Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,333 A→T 8.8% intergenic (+88/‑256) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,3330AT 8.8% 615.1 / 2.8 249intergenic (+88/‑256)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base A (77/137);  new base T (15/3);  total (109/140)
Fisher's exact test for biased strand distribution p-value = 1.79e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450185‑450481
                                                                                                                                                    |                                                                                                                                                       
   gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt‑tt                                                                                                                                                     <  1:97868/149‑1 (MQ=255)
   gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt‑tt                                                                                                                                                     <  1:97792/149‑1 (MQ=255)
   gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt‑tt                                                                                                                                                     <  1:2887539/149‑1 (MQ=255)
      gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTgc                                                                                                                                                  <  1:1179122/149‑1 (MQ=255)
            cacCGTCTGTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCtttt                                                                                                                                            <  2:3829159/149‑1 (MQ=255)
              ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTg                                                                                                                                          <  2:1373927/149‑1 (MQ=255)
                 tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTGTGGGTGTTTGGGTTTTTTGcc                                                                                                                                        >  1:2479953/1‑149 (MQ=255)
                  cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCa                                                                                                                                      <  2:1507982/149‑1 (MQ=255)
                      gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCAt                                                                                                                                  <  2:1268711/149‑1 (MQ=255)
                         tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCa                                                                                                                               <  2:83124/149‑1 (MQ=255)
                         tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCa                                                                                                                               <  2:82563/149‑1 (MQ=255)
                            cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGAT‑TTTTGGGTTTTTGCCCCGCCTTCAGA‑c                                                                                                                           >  2:3483034/1‑149 (MQ=255)
                                 cTGTAGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑aaaa                                                                                                                     <  2:3274393/149‑1 (MQ=255)
                                  tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAt                                                                                                                    <  1:888445/149‑1 (MQ=255)
                                   gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAtt                                                                                                                   <  1:3931681/149‑1 (MQ=255)
                                   gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAtt                                                                                                                   <  1:255649/149‑1 (MQ=255)
                                    tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTg                                                                                                                  >  2:3680787/1‑149 (MQ=255)
                                      gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGcc                                                                                                                <  2:729747/149‑1 (MQ=255)
                                       tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTTGGT‑TTTTGGGTTTTTTTCCAGCATTGAGA‑AGCAAAAttaac                                                                                                                >  1:3251390/1‑146 (MQ=255)
                                         gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCgg                                                                                                             <  1:673824/149‑1 (MQ=255)
                                           gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGGTTTTTTTGTGGTG‑TTTTAGGTTTTTTGCCAGCACTCAGA‑CG‑AAAAATGCCCcggg                                                                                                           >  1:2305106/1‑148 (MQ=255)
                                            cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGaa                                                                                                          <  1:1859871/149‑1 (MQ=255)
                                             aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTTTGTGGGT‑TTTGGGCTTTTTGCCCCGCAGTCAGA‑CG‑AAAAGTGGCCgggcag                                                                                                         >  2:1181942/1‑146 (MQ=255)
                                              ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGGT‑TTTGGGGTTTTTTGCCAGCAATCAGT‑CG‑AAAATTTCCCGGGGAtt                                                                                                        >  1:2025095/1‑149 (MQ=255)
                                              ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTTTGGTT‑TTTTGGGTTTTTTTCCAGCAATAATA‑CC‑TAAAATTCCCggggagt                                                                                                        >  1:118524/1‑145 (MQ=255)
                                              ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTGTGGGT‑TTTGGGGTTTTTGGCCCGCCTTCAGAGCA‑AAAAATGCCGGGGCAt                                                                                                         >  1:791414/1‑149 (MQ=255)
                                               gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGGT‑TTTGGGCTTTTTGGCCAGCATTCAGA‑CG‑AAAATTGCCCGGGGAttt                                                                                                       >  2:386396/1‑148 (MQ=255)
                                                aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATtgt                                                                                                      <  2:611706/149‑1 (MQ=255)
                                                aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATtgt                                                                                                      <  1:4106531/149‑1 (MQ=255)
                                                aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGGGGGT‑TTTTGGGTTTTTGGCCAGCCTTAAGT‑CG‑AAAATTTGCCGGGGAtttt                                                                                                      >  1:4078640/1‑147 (MQ=255)
                                                aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTTTTTTGTGTGGGT‑TTTGGGGTTTTTGGCCCCCCATAAGA‑CGAAAAATGGCCCgggcgttt                                                                                                       >  1:2528763/1‑144 (MQ=255)
                                                 aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTTTGGTT‑TTTTTGGTTTTTTGCCCGCAATAAGACCA‑AAAATTGGCCGGG‑ATtgtg                                                                                                     >  1:2960173/1‑149 (MQ=255)
                                                 aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATtgtg                                                                                                     >  2:3020140/1‑149 (MQ=255)
                                                 aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTCTGGAG‑TTGGAGCTTTTTGGCCAGCATTCAGA‑CG‑AAAATTGGCCgggccatgtt                                                                                                     >  2:630128/1‑142 (MQ=255)
                                                  gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGa                                                                                                    <  1:3722418/149‑1 (MQ=255)
                                                   cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGaa                                                                                                   <  2:174793/149‑1 (MQ=255)
                                                   cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTTTGTGGGT‑TTTTGGCTTTTTTGCCACCATTAAGA‑AG‑AAAATTGGCCGGGGATtgtgta                                                                                                   >  2:1795341/1‑147 (MQ=255)
                                                    aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGGGCTTTTTGCCCAGCAATCAGA‑CG‑AAAATTGCCCGGGTCTTGTGtaa                                                                                                  >  2:3932109/1‑149 (MQ=255)
                                                         gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATa                                                                                             <  2:3655029/149‑1 (MQ=255)
                                                            aaaaCGCATGTGCAACTGTCGGCGCTTTACCAGTATGTTGCAAAAAATTTTATGACAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                                          <  1:3792969/149‑1 (MQ=255)
                                                            aaaaAGAATTTGCAACTGTCGGCGCTTTTCATGTATTTTTATAAAAATTTTATGAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                                          <  1:3793891/149‑1 (MQ=255)
                                                              aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTTTGGTT‑TTTTGGGTTTTTTGCCAGCATTGAGA‑CG‑CAAATTTCCCGGGGATTGTGTAAAAAAAagcgg                                                                                        >  1:2621578/1‑148 (MQ=255)
                                                              aaCGAATTTTAACCTGTGGGCGATTTACCAGTATGTTTCTAAAATTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                                                                        <  1:1744097/149‑1 (MQ=255)
                                                                        cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                                                              <  2:916960/149‑1 (MQ=255)
                                                                         aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGAT‑TTTTCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAATGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCCAt                                                                             >  2:4047917/1‑149 (MQ=255)
                                                                           cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                           <  2:1704778/149‑1 (MQ=255)
                                                                             gTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACAGCCTGCGGGCCGTTTTGTTTTTTCTGGAT‑TTTTCGCTTTTTTCCCACCAATAAGA‑CG‑AAAAATTCCCCGGAAATTTGAAAAAATAACCGACAGAGCGCAATAAcc                                                                         >  2:1581970/1‑149 (MQ=255)
                                                                                 gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGAT‑TTTTCGCTTTTTGCCCCCCATTCATA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAAAAACCGTtc                                                                     >  2:124990/1‑149 (MQ=255)
                                                                                 gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                                                                     >  2:2038203/1‑149 (MQ=255)
                                                                                  cgcTTTCCCATTTTGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                                    <  1:2905838/149‑1 (MQ=255)
                                                                                   gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                   <  2:2196243/149‑1 (MQ=255)
                                                                                        tCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                              <  1:3028893/149‑1 (MQ=255)
                                                                                          cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGGTTTTTGCCCAGCATTAAGA‑CG‑AAAATTGCCCGGGCCTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                            >  2:3951521/1‑149 (MQ=255)
                                                                                              aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGAT‑TTTTCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTTCCCGGGAAATTTTAAAAAATACGCGACAGCGCGCAATAAACGTTAAAGACTCaaaaaa                                                        >  2:2393609/1‑149 (MQ=255)
                                                                                              aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                        <  2:1838784/149‑1 (MQ=255)
                                                                                              aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                        <  1:2248397/149‑1 (MQ=255)
                                                                                              aTGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                        <  1:2185409/149‑1 (MQ=255)
                                                                                                tttgaaaaaaATTTTATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGAATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                      <  1:940793/143‑1 (MQ=255)
                                                                                                 ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTTTTGAT‑TTTTGGGTTTTTTCCCAGCAATAAGA‑CG‑AAAAATTCCCCGGGCTTTTTAAAAAATAAGCGACAGCGCGCAAAAAACCTTATAGAATCaaaaaaatt                                                     >  1:1004504/1‑146 (MQ=255)
                                                                                                     tAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                 <  1:2405927/149‑1 (MQ=255)
                                                                                                      aaaaATTTTATGAAAAACGGCCTGCGGGCCTTTTTGTTCTGTCTGTAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                                <  1:1801347/149‑1 (MQ=255)
                                                                                                        aaaTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                                              <  1:1321892/147‑1 (MQ=255)
                                                                                                        aaaTTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTTCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGTTATCGACTCATAAAAGTTATGCCGc                                              >  2:3550257/3‑149 (MQ=255)
                                                                                                        aaaTTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGAT‑TTTTCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAATGCCCGGGCAATGTGAAAAAAAAAGCGACAGCGCGCAATAACCGGTCTCGACTCATAAAAATGATGCCGc                                              >  2:1500976/3‑149 (MQ=255)
                                                                                                         aaTTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGAT‑TTTGGGCTTTTTTCCCACCATTCACA‑AG‑AAAAATTCCCGGGCATTTTGAAAAAATAAGCGCCACCGCGCCATAAACCTTCTCGAAGCCAAAAAGTTATTCCGcc                                             >  1:2688752/2‑148 (MQ=255)
                                                                                                         aaTTTTAAGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCt                                             <  2:2865570/148‑1 (MQ=255)
                                                                                                             ttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATaa                                         <  1:2899954/149‑1 (MQ=255)
                                                                                                              tATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                                        <  1:3248644/149‑1 (MQ=255)
                                                                                                                  aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCAATCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATTCCGCTATAATGCcg                                    >  2:2749230/1‑149 (MQ=255)
                                                                                                                       cGGCCTGCGGGCCGTTTTGTTGTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                               <  2:2479940/149‑1 (MQ=255)
                                                                                                                         gCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTTCGCATTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGCATTGTGAAAAAATAAGCGACAGCGCGCAATAACCCTTCTAGACTCAAAAAAGTTATGCCCCAATAAAGACGCGTACTa                             >  2:4086607/1‑149 (MQ=255)
                                                                                                                               gggCCGTTTTGTTTTTTCTGGAT‑TTTGCGCTTTTTGCCCATCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                       <  2:1955948/149‑1 (MQ=255)
                                                                                                                               gggCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                       <  1:2812934/149‑1 (MQ=255)
                                                                                                                                    gttttgttttGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                  >  1:402630/1‑149 (MQ=255)
                                                                                                                                     ttttgttttgTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                 <  2:3091276/149‑1 (MQ=255)
                                                                                                                                      tttgtttggtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                <  1:3063009/149‑1 (MQ=255)
                                                                                                                                       ttgttttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCg               <  1:2049723/149‑1 (MQ=255)
                                                                                                                                        tgttttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg              >  2:44813/1‑149 (MQ=255)
                                                                                                                                          ttttgtCTGTAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGTATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            <  2:3075995/149‑1 (MQ=255)
                                                                                                                                          ttttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            >  1:2650785/1‑149 (MQ=255)
                                                                                                                                           tttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat           <  2:2800346/149‑1 (MQ=255)
                                                                                                                                           tttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat           <  2:2832917/149‑1 (MQ=255)
                                                                                                                                            ttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg          <  1:758876/149‑1 (MQ=255)
                                                                                                                                            ttgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg          >  1:627160/1‑149 (MQ=255)
                                                                                                                                             tgtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatga         <  2:2879324/149‑1 (MQ=255)
                                                                                                                                              gtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat        <  2:1553419/149‑1 (MQ=255)
                                                                                                                                              gtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat        <  2:1208400/149‑1 (MQ=255)
                                                                                                                                              gtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat        >  2:2597251/1‑149 (MQ=255)
                                                                                                                                              gtCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat        >  2:3884979/1‑149 (MQ=255)
                                                                                                                                               tCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       <  2:2207420/149‑1 (MQ=255)
                                                                                                                                                cTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc      <  1:2474358/149‑1 (MQ=255)
                                                                                                                                                 tGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt     <  2:3304028/149‑1 (MQ=255)
                                                                                                                                                 tGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt     <  2:1430662/149‑1 (MQ=255)
                                                                                                                                                 tGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt     >  2:1448239/1‑149 (MQ=255)
                                                                                                                                                  ggAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg    <  1:930473/149‑1 (MQ=255)
                                                                                                                                                  ggAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg    >  1:1521599/1‑149 (MQ=255)
                                                                                                                                                   gAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTgg   >  2:2109882/1‑149 (MQ=255)
                                                                                                                                                   gAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTgg   >  2:2956546/1‑149 (MQ=255)
                                                                                                                                                    aT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt  >  1:3586186/1‑149 (MQ=255)
                                                                                                                                                    aT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt  >  2:2947435/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT‑TTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450185‑450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑