Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,615,947 G→T 100% E73D (GAG→GAT)  radA → DNA repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,615,9470GT98.3% 749.1 / 3.4 234E73D (GAG→GAT) radADNA repair protein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (116/114);  total (117/117)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

TGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAG  >  CP009273/4615801‑4616091
                                                                                                                                                  |                                                                                                                                                
tgcagtgcCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCt                                                                                                                                                <  1:463504/149‑1 (MQ=255)
  cagtgcCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGc                                                                                                                                              <  1:3323225/149‑1 (MQ=255)
  cagtgcCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGc                                                                                                                                              <  2:2136640/149‑1 (MQ=255)
       ccTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGt                                                                                                                                         >  2:3000697/1‑149 (MQ=255)
         tGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGttt                                                                                                                                       <  2:1023924/149‑1 (MQ=255)
           tCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGttttt                                                                                                                                     >  1:797881/1‑149 (MQ=255)
            cATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTc                                                                                                                                    <  1:2941036/149‑1 (MQ=255)
             aTGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCATTGGTGGCGCGTAACGCGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTcc                                                                                                                                   <  1:2570989/149‑1 (MQ=255)
             aTGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTcc                                                                                                                                   <  2:2858356/149‑1 (MQ=255)
              tGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGCGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCa                                                                                                                                  <  1:2981143/149‑1 (MQ=255)
               gCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCAc                                                                                                                                 <  1:1728385/149‑1 (MQ=255)
                  tGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCgg                                                                                                                              >  2:2558840/1‑149 (MQ=255)
                   ggAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGa                                                                                                                             >  2:284230/1‑149 (MQ=255)
                   ggAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGa                                                                                                                             >  1:1445137/1‑149 (MQ=255)
                   ggAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGa                                                                                                                             >  1:987756/1‑149 (MQ=255)
                   ggAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGa                                                                                                                             >  2:2563218/1‑149 (MQ=255)
                           aTCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAaga                                                                                                                     >  2:3292138/1‑149 (MQ=255)
                           aTCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAaga                                                                                                                     <  2:3133352/149‑1 (MQ=255)
                            tCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAagag                                                                                                                    <  2:632121/149‑1 (MQ=255)
                             cACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGt                                                                                                                   <  2:3010093/149‑1 (MQ=255)
                              cccGAGGTGCGTCTTGCTGCGTCGCCAAGGGTGGCGCTTAACGAGCGTCTCTGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGtt                                                                                                                  <  1:360572/148‑1 (MQ=255)
                                 gAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTTCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGa                                                                                                               >  1:3091942/1‑149 (MQ=255)
                                 gAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGa                                                                                                               <  2:3722260/149‑1 (MQ=255)
                                  aGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGAc                                                                                                              >  2:940334/1‑149 (MQ=255)
                                  aGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGAc                                                                                                              <  1:2788564/149‑1 (MQ=255)
                                        gTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTa                                                                                                        >  1:2966621/1‑149 (MQ=255)
                                         tCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTAc                                                                                                       >  2:2641840/1‑149 (MQ=255)
                                           ttGCTGCGTCGCCAATGGTGGCGCGTAACGCGCGTCTCCGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTa                                                                                                     <  1:2054543/149‑1 (MQ=255)
                                           ttGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTa                                                                                                     >  2:1733462/1‑149 (MQ=255)
                                           tggctgcGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTa                                                                                                     <  2:2252448/147‑1 (MQ=255)
                                            tgctgcGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAg                                                                                                    >  2:741765/1‑149 (MQ=255)
                                            tgctgcGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAg                                                                                                    <  1:2359649/149‑1 (MQ=255)
                                                        aaTGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCgtgg                                                                                        <  1:3768054/149‑1 (MQ=255)
                                                         aTGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCgtggt                                                                                       >  1:216895/1‑149 (MQ=255)
                                                              ggCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAg                                                                                  >  1:2689271/1‑149 (MQ=255)
                                                              ggCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAg                                                                                  >  2:196704/1‑149 (MQ=255)
                                                                cgcgTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGa                                                                                <  2:854746/149‑1 (MQ=255)
                                                                   gTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTTCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGt                                                                             >  1:941036/1‑149 (MQ=255)
                                                                   gTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGt                                                                             <  2:1404901/149‑1 (MQ=255)
                                                                   gTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGt                                                                             >  1:105107/1‑149 (MQ=255)
                                                                   gTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGt                                                                             <  1:1803222/149‑1 (MQ=255)
                                                                     aaCGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGc                                                                           >  2:2717084/1‑149 (MQ=255)
                                                                     aaCGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGc                                                                           >  2:1970517/1‑149 (MQ=255)
                                                                       cGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCa                                                                         >  2:1692026/1‑149 (MQ=255)
                                                                        gAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAt                                                                        <  2:431244/149‑1 (MQ=255)
                                                                        gAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAt                                                                        <  2:1679177/149‑1 (MQ=255)
                                                                            gTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTg                                                                    >  2:1542056/1‑149 (MQ=255)
                                                                              ctcAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGAt                                                                  <  2:1220863/149‑1 (MQ=255)
                                                                               tcAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGAtt                                                                 >  1:2988773/1‑149 (MQ=255)
                                                                                cAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTg                                                                <  1:594030/149‑1 (MQ=255)
                                                                                  gCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGc                                                              <  1:1028455/149‑1 (MQ=255)
                                                                                  gCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGc                                                              >  2:3410866/1‑149 (MQ=255)
                                                                                  gCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGc                                                              >  2:4086381/1‑149 (MQ=255)
                                                                                       tATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTaa                                                         >  1:940174/1‑149 (MQ=255)
                                                                                          gCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAAccc                                                      <  1:742886/149‑1 (MQ=255)
                                                                                             ggTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTgg                                                   <  1:490534/149‑1 (MQ=255)
                                                                                             ggTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTgg                                                   <  1:491447/149‑1 (MQ=255)
                                                                                              gTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGt                                                  <  2:3693776/149‑1 (MQ=255)
                                                                                                aGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGc                                                >  1:3398375/1‑149 (MQ=255)
                                                                                                aGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGc                                                <  1:1182938/149‑1 (MQ=255)
                                                                                                      ggggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                          >  1:2637230/1‑149 (MQ=255)
                                                                                                      ggggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                          >  2:3539416/1‑149 (MQ=255)
                                                                                                      ggggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                          >  2:3893802/1‑149 (MQ=255)
                                                                                                      ggggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaa                                          >  1:3412091/1‑149 (MQ=255)
                                                                                                       gggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaaa                                         <  2:797881/149‑1 (MQ=255)
                                                                                                       gggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGaaa                                         <  1:3731882/149‑1 (MQ=255)
                                                                                                        ggTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAAt                                        >  2:784381/1‑149 (MQ=255)
                                                                                                         gTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATc                                       <  1:3063103/149‑1 (MQ=255)
                                                                                                          tGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGCTCTGCCGCGTTTTTCCACCGGTTTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCTGGAAGTTCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATcc                                      <  1:2632341/149‑1 (MQ=255)
                                                                                                          tGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATcc                                      <  2:2345758/149‑1 (MQ=255)
                                                                                                           ggCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCa                                     <  1:363221/149‑1 (MQ=255)
                                                                                                              aaaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgc                                  >  2:3057888/1‑149 (MQ=255)
                                                                                                               aaaGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgct                                 >  2:3862252/1‑149 (MQ=255)
                                                                                                                aaGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgctg                                >  1:3530883/1‑149 (MQ=255)
                                                                                                                 aGTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgctgc                               <  2:4018587/149‑1 (MQ=255)
                                                                                                                  gTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgctgct                              >  1:3831194/1‑149 (MQ=255)
                                                                                                                  gTCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACgctgct                              >  2:1800458/1‑149 (MQ=255)
                                                                                                                   tCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCCCCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTa                             >  2:2138362/1‑149 (MQ=255)
                                                                                                                   tCCAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTa                             >  2:3749364/1‑149 (MQ=255)
                                                                                                                     cAGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACt                           >  1:96428/1‑149 (MQ=255)
                                                                                                                      aGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTg                          >  1:698412/1‑149 (MQ=255)
                                                                                                                      aGAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTg                          >  1:1942361/1‑149 (MQ=255)
                                                                                                                       gAAACTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGc                         >  2:1314266/1‑149 (MQ=255)
                                                                                                                          aCTCTCCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCAGTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCaaa                      <  2:520748/149‑1 (MQ=255)
                                                                                                                            tctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACg                    <  2:2217190/149‑1 (MQ=255)
                                                                                                                            tctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACg                    >  1:657111/1‑149 (MQ=255)
                                                                                                                            tctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACg                    <  2:141860/149‑1 (MQ=255)
                                                                                                                             ctcCGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGc                   >  2:1773207/1‑149 (MQ=255)
                                                                                                                                cGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtgt                <  1:400506/149‑1 (MQ=255)
                                                                                                                                cGATATCAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCtgt                >  1:1554811/1‑149 (MQ=255)
                                                                                                                                      cAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAAc          <  1:1705025/149‑1 (MQ=255)
                                                                                                                                      cAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAAc          <  1:1382678/149‑1 (MQ=255)
                                                                                                                                      cAGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAAc          <  2:3322540/149‑1 (MQ=255)
                                                                                                                                       aGCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACt         >  2:3283117/1‑149 (MQ=255)
                                                                                                                                        gCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTg        >  2:1968245/1‑149 (MQ=255)
                                                                                                                                        gCCTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGACGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTg        >  1:3766661/1‑149 (MQ=255)
                                                                                                                                         ccTTGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTgg       >  2:3988595/1‑149 (MQ=255)
                                                                                                                                            tGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGccc    <  2:3849257/149‑1 (MQ=255)
                                                                                                                                            tGAAGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGccc    <  2:1809881/149‑1 (MQ=255)
                                                                                                                                              aaGATCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCcag  <  2:2431453/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGGGAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAG  >  CP009273/4615801‑4616091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑