Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 432,762 G→T 100% P277Q (CCG→CAG)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273432,7620GT96.7% 736.7 / 13.7 240P277Q (CCG→CAG) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base T (137/95);  total (143/97)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

CTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCG  >  CP009273/432616‑432907
                                                                                                                                                  |                                                                                                                                                 
 ttATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTTTTCCGGCTTCAAAGTGATATCAACCGCGTTCAATACCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGtt                                                                                                                                                >  1:3125035/1‑149 (MQ=255)
 ttATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGtt                                                                                                                                                >  2:3655598/1‑149 (MQ=255)
  tATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGttt                                                                                                                                               >  2:2617318/1‑149 (MQ=255)
    tttAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTAc                                                                                                                                             >  2:294739/1‑149 (MQ=255)
    tttAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTAc                                                                                                                                             <  1:2301217/149‑1 (MQ=255)
      tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTc                                                                                                                                           >  1:3418380/1‑149 (MQ=255)
      tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTc                                                                                                                                           <  2:422920/149‑1 (MQ=255)
      tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTc                                                                                                                                           <  2:38393/149‑1 (MQ=255)
      tAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTc                                                                                                                                           <  1:2007975/149‑1 (MQ=255)
           ccTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaacaa                                                                                                                                      >  2:3827631/1‑149 (MQ=255)
            cTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                     >  2:3311709/1‑149 (MQ=255)
                    ggATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAg                                                                                                                             >  1:1854118/1‑149 (MQ=255)
                            tttATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACtt                                                                                                                     <  2:3155945/149‑1 (MQ=255)
                               ataCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTtgtg                                                                                                                  <  1:1692191/149‑1 (MQ=255)
                                taCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGc                                                                                                                 >  2:3982772/1‑149 (MQ=255)
                                  cGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCtn                                                                                                               <  1:2652012/149‑2 (MQ=255)
                                  cGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTc                                                                                                               >  2:4066370/1‑149 (MQ=255)
                                  cGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTc                                                                                                               >  2:760922/1‑149 (MQ=255)
                                    gCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgt                                                                                                             >  2:3038877/1‑149 (MQ=255)
                                    gCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgt                                                                                                             >  2:2760473/1‑149 (MQ=255)
                                     cGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtg                                                                                                            <  2:1852499/149‑1 (MQ=255)
                                      gTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                           >  1:2424065/1‑149 (MQ=255)
                                       tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTc                                                                                                          >  1:1766534/1‑149 (MQ=255)
                                           cAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccc                                                                                                      >  1:920301/1‑149 (MQ=255)
                                           cAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccc                                                                                                      >  2:905955/1‑149 (MQ=255)
                                                 ggCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGtt                                                                                                <  1:2259705/149‑1 (MQ=255)
                                                 ggCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGtt                                                                                                >  1:999015/1‑149 (MQ=255)
                                                    aaTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                             >  1:1065872/1‑149 (MQ=255)
                                                    aaTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                             <  1:4099905/149‑1 (MQ=255)
                                                     aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttcttc                                                                                            >  2:3663786/1‑149 (MQ=255)
                                                      tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                           >  1:3578896/1‑149 (MQ=255)
                                                      tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                           >  1:360101/1‑149 (MQ=255)
                                                      tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                           <  1:3708/149‑1 (MQ=255)
                                                      tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                           >  1:3744017/1‑149 (MQ=255)
                                                        tGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACt                                                                                         >  2:3747336/1‑149 (MQ=255)
                                                               gCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt                                                                                  >  2:1445952/1‑149 (MQ=255)
                                                               gCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt                                                                                  >  2:1012485/1‑149 (MQ=255)
                                                                 ttCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGt                                                                                >  1:262678/1‑149 (MQ=255)
                                                                  tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGtt                                                                               >  2:50079/1‑149 (MQ=255)
                                                                  tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGACAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGtt                                                                               >  2:3888171/1‑149 (MQ=255)
                                                                    aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTaa                                                                             >  1:2091877/1‑149 (MQ=255)
                                                                     aaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                            >  1:3469186/1‑149 (MQ=255)
                                                                     aaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                            >  1:3955735/1‑149 (MQ=255)
                                                                      aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc                                                                           >  2:1171102/1‑149 (MQ=255)
                                                                      aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc                                                                           <  1:305276/149‑1 (MQ=255)
                                                                      aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc                                                                           >  1:1226688/1‑149 (MQ=255)
                                                                          atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctc                                                                       <  2:2412145/149‑1 (MQ=255)
                                                                          atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctc                                                                       >  2:595044/1‑149 (MQ=255)
                                                                           tatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctct                                                                      >  1:1328683/1‑149 (MQ=255)
                                                                           tatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctct                                                                      >  1:1089506/1‑149 (MQ=255)
                                                                           tatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGctct                                                                      >  1:3233747/1‑149 (MQ=255)
                                                                             tCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGc                                                                    <  1:1296283/149‑1 (MQ=255)
                                                                                aCCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGAt                                                                 >  2:1026030/1‑149 (MQ=255)
                                                                                 ccGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATc                                                                >  2:1330477/1‑149 (MQ=255)
                                                                                      ttCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                           >  2:1652078/1‑149 (MQ=255)
                                                                                      ttCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                           >  2:1775690/1‑149 (MQ=255)
                                                                                      ttCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                           >  2:619998/1‑149 (MQ=255)
                                                                                        cAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGt                                                         <  2:2368444/149‑1 (MQ=255)
                                                                                        cAAGAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGt                                                         <  2:2437438/149‑1 (MQ=255)
                                                                                         aaTAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTc                                                        >  2:2519833/1‑149 (MQ=255)
                                                                                           tAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAt                                                      <  2:1558629/149‑1 (MQ=255)
                                                                                            aGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                     >  2:2025272/1‑149 (MQ=255)
                                                                                            aGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtt                                                     <  1:447573/149‑1 (MQ=255)
                                                                                             gCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAttt                                                    <  1:3443877/149‑1 (MQ=255)
                                                                                              cTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTTCTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAtttt                                                   <  2:3426954/149‑1 (MQ=255)
                                                                                                catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                 <  2:2330469/149‑1 (MQ=255)
                                                                                                catcaAGCTGTTCTTCGCGCGAAGGTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                 <  2:1879169/149‑1 (MQ=255)
                                                                                                  tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATc                                               >  2:2583678/1‑149 (MQ=255)
                                                                                                     aGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                            >  1:32618/1‑149 (MQ=255)
                                                                                                     aGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                            >  1:3680501/1‑149 (MQ=255)
                                                                                                     aGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                            >  2:1515562/1‑149 (MQ=255)
                                                                                                      gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctt                                           >  2:701073/1‑149 (MQ=255)
                                                                                                         gTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                        <  1:3310403/149‑1 (MQ=255)
                                                                                                             ttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtat                                    <  2:3731404/149‑1 (MQ=255)
                                                                                                              tcGCGCGAAGTTCCGATCATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtata                                   <  2:3929438/149‑1 (MQ=255)
                                                                                                                gcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATaga                                 >  1:2380118/1‑149 (MQ=255)
                                                                                                                 cgcgAAGTTCCGATAATCGGTGCGGTAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATagag                                <  2:2510786/149‑1 (MQ=255)
                                                                                                                  gcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAgaga                               <  1:3827631/149‑1 (MQ=255)
                                                                                                                  gcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATGTTCATCGCTTTCTTTATAgaga                               >  1:2502264/1‑149 (MQ=255)
                                                                                                                      aGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAtttt                           >  2:437811/1‑149 (MQ=255)
                                                                                                                       gTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttttt                          >  1:2888102/1‑149 (MQ=255)
                                                                                                                       gTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttttt                          <  2:2166832/149‑1 (MQ=255)
                                                                                                                        ttCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTGTATAGAGATTTTTc                         >  2:2620297/1‑149 (MQ=255)
                                                                                                                         tCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCGTTATAGAGATTTTTcc                        >  2:507027/1‑149 (MQ=255)
                                                                                                                          ccGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTccc                       <  1:1025721/149‑1 (MQ=255)
                                                                                                                           cGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcccc                      >  1:679549/1‑149 (MQ=255)
                                                                                                                           cGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcccc                      >  1:1381379/1‑149 (MQ=255)
                                                                                                                           cGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcccc                      <  2:3807485/149‑1 (MQ=255)
                                                                                                                            gATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCa                     >  1:803244/1‑149 (MQ=255)
                                                                                                                              tAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCAcc                   <  1:2580257/149‑1 (MQ=255)
                                                                                                                                aTCGGTGTGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                 <  1:1654899/149‑1 (MQ=255)
                                                                                                                                aTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                 <  2:3578896/149‑1 (MQ=255)
                                                                                                                                        ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGaca         <  2:3381920/149‑1 (MQ=255)
                                                                                                                                           aaTGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCa      >  1:2882647/1‑149 (MQ=255)
                                                                                                                                           aaTGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCa      >  1:2130520/1‑149 (MQ=255)
                                                                                                                                           aaTGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCa      >  2:2843097/1‑149 (MQ=255)
                                                                                                                                              gCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTc   >  2:4032907/1‑149 (MQ=255)
                                                                                                                                               cccTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCg  <  1:1495473/149‑1 (MQ=255)
                                                                                                                                               cccTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCg  >  2:1918078/1‑149 (MQ=255)
                                                                                                                                               cccTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCg  >  1:3815446/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCG  >  CP009273/432616‑432907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑