Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 49,840 G→T 100% A6A (GCG→GCT)  folA → dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927349,8400GT100.0% 780.3 / NA 231A6A (GCG→GCT) folAdihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (124/107);  total (124/107)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

AGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  CP009273/49696‑49984
                                                                                                                                                |                                                                                                                                                
aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAACTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGt                                                                                                                                              <  2:351201/149‑1 (MQ=255)
aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGt                                                                                                                                              <  2:1378617/149‑1 (MQ=255)
   ccAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAg                                                                                                                                           <  1:2795583/149‑1 (MQ=255)
     aGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                         >  2:541066/1‑149 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  2:2313853/1‑149 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  1:278785/1‑149 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  1:2001945/1‑149 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  2:1166102/1‑149 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  2:1843623/1‑149 (MQ=255)
          gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGATGCGTTAGCGGTAGa                                                                                                                                    >  2:4083054/1‑149 (MQ=255)
           agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGAt                                                                                                                                   <  2:626719/149‑1 (MQ=255)
           agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGAt                                                                                                                                   >  2:2488083/1‑149 (MQ=255)
           agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGAt                                                                                                                                   <  2:3703650/149‑1 (MQ=255)
                   gACTCGCCAGCAGAATATAAAATTTTCCTCCACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTAt                                                                                                                           <  2:1080905/149‑1 (MQ=255)
                   gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTAt                                                                                                                           >  2:176693/1‑149 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          <  1:3908269/149‑1 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          >  1:3343709/1‑149 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          >  1:756366/1‑149 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          <  1:2680284/149‑1 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          <  2:895054/149‑1 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          >  2:2695978/1‑149 (MQ=255)
                       cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGc                                                                                                                       <  1:2307455/149‑1 (MQ=255)
                       cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGc                                                                                                                       >  1:2616996/1‑149 (MQ=255)
                         ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCAt                                                                                                                     <  1:2473668/149‑1 (MQ=255)
                            gcagAATATAACATTTTCCGCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGa                                                                                                                  <  2:3785606/149‑1 (MQ=255)
                            gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGa                                                                                                                  <  2:2525879/149‑1 (MQ=255)
                               gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaaa                                                                                                               >  2:1208363/1‑149 (MQ=255)
                               gAATATAAAATTTTCCTCAACAACATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaaa                                                                                                               <  1:3763840/149‑1 (MQ=255)
                                aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                              <  1:992574/149‑1 (MQ=255)
                                aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                              <  2:3848255/149‑1 (MQ=255)
                                 atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                                                                                                             >  1:1851600/1‑149 (MQ=255)
                                 atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                                                                                                             >  2:1672006/1‑149 (MQ=255)
                                 atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                                                                                                             >  1:233108/1‑149 (MQ=255)
                                   ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                                           <  2:3269236/149‑1 (MQ=255)
                                   ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                                           <  1:2198455/149‑1 (MQ=255)
                                      aaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATg                                                                                                        >  1:3037187/1‑149 (MQ=255)
                                        aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGcc                                                                                                      >  2:3391265/1‑149 (MQ=255)
                                          tttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGt                                                                                                    <  2:3107591/149‑1 (MQ=255)
                                               tCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                                               <  1:4022377/149‑1 (MQ=255)
                                                cAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcc                                                                                              <  2:54929/149‑1 (MQ=255)
                                                  aCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGAGCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                            >  2:3590959/1‑149 (MQ=255)
                                                    atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc                                                                                          >  2:708852/1‑149 (MQ=255)
                                                     tcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                         >  1:586153/1‑149 (MQ=255)
                                                      catcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                                                        >  2:3769082/1‑149 (MQ=255)
                                                            cGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtc                                                                                  <  2:1854734/149‑1 (MQ=255)
                                                              cACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtctc                                                                                <  1:1632613/149‑1 (MQ=255)
                                                               aCCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCg                                                                               >  2:65299/1‑149 (MQ=255)
                                                                       acgacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                                                       <  1:2067789/149‑1 (MQ=255)
                                                                       acgacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                                                       <  1:3069400/149‑1 (MQ=255)
                                                                        cgacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa                                                                      >  2:3140192/1‑149 (MQ=255)
                                                                         gacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                     >  2:2706358/1‑149 (MQ=255)
                                                                          acgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa                                                                    >  2:3140841/1‑149 (MQ=255)
                                                                             gTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGc                                                                 >  1:2974701/1‑149 (MQ=255)
                                                                             gTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGc                                                                 >  1:3176603/1‑149 (MQ=255)
                                                                                 aCGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAaca                                                             >  1:3377180/1‑149 (MQ=255)
                                                                                 aCGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAaca                                                             >  2:2225480/1‑149 (MQ=255)
                                                                                   gCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGGTTAAACGCAACAcc                                                           >  2:506746/1‑149 (MQ=255)
                                                                                     tttACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCAGATCTCGCCTGGTTTAAACGCAACACCtt                                                         <  1:2741849/149‑1 (MQ=255)
                                                                                       tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaa                                                       >  2:1260052/1‑149 (MQ=255)
                                                                                         cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                     <  1:2362511/149‑1 (MQ=255)
                                                                                          gTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata                                                    >  1:1318588/1‑149 (MQ=255)
                                                                                           tataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                   >  2:2688734/1‑149 (MQ=255)
                                                                                           tataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                   <  1:1966823/149‑1 (MQ=255)
                                                                                           tataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                   >  1:1557536/1‑149 (MQ=255)
                                                                                             taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                 >  2:2183108/1‑149 (MQ=255)
                                                                                               gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                               >  1:958882/1‑149 (MQ=255)
                                                                                               gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                               >  1:1671523/1‑149 (MQ=255)
                                                                                                 ggCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                             >  1:1016203/1‑149 (MQ=255)
                                                                                                  gCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAACCGTg                                            >  2:915986/1‑149 (MQ=255)
                                                                                                    gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                          >  1:1846748/1‑149 (MQ=255)
                                                                                                      cAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                        <  2:3276310/149‑1 (MQ=255)
                                                                                                       aaTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                       >  2:2435857/1‑149 (MQ=255)
                                                                                                        aTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                      <  2:870036/149‑1 (MQ=255)
                                                                                                        aTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                      <  2:3334877/149‑1 (MQ=255)
                                                                                                         ttttttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                     >  1:2949548/1‑149 (MQ=255)
                                                                                                         ttttttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                     >  2:2837145/1‑149 (MQ=255)
                                                                                                          tttttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                    <  2:4054741/149‑1 (MQ=255)
                                                                                                            tttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                  >  1:3140911/1‑149 (MQ=255)
                                                                                                              tttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                <  2:1559646/149‑1 (MQ=255)
                                                                                                              tttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                <  2:3662909/149‑1 (MQ=255)
                                                                                                                tATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                              >  1:3621918/1‑149 (MQ=255)
                                                                                                                 aTCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                             <  1:708852/149‑1 (MQ=255)
                                                                                                                      gAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg                        <  1:2346811/149‑1 (MQ=255)
                                                                                                                          tctcAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTGATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaa                    <  2:3404272/149‑1 (MQ=255)
                                                                                                                            tcAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatc                  >  1:3238669/1‑149 (MQ=255)
                                                                                                                              aaTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                >  1:2414947/1‑149 (MQ=255)
                                                                                                                               aTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat               >  1:3292423/1‑149 (MQ=255)
                                                                                                                               aTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat               <  1:2695978/149‑1 (MQ=255)
                                                                                                                               aTGATCAGACTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGTCATGGAAAACGCCATGACGTGGAACCTGCATGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAACCGTGATTATGTGCCGCCATACATGGGaatcaat               >  1:246760/1‑149 (MQ=255)
                                                                                                                                 gATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCg             >  1:146605/1‑149 (MQ=255)
                                                                                                                                  aTCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg            >  1:3494676/1‑149 (MQ=255)
                                                                                                                                   tCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTTGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt           <  2:3013814/149‑1 (MQ=255)
                                                                                                                                      gTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgt        >  1:79730/1‑149 (MQ=255)
                                                                                                                                       tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc       <  2:2404282/149‑1 (MQ=255)
                                                                                                                                       tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc       <  2:2369098/149‑1 (MQ=255)
                                                                                                                                          gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt    <  2:2001945/149‑1 (MQ=255)
                                                                                                                                          gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt    <  2:278597/149‑1 (MQ=255)
                                                                                                                                          gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt    <  1:1208363/149‑1 (MQ=255)
                                                                                                                                           aTTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt   <  1:3561525/149‑1 (MQ=255)
                                                                                                                                            ttGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg  >  2:2395979/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  CP009273/49696‑49984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑