Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,499 G→T 100% R424S (CGC→AGC)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,4990GT98.8% 890.7 / 0.4 278R424S (CGC→AGC) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (142/132);  total (145/133)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACT  >  CP009273/461354‑461644
                                                                                                                                                 |                                                                                                                                                 
agagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                                >  2:1843095/1‑149 (MQ=255)
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                               >  2:3477115/1‑149 (MQ=255)
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGCTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                               >  2:3980330/1‑149 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTg                                                                                                                                              >  1:3447675/1‑149 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTg                                                                                                                                              >  1:579254/1‑149 (MQ=255)
    tataTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGcc                                                                                                                                            >  2:4076514/1‑149 (MQ=255)
     atatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                           >  2:2875365/1‑149 (MQ=255)
     atatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                           <  2:1626449/149‑1 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                          >  2:422788/1‑149 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                          >  2:2878601/1‑149 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                          >  1:3542358/1‑149 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                          <  1:2981102/149‑1 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGt                                                                                                                                       <  1:464542/149‑1 (MQ=255)
          ccGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTa                                                                                                                                      <  1:728699/149‑1 (MQ=255)
           cGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTAt                                                                                                                                     <  2:1917731/149‑1 (MQ=255)
              tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGaa                                                                                                                                  >  1:1966683/1‑149 (MQ=255)
                    gCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATTAAAAATAATTGTGAGTTCAAAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                            >  1:149422/1‑149 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAt                                                                                                                           <  2:1415230/149‑1 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAt                                                                                                                           <  2:3041240/149‑1 (MQ=255)
                      cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATc                                                                                                                          <  2:2451294/149‑1 (MQ=255)
                       aaTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                         >  1:4019880/1‑149 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                       <  2:2495411/149‑1 (MQ=255)
                            ctCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                                                                                                    <  1:3140026/148‑1 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                                                                                                    >  2:618372/1‑149 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                                                                                                    >  2:2684712/1‑149 (MQ=255)
                             tCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATTAAAAATAATTGTGATTTAACAGCCTTCCCCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATTGTATGAAGTTCTATCGGTAGGt                                                                                                                   >  1:1452898/1‑149 (MQ=255)
                                gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTGTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGAt                                                                                                                <  2:152823/149‑1 (MQ=255)
                                    cccATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTTCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAAc                                                                                                            >  1:1800719/1‑149 (MQ=255)
                                    cccATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAAc                                                                                                            <  2:3838416/149‑1 (MQ=255)
                                       atcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAg                                                                                                         >  2:2490900/1‑149 (MQ=255)
                                        tcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGc                                                                                                        >  1:2083729/1‑149 (MQ=255)
                                           tGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCACAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTc                                                                                                     >  2:1051360/1‑149 (MQ=255)
                                             aGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCaa                                                                                                   >  1:3701995/1‑149 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGt                                                                                                >  2:2947227/1‑149 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGt                                                                                                <  1:869429/149‑1 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGt                                                                                                >  2:1330882/1‑149 (MQ=255)
                                                 cTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGtt                                                                                               <  2:2518706/149‑1 (MQ=255)
                                                  tGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGttt                                                                                              >  1:2993560/1‑149 (MQ=255)
                                                   gCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTGTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGtttt                                                                                             <  2:2223993/149‑1 (MQ=255)
                                                    cGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTc                                                                                            >  1:2419417/1‑149 (MQ=255)
                                                     gtgtGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCg                                                                                           <  2:1387103/149‑1 (MQ=255)
                                                      tgtgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATAGGTAGGTGATAAACCAGCGTCAAGGTTTTCgg                                                                                          >  1:1846389/1‑149 (MQ=255)
                                                       gggCCTGGAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGt                                                                                         <  1:3033906/147‑1 (MQ=255)
                                                        tgCCTGGAGGGTCGTGTCGTCCCAGATTTTTGCGTTATGAAAAATAATTGTGAGGTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTa                                                                                        <  1:404852/149‑1 (MQ=255)
                                                          ccTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                      <  1:2626154/149‑1 (MQ=255)
                                                            tGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGttt                                                                                    >  2:1441178/1‑149 (MQ=255)
                                                              tAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGa                                                                                  <  2:1203317/149‑1 (MQ=255)
                                                               aGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGAc                                                                                 >  1:3616521/1‑149 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTTTGAGTTAAAAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACt                                                                                >  1:2138580/1‑149 (MQ=255)
                                                                gggTCGTGTCGTCCCAGTTTTTTGCGTTAAGAAAAATAAATGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTATGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACt                                                                                >  1:3436689/1‑149 (MQ=255)
                                                                  gtcgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCGGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGTCTTc                                                                              >  1:2471181/1‑149 (MQ=255)
                                                                    cgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCAt                                                                            <  2:1580148/149‑1 (MQ=255)
                                                                       gtcgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCg                                                                         <  1:3550077/149‑1 (MQ=255)
                                                                          gtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTa                                                                      <  1:3956803/149‑1 (MQ=255)
                                                                          gtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTa                                                                      >  2:3945158/1‑149 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                    <  1:1710773/149‑1 (MQ=255)
                                                                             ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCt                                                                   >  1:1933293/1‑149 (MQ=255)
                                                                             ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCt                                                                   >  1:1131495/1‑149 (MQ=255)
                                                                                gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                                <  2:944023/149‑1 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCaa                                                              >  1:1203156/1‑149 (MQ=255)
                                                                                   ttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAAt                                                             <  2:1629370/149‑1 (MQ=255)
                                                                                    tttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATg                                                            <  1:3815153/149‑1 (MQ=255)
                                                                                     ttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGc                                                           <  2:499651/149‑1 (MQ=255)
                                                                                      tGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCg                                                          >  2:3378860/1‑149 (MQ=255)
                                                                                       gCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCgg                                                         >  1:3564257/1‑149 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAAt                                                      >  2:3009676/1‑149 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAAt                                                      <  2:3010804/149‑1 (MQ=255)
                                                                                               aaaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                                 <  1:2746721/149‑1 (MQ=255)
                                                                                                   ataatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGcc                                             >  1:4104651/1‑149 (MQ=255)
                                                                                                    taatTGTTAGTTCACAGCATTCCGCTGCCACTGTCGCCTGTAGGCTTTCTGCCATCTTATGAACTTCTATAGGTATGTGATAAACCAGCTTCAAGGTTTTCGGTAGTTTGTCTTCATCCGGTACCTGCCAATGATGAATAGTCCAGCCt                                            >  2:807423/1‑149 (MQ=255)
                                                                                                    taatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCt                                            <  2:3364535/149‑1 (MQ=255)
                                                                                                       ttGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCGATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGc                                         <  1:204319/149‑1 (MQ=255)
                                                                                                        tgtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCa                                        <  1:659791/149‑1 (MQ=255)
                                                                                                         gtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAACCCAGCGTCAAGGTTTTCGGTAGTTTGACCTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAg                                       >  2:3913964/1‑149 (MQ=255)
                                                                                                           gAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTa                                     <  2:672839/149‑1 (MQ=255)
                                                                                                             gTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCCATGCGGAATAGTCCAGCCTGGCAGTAAt                                   >  2:3950259/1‑149 (MQ=255)
                                                                                                             gTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAt                                   >  1:2237274/1‑149 (MQ=255)
                                                                                                              ttCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATg                                  <  2:2083729/149‑1 (MQ=255)
                                                                                                                   aGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGa                             >  2:1273104/1‑149 (MQ=255)
                                                                                                                    gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                            <  1:1330882/149‑1 (MQ=255)
                                                                                                                      cTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTg                          <  2:1104709/149‑1 (MQ=255)
                                                                                                                       ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                         <  1:2363349/149‑1 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                        <  1:2947227/149‑1 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGc                        <  2:2316096/149‑1 (MQ=255)
                                                                                                                         ccGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCGGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCg                       <  2:1847949/149‑1 (MQ=255)
                                                                                                                            cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTg                    >  1:2131826/1‑149 (MQ=255)
                                                                                                                            cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCGATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTg                    <  2:3644647/149‑1 (MQ=255)
                                                                                                                             tGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                   <  1:2878601/149‑1 (MQ=255)
                                                                                                                              gCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGAt                  <  2:1800719/149‑1 (MQ=255)
                                                                                                                               ccAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATc                 >  2:2262866/1‑149 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCa                >  2:3564103/1‑149 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCa                <  1:1830108/149‑1 (MQ=255)
                                                                                                                                  gtgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTACATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg              >  1:16117/1‑149 (MQ=255)
                                                                                                                                   tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGt             <  2:1707570/149‑1 (MQ=255)
                                                                                                                                    gtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtt            <  2:2637548/149‑1 (MQ=255)
                                                                                                                                      cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtttt          <  1:2684712/149‑1 (MQ=255)
                                                                                                                                      cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGtttt          <  1:618372/149‑1 (MQ=255)
                                                                                                                                         cTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtctc       <  1:1025064/149‑1 (MQ=255)
                                                                                                                                             aGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAc   <  2:217492/149‑1 (MQ=255)
                                                                                                                                              ggCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACt  <  1:2699893/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACT  >  CP009273/461354‑461644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑