Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,588,180 G→T 100% S360* (TCA→TAA)  opgB ← phosphoglycerol transferases I and II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,588,1800GT98.0% 810.7 / 5.6 254S360* (TCA→TAA) opgBphosphoglycerol transferases I and II
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base T (97/152);  total (97/157)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

GCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGC  >  CP009273/4588034‑4588325
                                                                                                                                                  |                                                                                                                                                 
   tATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGa                                                                                                                                              <  2:2111869/149‑1 (MQ=255)
    aTTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAt                                                                                                                                             >  2:1323139/1‑149 (MQ=255)
    aTTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAt                                                                                                                                             <  2:685030/149‑1 (MQ=255)
     ttGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAtt                                                                                                                                            <  1:3330062/149‑1 (MQ=255)
     ttGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAtt                                                                                                                                            >  1:1626665/1‑149 (MQ=255)
     ttGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTAAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAtt                                                                                                                                            <  1:2868689/149‑1 (MQ=255)
      tGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTc                                                                                                                                           <  2:1810924/149‑1 (MQ=255)
       gAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGAttag                                                                                                                                          <  2:1975973/149‑3 (MQ=255)
          gTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGtt                                                                                                                                       <  1:3885245/149‑1 (MQ=255)
           tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGttt                                                                                                                                      >  1:1035758/1‑149 (MQ=255)
           tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGttt                                                                                                                                      <  1:3966095/149‑1 (MQ=255)
           tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGttt                                                                                                                                      >  2:510598/1‑149 (MQ=255)
            aTTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTa                                                                                                                                     >  1:146503/1‑149 (MQ=255)
            aTTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTa                                                                                                                                     >  1:1186031/1‑149 (MQ=255)
              ttCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTAcc                                                                                                                                   <  1:536713/149‑1 (MQ=255)
                ccACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTAACGCGCTGAATTAATGATTCGGTTTAACGt                                                                                                                                 >  1:695737/1‑149 (MQ=255)
                    gCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCaaa                                                                                                                             >  1:1772889/1‑149 (MQ=255)
                      cGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAAt                                                                                                                           >  2:1274112/1‑149 (MQ=255)
                        tgttgtGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCa                                                                                                                         <  2:3080264/149‑1 (MQ=255)
                         gttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCat                                                                                                                        <  1:120154/149‑1 (MQ=255)
                         gttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCat                                                                                                                        <  1:120112/149‑1 (MQ=255)
                          ttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCata                                                                                                                       <  1:2204434/149‑1 (MQ=255)
                          ttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCata                                                                                                                       <  2:771118/149‑1 (MQ=255)
                          ttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCata                                                                                                                       <  2:949011/149‑1 (MQ=255)
                           tgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCatat                                                                                                                      <  2:3587859/149‑1 (MQ=255)
                               gaTCGCTAAATGGTCAGTAGAGACGACGATGACTGTATCTTTTAACCACGGTGTCGCTTTGATTTTGTTGATTAACGTCGCGATTTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCTGTTTAGCTTCAAAATCATAttttt                                                                                                                  <  2:552485/148‑1 (MQ=255)
                                aTCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATAtttttt                                                                                                                 <  1:3285999/149‑1 (MQ=255)
                                  cGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGc                                                                                                               <  1:2942967/149‑1 (MQ=255)
                                  cGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGc                                                                                                               <  2:170334/149‑1 (MQ=255)
                                    cTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAGTCGGTTTACCGTCAATAGCATATTTTTTGCgg                                                                                                             >  1:3613016/1‑149 (MQ=255)
                                     tAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGt                                                                                                            <  2:3782141/149‑1 (MQ=255)
                                       aaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                          <  1:2702254/149‑1 (MQ=255)
                                       aaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                          >  2:1172320/1‑149 (MQ=255)
                                       aaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                          <  1:3364808/149‑1 (MQ=255)
                                         tGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGGTTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                        >  2:2374293/1‑149 (MQ=255)
                                          ggTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAg                                                                                                       <  1:4008924/149‑1 (MQ=255)
                                              agaagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTAc                                                                                                   <  2:1466644/149‑1 (MQ=255)
                                              agaagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTAc                                                                                                   <  2:2436257/149‑1 (MQ=255)
                                                 agagACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgag                                                                                                >  1:2662574/1‑149 (MQ=255)
                                                 agagACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgag                                                                                                >  1:596987/1‑149 (MQ=255)
                                                  gagaCGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgaga                                                                                               >  1:2787013/1‑149 (MQ=255)
                                                    gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgagaga                                                                                             <  1:3335004/149‑1 (MQ=255)
                                                    gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgagaga                                                                                             <  2:1095788/149‑1 (MQ=255)
                                                    gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgagaga                                                                                             <  2:1831673/149‑1 (MQ=255)
                                                    gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgagaga                                                                                             <  2:2164898/149‑1 (MQ=255)
                                                     acgacgATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGAt                                                                                            >  2:3170380/1‑149 (MQ=255)
                                                     acgacgATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGAt                                                                                            <  2:2661820/149‑1 (MQ=255)
                                                     acgacgATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGAt                                                                                            <  2:596987/149‑1 (MQ=255)
                                                     acgacgATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGAt                                                                                            <  2:1405888/149‑1 (MQ=255)
                                                       gacgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                          >  1:1768877/1‑149 (MQ=255)
                                                         cgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaaaa                                                                                        <  1:83800/149‑1 (MQ=255)
                                                          gaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAAc                                                                                       <  1:1410591/149‑1 (MQ=255)
                                                           aTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAAcc                                                                                      <  2:2835302/149‑1 (MQ=255)
                                                             gACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCAt                                                                                    <  1:963757/149‑1 (MQ=255)
                                                                 gTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCgg                                                                                >  2:3343976/1‑149 (MQ=255)
                                                                 gTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCgg                                                                                >  2:1808880/1‑149 (MQ=255)
                                                                   aTCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGt                                                                              <  1:1199053/149‑1 (MQ=255)
                                                                    tCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTg                                                                             >  2:2991292/1‑149 (MQ=255)
                                                                    tCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTg                                                                             >  2:990439/1‑149 (MQ=255)
                                                                           aCCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTa                                                                      >  1:3040972/1‑149 (MQ=255)
                                                                            ccACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTAt                                                                     >  1:1030499/1‑149 (MQ=255)
                                                                            ccACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTAt                                                                     >  1:4077713/1‑149 (MQ=255)
                                                                               cGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGa                                                                  >  1:1395887/1‑149 (MQ=255)
                                                                                   gACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGt                                                              >  2:1925744/1‑149 (MQ=255)
                                                                                    aCGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTc                                                             <  2:1603566/149‑1 (MQ=255)
                                                                                        tttGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAggg                                                         <  1:920832/149‑1 (MQ=255)
                                                                                         ttGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGt                                                        <  2:3897783/149‑1 (MQ=255)
                                                                                            aTTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaaa                                                     <  2:1626665/149‑1 (MQ=255)
                                                                                                tgttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGt                                                 <  1:3054168/149‑1 (MQ=255)
                                                                                                    gATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTgaga                                             <  1:2657544/149‑1 (MQ=255)
                                                                                                    gATAAACGTCGCGAACTTCACCACCCAGCAACTTACCGAGCTTAATTACAGATTCGGTTTACAGTCAAAATCAAATTATATGCGGTTACAGGTACGAGATATAAAACCATCAGGGTGATCTGAATCGACTGTCATTGTAAACATTgaga                                             >  2:1080933/1‑149 (MQ=255)
                                                                                                     aTAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGaa                                            <  1:1070591/149‑1 (MQ=255)
                                                                                                     aTAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGaa                                            <  2:4100863/149‑1 (MQ=255)
                                                                                                      tAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAAt                                           <  1:2051306/149‑1 (MQ=255)
                                                                                                       aaaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATc                                          <  2:2700190/149‑1 (MQ=255)
                                                                                                         aCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGc                                        <  1:1933235/149‑1 (MQ=255)
                                                                                                           gTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTg                                      <  1:461337/149‑1 (MQ=255)
                                                                                                           gTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTg                                      <  1:3650875/149‑1 (MQ=255)
                                                                                                            tCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGa                                     >  2:2482038/1‑149 (MQ=255)
                                                                                                              gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAcc                                   <  2:1186031/149‑1 (MQ=255)
                                                                                                               cgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCt                                  >  2:847455/1‑149 (MQ=255)
                                                                                                               cgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCt                                  <  1:3066413/149‑1 (MQ=255)
                                                                                                                 aTGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGa                                <  1:3170380/149‑1 (MQ=255)
                                                                                                                 aTGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGa                                >  2:1534115/1‑149 (MQ=255)
                                                                                                                   gTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGc                              <  1:3429271/149‑1 (MQ=255)
                                                                                                                        ccTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGaa                         <  1:2406593/149‑1 (MQ=255)
                                                                                                                           ggCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGc                      >  2:772426/1‑149 (MQ=255)
                                                                                                                           ggCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGc                      >  1:332253/1‑149 (MQ=255)
                                                                                                                                cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTc                 <  2:3516076/149‑1 (MQ=255)
                                                                                                                                   cTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaaa              <  1:712896/149‑1 (MQ=255)
                                                                                                                                    ttACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAACGCTCTTCAAAc             >  2:2257692/1‑149 (MQ=255)
                                                                                                                                    ttACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAAc             <  1:2548729/149‑1 (MQ=255)
                                                                                                                                      aCCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACtt           <  2:2531101/149‑1 (MQ=255)
                                                                                                                                       ccGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACttt          <  2:1236914/149‑1 (MQ=255)
                                                                                                                                           gcTGAATTTCTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGGTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTcc      <  2:3256405/149‑1 (MQ=255)
                                                                                                                                           gcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTcc      <  1:150217/149‑1 (MQ=255)
                                                                                                                                            cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCa     <  1:1140233/149‑1 (MQ=255)
                                                                                                                                            cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCa     >  2:2212336/1‑149 (MQ=255)
                                                                                                                                               aaTTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGc  >  2:3194030/1‑149 (MQ=255)
                                                                                                                                               aaTTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGc  <  2:1851744/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACGC  >  CP009273/4588034‑4588325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑