Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,617,341 C→A 100% F70L (TTC→TTA)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,617,3410CA99.5% 678.1 / ‑4.3 205F70L (TTC→TTA) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (126/78);  total (127/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

GCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGA  >  CP009273/4617195‑4617486
                                                                                                                                                  |                                                                                                                                                 
gCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAgg                                                                                                                                                 >  2:2394959/1‑149 (MQ=255)
gCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAgg                                                                                                                                                 >  1:2435087/1‑149 (MQ=255)
  tGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTa                                                                                                                                               <  1:1500159/149‑1 (MQ=255)
   gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTaa                                                                                                                                              <  2:4060350/149‑1 (MQ=255)
         aGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTa                                                                                                                                        <  1:3288752/149‑1 (MQ=255)
         aGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTa                                                                                                                                        >  1:2991604/1‑149 (MQ=255)
              tATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCAc                                                                                                                                   >  2:582979/1‑149 (MQ=255)
                tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAATCCACAGCGCGCAAAAGCTGGAGGCGATGCACCGTTTTTTTGGGCTTGAGTTTCCCCCGCAGAAGAAAACAATCGGTGACGTATTAGGTAACTTATACCCACtc                                                                                                                                 >  1:2979267/1‑148 (MQ=255)
                 gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGc                                                                                                                                >  2:802177/1‑149 (MQ=255)
                   ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTCAGTTCTACCCACTGCAt                                                                                                                              >  1:3701069/1‑149 (MQ=255)
                    cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATa                                                                                                                             >  2:1094292/1‑149 (MQ=255)
                    cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATa                                                                                                                             <  2:3367185/149‑1 (MQ=255)
                         ggTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGa                                                                                                                        >  2:143038/1‑149 (MQ=255)
                          gTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGAc                                                                                                                       >  2:1792565/1‑149 (MQ=255)
                           tATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACa                                                                                                                      <  2:1921148/149‑1 (MQ=255)
                           tATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACa                                                                                                                      >  2:3337970/1‑149 (MQ=255)
                               tAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATc                                                                                                                  <  1:372784/149‑1 (MQ=255)
                                  gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTAc                                                                                                               >  2:3342002/1‑149 (MQ=255)
                                   ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTAcc                                                                                                              <  2:1222765/149‑1 (MQ=255)
                                   ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTAcc                                                                                                              <  1:1275342/149‑1 (MQ=255)
                                    cAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAACAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCt                                                                                                             <  2:3224141/149‑1 (MQ=255)
                                      tttACTGATTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCTTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTa                                                                                                           <  1:3788021/148‑1 (MQ=255)
                                      gTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTa                                                                                                           <  2:3130492/149‑1 (MQ=255)
                                      gTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTa                                                                                                           <  2:1290096/149‑1 (MQ=255)
                                       ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTAt                                                                                                          >  1:2346122/1‑149 (MQ=255)
                                        tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATc                                                                                                         <  2:475548/149‑1 (MQ=255)
                                        tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATc                                                                                                         <  2:668643/149‑1 (MQ=255)
                                        tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATc                                                                                                         >  1:414183/1‑149 (MQ=255)
                                         aCTGAATGCCAAAATCCAAAGCCCCAGCGCGCAAAAGCTGGAGGCCGTGCACCGTGTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATcc                                                                                                        <  1:1704865/149‑1 (MQ=255)
                                         aCTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATcc                                                                                                        <  2:2403405/149‑1 (MQ=255)
                                         aCTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATcc                                                                                                        >  1:324488/1‑149 (MQ=255)
                                          cTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCa                                                                                                       >  2:780937/1‑149 (MQ=255)
                                          cTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCa                                                                                                       <  2:2241724/149‑1 (MQ=255)
                                          cTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCa                                                                                                       >  2:3858088/1‑149 (MQ=255)
                                           tGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAg                                                                                                      >  1:919988/1‑149 (MQ=255)
                                           tGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAg                                                                                                      >  2:2795014/1‑149 (MQ=255)
                                              aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcgc                                                                                                   >  1:2652191/1‑149 (MQ=255)
                                              aTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcgc                                                                                                   >  1:2481972/1‑149 (MQ=255)
                                                 ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGcc                                                                                                <  2:2019218/149‑1 (MQ=255)
                                                  cAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCt                                                                                               >  2:3206809/1‑149 (MQ=255)
                                                  cAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCt                                                                                               >  2:1624183/1‑149 (MQ=255)
                                                  cAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCt                                                                                               >  2:3435053/1‑149 (MQ=255)
                                                   aaaatcaaaatcCCCAGCGCGCAAACGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACCTATATACCTTCTACAGCGCGCCTg                                                                                              >  2:3965484/1‑149 (MQ=255)
                                                    aaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGt                                                                                             >  2:3367276/1‑149 (MQ=255)
                                                      aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAg                                                                                           >  1:1121668/1‑149 (MQ=255)
                                                      aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAg                                                                                           >  1:1814237/1‑149 (MQ=255)
                                                         aaaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCa                                                                                        >  1:2864948/1‑149 (MQ=255)
                                                          aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAg                                                                                       >  1:1023815/1‑149 (MQ=255)
                                                          aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAg                                                                                       <  1:438683/149‑1 (MQ=255)
                                                             gCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGtt                                                                                    >  2:1191194/1‑149 (MQ=255)
                                                             gCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGtt                                                                                    <  2:1805433/149‑1 (MQ=255)
                                                                  aGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGa                                                                               >  1:3549706/1‑149 (MQ=255)
                                                                   gcgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                                                                              >  2:1030302/1‑149 (MQ=255)
                                                                    cgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGc                                                                             <  1:873574/149‑1 (MQ=255)
                                                                    cgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGc                                                                             <  1:145877/149‑1 (MQ=255)
                                                                       gcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGc                                                                          <  2:2047146/149‑1 (MQ=255)
                                                                         aaaaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATCGGTGTAGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCAGTAGCCAGGTTGACGAGCTGCat                                                                        >  1:25309/1‑149 (MQ=255)
                                                                          aaaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCata                                                                       >  1:2774950/1‑149 (MQ=255)
                                                                           aaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCatat                                                                      <  1:70648/149‑1 (MQ=255)
                                                                              cTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCAt                                                                   >  1:2021324/1‑149 (MQ=255)
                                                                                 gAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAt                                                                >  1:911815/1‑149 (MQ=255)
                                                                                   ggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATgg                                                              >  1:2379346/1‑149 (MQ=255)
                                                                                   ggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATgg                                                              >  1:3195404/1‑149 (MQ=255)
                                                                                   ggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATgg                                                              >  1:3199313/1‑149 (MQ=255)
                                                                                      gTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                           >  1:841172/1‑149 (MQ=255)
                                                                                      gTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                           >  2:3698854/1‑149 (MQ=255)
                                                                                       ttGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt                                                          <  1:572457/149‑1 (MQ=255)
                                                                                       ttGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt                                                          <  1:572601/149‑1 (MQ=255)
                                                                                       ttGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCAGATCATTATGGGttt                                                          >  2:3788567/1‑149 (MQ=255)
                                                                                         gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTg                                                        >  2:1892625/1‑149 (MQ=255)
                                                                                         gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTg                                                        >  2:2374682/1‑149 (MQ=255)
                                                                                           aCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGAc                                                      <  1:336060/149‑1 (MQ=255)
                                                                                             cGTTTTTTGGGGCTTGAGTGTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTGGACGa                                                    <  2:1465806/149‑1 (MQ=255)
                                                                                                  tttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATAccc                                               <  2:1389114/149‑1 (MQ=255)
                                                                                                    tGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGt                                             <  1:3592727/149‑1 (MQ=255)
                                                                                                     ggggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTCTGGGTTTTGACGATACCCGTg                                            >  2:1407615/1‑149 (MQ=255)
                                                                                                      gggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGa                                           >  1:2852318/1‑149 (MQ=255)
                                                                                                          ttGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgc                                       >  2:2094372/1‑149 (MQ=255)
                                                                                                            gAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgc                                     >  2:2257541/1‑149 (MQ=255)
                                                                                                                  ccccGGCAGAAGAAAACGATCGGTGTCGAATTAGGTAAGTTATACCCACTGCATAACGGACAAATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttgtt                               >  1:923673/1‑149 (MQ=255)
                                                                                                                      ggCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCGACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGaa                           <  2:2539140/149‑1 (MQ=255)
                                                                                                                       gCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAg                          <  1:1329455/149‑1 (MQ=255)
                                                                                                                        cAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGa                         >  1:2502096/1‑149 (MQ=255)
                                                                                                                         agaagaAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc                        >  1:2285999/1‑149 (MQ=255)
                                                                                                                          gaagaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATGATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACa                       >  1:4033022/1‑149 (MQ=255)
                                                                                                                            agaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGt                     >  1:1305132/1‑149 (MQ=255)
                                                                                                                             gaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTg                    >  2:2953759/1‑149 (MQ=255)
                                                                                                                               aaaCGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGcc                  >  2:3499671/1‑149 (MQ=255)
                                                                                                                                 aCGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt                >  1:1491463/1‑149 (MQ=255)
                                                                                                                                     tCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCg            >  1:1410734/1‑149 (MQ=255)
                                                                                                                                     tCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCg            >  1:1410062/1‑149 (MQ=255)
                                                                                                                                       ggTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgca          <  2:2435087/149‑1 (MQ=255)
                                                                                                                                        gtgtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcag         <  2:2739834/149‑1 (MQ=255)
                                                                                                                                        gtgtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcag         >  1:2754851/1‑149 (MQ=255)
                                                                                                                                          gtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACCTATCTACCTTATCCAGCGCGCCTGTAGCCAGGTAGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagca       >  2:3753378/1‑149 (MQ=255)
                                                                                                                                           tCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGcagcag      <  1:3363529/149‑1 (MQ=255)
                                                                                                                                            cGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCagcagc     <  1:799812/149‑1 (MQ=255)
                                                                                                                                             gTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGcc    <  2:2687553/149‑1 (MQ=255)
                                                                                                                                               aTTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGa  >  2:2123181/1‑149 (MQ=255)
                                                                                                                                               aTTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGa  >  2:2740339/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGA  >  CP009273/4617195‑4617486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑