BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	490,268	A→T	46.9%	K136N (AAA→AAT)	recR →	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	490,268	0	A	T	46.9%	62.4 / inf	217	K136N (AAA→AAT)	recR	gap repair protein
Reads supporting (aligned to +/- strand): ref base A (53/62); new base T (52/50); total (105/112)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

AGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGAC  >  CP009273/490120‑490415

agagTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATTGGGCCTCGGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGaaaaa                                                                                                                                                     >  1:1858888/1‑149 (MQ=255)

    tCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCa                                                                                                                                                 <  1:3254373/149‑1 (MQ=255)

       ggctgACATCTACGACATTGAGGAGTAGGGTCAGTTTTCTGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGTCATCGGTCCGGATGATATCGGGCTTGATCTTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTg                                                                                                                                              <  1:3680640/149‑1 (MQ=255)

       ggcggACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTg                                                                                                                                              <  1:2172369/149‑1 (MQ=255)

        gcggACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGa                                                                                                                                             <  1:2338768/149‑1 (MQ=255)

         cggACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGaa                                                                                                                                            <  1:142388/149‑1 (MQ=255)

         cggACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGaa                                                                                                                                            <  1:2740839/149‑1 (MQ=255)

          ggACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAg                                                                                                                                           <  1:1425802/149‑1 (MQ=255)

           gACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGt                                                                                                                                          <  2:3553604/149‑1 (MQ=255)

            aCATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTg                                                                                                                                         <  1:46653/149‑1 (MQ=255)

              aTCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGAt                                                                                                                                       >  2:2374966/1‑149 (MQ=255)

               tCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATc                                                                                                                                      <  1:4089070/149‑1 (MQ=255)

               tCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCCCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGTACAGCGTCTGGCAGAGGAAAAAATAACTGAAGTGAtg                                                                                                                                      >  2:1753789/1‑148 (MQ=255)

                cTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATcc                                                                                                                                     <  1:799394/149‑1 (MQ=255)

                cTACGCCATGGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATcc                                                                                                                                     <  2:3101232/149‑1 (MQ=255)

                 tACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCt                                                                                                                                    <  2:2036556/149‑1 (MQ=255)

                    gCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTGATTTTGGGTTGGTGGGGCAACTGTCCCCGCGGGACGGCCTCGGTGCGGATGATATCGGGCGTGATCGTCTGGTACAGCGTGTGGGCGAGGGAAATATCACAGAAGTGATGCTAGc                                                                                                                                 >  1:813542/1‑149 (MQ=255)

                     ccATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGcc                                                                                                                                <  2:1423656/149‑1 (MQ=255)

                      cATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATAACTGAAGTGATCCTCGcca                                                                                                                               >  2:1195942/1‑149 (MQ=255)

                      cATTGAGCAGACGGGGCAGTTTTCAGGTCGTGATTTTGTTTTGTTTGGGCATCTGTCACCGCAGGACGGCATAGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCCGAGGAAAAAATCACTGTATTGATCCTCGcca                                                                                                                               >  1:869203/1‑149 (MQ=255)

                              aGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATAACTGAAGTGATCCTCGCCACCAAcccc                                                                                                                       >  2:3426362/1‑149 (MQ=255)

                              aGACGGGGCAGTTTTCAGGTCGTTATTTTGTGGTGCTGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAAcccc                                                                                                                       <  2:2950959/149‑1 (MQ=255)

                               gACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCa                                                                                                                      <  2:309515/149‑1 (MQ=255)

                                   gggCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCGTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATAACTGAAGTGATCCTCGCCACCAACCCCACCGt                                                                                                                  >  1:3385621/1‑149 (MQ=255)

                                    ggCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGtt                                                                                                                 <  2:324070/149‑1 (MQ=255)

                                    ggCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGtt                                                                                                                 >  2:1707489/1‑149 (MQ=255)

                                       aGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGaa                                                                                                              <  2:2708528/149‑1 (MQ=255)

                                       aGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGaa                                                                                                              <  2:646342/149‑1 (MQ=255)

                                        gTTTTCAGGTCGTTATTTTTTGTTGATTGGGCATCAGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATACTCGCCACCAACCCCACGGTTGAAg                                                                                                             >  1:2237986/1‑149 (MQ=255)

                                        gTTTTCAGGTCGTTATTTTTTGTTGATGGGGCATCTGTCACCGCTGGACGGCAAAGGTCCGGATGATATAGGGCTTGATAGTCTGGAACAGCGTCTGGCAGCGGAAAATATAAAAGAAGTGATACTCGCCACCAACCCCACGGTTGAAg                                                                                                             >  1:162534/1‑149 (MQ=255)

                                        gTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAg                                                                                                             <  2:193642/149‑1 (MQ=255)

                                           ttCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTg                                                                                                          >  2:675598/1‑149 (MQ=255)

                                              aGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAg                                                                                                       <  1:3045078/149‑1 (MQ=255)

                                                 tCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTa                                                                                                    <  2:3685379/149‑1 (MQ=255)

                                                    ttATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCg                                                                                                 >  1:1286025/1‑149 (MQ=255)

                                                    ttATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCT‑GAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGc                                                                                                <  2:334247/149‑1 (MQ=255)

                                                     tATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGc                                                                                                <  1:2000228/149‑1 (MQ=255)

                                                      aTTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCt                                                                                               >  2:536279/1‑149 (MQ=255)

                                                       ttttGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTa                                                                                              >  1:2767188/1‑149 (MQ=255)

                                                        tttGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTaa                                                                                             <  2:860717/149‑1 (MQ=255)

                                                        tttGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTaa                                                                                             >  2:2555166/1‑149 (MQ=255)

                                                         ttGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAAc                                                                                            >  2:1325585/1‑149 (MQ=255)

                                                            tgtTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTAc                                                                                         <  1:1579231/149‑1 (MQ=255)

                                                            tgtTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTAc                                                                                         >  1:2995631/1‑149 (MQ=255)

                                                                gATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTg                                                                                     >  1:3296395/1‑149 (MQ=255)

                                                                 aTGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGc                                                                                    <  2:1876470/149‑1 (MQ=255)

                                                                  tGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGcc                                                                                   >  1:2308302/1‑149 (MQ=255)

                                                                       cATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCt                                                                              <  1:2686573/149‑1 (MQ=255)

                                                                         tCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCttt                                                                            >  2:551124/1‑149 (MQ=255)

                                                                         tCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCttt                                                                            >  2:2857965/1‑149 (MQ=255)

                                                                              cACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTgcgcg                                                                       >  2:2098518/1‑149 (MQ=255)

                                                                               aCCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTgcgcgc                                                                      >  1:1063175/1‑149 (MQ=255)

                                                                                 cGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCaa                                                                    <  1:2555166/149‑1 (MQ=255)

                                                                                 cGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCaa                                                                    <  1:3818867/149‑1 (MQ=255)

                                                                                  gCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAat                                                                   <  1:2491752/149‑1 (MQ=255)

                                                                                    tGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAatat                                                                 >  1:3103831/1‑149 (MQ=255)

                                                                                      gACGGCATCGGTCAGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGATGAAAATATCACTGAAGTGATCCTCGCCACCAACCGCACGGTTGTAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGa                                                               <  1:3827845/149‑1 (MQ=255)

                                                                                          gCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTg                                                           >  1:1338497/1‑149 (MQ=255)

                                                                                          gCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTg                                                           >  2:3837548/1‑149 (MQ=255)

                                                                                               ggTCCGGATGATATCGGGCTTGATCGTCTGAAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAagc                                                      <  2:3011417/149‑1 (MQ=255)

                                                                                                gTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAagcc                                                     >  2:159101/1‑149 (MQ=255)

                                                                                                    ggATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAagccagcc                                                 >  2:2149866/1‑149 (MQ=255)

                                                                                                    ggATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAagccagcc                                                 >  2:1561902/1‑149 (MQ=255)

                                                                                                    ggATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAagccagcc                                                 <  1:552552/149‑1 (MQ=255)

                                                                                                     gatgatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCg                                                >  2:2479762/1‑149 (MQ=255)

                                                                                                      atgatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGa                                               >  2:4049940/1‑149 (MQ=255)

                                                                                                      atgatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGa                                               >  2:4022798/1‑149 (MQ=255)

                                                                                                       tgatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGaa                                              <  2:2298117/149‑1 (MQ=255)

                                                                                                        gatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAAt                                             >  2:737738/1‑149 (MQ=255)

                                                                                                        gatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAAt                                             <  2:3730349/149‑1 (MQ=255)

                                                                                                        gatATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAAt                                             <  1:1566289/149‑1 (MQ=255)

                                                                                                          tatCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCg                                           >  2:110524/1‑149 (MQ=255)

                                                                                                            tCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTCTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCt                                         >  1:2773578/1‑149 (MQ=255)

                                                                                                              gggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCa                                       >  2:1971629/1‑149 (MQ=255)

                                                                                                              gggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCCCa                                       >  2:2054583/1‑149 (MQ=255)

                                                                                                              gggCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCa                                       <  2:103727/149‑1 (MQ=255)

                                                                                                                  ttGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGc                                   >  2:3042489/1‑149 (MQ=255)

                                                                                                                    gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCAAGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGt                                 <  1:3952707/149‑1 (MQ=255)

                                                                                                                    gATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCAAGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGt                                 <  1:3130188/149‑1 (MQ=255)

                                                                                                                     aTCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGtt                                <  1:1489804/149‑1 (MQ=255)

                                                                                                                           tGGAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGtt                          >  1:782695/1‑149 (MQ=255)

                                                                                                                             gAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTgg                        >  1:1679385/1‑149 (MQ=255)

                                                                                                                             gAACAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTgg                        <  2:2582110/149‑1 (MQ=255)

                                                                                                                              aaCAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTggc                       <  2:3287415/149‑1 (MQ=255)

                                                                                                                              aaCAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTggc                       >  1:3280769/1‑149 (MQ=255)

                                                                                                                              aaCAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTggc                       <  1:3363989/149‑1 (MQ=255)

                                                                                                                                cAGCGTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTggcgg                     >  1:2334805/1‑149 (MQ=255)

                                                                                                                                cAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTggcgg                     <  1:2903191/149‑1 (MQ=255)

                                                                                                                                    gTCTGGCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGTCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAg                 >  2:3902923/1‑149 (MQ=255)

                                                                                                                                       tGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACCTTGCCGAGCTGTGCGCGCAATCTGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTg              >  2:3585281/1‑149 (MQ=255)

                                                                                                                                       tGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTg              >  2:1617899/1‑149 (MQ=255)

                                                                                                                                        ggCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTgg             >  1:486539/1‑149 (MQ=255)

                                                                                                                                         gCAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGa            >  1:206524/1‑149 (MQ=255)

                                                                                                                                          cAGAGGAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGaa           <  1:3503030/149‑1 (MQ=255)

                                                                                                                                             agGAAAAAATCACTGCAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATg        <  2:2741533/149‑1 (MQ=255)

                                                                                                                                             agGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATg        <  2:786838/149‑1 (MQ=255)

                                                                                                                                               gAAAATATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCAAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGt      >  2:3615834/1‑149 (MQ=255)

                                                                                                                                               gAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGt      <  1:1911186/149‑1 (MQ=255)

                                                                                                                                               gAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGt      <  1:1911476/149‑1 (MQ=255)

                                                                                                                                                   aaaTCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGAc  >  1:788336/1‑149 (MQ=255)

AGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGTGTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAGAGGAAAAAATCACTGAAGTGATCCTCGCCACCAACCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGAC  >  CP009273/490120‑490415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`