Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 481,667 G→A 100% A151T (GCG→ACG)  acrR → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273481,6670GA99.6% 817.3 / ‑4.5 243A151T (GCG→ACG) acrRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (139/103);  total (140/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

AGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481522‑481812
                                                                                                                                                 |                                                                                                                                                 
agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGa                                                                                                                                                <  2:3260172/149‑1 (MQ=255)
    gaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACTGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAt                                                                                                                                            >  1:2037327/1‑149 (MQ=255)
       cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGtt                                                                                                                                         <  2:182731/149‑1 (MQ=255)
         gCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACACACGTTAAAACATTGTATTGAAACGAAAATGTTGc                                                                                                                                       <  1:1471174/149‑1 (MQ=255)
         gCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGc                                                                                                                                       <  1:2795875/149‑1 (MQ=255)
            tCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                    >  2:148959/1‑149 (MQ=255)
            tCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                    >  1:4098026/1‑149 (MQ=255)
                ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGa                                                                                                                                >  1:1977644/1‑149 (MQ=255)
                  attGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAtt                                                                                                                              <  1:1212379/149‑1 (MQ=255)
                   ttGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAttt                                                                                                                             >  1:2262079/1‑149 (MQ=255)
                      aTGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTACTCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAt                                                                                                                          <  2:2122132/149‑1 (MQ=255)
                      aTGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAt                                                                                                                          <  2:1145834/149‑1 (MQ=255)
                      aTGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAt                                                                                                                          <  2:3977839/149‑1 (MQ=255)
                       tGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATg                                                                                                                         >  2:37322/1‑149 (MQ=255)
                         gagaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAc                                                                                                                       >  1:3567792/1‑149 (MQ=255)
                           gaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACCAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     <  2:352008/149‑1 (MQ=255)
                           gaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     <  2:3810310/149‑1 (MQ=255)
                           gaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     >  1:228988/1‑149 (MQ=255)
                            aTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgcg                                                                                                                    >  2:1923308/1‑149 (MQ=255)
                            aTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgcg                                                                                                                    >  2:3356657/1‑149 (MQ=255)
                             ttATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGt                                                                                                                   >  2:529054/1‑149 (MQ=255)
                              tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTc                                                                                                                  >  2:1223961/1‑149 (MQ=255)
                              tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTc                                                                                                                  <  1:3638346/149‑1 (MQ=255)
                                tatTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcgc                                                                                                                >  1:1421973/1‑149 (MQ=255)
                                tatTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcgc                                                                                                                >  1:1345425/1‑149 (MQ=255)
                                  ttCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCgcgc                                                                                                              >  1:2714489/1‑149 (MQ=255)
                                  ttCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCgcgc                                                                                                              >  1:2780858/1‑149 (MQ=255)
                                   tCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgca                                                                                                             <  2:1548116/149‑1 (MQ=255)
                                   tCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgca                                                                                                             >  2:2019407/1‑149 (MQ=255)
                                       caAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCaa                                                                                                         >  1:2587945/1‑149 (MQ=255)
                                       caAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCaa                                                                                                         >  1:2266487/1‑149 (MQ=255)
                                        aaaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAat                                                                                                        <  1:696972/149‑1 (MQ=255)
                                        aaaGGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAat                                                                                                        <  1:2183615/149‑1 (MQ=255)
                                           tGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAATAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     >  2:1245007/1‑149 (MQ=255)
                                           tGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     >  1:595565/1‑149 (MQ=255)
                                           tGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     >  2:3607537/1‑149 (MQ=255)
                                             cGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAAttatta                                                                                                   >  2:3581047/1‑149 (MQ=255)
                                                aTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgc                                                                                                >  2:2432669/1‑149 (MQ=255)
                                                 tttGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgcg                                                                                               >  1:2402724/1‑149 (MQ=255)
                                                    gTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                            >  1:1252617/1‑149 (MQ=255)
                                                    gTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGACTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                            >  1:3476190/1‑149 (MQ=255)
                                                      cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAATAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                          <  2:3317974/149‑1 (MQ=255)
                                                      cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                          >  1:100404/1‑149 (MQ=255)
                                                      cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                          >  1:1695163/1‑149 (MQ=255)
                                                       ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCta                                                                                         <  1:6568/149‑1 (MQ=255)
                                                       ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCta                                                                                         <  1:3355176/149‑1 (MQ=255)
                                                        gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAATAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  1:2777235/1‑149 (MQ=255)
                                                        gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  1:898598/1‑149 (MQ=255)
                                                          gaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTatat                                                                                      >  2:1863559/1‑149 (MQ=255)
                                                             aTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTc                                                                                   <  2:1056019/149‑1 (MQ=255)
                                                              tGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                  >  2:1026280/1‑149 (MQ=255)
                                                                gCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCgg                                                                                >  2:1474656/1‑149 (MQ=255)
                                                                  tgttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGcc                                                                              >  1:576371/1‑149 (MQ=255)
                                                                      gtgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGAt                                                                          <  2:3201294/149‑1 (MQ=255)
                                                                           aCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaa                                                                     >  2:3791951/1‑149 (MQ=255)
                                                                            cAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaaa                                                                    >  2:2337242/1‑149 (MQ=255)
                                                                                    aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctct                                                            >  2:4023611/1‑149 (MQ=255)
                                                                                     cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                           <  2:2801557/149‑1 (MQ=255)
                                                                                       tAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                         >  2:692147/1‑149 (MQ=255)
                                                                                        aaTCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGc                                                        <  1:37175/149‑1 (MQ=255)
                                                                                               gtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                 >  1:4048545/1‑149 (MQ=255)
                                                                                                  tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  2:1394769/149‑1 (MQ=255)
                                                                                                  tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  1:3686239/149‑1 (MQ=255)
                                                                                                  tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  1:2432669/149‑1 (MQ=255)
                                                                                                   ggAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                             >  1:798865/1‑149 (MQ=255)
                                                                                                    gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                            >  2:1747674/1‑149 (MQ=255)
                                                                                                      aaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTg                                          >  2:273661/1‑149 (MQ=255)
                                                                                                       aGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGa                                         >  1:3020985/1‑149 (MQ=255)
                                                                                                          tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCt                                      >  2:770768/1‑149 (MQ=255)
                                                                                                          tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAAAGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTATTGATCt                                      >  2:2690988/1‑149 (MQ=255)
                                                                                                           aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     >  1:1336676/1‑149 (MQ=255)
                                                                                                           aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     >  1:3048387/1‑149 (MQ=255)
                                                                                                            tGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTa                                    >  1:2694213/1‑149 (MQ=255)
                                                                                                             gACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAAGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaa                                   >  2:3978863/1‑149 (MQ=255)
                                                                                                              aCCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaa                                  >  1:1524216/1‑149 (MQ=255)
                                                                                                                 gTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaaaa                               <  2:2835657/149‑1 (MQ=255)
                                                                                                                 gTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaaaa                               >  1:3904191/1‑149 (MQ=255)
                                                                                                                    taGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaa                            >  2:3018500/1‑149 (MQ=255)
                                                                                                                     aGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaag                           >  2:3431851/1‑149 (MQ=255)
                                                                                                                       aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGcc                         <  2:2482224/149‑1 (MQ=255)
                                                                                                                       aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGcc                         <  2:2896797/149‑1 (MQ=255)
                                                                                                                        aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                        >  1:500395/1‑149 (MQ=255)
                                                                                                                        aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                        <  2:3092263/149‑1 (MQ=255)
                                                                                                                          aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      <  2:3483799/149‑1 (MQ=255)
                                                                                                                          aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      >  1:2481456/1‑149 (MQ=255)
                                                                                                                           aaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgcg                     >  1:3751351/1‑149 (MQ=255)
                                                                                                                                tAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                >  2:2367287/1‑149 (MQ=255)
                                                                                                                                 aaaaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACg               >  1:730754/1‑149 (MQ=255)
                                                                                                                                 aaaaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGACCGCGATTACg               >  2:2993092/1‑149 (MQ=255)
                                                                                                                                  aaaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGt              >  1:1057262/1‑149 (MQ=255)
                                                                                                                                   aaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGcc                         >  2:1339955/1‑137 (MQ=255)
                                                                                                                                    aCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTg            >  1:1106402/1‑149 (MQ=255)
                                                                                                                                     cATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGc           >  2:2243433/1‑149 (MQ=255)
                                                                                                                                      aTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGcc          >  1:2903119/1‑149 (MQ=255)
                                                                                                                                      aTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGcc          <  2:3872903/149‑1 (MQ=255)
                                                                                                                                       ttGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCa         <  1:1589602/149‑1 (MQ=255)
                                                                                                                                            ttGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTa    >  2:2592545/1‑149 (MQ=255)
                                                                                                                                             tGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc   <  2:3714122/149‑1 (MQ=255)
                                                                                                                                             tGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc   <  2:1408110/149‑1 (MQ=255)
                                                                                                                                              gAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACt  >  2:2067821/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481522‑481812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑