173690/149‑1 (MQ=255)
                                                                                                                                               aTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat  >  2:1813396/1‑149 (MQ=255)
                                                                                                                                               aTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGatat  <  2:3751310/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATAT  >  CP009273/4584235‑4584526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑