Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 446,369 T→G 100% Q233P (CAG→CCG)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273446,3690TG98.8% 1069.2 / ‑2.4 266Q233P (CAG→CCG) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base G (143/119);  total (147/119)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

TAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGC  >  CP009273/446223‑446514
                                                                                                                                                  |                                                                                                                                                 
  aaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTc                                                                                                                                               >  2:1701290/1‑149 (MQ=255)
  aaCTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTc                                                                                                                                               >  2:2222057/1‑149 (MQ=255)
     ttGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                            <  2:1844307/149‑1 (MQ=255)
      tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAAt                                                                                                                                           <  1:2711294/149‑1 (MQ=255)
         tAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcg                                                                                                                                        <  1:125675/149‑1 (MQ=255)
         tAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcg                                                                                                                                        >  2:2002505/1‑149 (MQ=255)
         tAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcg                                                                                                                                        >  1:2020328/1‑149 (MQ=255)
           aTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggc                                                                                                                                      >  1:1517575/1‑149 (MQ=255)
           aTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggc                                                                                                                                      >  2:2544298/1‑149 (MQ=255)
                aTCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGAt                                                                                                                                 >  2:655182/1‑149 (MQ=255)
                 tCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                                <  2:992364/149‑1 (MQ=255)
                 tCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                                <  1:473931/149‑1 (MQ=255)
                    gCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCgg                                                                                                                             >  2:685993/1‑149 (MQ=255)
                    gCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGGTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCgg                                                                                                                             >  2:1022230/1‑149 (MQ=255)
                      aaaCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTATCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtg                                                                                                                           <  2:1113196/149‑1 (MQ=255)
                        aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGtt                                                                                                                         <  2:3024424/149‑1 (MQ=255)
                           aGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCa                                                                                                                      >  1:1556488/1‑149 (MQ=255)
                            gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCaa                                                                                                                     <  2:318711/149‑1 (MQ=255)
                              cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATa                                                                                                                   <  1:903877/149‑1 (MQ=255)
                                ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGc                                                                                                                 <  2:1250738/149‑1 (MQ=255)
                                ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGc                                                                                                                 >  1:601172/1‑149 (MQ=255)
                                 gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCt                                                                                                                >  2:3691442/1‑149 (MQ=255)
                                 gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCt                                                                                                                <  1:4043753/149‑1 (MQ=255)
                                   ttCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCttt                                                                                                              <  2:1932731/149‑1 (MQ=255)
                                      aCGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGaa                                                                                                           >  1:3423754/1‑149 (MQ=255)
                                         ttGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                        >  2:1735874/1‑149 (MQ=255)
                                         ttGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                        >  1:306803/1‑149 (MQ=255)
                                           ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCa                                                                                                      >  2:1668614/1‑149 (MQ=255)
                                           ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTATGAACTTCa                                                                                                      >  1:2396137/1‑149 (MQ=255)
                                            gagaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCAt                                                                                                     <  1:3318025/149‑1 (MQ=255)
                                            gagaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCAt                                                                                                     >  2:154792/1‑149 (MQ=255)
                                             agaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATg                                                                                                    >  1:2097031/1‑149 (MQ=255)
                                              gaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGc                                                                                                   >  2:2082092/1‑149 (MQ=255)
                                               aaaTATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGcc                                                                                                  >  2:3006837/1‑149 (MQ=255)
                                               aaaTATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGcc                                                                                                  <  2:2642698/149‑1 (MQ=255)
                                                aaTATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCt                                                                                                 >  2:2227727/1‑149 (MQ=255)
                                                 atatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTg                                                                                                <  1:3120097/149‑1 (MQ=255)
                                                 atatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTg                                                                                                >  2:4050029/1‑149 (MQ=255)
                                                  tatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                               >  2:791445/1‑149 (MQ=255)
                                                  tatTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                               <  2:3322660/149‑1 (MQ=255)
                                                    ttCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                             >  1:1921829/1‑149 (MQ=255)
                                                    ttCCACCTGGTTGTATTCGCTAGGCGCAGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                             >  1:2922348/1‑149 (MQ=255)
                                                     tCCACCTGGTTGTATTCGCTAGGCGCGTCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTCGCAATAGCTTTGAACTTCATGCCTGAGaa                                                                                            <  2:3574852/149‑1 (MQ=255)
                                                      ccACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                           <  1:3991993/149‑1 (MQ=255)
                                                       cACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGc                                                                                          <  1:950796/149‑1 (MQ=255)
                                                        aCCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATATCTTTGAACTTCATGCCTGAGAAGcc                                                                                         <  2:1840342/149‑1 (MQ=255)
                                                        aCCTGGTTGTATTCGCGAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACAGGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGcc                                                                                         <  2:2126237/149‑1 (MQ=255)
                                                         ccTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGccc                                                                                        <  1:3744790/149‑1 (MQ=255)
                                                          cttgTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACAGGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCg                                                                                       <  2:521849/146‑1 (MQ=255)
                                                          cTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCg                                                                                       <  2:3537904/149‑1 (MQ=255)
                                                            ggTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCggg                                                                                     >  1:2271103/1‑149 (MQ=255)
                                                             gTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                    >  1:3561836/1‑149 (MQ=255)
                                                                gTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGc                                                                                 <  1:2813176/149‑1 (MQ=255)
                                                                  aTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTg                                                                               >  1:2734985/1‑149 (MQ=255)
                                                                  aTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTg                                                                               >  1:2827260/1‑149 (MQ=255)
                                                                  aTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTg                                                                               >  1:2440748/1‑149 (MQ=255)
                                                                   ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGt                                                                              <  2:2742873/149‑1 (MQ=255)
                                                                       cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCt                                                                          <  2:939026/149‑1 (MQ=255)
                                                                         aGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCGGTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTgg                                                                        >  2:2469253/1‑149 (MQ=255)
                                                                           gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCg                                                                      >  2:1451597/1‑149 (MQ=255)
                                                                            cgcgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCgg                                                                     <  1:1466698/149‑1 (MQ=255)
                                                                                   aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAcc                                                              >  1:301633/1‑149 (MQ=255)
                                                                                   aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAcc                                                              <  2:346953/149‑1 (MQ=255)
                                                                                     tttttCGAACGCAGCCGTGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGt                                                            <  2:2402965/149‑1 (MQ=255)
                                                                                       tttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCa                                                          <  1:1230205/149‑1 (MQ=255)
                                                                                       tttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCa                                                          >  2:2182668/1‑149 (MQ=255)
                                                                                        ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCAt                                                         <  1:285479/149‑1 (MQ=255)
                                                                                          cGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATaa                                                       <  1:1306465/149‑1 (MQ=255)
                                                                                           gAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAg                                                      <  1:2467315/149‑1 (MQ=255)
                                                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                     <  2:3863407/149‑1 (MQ=255)
                                                                                            aaCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                     <  2:3783196/149‑1 (MQ=255)
                                                                                             aCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTg                                                    >  1:3548605/1‑149 (MQ=255)
                                                                                                cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGTGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                 >  2:3656259/1‑149 (MQ=255)
                                                                                                cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGTGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCg                                                 >  2:3654527/1‑149 (MQ=255)
                                                                                                  gCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCggg                                               <  2:2407252/149‑1 (MQ=255)
                                                                                                   ccATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGt                                              <  2:1035500/149‑1 (MQ=255)
                                                                                                       gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                          >  1:1788728/1‑149 (MQ=255)
                                                                                                        tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGtt                                         >  2:431397/1‑149 (MQ=255)
                                                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                       <  2:108540/149‑1 (MQ=255)
                                                                                                          aGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                       <  1:2700698/149‑1 (MQ=255)
                                                                                                           gACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGc                                      <  1:3226340/149‑1 (MQ=255)
                                                                                                                  tgtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGa                               <  1:1672442/149‑1 (MQ=255)
                                                                                                                   gtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGAt                              >  1:76681/1‑149 (MQ=255)
                                                                                                                     tCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGc                            <  2:3932633/149‑1 (MQ=255)
                                                                                                                         cGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGc                        <  2:2834780/149‑1 (MQ=255)
                                                                                                                          gccTGCTTCGCTTTTGCGACCCCCGGGTCGACTGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACGTCATGCCTGAGAAGCCCGGGCCGCTGGAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCg                       <  1:2222057/147‑1 (MQ=255)
                                                                                                                          gACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCg                       >  1:2618732/1‑149 (MQ=255)
                                                                                                                           aCTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGa                      <  1:2544298/149‑1 (MQ=255)
                                                                                                                           aCTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGa                      >  1:965011/1‑149 (MQ=255)
                                                                                                                            cTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                     <  1:3567684/149‑1 (MQ=255)
                                                                                                                                ttCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTg                 >  1:1105082/1‑149 (MQ=255)
                                                                                                                                 tCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGc                >  1:1894629/1‑149 (MQ=255)
                                                                                                                                 tCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGc                <  2:2827116/149‑1 (MQ=255)
                                                                                                                                 tCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGc                <  2:741167/149‑1 (MQ=255)
                                                                                                                                  cGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCa               <  2:849263/149‑1 (MQ=255)
                                                                                                                                   gCTTTTGCGACCCACTGGTCGAATGAGGCGCGATCCGGTGTTGCAATATCTTTGAACTTCATGACTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGACTGCAt              >  1:2348464/1‑149 (MQ=255)
                                                                                                                                   gCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCTGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt              <  2:156075/149‑1 (MQ=255)
                                                                                                                                       ttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCg          >  2:2671626/1‑149 (MQ=255)
                                                                                                                                         gCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGc        <  1:1275511/149‑1 (MQ=255)
                                                                                                                                               cACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGc  >  1:657367/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGC  >  CP009273/446223‑446514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑