Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 479,244 C→A 100% A206S (GCC→TCC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273479,2440CA99.8% 735.5 / ‑6.7 240A206S (GCC→TCC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (115/123);  minor base G (2/0);  total (117/123)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

ATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG  >  CP009273/479098‑479389
                                                                                                                                                  |                                                                                                                                                 
aTTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                                 <  2:662266/149‑1 (MQ=255)
aTTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                                 <  2:2239854/149‑1 (MQ=255)
aTTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                                 <  2:2194288/149‑1 (MQ=255)
aTTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                                 <  1:969568/149‑1 (MQ=255)
 ttCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGt                                                                                                                                                >  2:2127266/1‑149 (MQ=255)
 ttCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGt                                                                                                                                                >  2:1503976/1‑149 (MQ=255)
 ttCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATggtggt                                                                                                                                                >  1:3962107/1‑149 (MQ=255)
 ttCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGt                                                                                                                                                >  1:1876413/1‑149 (MQ=255)
    aCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCATCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAt                                                                                                                                             >  1:1917685/1‑149 (MQ=255)
    aCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCATCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCGGCGCGGTGATGGAGGTTAt                                                                                                                                             >  1:2098099/1‑149 (MQ=255)
     cTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATg                                                                                                                                            >  1:2125133/1‑149 (MQ=255)
      tttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGa                                                                                                                                           >  1:155554/1‑149 (MQ=255)
      tttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGa                                                                                                                                           >  1:3478260/1‑149 (MQ=255)
        tCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACa                                                                                                                                         <  2:4030004/149‑1 (MQ=255)
         cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAt                                                                                                                                        <  2:1161647/149‑1 (MQ=255)
         cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAt                                                                                                                                        <  2:3212184/149‑1 (MQ=255)
          agcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATc                                                                                                                                       <  2:3526913/149‑1 (MQ=255)
                   ttGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGt                                                                                                                              >  1:2885891/1‑149 (MQ=255)
                     gCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTa                                                                                                                            <  1:1409355/149‑1 (MQ=255)
                     gCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTa                                                                                                                            <  2:2606896/149‑1 (MQ=255)
                     gCCGAACTCTTCAGTAGAGGTCACACGCGTCTGAGCAATAATAGAGGCGTTAAGCAGTTGGCCATTCACCGGCGGCGTACCCCCGAGCTGACCCTCCGCAACCAGGCCGTTCTGCGCATTGAGGGAGGTAATGACATCAACCGGCGTTa                                                                                                                            >  2:3864199/1‑149 (MQ=255)
                       cGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGc                                                                                                                          <  1:2653126/149‑1 (MQ=255)
                         aaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTg                                                                                                                        >  1:629755/1‑149 (MQ=255)
                           ctctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGa                                                                                                                      >  1:1731602/1‑149 (MQ=255)
                             ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAt                                                                                                                    >  1:2249072/1‑149 (MQ=255)
                             ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAt                                                                                                                    >  1:1690115/1‑149 (MQ=255)
                             ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAt                                                                                                                    <  2:1266428/149‑1 (MQ=255)
                              ttCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAtt                                                                                                                   <  2:1356915/149‑1 (MQ=255)
                                cAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTg                                                                                                                 <  2:3774058/149‑1 (MQ=255)
                                cAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGGTAGCTGGAATTTg                                                                                                                 >  1:3058908/1‑149 (MQ=255)
                                cAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCCCCGCAACCTGGGCGTTCTGGGCTTTTTTGGAGGTAATTAAAAAAAACCGCGTTATCTGGAAattt                                                                                                                 >  1:375453/1‑148 (MQ=255)
                                 aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAAGACATCAACCGGCGTTAGCTGGAATTTGt                                                                                                                >  2:2719991/1‑149 (MQ=255)
                                   tAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTc                                                                                                              <  2:2661226/149‑1 (MQ=255)
                                     gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAg                                                                                                            >  2:3431006/1‑149 (MQ=255)
                                     gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCAACGGCGGCGTACCACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCGTTTATTGGCGTAAAGACATAAACCCGCCTTAGCTGGAATTTGttaat                                                                                                            >  1:3125031/1‑146 (MQ=255)
                                        gTCAGACGCGTCTGAGAAATAATCGAGGCGTTAAGCTGTTGGACTTTCACCGGCGGCGTACCACCGAGCTGACCCTCCGCATCCTGGGCGTTCTGCTCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTc                                                                                                         <  1:402284/149‑1 (MQ=255)
                                           aGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt                                                                                                      <  1:1503976/149‑1 (MQ=255)
                                           aGACGCGTCTGAGCAATAAGAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt                                                                                                      <  2:2890333/149‑1 (MQ=255)
                                            gACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGGGCTTTGATGGAGGTAATGACATCAACCGGCGGTAGCTGGCAATTGTTAAGCTCCTTc                                                                                                     >  1:788862/1‑149 (MQ=255)
                                               gcgTCTGAGCAATAATAGAGGCGTTAAGCTGTTTGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACAACAACCGGCGTTAGCTGGAAATTTTTCAGCTAAATCggg                                                                                                  >  1:1495991/1‑149 (MQ=255)
                                                 gTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGTTGGAGGTAATGACATCAAACGGCGTTAGCTTGAATTTGTTGAGCTCATTCGGGtt                                                                                                >  1:1790714/1‑149 (MQ=255)
                                                  tCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                               <  1:302338/149‑1 (MQ=255)
                                                  tCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                               <  1:2375487/149‑1 (MQ=255)
                                                  tCTGAGCAATAATAGAGGCGTTAACCTGGTGGCCTTTCAACGGCGGCGTACCACCGAGCTGACCAGCCGCAAACTGGGCGATCTGAGATATGATGGAGGTAATGACATAAACCCTCGTTAGATAGAATTTGTTCAGCTCATTCGGGTTc                                                                                               >  2:1838239/1‑149 (MQ=255)
                                                   cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCTGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCa                                                                                              <  2:3830500/149‑1 (MQ=255)
                                                    tGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTATCTGGAATTTGTTAAGCTCATTCGGGTTCAt                                                                                             >  1:3646109/1‑149 (MQ=255)
                                                      aGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATcc                                                                                           >  1:3258665/1‑149 (MQ=255)
                                                      aGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATcc                                                                                           <  1:185887/149‑1 (MQ=255)
                                                           taataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATa                                                                                      <  2:2167935/149‑1 (MQ=255)
                                                           taataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCACGATa                                                                                      >  1:1238607/1‑149 (MQ=255)
                                                            aataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTTCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAc                                                                                     <  2:239196/149‑1 (MQ=255)
                                                            aataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTTCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAc                                                                                     <  2:238332/149‑1 (MQ=255)
                                                            aataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAc                                                                                     <  1:1786862/149‑1 (MQ=255)
                                                              taGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTAACACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTAAGCTCATTCTGGTTCATCCAGATAAGc                                                                                   >  2:1837217/1‑149 (MQ=255)
                                                                 agGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATc                                                                                <  1:2221628/149‑1 (MQ=255)
                                                                 agGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATc                                                                                <  1:3082911/149‑1 (MQ=255)
                                                                  ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                               >  1:474026/1‑149 (MQ=255)
                                                                  ggCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                               >  2:3255450/1‑149 (MQ=255)
                                                                   gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcgc                                                                              <  2:3581511/149‑1 (MQ=255)
                                                                        aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACt                                                                         >  1:3635691/1‑149 (MQ=255)
                                                                        aaGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACt                                                                         >  2:1387241/1‑149 (MQ=255)
                                                                          gCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgt                                                                       >  1:311309/1‑149 (MQ=255)
                                                                              ttGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAc                                                                   >  1:2928670/1‑149 (MQ=255)
                                                                                ggCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCg                                                                 >  2:88180/1‑149 (MQ=255)
                                                                                  ccTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaa                                                               >  2:3645768/1‑149 (MQ=255)
                                                                                    tttCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaaca                                                             >  2:1694935/1‑149 (MQ=255)
                                                                                      tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaac                                                           <  1:2500935/149‑1 (MQ=255)
                                                                                            gcggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACa                                                     >  1:1015453/1‑149 (MQ=255)
                                                                                               gcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                  >  2:3438799/1‑149 (MQ=255)
                                                                                                cgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCAc                                                 <  1:2999583/149‑1 (MQ=255)
                                                                                                       ccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGcc                                          <  2:77169/149‑1 (MQ=255)
                                                                                                        cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGccc                                         <  2:309905/149‑1 (MQ=255)
                                                                                                          aGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGa                                       <  2:1639454/149‑1 (MQ=255)
                                                                                                            cTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTTCTGTGAACCGAACAACTGAACATCACCCACGCCCGACg                                     >  1:2568596/1‑149 (MQ=255)
                                                                                                             tGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTTTTAAGCTCATTAGGGTTAATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGt                                    >  1:1766148/1‑149 (MQ=255)
                                                                                                             tGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGt                                    >  1:251857/1‑149 (MQ=255)
                                                                                                             tGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGt                                    <  2:2147751/149‑1 (MQ=255)
                                                                                                               aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAc                                  >  1:3561563/1‑149 (MQ=255)
                                                                                                               aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAc                                  >  2:410834/1‑149 (MQ=255)
                                                                                                               aCCCGCCGCAACCTGGGCGTGCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGCATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACAGAACATCACCCACGCCCGACGTAc                                  >  1:3561877/1‑149 (MQ=255)
                                                                                                                 ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACgg                                >  1:343838/1‑149 (MQ=255)
                                                                                                                  cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGc                               <  2:2249072/149‑1 (MQ=255)
                                                                                                                   gccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                              <  1:693596/149‑1 (MQ=255)
                                                                                                                    ccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTg                             <  1:1344032/149‑1 (MQ=255)
                                                                                                                      gcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGAt                           <  1:2782914/149‑1 (MQ=255)
                                                                                                                      gcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGAt                           >  2:491595/1‑149 (MQ=255)
                                                                                                                      gcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGAt                           <  1:2053514/149‑1 (MQ=255)
                                                                                                                       cAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                          <  2:3376615/149‑1 (MQ=255)
                                                                                                                        aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATgg                         >  2:683965/1‑149 (MQ=255)
                                                                                                                           cTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAt                      <  2:2290614/149‑1 (MQ=255)
                                                                                                                            tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATc                     <  1:2276061/149‑1 (MQ=255)
                                                                                                                              ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCtt                   <  2:2966865/149‑1 (MQ=255)
                                                                                                                              ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCtt                   <  2:2883526/149‑1 (MQ=255)
                                                                                                                               gCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                  <  1:3407702/149‑1 (MQ=255)
                                                                                                                               gCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                  <  1:2845276/149‑1 (MQ=255)
                                                                                                                                cGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTc                 <  2:1905865/149‑1 (MQ=255)
                                                                                                                                  ttCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat               >  1:1564569/1‑149 (MQ=255)
                                                                                                                                      gcgcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTc           >  2:2993027/1‑149 (MQ=255)
                                                                                                                                         cTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgcc        <  2:3868587/149‑1 (MQ=255)
                                                                                                                                              aTGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGTACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCAc   >  1:3434142/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG  >  CP009273/479098‑479389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑